Avaliação algimétrica por estímulo nociceptivo térmico e pressórico em cães pré-tratados com levomepromazina, midazolam e quetamina associados ou não ao butorfanol ou buprenorfina

This study aimed at quantifies the pain in dogs under dissociative anesthesia, across thermal and pressoric stimulus and quantify the reasonable period between two different opioids analgesics. In this study, 30 dogs were used and, divided into three groups of 10 animals each, in which the animals of GI received methotrimeprazine and midazolam put on the same syringe with ketamine. The animals of GII received the same treatment of GI but associated with butorphanol and finally the animals of GIII received the same treatment of GI but associated with buprenorphine. The routine parametric evaluations has been proceeded, although using the thermo algimetry measured in degrees C with the average of 52 degrees C and the pressoric algimetry in Kg. In the thermo algimetry, there has been significant difference in GI at the moments M0, M1, M4 and M5; in GII it was found at M0, M1, M5 and M6 and in GIII it was observed the significant at M0 and M1. It has also been shown in pressoric algimetry significant difference in GI at the moments M0, M2 and M3. Among GII it has observed significant difference at all moments and it has found at M0, M9 in GIII. Thus, it has observed significant differences between all groups; for such the M2 of GII smaller than the others; and M4, M5 of GIII bigger than GI and GII. In the assessment of all periods it was observed significant latent period bigger in GI, however, with reasonable period and short recovery in GII and GIII. In the order hand, the postural tonus recovery it was longer in GIII, followed by GII and finally GI. The used method for the measurement of algic stimulus was efficient, noticing a reasonable analgesic period of 3 hours for butorphanol and 6 hours for buprenorphine.

Variações diurnas da temperatura corporal, proteínas plasmáticas e eritrograma de cabras não prenhes

Hemogram values may have variations caused by intrinsic or extrinsic factors, which can take healthy animals to present values outside of the normal reference ranges and it is important to the veterinarian to know these variation factors for a correct results interpretation. The influence of the period of the day is among them. The objective of this study was to test the hypothesis that there are hematological parameters variations, such as erythrogram and plasmatic proteins during the day. The study was carried out with 10 non-pregnant adult goats in Araçatuba/SP/Brazil. The blood sampling and physical exam occurred at three moments between 07:00 and 08:00 a.m. (M1), 12:30 and 01:30 p.m. (M2), 5:00 and 6:00 p.m. (M3). We noted differences in the values of packed cell volume (PCV), hemoglobin, and rectal temperature through the moments, with reduction of PCV and hemoglobin were related to increase in rectal and air temperature, leading to water intake increase and, consequently, decreasing blood concentration. These variations could lead to an error of interpretation of the results due to values below the reference ranges.

Influência do butorfanol sobre os períodos de latência e de ação da ropivacaína pela via peridural na ovariossalpingo-histerectomia em cadelas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos agudos do exercício resistido, aeróbio contínuo e intervalado na pressão intraocular de indivíduos fisicamente ativos e normotensos

Apesar de pouco explorado, a prática de atividade física promove redução da Pressão Intraocular (PIO). O objetivo foi verificar o efeito de diferentes tipos de exercício na PIO. Quinze voluntários foram submetidos a 3 sessões de 30min de exercícios resistidos (3 x 8 repetições a 80%1RM), aeróbio contínuo (60% da Frequência Cardíaca de Reserva [FCR]), aeróbio intervalado (2min a 50% alternando com 1min a 80% da FCR). PIO foi mensurada antes (M1), durante (M2-15min), imediatamente após a sessão (M3) e na recuperação (5min [R1] e 10min [R2]). Como procedimento estatístico foi utilizado ANOVA. Houve redução da PIO nas 3 sessões de exercício (M2 e M3). Na recuperação, PIO permaneceu reduzida após 5min (R1) em todos os modelos. Porém, aos 10min (R2) estava menor que M1 apenas na sessão de exercício intervalado. Os resultados sugerem que o exercício intervalado é mais efetivo que o contínuo e resistido na redução da PIO.

Efeito agudo do exercício resistido, aeróbio contínuo e intervalado na pressão intraocular de indivíduos fisicamente ativos

Estudos sugerem que a atividade física promove redução e contribui no controle da Pressão Intraocular (PIO). O objetivo foi verificar o efeito de diferentes exercícios na PIO. Quinze voluntários foram submetidos a 3 sessões de 30min de exercícios resistidos (3 x 8 repetições a 80%1RM), aeróbio contínuo (60% da Frequência Cardíaca de Reserva [FCR]), aeróbio intervalado (2min a 50% alternando com 1min a 80% da FCR). PIO foi mensurada antes (M1), durante (M2-15min), imediatamente após a sessão (M3) e na recuperação (5min [R1] e 10min [R2]). Adotou-se ANOVA para tratamento estatístico. Houve redução significativa da PIO nas 3 sessões de exercício (M2 e M3). Na recuperação, PIO permaneceu reduzida após 5min (R1) em todos os modelos. Porém, aos 10min (R2) estava menor que M1 apenas na sessão de exercício intervalado. Os resultados sugerem que o exercício intervalado pode ser mais efetivo que o contínuo e resistido na diminuição da PIO.

Caracterização biofísica da interação entre a proteína M2-1 do Vírus Sincicial Respiratório Humano (HRSV) com quercetina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação convencional e estesiômetro: resultados controversos na avaliação sensitiva no câncer de mama

This study, clinical, prospective, randomized study was conducted from August 2004 to February 2008 in a convenience sample of 60 women who underwent breast surgery with axillary dissection, divided into two groups (n = 30). The GI (Guidance Kit with different textures, to perform at home) and GII (control). The objective was to analyze the effect of home orientation in patients after surgery for breast cancer complaining of numbness, evaluation, and the conventional esthesiometer. The surface sensitivity was assessed by monofilament Semmes-Weinstein and evaluation using conventional two test tubes with hot water (38 to 43o C) and cold (16 to 27o C), paintbrush, needle. The GI was subjected to 10 sessions and assessments in both 3 times. The M1 (pre intervention), the M2 (post-intervention) after 10 sessions of physical therapy intervention and M3 (washout) after 3 months the second time for assessment. The region targeted for evaluation and intervention was the sensory nerve dermatome intercostobrachial. In the test of Goodman (conventional assessment) there was improvement in both groups, the P1, during the thermal evaluation. The t-test of student (esthesiometer) there was improvement in P2 only in GI (p = 0.003) between points 1 and 2 while the time 3 (p = 0.121 and p = 0.733 respectively). It was concluded that there were divergent and opposite results after examining the effect of home orientation in the nerve dermatome intercostobrachial, evaluation, and the conventional esthesiometer.

Recria de Rã touro (Rana catesbeiana) em tanques-rede alojados em viveiros de tilápia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito do benzoato de estradiol na recuperação de oócitos, embriões e nos parâmetros hematológicos de cadelas recém acasaladas

We investigated estradiol benzoate effects on oocytes/embryos recovery rate and the influence of this drug on the hematopoietic system. Twenty four bitches were divided in two groups, Group I, 12 females that received a single shot of estradiol benzoate, 0.2 mg/kg intramuscularly, between 2 and 7 days after the date of the last mismating or insemination and, Group II (control), 12 bitches that received 0.2 ml/kg of oily diluent, in corresponding dates. The bitches were ovary-hysterectomized and the uterus/oviduct were isolated and flushed with a PBS, heparin and polyvinyl alcohol solution. Oocytes and embryos were quantified and classified according to their stage of development. Blood counts were performed on M1 (before drug administration), M2 (15 days after drug administration) and M3 (40 days after drug administration). Pearson correlation coefficient was used to analyze the variable retrieval structures, while Fisher exact test was used for the analysis of embryonic viability. ANOVA was used to analyze repeated measurements and Tukey test for hematological parameters. All tests were performed at 5% significance level. The recovery rate of total structures in group I was lower (22.88%) than group II (65.85%). A lower embryo recovery (ratio 3: 52) rate and a greater number of degenerated structures (ratio 11: 1) were observed in group I. Hematological parameters showed significant difference in erythrocytes, hematocrit and hemoglobin concentrations 15 days after drug administration and difference in leukocytes concentration 40 days after using the medication in bitches of group I, however, at the end of the experiment all bitches had blood counts considered normal.

Perfil bioquímico sérico de bezerros bubalinos no período neonatal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Harriet P. Lynch Letters - Accession 16 - M2 (2)

The Harriet P. Lynch Letters consist of correspondence from Harriet P. Lynch to Mrs. Julian B. Salley discussing the equal pay for equal work controversy at Winthrop College (1915-1920) where certain women teachers resigned or were fired. Mrs. Salley and Mrs. Lynch served as president and vice-president respectively of the Equal Suffrage League.

Evidence of the Importance of the First Intracellular Loop of Prokineticin Receptor 2 in Receptor Function

Prokineticin receptors (PROKR) are G protein-coupled receptors (GPCR) that regulate diverse biological processes, including olfactory bulb neurogenesis and GnRH neuronal migration. Mutations in PROKR2 have been described in patients with varying degrees of GnRH deficiency and are located in diverse functional domains of the receptor. Our goal was to determine whether variants in the first intracellular loop (ICL1) of PROKR2 (R80C, R85C, and R85H) identified in patients with hypogonadotropic hypogonadism interfere with receptor function and to elucidate the mechanisms of these effects. Because of structural homology among GPCR, clarification of the role of ICL1 in PROKR2 activity may contribute to a better understanding of this domain across other GPCR. The effects of the ICL1 PROKR2 mutations on activation of signal transduction pathways, ligand binding, and receptor expression were evaluated. Our results indicated that the R85C and R85H PROKR2 mutations interfere only modestly with receptor function, whereas the R80C PROKR2 mutation leads to a marked reduction in receptor activity. Cotransfection of wild-type (WT) and R80C PROKR2 showed that the R80C mutant could exert a dominant negative effect on WT PROKR2 in vitro by interfering with WT receptor expression. In summary, we have shown the importance of Arg80 in ICL1 for PROKR2 expression and demonstrate that R80C PROKR2 exerts a dominant negative effect on WT PROKR2. (Molecular Endocrinology 26: 1417-1427, 2012)

PD controller synthesis from open-loop response measurements of rotating system

In this work, a method of computing PD stabilising gains for rotating systems is presented based on the D-decomposition technique, which requires the sole knowledge of frequency response functions. By applying this method to a rotating system with electromagnetic actuators, it is demonstrated that the stability boundary locus in the plane of feedback gains can be easily plotted, and the most suitable gains can be found to minimise the resonant peak of the system. Experimental results for a Laval rotor show the feasibility of not only controlling lateral shaft vibration and assuring stability, but also helps in predicting the final vibration level achieved by the closed-loop system. These results are obtained based solely on the input-output response information of the system as a whole.

Heritability Estimate and Genetic Correlations of Reproductive Features in Nellore Bulls, Offspring of Super Precocious, Precocious and Normal Cows Under Extensive Farming Conditions

The present work aimed to estimate heritability and genetic correlations of reproductive features of Nellore bulls, offspring of mothers classified as superprecocious (M1), precocious (M2) and normal (M3). Twenty one thousand hundred and eighty-six animals with average age of 21.29 months were used, evaluated through the breeding soundness evaluation from 1999 to 2008. The breeding soundness features included physical semen evaluation (progressive sperm motility and sperm vigour), semen morphology (major, minor and total sperm defects), scrotal circumference (SC), testicular volume (TV) and SC at 18 months of age (SC18). The components of variance, heritability and genetic correlations for and between the features were estimated simultaneously by restricted maximum likelihood, with the use of the vce software system vs 6. The heritability estimates were high for SC18, SC and TV (0.43, 0.63 and 0.54; 0.45, 0.45 and 0.44; 0.42, 0.45 and 0.41, respectively for the categories of mothers M1, M2 and M3) and low for physical and morphological semen aspects. The genetic correlations between SC18 and SC were high, as well as between these variables with TV. High and positive genetic correlations were recorded among SC18, SC and TV with the physical aspects of the semen, although no favourable association was verified with the morphological aspects, for the three categories of mothers. It can be concluded that the mothers sexual precocity did not affect the heritability of their offspring reproduction features.