Spoligotypes of Mycobacterium tuberculosis complex isolates from patients residents of 11 states of Brazil

One of the high tuberculosis (TB) incidence countries in the world, Brazil is characterized by considerable differences in TB incidence on regional and state level. In the present study, we describe Brazilian spoligotypes of 1991 Mycobacterium tuberculosis complex (MTC) clinical isolates from patients residents of 11 states from different regions of the country, diagnosed between 1996 and 2005. By performing spoligotyping on a large number of M. tuberculosis clinical isolates, one of the main objectives of this study was to determine the major genotype families causing TB in Brazil and to verify the region-associated genotype distribution. We observed a total of 577 distinct spoligopatterns, 12.6% of these corresponded to orphan patterns while 87.4% belonged to 326 shared-types (SITs). Among the latter, 86 SITs (isolated from 178 patients) had been observed for the first time in this study, the most frequent being SIT2517 which belonged to the T3-ETH lineage and was exclusively found among patients residents of Belem, the capital of the state of Para (n = 8 isolates). Irrespective of shared-type labeling, a total of 19.5% strains were unique (unclustered) in our study as opposed to 80.5% clustered isolates (189 clusters, size range from 2 to 205 isolates). The three largest clusters were SIT42 of the Latin-America & Mediterranean (LAM) 9 clade (10.3%), SIT53 of the T clade (7.6%), and SIT50 of the Haarlem clade (5.4%). The predominant MTC lineages in Brazil in decreasing order belonged to the LAM (46%); the ill-defined T (18.6%); the Haarlem (12.2%), the X (4.7%), the S (1.9%), and the East African Indian (EAI) (0.85%) families. The rest of clades grouped together as Mycobacterium africanum, Mycobacterium bovis, Beijing, Central Asian (CAS), and the Manu types, represented less than 1% of the strains. Finally, about 15% of the isolates showed spoligotype signatures that were not yet classified among well-defined lineages. In conclusion, we provide hereby a first insight into the population structure of MTC isolates in Brazil, showing the predominance of both LAM and T family and the existence of region-associated genotypes. (C) 2011 Elsevier B.V. All rights reserved.

The United States, Central America, Dominican Republic free trade agreement (CAFTA)

Includes bibliography

United States-Latin America and the Caribbean trade developments, 2002

This document provides an overview of the most relevant developments in United States trade policy relating to Latin America and the Caribbean in 2002. U.S. policy continued to promote trade liberalization through advancing negotiations on multiple fronts- globally (WTO), regionally (FTAA) and bilaterally or sub regionally- with a view that the various negotiations are mutually reinforcing and seek to create a constructive competition for liberalization" among trade partners. The passage of Trade Promotion Authority (TPA) included in the Trade Act of August 2002 enhanced the U.S. Administration's ability to negotiate trade agreements. It provided an impetus to conclude bilateral negotiations with Chile as well as to advance a number of trade agreements currently under negotiation, including negotiations toward the Free Trade Area of the Americas (FTAA) and bilateral negotiations with Central America. The Trade Act also renewed the Generalized System of Preferences, extended the Caribbean Trade Partnership Act by liberalizing apparel provisions and augmented the Andean Trade Preference Act, increasing the list of duty free products. On the multilateral front, in partial fulfillment of the Doha mandate, the U.S. tabled in 2002 two comprehensive proposals for the reduction of trade barriers on agricultural and non-agricultural goods. Along with these trade liberalizing proposals, the U.S. Administration imposed temporary safeguard measures on key steel products to provide relief to the sectors of the steel industry that have been most affected by import surges. In addition, the U.S. Congress passed the 2002 Farm Security and Rural Investment Act that substantially increased U.S. domestic farm subsidies to shield domestic farm producers from competition from subsidized products from abroad."

United States-Latin America and the Caribbean: trade developments 2003

Includes bibliography

Penetration of the political institutions of Guatemala by the international Communist movement: threat to the peace and security of America and to the sovereignty and political independence of Guatemala.

Mode of access: Internet.

Gongora in the library of the Hispanic Society of America.

Bibliographic descriptions with location of other copies and references.

The United States in today's world: report to the National Committee of the Communist Party, U.S.A.

Mode of access: Internet.

In re mediation proceedings between division 268 of the Amalgamated Association of Street and Electric Railway Employes of America and the Cleveland Railway Company. Statement of the Cleveland Railway Company. June, 1918. Squire, Sanders & Dempsey, general counsel.

Mode of access: Internet.

The gallant deeds of our naval heroes : told for boys and girls : including the adventures of Captain Paul Jones, Commodore Perry, commodore Decatur, Admiral Farragut, Admiral Dewey, and many others of America's brave sailors who won glory in battles at home and abroad; also the story of the ships made famous by great battles and great captains /

Mode of access: Internet.

Law for the clergy [electronic resource] : a compilation of the statutes of the states of Illinois, Indiana, Iowa, Michigan, Minnesota, Ohio, and Wisconsin, relating to the duties of clergymen in the solemnization of marriage, the organization of churches and religious societies, and the protection of religious meetings and assemblies, with notes and practical forms, embracing a collation of the common law of marriage. /

Electronic text and image data.

Report to the President by the Emergency Board created June 23, 1948 by Executive order 9971 pursuant to Section 10 of the Railway Labor Act : to investigate and report upon a dispute between the Grand Trunk Western Railroad Co., Chesapeake and Ohio Railway Co., Wabash Railroad and the Ann Arbor Railroad Co. and certain of its employees represented by the National Maritime Union of America, N.M.B. cases A-2801, A-2802, A-2803, A-2804, Detroit, Michigan, July 20, 1948.

Signed: Robert G. Simmons, chairman, Thomas F. Gallagher, member, Joseph L. Miller, member.