Entre Arlt y Puig, el affaire Correas. Acerca de "La narración de la historia"

El cuento "La narración de la historia" de Carlos Correas (1931-2000) se publicó en la Revista Centro, en 1959, y provocó un escándalo que llevó al secuestro de lo que sería el último número de esta publicación por parte de la Justicia. Correas había incurrido en una flagrante transgresión a los límites de lo decible en el discurso social de fines de la década del 50, inmerso en lo que Oscar Terán ha denominado "proceso de modernización cultural posperonista". En este trabajo examino el relato de Correas como emergencia de una subjetividad novedosa que permitiría establecer un puente entre la dimensión socio-sexual y estética del mundo ficcional de Arlt y la que unos años más tarde aparecerá configurada en la narrativa de Manuel Puig

La ciudadanía política de las mujeres y las elecciones de 1951

Este estudio analiza los derechos políticos de las mujeres en la Argentina después de la ley 13010 (1947) que les permitió acceder al voto y se electas. El propósito del artí culo es comprender tres diferentes dimensiones de la ciudadanía política. Primero, se enfoca en un grupo de mujeres que votaron por primera vez en 1951 con el objetivo de comprender sus ideas sobre su nuevo status. Segundo, se analiza el comportamiento electoral de varones y mujeres en las urnas en 1951. Tercero, se explora cómo Eva Perón influenció la percepción de la representación y los límites del poder femenino en las relaciones políticas.

(Table 2) Distribution of alkenone unsaturation index UK37 of the eastern North Atlantic

This paper reports the concentrations and within-class distributions of long-chain alkenones and alkyl alkenoates in the surface waters (0-50 m) of the eastern North Atlantic, and correlates their abundance and distribution with those of source organisms and with water temperature and other environmental variables. We collected these samples of >0.8 µm particulate material from the euphotic zone along the JGOFS 20°W longitude transect, from 61°N to 24°N, during seven cruises of the UK-JGOFS Biogeochemical Ocean Flux Study (BOFS) in 1989-1991; the biogeographical range of our 53 samples extends from the cold (<10°C), nutrient-rich and highly productive subarctic waters of the Iceland Basin to the warm (>25°C) oligotrophic subtropical waters off Africa. Surface water concentrations of total alkenone and alkenoates ranged from <50 ng/l in oligotrophic waters below 40°N to 2000-4500 ng/l in high latitude E. huxleyi blooms, and were well correlated with E. huxleyi cell densities, supporting the assumption that E. huxleyi is the predominant source of these compounds in the present day North Atlantic. The within-class distribution of the C37 and C38 alkenones and C36 alkenoates varied strongly as a function of temperature, and was largely unaffected by nutrient concentration, bloom status and other surface water properties. The biosynthetic response of the source organisms to growth temperature differed between the cold (<16°C) waters above 47°N and the warmer waters to the south. In cold (<16°C) waters above 47°N, the relative amounts of alkenoates and C38 alkenones synthesized was a strong function of growth temperature, while the unsaturation ratio of the alkenones (C37 and C38) was uncorrelated with temperature. Conversely, in warm (>16°C) waters below 47°N, the relative proportions of alkenoates and alkenones synthesized remained constant with increasing temperature while the unsaturation ratios of the C37 and C38 methyl alkenones (Uk37 and Uk38Me, respectively) increased linearly. The fitted regressions of Uk37 and Uk38Me versus temperature for waters >16°C were both highly significant (r**2 > 0.96) and had identical slopes (0.057) that were 50% higher than the slope (0.034) of the temperature calibration of Uk37 reported by Prahl and Wakeham (1987; doi:10.1038/330367a0) over the same temperature range. These observations suggest either a physiological adjustment in biochemical response to growth temperature above a 16-17°C threshold and/or variation between different E. huxleyi strains and/or related species inhabiting the cold and warm water regions of the eastern North Atlantic. Using our North Atlantic data set, we have produced multivariate temperature calibrations incorporating all major features of the alkenone and alkenoate data set. Predicted temperatures using multivariate calibrations are largely unbiased, with a standard error of approximately ±1°C over the entire data range. In contrast, simpler calibration models cannot adequately incorporate regional diversity and nonlinear trends with temperature. Our results indicate that calibrations based upon single variables, such as Uk37, can be strongly biased by unknown systematic errors arising from natural variability in the biosynthetic response of the source organisms to growth temperature. Multivariate temperature calibration can be expected to give more precise estimates of Integrated Production Temperatures (IPT) in the sedimentary record over a wider range of paleoenvironmental conditions, when derived using a calibration data set incorporating a similar range of natural variability in biosynthetic response.

Body mass, flipper length, oxidative balance indices and telomere length of king penguin chicks (A. patagonicus) on Possession Island

One of the reasons for animals not to grow as fast as they potentially could is that fast growth has been shown to be associated with reduced lifespan. However, we are still lacking a clear description of the reality of growth-dependent modulation of ageing mechanisms in wild animals. Using the particular growth trajectory of small king penguin chicks naturally exhibiting higher-than-normal growth rate to compensate for the winter break, we tested whether oxidative stress and telomere shortening are related to growth trajectories. Plasma antioxidant defences, oxidative damage levels and telomere length were measured at the beginning and at the end of the post-winter growth period in three groups of chicks (small chicks, which either passed away or survived the growth period, and large chicks). Small chicks that died early during the growth period had the highest level of oxidative damage and the shortest telomere lengths prior to death. Here, we show that small chicks that grew faster did it at the detriment of body maintenance mechanisms as shown by (i) higher oxidative damage and (ii) accelerated telomere loss. Our study provides the first evidence for a mechanistic link between growth and ageing rates under natural conditions.

Sea surface temperature reconstruction of ODP Site 175-1087

Successful application of the alkenone palaeothermometer, the UK'37 index, relies upon the assumption that fossil alkenone synthesisers responded to growth-temperature changes in a similar manner to the modern producers, chiefly the coccolithophores Emiliania huxleyi and Gephyrocapsa oceanica. We compare coccolith and UK'37 data from ODP Site 1087 in the south-east Atlantic between 1500 and 500 ka, and show that evolutionary events and changes in species dominance within the coccolithophore populations had little impact on the UK'37 record. The relative abundances of the C37 and C38 alkenones also closely resembled those found in modern populations, and suggest a similar temperature sensitivity of UK'37 during the early and mid-Pleistocene to that found at present. These results support the application of the UK'37 index to reconstruct sea-surface temperatures (SSTs) throughout the Quaternary. The UK'37 record at ODP Site 1087 contains an SST signal that documents the emergence of the 100-kyr cycles that characterise the late Quaternary ice volume records. This is preceded by significant cooling at ODP Site 1087, marked by a negative shift in SSTs and a positive shift in the planktonic delta18O some 250-kyr earlier, at ca 1150-1000 ka. This results in a permanent fall in average SSTs of around 1.5 °C. The predicted increase in aridity onshore as a result of this cooling can be identified in a number of published records from southern Africa, and may have played a role in some important evolutionary events of the mid-Pleistocene.

Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment

Anthropogenic CO2 is progressively acidifying the ocean, but the responses of harmful algal bloom species that produce toxins that can bioaccumulate remain virtually unknown. The neurotoxin domoic acid is produced by the globally-distributed diatom genus Pseudo-nitzschia. This toxin is responsible for amnesic shellfish poisoning, which can result in illness or death in humans and regularly causes mass mortalities of marine mammals and birds. Domoic acid production by Pseudo-nitzschia cells is known to be regulated by nutrient availability, but potential interactions with increasing seawater CO2 concentrations are poorly understood. Here we present experiments measuring domoic acid production by acclimatized cultures of Pseudo-nitzschia fraudulenta that demonstrate a strong synergism between projected future CO2 levels (765 ppm) and silicate-limited growth, which greatly increases cellular toxicity relative to growth under modern atmospheric (360 ppm) or pre-industrial (200 ppm) CO2 conditions. Cellular Si:C ratios decrease with increasing CO2, in a trend opposite to that seen for domoic acid production. The coastal California upwelling system where this species was isolated currently exhibits rapidly increasing levels of anthropogenic acidification, as well as widespread episodic silicate limitation of diatom growth. Our results suggest that the current ecosystem and human health impacts of toxic Pseudo-nitzschia blooms could be greatly exacerbated by future ocean acidification and 'carbon fertilization' of the coastal ocean.

Impact of elevated pCO2 on paralytic shellfish poisoning toxin content and composition in Alexandrium tamarense

Ocean acidification is considered a major threat to marine ecosystems and may particularly affect primary producers. Here we investigated the impact of elevated pCO2 on paralytic shellfish poisoning toxin (PST) content and composition in two strains of Alexandrium tamarense, Alex5 and Alex2. Experiments were carried out as dilute batch to keep carbonate chemistry unaltered over time. We observed only minor changes with respect to growth and elemental composition in response to elevated pCO2. For both strains, the cellular PST content, and in particular the associated cellular toxicity, was lower in the high CO2 treatments. In addition, Alex5 showed a shift in its PST composition from a nonsulfated analogue towards less toxic sulfated analogues with increasing pCO2. Transcriptomic analyses suggest that the ability of A. tamarense to maintain cellular homeostasis is predominantly regulated on the post-translational level rather than on the transcriptomic level. Furthermore, genes associated to secondary metabolite and amino acid metabolism in Alex5 were down-regulated in the high CO2 treatment, which may explain the lower PST content. Elevated pCO2 also induced up-regulation of a putative sulfotransferase sxtN homologue and a substantial down-regulation of several sulfatases. Such changes in sulfur metabolism may explain the shift in PST composition towards more sulfated analogues. All in all, our results indicate that elevated pCO2 will have minor consequences for growth and elemental composition, but may potentially reduce the cellular toxicity of A. tamarense.

Control de vibraciones en pasarelas peatonales

En los últimos años se ha construido un gran número de pasarelas peatonales como respuesta a la demanda de nuevas vías de paso en las ciudades. Estas estructuras tienen requisitos constructivos menos exigentes en comparación con otros tipos de puentes, lo cual ha facilitado el desarrollo de diseños con nuevos esquemas resistentes, complicadas geometrías y el empleo de nuevos materiales. En general estas estructuras son esbeltas, ligeras y poco amortiguadas, lo que en ocasiones ha generado problemas de vi-braciones al paso de peatones una vez puestas en servicio. Las normativas actuales son cada vez más sensibles a esta problemática, recomendando diseños cuyas frecuencias naturales deben estar alejadas de los rangos de frecuencia de paso típicos de los peatones y fijando límites de confort en forma de valores máximos de aceleración permitidos, asegurándose así un correcto comportamiento de la estructura. En el presente artículo se analiza esta problemática desde un punto de vista práctico. Para ello se muestran los puntos clave de las normativas y guías de diseño de pasarelas que se pueden encontrar actualmente en la bibliografía, se presentan las técnicas que habitualmente se emplean en el análisis dinámico experimental de estas estructuras, y se comentan las soluciones a las que generalmente se recurre para mejorar su comportamiento dinámico. Por último, se muestran los trabajos llevados a cabo por el Centro Tecnológico CARTIF en colaboración con las Universidades de Valladolid y Castilla-La Mancha en la pasarela peatonal del Museo de la Ciencia de Valladolid. Estos trabajos incluyen: (1) el estudio dinámico de los tres vanos metálicos de dicha pasarela, (2) el diseño e implementación de un amortiguador de masa sintonizado en el vano más sensible a las vibraciones, (3) la implementación de un amortiguador de masa activo utilizando un excitador electrodinámico, y (4) el desarrollo de pruebas para la verificación del estado de servicio de la pasarela. In the last years, a wide number of footbridges have been built as demand response of more direct pathways in cities. These structures have lower building requirements as compared with standard bridges. This circumstance has facilitated the development of new structural design with complex geometries and innovative materials. As a result, these structures may be slender, light and low damped, leading to vibration problems once in service. The current codes take into account this problem, and recommend designs with natural frequencies away from the typical pedestrian pacing rates and fix comfort limits to guarantee the serviceability of the structure.This paper studies this problem from a practical point of view. Thus, the key points of codes and footbridges guidelines are showed, the typical experimental dynamic analysis techniques are presented, and the usual solutions adopted to improve the dynamic performance of these structures are discussed. Finally, the works carried out on the Valladolid Science Museum Footbridge by Centro Tecnológico CARTIF in collaboration with the Universities of Valladolid and Castilla-La Mancha are showed. These works include: (1) the dynamic study of the three steel spans of the footbridge, (2) the design and implementation of a tuned mass damper in the liveliest span, (3) the implementation of an active mass damper using an electrodynamic shaker, and (4) the development of field tests to assess the serviceability of such span.

Partioning of dietary energy of chickens fed maize or wheat-based diets with and without a commercial blend of phytogenic feed additives

The aim of the study was to investigate the effects of a standardized mixture of a commercial blend of phytogenic feed additives containing 5% carvacrol, 3% cinnamaldehyde, and 2% capsicum on utilization of dietary energy and performance in broiler chickens. Four experimental diets were offered to the birds from 7 to 21 d of age. These included 2 basal control diets based on either wheat or maize that contained 215 g CP/kg and 12.13 MJ/kg ME and another 2 diets using the basal control diets supplemented with the plant extracts combination at 100 mg/kg diet. Each diet was fed to 16 individually penned birds following randomization. Dietary plant extracts improved feed intake and weight gain (P < 0.05) and slightly (P < 0.1) improved feed efficiency of birds fed the maize-based diet. Supplementary plant extracts did not change dietary ME (P > 0.05) but improved (P < 0.05) dietary NE by reducing the heat increment (P < 0.05) per kilogram feed intake. Feeding phytogenics improved (P < 0.05) total carcass energy retention and the efficiency of dietary ME for carcass energy retention. The number of interactions between type of diet and supplementary phytogenic feed additive suggest that the chemical composition and the energy to protein ratio of the diet may influence the efficiency of phytogenics when fed to chickens. The experiment showed that although supplementary phytogenic additives did not affect dietary ME, they caused a significant improvement in the utilization of dietary energy for carcass energy retention but this did not always relate to growth performance.

El estuco a fuego: técnica tradicional y posibilidades de actualización

La presente tesis analiza el resultado de tratar con una plancha caliente la superficie de un estuco de cal, en estado fresco. El estuco a fuego –nombre que recibe la técnica actualmente en desuso- fue muy valorada en un pasado relativamente reciente, de forma que actualmente aparece ciertamente mitificada y sin caracterizar, habiéndose perdido prácticamente su técnica de ejecución. En el capítulo 1 se exponen las hipótesis y objetivos de la investigación realizada, y el e interés y oportunidad de la tesis. En el capítulo 2 se analizan los antecedentes y usos históricos de los estucos, con especial dedicación a los de cal y más concretamente los “planchados” o "al fuego", que son el tema central de esta tesis. Tras un detallado análisis de la bibliografía actual y de la época, se exponen las contradicciones que sobre este acabado se deducen de los diferentes autores consultados. También se sitúa cronológicamente la técnica, en un intento siempre difícil de poner límites a un proceso continuo. Se propone finalmente una técnica de ejecución que, tomando las descripciones más coherentes y creíbles, y mejor contrastadas de los diferentes autores, recupera la técnica perdida, lo que se prueba mediante las correspondientes "ejecuciones piloto". En el capítulo 3 se expone la metodología experimental seguida para caracterizar el efecto de la aplicación de la plancha caliente sobre el estuco de cal: en primer lugar se recuperará en taller el proceso de ejecución a partir de la bibliografía revisada, tomando las descripciones más coherentes y mejor contrastadas de los diferentes autores; posteriormente, se analiza el único caso conocido de esta técnica en el que ha sido posible sacar probetas; a continuación se contrastarán ambas informaciones con objeto de validar el proceso llevado a cabo; por último se proponen los ensayos realizados a las muestras de laboratorio hechas con la técnica recuperada y comparada con el caso real. En el capítulo 4 se lleva a la práctica lo desarrollado en el capítulo anterior, principalmente para determinar los posibles cambios en la superficie acabada, así como en su absorción de agua, brillo, dureza, y porosidad superficial del estuco. Los resultados obtenidos son objeto de análisis y de comparación. Toda la información estudiada hace posible las conclusiones que aparecen el capítulo 5 y que delimitan claramente el efecto que provoca el uso de la plancha caliente sobre el acabado en varios aspectos: economía en el proceso, mejora de los efectos de imitación de mármol y aumento de las propiedades físicas solicitadas a un revestimiento continuo de estas características. Termina el capítulo proponiendo unas cuestiones como futuras líneas de desarrollo de la investigación. ABSTRACT This thesis analyzes the result of dealing with a hot iron the surface of a plaster of lime, fresh state. The stucco fire-name receiving the technique in disuse today- was highly valued in the relatively recent past, so now certainly appears mystified and uncharacterized, having almost lost his playing technique. In Chapter 1 the assumptions and objectives of the research are presented, and the interest and opportunity of the thesis. In chapter 2 the background and historical uses of stucco, with particular attention to the lime and more specifically the "pressed" or "Fire", which are the focus of this thesis are discussed. After a detailed analysis of the current literature and the time, the contradictions that this finish is derived from the different authors consulted are exposed. Also it is situated chronologically the technique, in an always difficult to set limits to a continuous attempt. A technique of execution, taking the most consistent and credible descriptions, and better contrasting of different authors, recovers lost art, which is proved by the corresponding "pilot runs" was finally proposed. In Chapter 3 the experimental methodology to characterize the effect of the application of the hot iron on the lime stucco is exposed: first workshop will recover in the implementation process from the literature reviewed, taking the most consistent descriptions and better contrasted different authors; then it is analyzed the only known case in which this technique has been possible to take samples; then both information will be compared in order to validate the process undertaken; Finally tests performed at the laboratory samples made with the technique recovered and compared with the real case are proposed. Chapter 4 takes practice what it was developed in the previous chapter, mainly to determine possible changes in the finished surface as well as water absorption, gloss, hardness, surface porosity from the stucco. The results are analyzed and compared. All information makes possible to study the conclusions that appear on Chapter 5 and clearly define the effect caused by the use of the hot iron on the finish in several aspects: economy in the process, enhancing the effects of marble imitation and increased physical properties applied to a continuous coating of these features. Chapter finalizes proposing some issues and future lines of research and development.

Límites de ductilidad y de servicio al cálculo en carga última de estructuras de hormigón armado y de fábrica

En este trabajo se aborda una cuestión central en el diseño en carga última de estructuras de hormigón armado y de fábrica: la posibilidad efectiva de que las deformaciones plásticas necesarias para verificar un estado de rotura puedan ser alcanzadas por las regiones de la estructura que deban desarrollar su capacidad última para verificar tal estado. Así, se parte de las decisiones de diseño que mediante mera estática aseguran un equilibrio de la estructura para las cargas últimas que deba resistir, pero determinando directamente el valor de las deformaciones necesarias para llegar a tal estado. Por tanto, no se acude a los teoremas de rotura sin más, sino que se formula el problema desde un punto de vista elastoplástico. Es decir, no se obvia el recorrido que la estructura deba realizar en un proceso de carga incremental monótono, de modo que las regiones no plastificadas contribuyen a coaccionar las libres deformaciones plásticas que, en la teoría de rotura, se suponen. En términos de trabajo y energía, se introduce en el balance del trabajo de las fuerzas externas y en el de la energía de deformación, aquella parte del sistema que no ha plastificado. Establecido así el balance energético como potencial del sistema es cuando la condición de estacionariedad del mismo hace determinados los campos de desplazamientos y, por tanto, el de las deformaciones plásticas también. En definitiva, se trata de un modo de verificar si la ductilidad de los diseños previstos es suficiente, y en qué medida, para verificar el estado de rotura previsto, para unas determinadas cargas impuestas. Dentro del desarrollo teórico del problema, se encuentran ciertas precisiones importantes. Entre ellas, la verificación de que el estado de rotura a que se llega de manera determinada mediante el balance energético elasto-plástico satisface las condiciones de la solución de rotura que los teoremas de carga última predicen, asegurando, por tanto, que la solución determinada -unicidad del problema elásticocoincide con el teorema de unicidad de la carga de rotura, acotando además cuál es el sistema de equilibrio y cuál es la deformada de colapso, aspectos que los teoremas de rotura no pueden asegurar, sino sólo el valor de la carga última a verificar. Otra precisión se basa en la particularidad de los casos en que el sistema presenta una superficie de rotura plana, haciendo infinitas las posibilidades de equilibrio para una misma deformada de colapso determinada, lo que está en la base de, aparentemente, poder plastificar a antojo en vigas y arcos. Desde el planteamiento anterior, se encuentra entonces que existe una condición inherente a cualquier sistema, definidas unas leyes constitutivas internas, que permite al mismo llegar al inicio del estado de rotura sin demandar deformación plástica alguna, produciéndose la plastificación simultánea de todas las regiones que hayan llegado a su solicitación de rotura. En cierto modo, se daría un colapso de apariencia frágil. En tal caso, el sistema conserva plenamente hasta el final su capacidad dúctil y tal estado actúa como representante canónico de cualquier otra solución de equilibrio que con idéntico criterio de diseño interno se prevea para tal estructura. En la medida que el diseño se acerque o aleje de la solución canónica, la demanda de ductilidad del sistema para verificar la carga última será menor o mayor. Las soluciones que se aparten en exceso de la solución canónica, no verificarán el estado de rotura previsto por falta de ductilidad: la demanda de deformación plástica de alguna región plastificada estará más allá de la capacidad de la misma, revelándose una carga de rotura por falta de ductilidad menor que la que se preveía por mero equilibrio. Para la determinación de las deformaciones plásticas de las rótulas, se ha tomado un modelo formulado mediante el Método de los Elementos de Contorno, que proporciona un campo continuo de desplazamientos -y, por ende, de deformaciones y de tensiones- incluso en presencia de fisuras en el contorno. Importante cuestión es que se formula la diferencia, nada desdeñable, de la capacidad de rotación plástica de las secciones de hormigón armado en presencia de cortante y en su ausencia. Para las rótulas de fábrica, la diferencia se establece para las condiciones de la excentricidad -asociadas al valor relativo de la compresión-, donde las diferencias entres las regiones plastificadas con esfuerzo normal relativo alto o bajo son reseñables. Por otro lado, si bien de manera un tanto secundaria, las condiciones de servicio también imponen un límite al diseño previo en carga última deseado. La plastificación lleva asociadas deformaciones considerables, sean locales como globales. Tal cosa impone que, en estado de servicio, si la plastificación de alguna región lleva asociadas fisuraciones excesivas para el ambiente del entorno, la solución sea inviable por ello. Asimismo, las deformaciones de las estructuras suponen un límite severo a las posibilidades de su diseño. Especialmente en edificación, las deformaciones activas son un factor crítico a la hora de decidirse por una u otra solución. Por tanto, al límite que se impone por razón de ductilidad, se debe añadir el que se imponga por razón de las condiciones de servicio. Del modo anterior, considerando las condiciones de ductilidad y de servicio en cada caso, se puede tasar cada decisión de diseño con la previsión de cuáles serán las consecuencias en su estado de carga última y de servicio. Es decir, conocidos los límites, podemos acotar cuáles son los diseños a priori que podrán satisfacer seguro las condiciones de ductilidad y de servicio previstas, y en qué medida. Y, en caso de no poderse satisfacer, qué correcciones debieran realizarse sobre el diseño previo para poderlas cumplir. Por último, de las consecuencias que se extraen de lo estudiado, se proponen ciertas líneas de estudio y de experimentación para poder llegar a completar o expandir de manera práctica los resultados obtenidos. ABSTRACT This work deals with a main issue for the ultimate load design in reinforced concrete and masonry structures: the actual possibility that needed yield strains to reach a ultimate state could be reached by yielded regions on the structure that should develop their ultimate capacity to fulfill such a state. Thus, some statically determined design decisions are posed as a start for prescribed ultimate loads to be counteracted, but finding out the determined value of the strains needed to reach the ultimate load state. Therefore, ultimate load theorems are not taken as they are, but a full elasto-plastic formulation point of view is used. As a result, the path the structure must develop in a monotonus increasing loading procedure is not neglected, leading to the fact that non yielded regions will restrict the supposed totally free yield strains under a pure ultimate load theory. In work and energy terms, in the overall account of external forces work and internal strain energy, those domains in the body not reaching their ultimate state are considered. Once thus established the energy balance of the system as its potential, by imposing on it the stationary condition, both displacements and yield strains appear as determined values. Consequently, what proposed is a means for verifying whether the ductility of prescribed designs is enough and the extent to which they are so, for known imposed loads. On the way for the theoretical development of the proposal, some important aspects have been found. Among these, the verification that the conditions for the ultimate state reached under the elastoplastic energy balance fulfills the conditions prescribed for the ultimate load state predicted through the ultimate load theorems, assuring, therefore, that the determinate solution -unicity of the elastic problemcoincides with the unicity ultimate load theorem, determining as well which equilibrium system and which collapse shape are linked to it, being these two last aspects unaffordable by the ultimate load theorems, that make sure only which is the value of the ultimate load leading to collapse. Another aspect is based on the particular case in which the yield surface of the system is flat -i.e. expressed under a linear expression-, turning out infinite the equilibrium possibilities for one determined collapse shape, which is the basis of, apparently, deciding at own free will the yield distribution in beams and arches. From the foresaid approach, is then found that there is an inherent condition in any system, once defined internal constitutive laws, which allows it arrive at the beginning of the ultimate state or collapse without any yield strain demand, reaching the collapse simultaneously for all regions that have come to their ultimate strength. In a certain way, it would appear to be a fragile collapse. In such a case case, the system fully keeps until the end its ductility, and such a state acts as a canonical representative of any other statically determined solution having the same internal design criteria that could be posed for the that same structure. The extent to which a design is closer to or farther from the canonical solution, the ductility demand of the system to verify the ultimate load will be higher or lower. The solutions being far in excess from the canonical solution, will not verify the ultimate state due to lack of ductility: the demand for yield strains of any yielded region will be beyond its capacity, and a shortcoming ultimate load by lack of ductility will appear, lower than the expected by mere equilibrium. For determining the yield strains of plastic hinges, a Boundary Element Method based model has been used, leading to a continuous displacement field -therefore, for strains and stresses as well- even if cracks on the boundary are present. An important aspect is that a remarkable difference is found in the rotation capacity between plastic hinges in reinforced concrete with or without shear. For masonry hinges, such difference appears when dealing with the eccentricity of axial forces -related to their relative value of compression- on the section, where differences between yield regions under high or low relative compressions are remarkable. On the other hand, although in a certain secondary manner, serviceability conditions impose limits to the previous ultimate load stated wanted too. Yield means always big strains and deformations, locally and globally. Such a thing imposes, for serviceability states, that if a yielded region is associated with too large cracking for the environmental conditions, the predicted design will be unsuitable due to this. Furthermore, displacements must be restricted under certain severe limits that restrain the possibilities for a free design. Especially in building structures, active displacements are a critical factor when chosing one or another solution. Then, to the limits due to ductility reasons, other limits dealing with serviceability conditions shoud be added. In the foresaid way, both considering ductility and serviceability conditions in every case, the results for ultimate load and serviceability to which every design decision will lead can be bounded. This means that, once the limits are known, it is possible to bound which a priori designs will fulfill for sure the prescribed ductility and serviceability conditions, and the extent to wich they will be fulfilled, And, in case they were not, which corrections must be performed in the previous design so that it will. Finally, from the consequences derived through what studied, several study and experimental fields are proposed, in order to achieve a completeness and practical expansion of the obtained results.

Temperature and precipitation effects on wheat yield across a European transect: a crop model ensemble analysis using impact response surfaces

This study explored the utility of the impact response surface (IRS) approach for investigating model ensemble crop yield responses under a large range of changes in climate. IRSs of spring and winter wheat Triticum aestivum yields were constructed from a 26-member ensemble of process-based crop simulation models for sites in Finland, Germany and Spain across a latitudinal transect. The sensitivity of modelled yield to systematic increments of changes in temperature (-2 to +9°C) and precipitation (-50 to +50%) was tested by modifying values of baseline (1981 to 2010) daily weather, with CO2 concentration fixed at 360 ppm. The IRS approach offers an effective method of portraying model behaviour under changing climate as well as advantages for analysing, comparing and presenting results from multi-model ensemble simulations. Though individual model behaviour occasionally departed markedly from the average, ensemble median responses across sites and crop varieties indicated that yields decline with higher temperatures and decreased precipitation and increase with higher precipitation. Across the uncertainty ranges defined for the IRSs, yields were more sensitive to temperature than precipitation changes at the Finnish site while sensitivities were mixed at the German and Spanish sites. Precipitation effects diminished under higher temperature changes. While the bivariate and multi-model characteristics of the analysis impose some limits to interpretation, the IRS approach nonetheless provides additional insights into sensitivities to inter-model and inter-annual variability. Taken together, these sensitivities may help to pinpoint processes such as heat stress, vernalisation or drought effects requiring refinement in future model development.

ECA1 complements yeast mutants defective in Ca2+ pumps and encodes an endoplasmic reticulum-type Ca2+-ATPase in Arabidopsis thaliana

To understand the structure, role, and regulation of individual Ca2+ pumps in plants, we have used yeast as a heterologous expression system to test the function of a gene from Arabidopsis thaliana (ECA1). ECA1 encoded a 116-kDa polypeptide that has all the conserved domains common to P-type Ca2+ pumps (EC 3.6.1.38). The amino acid sequence shared more identity with sarcoplasmic/endoplasmic reticulum (53%) than with plasma membrane (32%) Ca2+ pumps. Yeast mutants defective in a Golgi Ca2+ pump (pmr1) or both Golgi and vacuolar Ca2+ pumps (pmr1 pmc1 cnb1) were sensitive to growth on medium containing 10 mM EGTA or 3 mM Mn2+. Expression of ECA1 restored growth of either mutant on EGTA. Membranes were isolated from the pmr1 pmc1 cnb1 mutant transformed with ECA1 to determine if the ECA1 polypeptide (ECA1p) could be phosphorylated as intermediates of the reaction cycle of Ca2+-pumping ATPases. In the presence of [γ-32P]ATP, ECA1p formed a Ca2+-dependent [32P]phosphoprotein of 106 kDa that was sensitive to hydroxylamine. Cyclopiazonic acid, a blocker of animal sarcoplasmic/endoplasmic reticulum Ca2+ pumps, inhibited the formation of the phosphoprotein, whereas thapsigargin did not. Immunoblotting with an antibody against the carboxyl tail showed that ECA1p was associated mainly with the endoplasmic reticulum membranes isolated from Arabidopsis plants. The results support the model that ECA1 encodes an endoplasmic reticulum-type Ca2+ pump in Arabidopsis. The ability of ECA1p to restore growth of mutant pmr1 on medium containing Mn2+, and the formation of a Mn2+-dependent phosphoprotein suggested that ECA1p may also regulate Mn2+ homeostasis by pumping Mn2+ into endomembrane compartments of plants.

Wild-type p53 triggers a rapid senescence program in human tumor cells lacking functional p53

The p53 tumor suppressor gene has been shown to play an important role in determining cell fate. Overexpression of wild-type p53 in tumor cells has been shown to lead to growth arrest or apoptosis. Previous studies in fibroblasts have provided indirect evidence for a link between p53 and senescence. Here we show, using an inducible p53 expression system, that wild-type p53 overexpression in EJ bladder carcinoma cells, which have lost functional p53, triggers the rapid onset of G1 and G2/M growth arrest associated with p21 up-regulation and repression of mitotic cyclins (cyclin A and B) and cdc2. Growth arrest in response to p53 induction became irreversible within 48-72 h, with cells exhibiting morphological features as well as specific biochemical and ultrastructural markers of the senescent phenotype. These findings provide direct evidence that p53 overexpression can activate the rapid onset of senescence in tumor cells.

Inhibition of DNA methyltransferase stimulates the expression of signal transducer and activator of transcription 1, 2, and 3 genes in colon tumor cells

Inhibitors of DNA methyltransferase, typified by 5-aza-2′-deoxycytidine (5-Aza-CdR), induce the expression of genes transcriptionally down-regulated by de novo methylation in tumor cells. We utilized gene expression microarrays to examine the effects of 5-Aza-CdR treatment in HT29 colon adenocarcinoma cells. This analysis revealed the induction of a set of genes that implicated IFN signaling in the HT29 cellular response to 5-Aza-CdR. Subsequent investigations revealed that the induction of this gene set correlates with the induction of signal transducer and activator of transcription (STAT) 1, 2, and 3 genes and their activation by endogenous IFN-α. These observations implicate the induction of the IFN-response pathway as a major cellular response to 5-Aza-CdR and suggests that the expression of STATs 1, 2, and 3 can be regulated by DNA methylation. Consistent with STAT’s limiting cell responsiveness to IFN, we found that 5-Aza-CdR treatment sensitized HT29 cells to growth inhibition by exogenous IFN-α2a, indicating that 5-Aza-CdR should be investigated as a potentiator of IFN responsiveness in certain IFN-resistant tumors.