Implicação das Alterações Legislativas ao Regulamento de Desempenho Energético de Edifícios de Habitação nas Soluções Construtivas

A presente dissertação centra-se no estudo das implicações originadas, ao nível das soluções construtivas presentes na envolvente dos edifícios de habitação, pelas recentes alterações efetuadas ao Regulamento de Desempenho Energético de Edifícios de Habitação (REH). Com o intuito de aferir o desempenho energético, através da aplicação do REH, considerou-se como caso de estudo um edifício de habitação novo, unifamiliar com tipologia T3, localizado a cerca de 10 metros acima do nível médio das águas do mar e na periferia da zona urbana de Vila Nova de Gaia. Após o levantamento das necessidades energéticas do edifício em estudo, realizaram-se diversas simulações, com o intuito de identificar e quantificar as alterações provocadas pela entrada em vigor da Portaria 379-A/2015, de 22 de outubro. Inicialmente estudou-se o comportamento térmico da habitação unifamiliar admitindo diferentes soluções construtivas: as soluções que cumpriam com as exigências em vigor até ao final de 2015 e as que cumprem as imposições atuais. Desta forma tentou perceber-se quais as implicações dessas alterações nas necessidades energéticas da habitação. Em seguida, e utilizando o mesmo conceito da simulação inicial, fez-se um estudo considerando que a fração se situava nas diferentes zonas climáticas existentes em Portugal. Para que tal fosse possível, teve que se considerar a implantação da habitação em diferentes localizações geográficas e a diferentes altitudes. Também se procurou avaliar a importância que as pontes térmicas planas assumem nas transferências de calor, nas duas estações. Assim, foi necessário fazer um pré- dimensionamento da solução estrutural adotada, quantificar a área destes elementos e o respetivo coeficiente de transmissão. Quantificou-se, posteriormente, quais as necessidades energéticas obtidas com a solução estrutural perfeitamente definida e as que se obteriam se se desprezasse a sua existência. Com as análises comparativas dos diferentes resultados obtidos, verificou-se que as atualizações das exigências regulamentares a que os edifícios de habitação estão sujeitos originam grande impacto nos sistemas construtivos adotados.

Mir-190b negatively contributes to the Trypanosoma cruzi -infected cell survival by repressing PTEN protein expression

Chagas disease, which is caused by the intracellular protozoan Trypanosoma cruzi , is a serious health problem in Latin America. The heart is one of the major organs affected by this parasitic infection. The pathogenesis of tissue remodelling, particularly regarding cardiomyocyte behaviour after parasite infection, and the molecular mechanisms that occur immediately following parasite entry into host cells are not yet completely understood. Previous studies have reported that the establishment of parasitism is connected to the activation of the phosphatidylinositol- 3 kinase (PI3K), which controls important steps in cellular metabolism by regulating the production of the second messenger phosphatidylinositol-3,4,5-trisphosphate. Particularly, the tumour suppressor PTEN is a negative regulator of PI3K signalling. However, mechanistic details of the modulatory activity of PTEN on Chagas disease have not been elucidated. To address this question, H9c2 cells were infected with T. cruzi Berenice 62 strain and the expression of a specific set of microRNAs (miRNAs) were investigated. Our cellular model demonstrated that miRNA-190b is correlated to the decrease of cellular viability rates by negatively modulating PTEN protein expression in T. cruzi-infected cells.

Convento de S. José da Esperança - Évora: proposta de valorização histórico-arquitectónica

A presente dissertação de mestrado visa a apresentação de uma proposta de valorização para o espaço edificado do Convento de S. José da Esperança, da cidade de Évora. Procurámos traçar a história do Convento de S. José ao longo de um período de cerca de 200 anos, apresentando ainda as várias funções do imóvel, desde a sua extinção até à atualidade. Abordámos a questão da instalação física do cenóbio, relacionando-a com as razões da escolha da Ordem Carmelita Descalça. Contextualizámos este especto com as origens da Ordem do Carmo, a reforma desta Ordem, originando o nascimento da Ordem Carmelita Descalça, e a sua entrada em Portugal. Sublinhámos a importância das fundadoras e o seu legado testamentário para a subsistência desta instituição religiosa e como essa subsistência conheceu vicissitudes e contrariedades várias ao longo da vida da comunidade. Procurámos depois entender o espaço conventual edificado, pelo que procedemos a uma análise histórico-arquitetónica do conjunto conventual, nomeadamente a ampliação dos espaços, e depois da extinção e da mudança de propriedade, a adaptação a novas funções e as necessárias intervenções nele ocorridas. Por fim, atendendo à história do Convento, à riqueza patrimonial que ele representa tanto para a sociedade em geral como para os Eborenses em particular, atendendo à sua inserção no Centro Histórico de Évora - CHE, com a classificação de Património Mundial atribuída pela UNESCO em 1986, e atendendo ao vasto quadro legislativo nacional e internacional relativo à proteção e valorização do património cultural, apresentámos a nossa proposta de valorização do Conjunto Conventual, que nos parece, em conformidade com o exposto, a mais apropriada àquele espaço. ABSTRACT: This master's thesis aims to propose a recovery area for the valorization of the building space of Convento de S. José da Esperança, in Évora. We traced the history of the Convent of S. José over a period of about 200 years, by exploring the various functions of the building, since its extinction as religious community until now. We addressed the question of the physical installation the life of the religious community, linking it with the reasons for the choice of the Ordem Carmelita Descalça's to settle there. This aspect was then contextualized with the origins of the Ordem do Carmo, the reform of the Order, resulting in the birth of the Ordem Carmelita Descalça, and its entry into Portugal. We emphasized the importance of the founders and their legacy to the livelihood of the subsistence of this religious institution. Along the history the Ordem experienced several setbacks and problems over the life of the community. Our study led to an understanding of the conventual space as a physical building. For that we undertook an historical and architectonical analysis of the conventual set, namely the enlargement of spaces, and after the extinction and the property change, the adaptation to new functions and the necessary interventions that it suffered. We presented our proposal of historical and architectonical valorization of the convent, taking into account the history of the Convent, as a rich heritage, not only for the city of Évora, but also to the country, as a whole. We took into account the fact that the convent is inside the historical centre of Évora (World Heritage City- UNESCO- since 1986), and it must comply to national and international legislative framework for the protection and enhancement of cultural heritage. Our proposal of recovery of the conventual set is, in line with the above, the most appropriate to that space.

Forças Armadas de Cabo Verde : O impacto económico e financeiro da profissionalização das Forças Armadas

O presente Trabalho de Investigação Aplicada intitulado “Forças Armadas de Cabo Verde. O impacto Económico e Financeiro da profissionalização das Forças Armadas”, surge no âmbito dos cursos ministrados na Academia Militar, e tem como objetivo principal estudar as Forças Armadas de Cabo Verde e identificar os impactos que a adoção de um modelo profissional pode ter no orçamento da Defesa. Este trabalho contém uma componente teórica que serve de sustentação à investigação, em que aborda aspetos importantes relativos à profissionalização das Forças Armadas, faz uma abordagem às Forças Armadas de Cabo Verde com base em legislação afeta às mesmas para se perceber quais os eventuais impactos que podem advir da adoção do modelo profissional, e as suas implicações no orçamento da Defesa Nacional. A segunda componente comporta uma pesquisa e recolha de dados e informação através de entrevistas que permitiram obter as conclusões e responder ao problema de estudo. Da análise teórica e dos dados recolhidos verifica-se que a adoção de um modelo profissional nas Forças Armadas de Cabo Verde implica uma alteração nos processos de recrutamento atualmente existente, estabelecendo requisitos para a entrada na Instituição Militar, tendo em conta as necessidades da mesma sendo que, a adoção deste modelo também tem implicações nos salários. Assim, concluímos que a adoção deste modelo implica uma alteração significativa no orçamento da Defesa, resultante do aumento dos gastos com o pessoal militar das Forças Armadas, e do maior investimento que deverá ser feito em tecnologias, na formação e treino dos quadros, pelo que o Estado deve encontrar formas para suportar tais custos. Também verifica-se que a adoção deste modelo permite uma redução da taxa de desemprego na sociedade, aumenta o poder de compra dos militares, contribuindo assim para a dinamização da economia, fornecendo ainda um clima de segurança favorecendo os investimentos, sendo por isso considerado uma mais-valia.

Analysis of contributions of herpes simplex virus type 1 UL43 protein to induction of cell-cell fusion

Purpose: To investigate whether UL43 protein, which is highly conserved in alpha- and gamma herpes viruses, and a non-glycosylated transmembrane protein, is involved in virus entry and virus-induced cell fusion. Methods: Mutagenesis was accomplished by a markerless two-step Red recombination mutagenesis system implemented on the Herpes simplex virus 1 (HSV-1) bacterial artificial chromosome (BAC). Growth properties of HSV-1 UL43 mutants were analyzed using plaque morphology and one-step growth kinetics. SDS-PAGE and Western blot was employed to assay the synthesis of the viral glycoproteins. Virus-penetration was assayed to determine if UL43 protein is required for efficient virus entry. Results: Lack of UL43 expression resulted in significantly reduced plaque sizes of syncytial mutant viruses and inhibited cell fusion induced by gBΔ28 or gKsyn20 (p < 0.05). Deletion of UL43 did not affect overall expression levels of viral glycoproteins gB, gC, gD, and gH on HSV-1(F) BAC infected cell surfaces. Moreover, mutant viruses lacking UL43 gene exhibited slower kinetics of entry into Vero cells than the parental HSV-1(F) BAC. Conclusion: Thus, these results suggest an important role for UL43 protein in mediating virus-induced membrane fusion and efficient entry of virion into target cells.

Ação coletiva de agroextrativistas em circuitos curtos de comercialização de produtos do cerrado : estudo de caso em Pirenópolis – GO

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, Programa de Pós-Graduação em Agronegócios, 2016.

Essays on internationalization in the context of heterogeneous firms

The significant increase in global trade flows in last decades has been one of the main features of the globalization process that started in the 1950s. In general, the main factors behind this increase were linked to (i) the significant reductions of trade costs and technical barriers; (ii) the improvements in transport infrastructure and telecommunications; (iii) the progress of the international financial system and the increasing legal certainty; and (iv) the development of a corporate culture that promotes the internationalization of firms as a strategic tool in order to survive and to grow. The remarkable increase of trade openness has also been observed in the Spanish economy. In this regard, it is clear that the entry into force of the Treaty of Accession of Spain to the European Economic Community (now the European Union) in 1986 played a main role in this dramatic increase. In addition, and because of the deep depression of domestic demand caused by the global financial and economic crisis that started in 2008, the external trade has become a key driver in the economic recovery of the Spanish economy...

Internationalization process of TUBOFURO

The central objective of this case study was to formulate the strategy of internationalization of Tubofuro®, discriminating relevant points from its design to its implementation. This is a company located in Leiria, Ortigosa parish, which operates, among others, in the Portuguese PVC pipes industry for which currently the domestic market is clearly insufficient, given the oversupply compared to demand. Being Tubofuro® an exporting company since 2004, the work here developed specifically intended to increase sales to the foreign market, with this representing 45% of total company's business in 2018 increasing of the number of markets through new partners to enable the positioning of Tubofuro® among the main players in each market, particularly in South American markets, North African and European. To achieve the above objectives presented a case study was applied, centred on Tubofuro® company, target of the internationalization strategy. The search carried out for the formulation of the strategy has been supported on a thorough analysis of the external environment and internal characteristics of the company, for which were crossed different types of data, quantitative, qualitative, secondary data and primary data. From this work resulted the development of internationalization and international marketing plan for the next three years, whose objectives are based on entrance and consequent growth in new markets, including the market Chilean, Peruvian, Mexican, Argentine, Algerian and German, as well growth in the presence and turnover in the markets for which Tubofuro® already exports regularly, for example Spain, France, Tunisia and Morocco. Based on the production capacity of Tubofuro® company, which will not suffer any kind of investment for incrementing but only to update, it is expected that the appropriate response capacity for the company is 8 regular markets, and could eventually arise sporadic exports to other markets not interfering with the normal production capacity of the company. The suggestion of the presented markets resulted from the study of the final price based on the one that local customers purchase a product equal or similar to Tubofuro® and the number of potential existing customers in each market. The internationalization model known as Uppsala Model corresponds to the strategy adopted by the company to its internationalization process, taking into account the philosophy of senior management and the risk aversion of them. The sales team Tubofuro® demand for each market, export a full container registering customer feedback, including quality and flow capacity in the market in order to seek a partnership agreement with a local distributor, which allows the Tubofuro® go to step two above mentioned model. The partnership agreement is based on mutual commitment to technical cooperation and trade between the Tubofuro® and partner, in order to increase the performance capacity among local customers. Only if the market presents a greater demand to our supply capacity and be justified by cost / benefit ratio, the entry into this market through a joint venture or subsidiary is that the decision will be taken. Although this is a case study, which means that is adjusted to the concrete case Tubofuro® preventing generalization of findings, we believe that this work can be a useful example for other companies in the internationalization process or the methodology adopted in formulating strategy or the outputs and conclusions drawn.

Cold acclimation of chill coma mechanisms in the locust CNS

Temperature has profound effects on the neural function and behaviour of insects. When exposed to low temperature, migratory locusts (Locusta migratoria) enter chill coma (neuromuscular paralysis) and can resume normal body functions after returning to normal temperature. Our laboratory has studied phenomena underlying environmental stress-induced comas in locusts and found that they are associated with a sudden loss of K+ homeostasis and also a temporary electrical silence in the central nervous system (CNS). However, the mechanisms underlying chill coma entry and recovery are not well understood, particularly the role of the CNS has not been determined. Here, I investigated neural function during chill coma in the locust by measuring electrical activity in the CNS. As pre-exposure to moderately low temperatures, either chronically (cold acclimation) or acutely (rapid cold hardening; RCH), has been found to improve the insect’s cold tolerance, I also determined cold acclimation and RCH protocols that will improve the locust's cold tolerance and whether these protocols affect neural shutdown during chill coma in the locust. With an implanted thermocouple in the thorax, I determined the temperature associated with a loss of responsiveness (CTmin) in intact male adult locusts. In parallel experiments, I recorded field potential (FP) in the metathoracic ganglion (MTG) in semi-intact preparations to determine the temperature that would induce neural shutdown. I found that acclimation at 10 ˚C and RCH at 4 ˚C reduced chill coma recovery time (CCRT) in intact animal preparations and RCH at 4 ˚C for 4 hours reduced the temperature at neural shutdown in semi-intact preparations. These results suggest that pre-exposure to cold can improve the locust's resistance to chill coma and support the notion that the CNS has a role in determining entry into and exit from chill coma in locusts.

WIPO and the Reinforcement of the Nagoya Protocol: Towards Effective Implementation of an Access and Benefit Sharing Regime for the Protection of Traditional Knowledge Associated with Genetic Resources

Traditional knowledge associated with genetic resources (TKaGRs) is acknowledged as a valuable resource. Its value draws from economic, social, cultural, and innovative uses. This value places TK at the heart of competing interests as between indigenous peoples who hold it and depend on it for their survival, and profitable industries which seek to exploit it in the global market space. The latter group seek, inter alia, to advance and maintain their global competitiveness by exploiting TKaGRs leads in their research and development activities connected with modern innovation. Biopiracy remains an issue of central concern to the developing world and has emerged in this context as a label for the inequity arising from the misappropriation of TKaGRs located in the South by commercial interests usually located in the North. Significant attention and resources are being channeled at global efforts to design and implement effective protection mechanisms for TKaGRs against the incidence of biopiracy. The emergence and recent entry into force of the Nagoya Protocol offers the latest example of a concluded multilateral effort in this regard. The Nagoya Protocol, adopted on the platform of the Convention on Biological Diversity (CBD), establishes an open-ended international access and benefit sharing (ABS) regime which is comprised of the Protocol as well as several complementary instruments. By focusing on the trans-regime nature of biopiracy, this thesis argues that the intellectual property (IP) system forms a central part of the problem of biopiracy, and so too to the very efforts to implement solutions, including through the Nagoya Protocol. The ongoing related work within the World Intellectual Property Organization (WIPO), aimed at developing an international instrument (or a series of instruments) to address the effective protection of TK, constitutes an essential complementary process to the Nagoya Protocol, and, as such, forms a fundamental element within the Nagoya Protocol’s evolving ABS regime-complex. By adopting a third world approach to international law, this thesis draws central significance from its reconceptualization of biopiracy as a trans-regime concept. By construing the instrument(s) being negotiated within WIPO as forming a central component part of the Nagoya Protocol, this dissertation’s analysis highlights the importance of third world efforts to secure an IP-based reinforcement to the Protocol for the effective eradication of biopiracy.

The official roster of South Carolina soldiers, sailors and marines in the World War, 1917-18, volume 1, part 1

This is the Official Roster of the South Carolina soldiers, sailors and marines who served in the World War and contains the names and records, briefly compiled, of the men and women from this state who responded to the call of the President when the United States entered the great conflict. This record gives in as compact form as practicable the following : name; serial number; residence; branch of service; date and place of entry into service; place of birth and age; organizations served in, with dates of assignments and transfers; grades with dates of appointment; engagements, if any; wound received in action, with degree and date; if killed in action or died while in service; person notified, relationship and address; oversea service; date of discharge; citations.

The official roster of South Carolina soldiers, sailors and marines in the World War, 1917-18, volume 1, part 2

This is the Official Roster of the South Carolina soldiers, sailors and marines who served in the World War and contains the names and records, briefly compiled, of the men and women from this state who responded to the call of the President when the United States entered the great conflict. This record gives in as compact form as practicable the following : name; serial number; residence; branch of service; date and place of entry into service; place of birth and age; organizations served in, with dates of assignments and transfers; grades with dates of appointment; engagements, if any; wound received in action, with degree and date; if killed in action or died while in service; person notified, relationship and address; oversea service; date of discharge; citations.

The official roster of South Carolina soldiers, sailors and marines in the World War, 1917-18, volume 1, part 3

This is the Official Roster of the South Carolina soldiers, sailors and marines who served in the World War and contains the names and records, briefly compiled, of the men and women from this state who responded to the call of the President when the United States entered the great conflict. This record gives in as compact form as practicable the following : name; serial number; residence; branch of service; date and place of entry into service; place of birth and age; organizations served in, with dates of assignments and transfers; grades with dates of appointment; engagements, if any; wound received in action, with degree and date; if killed in action or died while in service; person notified, relationship and address; oversea service; date of discharge; citations.

The official roster of South Carolina soldiers, sailors and marines in the World War, 1917-18, volume 2, part 1

This is the Official Roster of the South Carolina soldiers, sailors and marines who served in the World War and contains the names and records, briefly compiled, of the men and women from this state who responded to the call of the President when the United States entered the great conflict. This record gives in as compact form as practicable the following : name; serial number; residence; branch of service; date and place of entry into service; place of birth and age; organizations served in, with dates of assignments and transfers; grades with dates of appointment; engagements, if any; wound received in action, with degree and date; if killed in action or died while in service; person notified, relationship and address; oversea service; date of discharge; citations.

The official roster of South Carolina soldiers, sailors and marines in the World War, 1917-18, volume 2, part 2

This is the Official Roster of the South Carolina soldiers, sailors and marines who served in the World War and contains the names and records, briefly compiled, of the men and women from this state who responded to the call of the President when the United States entered the great conflict. This record gives in as compact form as practicable the following : name; serial number; residence; branch of service; date and place of entry into service; place of birth and age; organizations served in, with dates of assignments and transfers; grades with dates of appointment; engagements, if any; wound received in action, with degree and date; if killed in action or died while in service; person notified, relationship and address; oversea service; date of discharge; citations.