Material Evaluation of an Elastomer, Epoxy and Lightweight Concrete Rail Attachment System for Direct Fixation Light Rail Applications

Thesis (Master's)--University of Washington, 2016-08

A fast parallel hyperspectral coded aperture algorithm for compressive sensing using OpenCL

In this paper, we develop a fast implementation of an hyperspectral coded aperture (HYCA) algorithm on different platforms using OpenCL, an open standard for parallel programing on heterogeneous systems, which includes a wide variety of devices, from dense multicore systems from major manufactures such as Intel or ARM to new accelerators such as graphics processing units (GPUs), field programmable gate arrays (FPGAs), the Intel Xeon Phi and other custom devices. Our proposed implementation of HYCA significantly reduces its computational cost. Our experiments have been conducted using simulated data and reveal considerable acceleration factors. This kind of implementations with the same descriptive language on different architectures are very important in order to really calibrate the possibility of using heterogeneous platforms for efficient hyperspectral imaging processing in real remote sensing missions.

CHARACTERIZATION OF TOBACCO MOSAIC VIRUS DIRECTED REPROGRAMMING OF PHLOEM GENE EXPRESSION TO PROMOTE VIRAL PHLOEM LOADING AND SYSTMEIC MOVEMENT

Vascular phloem loading has long been recognized as an essential step in the establishment of a systemic virus infection. Yet little is known about this process and the mechanisms that control it. In this study, an interaction between the replication protein of Tobacco mosaic virus (TMV) and phloem specific auxin/indole acetic acid (Aux/IAA) transcriptional regulators was found to modulate virus phloem loading. Promoter expression studies show TMV 126/183 kDa interacting Aux/IAAs predominantly express and accumulate within the nuclei of phloem companion cells (CC). Furthermore, CC Aux/IAA nuclear localization is disrupted upon infection with an interacting virus but not during infection with a non-interacting virus. In situ analysis of virus spread shows the inability of TMV variants to disrupt Aux/IAA CC nuclear localization correlates with a reduced ability to load into the vascular tissue. Subsequent systemic movement assays also demonstrate that a virus capable of disrupting Aux/IAA localization is significantly more competitive at systemic movement than a non-interacting virus. Similarly, CC expression and over-accumulation of a degradation-resistant-interacting Aux/IAA protein was found to selectively inhibit TMV accumulation and phloem loading. Transcriptional expression studies demonstrate a role for interacting Aux/IAA proteins in the regulation of salicylic acid and jasmonic acid dependent host defense responses as well as virus specific movement factors including pectin methylesterase that are involved in regulating plasmodesmata size exclusion limits and promoting virus cell-to-cell movement. Further characterization of the phloem environment was done using two phloem specific promoters (pSUC2 and pSULTR2;2) to generate epitope-tagged polysomal-RNA complexes. Immuno-purification using the epitope tag allowed us to obtain mRNAs bound to polysomes (the translatome) specifically in phloem tissue. We found the phloem translatome is uniquely altered during TMV infection with 90% and 88% of genes down regulated in the pSUC2 and pSULTR2;2 phloem translatomes, compared to 31% of genes down regulated in the whole plant p35S translatome. Transcripts down regulated in phloem include genes involved in callose deposition at plasmodesmata, host defense responses, and RNA silencing. Combined, these findings indicate TMV reprograms gene expression within the vascular phloem as a means to enhance phloem loading and systemic spread.

Analyse des Ermüdungsverhaltens von Beton anhand der Dehnungsentwicklung

Die Weiterentwicklungen in der Betontechnologie führten in den letzten Jahrzehnten zu Hochleistungsbetonen mit immer höheren Festigkeiten. Der Ermüdungsnachweis wurde jedoch kaum weiterentwickelt und beinhaltet immer noch sehr grobe Herangehensweisen bei der Berücksichtigung des Materialwiderstands von Beton. Für eine grundlegende Weiterentwicklung dieses Nachweises fehlt noch das notwendige Wissen zu den Mechanismen der Betonermüdung. Das Ziel dieser Arbeit war es daher, grundlegende Erkenntnisse zum Ermüdungsverhalten hochfester Betone bei unterschiedlichen zyklischen Beanspruchungen zu ermitteln und hierdurch zu einem besseren Verständnis der Mechanismen der Betonermüdung beizutragen. In der vorliegenden Arbeit wurde das Ermüdungsverhalten eines hochfesten Betons bei Druckschwellbeanspruchung anhand der Dehnungs- und Steifigkeitsentwicklungen untersucht. Betrachtet wurden dabei die Einflüsse der bezogenen Oberspannung, der Belastungsfrequenz und der Wellenform. Zusätzlich wurden, ausgehend von in der Literatur dokumentierten Ansätzen, Versuche bei monoton steigender Beanspruchung und Dauerstandbeanspruchung vergleichend durchgeführt. Die Dehnungs- und Steifigkeitsentwicklungen werden durch die untersuchten Belastungsparameter der Ermüdungsbeanspruchung eindeutig beeinflusst. Charakteristische Zusammenhänge zwischen der Beeinflussung einzelner Kenngrößen der Dehnungs- und Steifigkeitsentwicklung und der Beeinflussung der Bruchlastwechselzahlen wurden aufgezeigt. Anhand der Dehnungen und Steifigkeiten an den Phasenübergängen konnten Hinweise auf beanspru-chungsartabhängige Gefügezustände abgeleitet werden. Die vergleichende Auswertung des Dehnungsverhaltens bei monoton steigender Beanspruchung, Ermüdungsbeanspruchung und Dauerstandbeanspruchung zeigte, dass das Ermüdungsverhalten von Beton nicht adäquat in Anlehnung an andere Beanspruchungsarten beschrieben werden kann. Die Untersuchungsergebnisse wurden in eine Modellvorstellung übertragen, die zur Beurteilung der baustofflichen Phänomene bei zyklischen Beanspruchungen geeignet ist. Dabei wurde die Hypothese aufgestellt, dass sich unterschiedlich ausgeprägte Kleinst-Gefügeveränderungen beanspruchungsabhängig einstellen, die die Entstehung und Ausbreitung von Mikrorissen beeinflussen. Die detaillierte Untersuchung der Dehnungs- und Steifigkeitsentwicklungen führte zu neuen und tiefergehenden Erkenntnissen und sollte ergänzt durch die Betrachtungen von Gefügezuständen zukünftig weiterverfolgt werden.

Nutrient loading on free range layer farms

A considerable proportion of the dietary nutrients consumed by poultry are excreted in the manure. This becomes an important issue on free range farms, if manure and/or nutrients are not removed periodically from the range areas. The nutrients and trace elements in manure can accumulate in the soil and become toxic to vegetation, while also causing pollution of ground and surface water through leaching. Soil samples were collected from fourteen free range layer farms both on the range and control areas (with no exposure to poultry) to investigate comparative soil nutrient concentrations. Nutrient concentrations were also compared between fixed and rotational ranges and between farms having different bird densities. At each site, soil was collected from 10 sampling points, arranged diagonally in a grid across both the range and control areas. A sampling probe was used to collect soil from the top 10 cm depth. These were submitted for a standardised lab analysis (Apal Agricultural Laboratory, SA, Australia). Data was subjected to analysis of variance and means considered significant at P < 0.05.

Helicase binding to DnaI exposes a cryptic DNA-binding site during helicase loading in Bacillus subtilis

The Bacillus subtilis DnaI, DnaB and DnaD proteins load the replicative ring helicase DnaC onto DNA during priming of DNA replication. Here we show that DnaI consists of a C-terminal domain (Cd) with ATPase and DNA-binding activities and an N-terminal domain (Nd) that interacts with the replicative ring helicase. A Zn2+-binding module mediates the interaction with the helicase and C67, C70 and H84 are involved in the coordination of the Zn2+. DnaI binds ATP and exhibits ATPase activity that is not stimulated by ssDNA, because the DNA-binding site on Cd is masked by Nd. The ATPase activity resides on the Cd domain and when detached from the Nd domain, it becomes sensitive to stimulation by ssDNA because its cryptic DNA-binding site is exposed. Therefore, Nd acts as a molecular 'switch' regulating access to the ssDNA binding site on Cd, in response to binding of the helicase. DnaI is sufficient to load the replicative helicase from a complex with six DnaI molecules, so there is no requirement for a dual helicase loader system.

COMPRESSIVE QUANTIZATION FOR SCALABLE CLOUD RADIO ACCESS NETWORKS

With the proliferation of new mobile devices and applications, the demand for ubiquitous wireless services has increased dramatically in recent years. The explosive growth in the wireless traffic requires the wireless networks to be scalable so that they can be efficiently extended to meet the wireless communication demands. In a wireless network, the interference power typically grows with the number of devices without necessary coordination among them. On the other hand, large scale coordination is always difficult due to the low-bandwidth and high-latency interfaces between access points (APs) in traditional wireless networks. To address this challenge, cloud radio access network (C-RAN) has been proposed, where a pool of base band units (BBUs) are connected to the distributed remote radio heads (RRHs) via high bandwidth and low latency links (i.e., the front-haul) and are responsible for all the baseband processing. But the insufficient front-haul link capacity may limit the scale of C-RAN and prevent it from fully utilizing the benefits made possible by the centralized baseband processing. As a result, the front-haul link capacity becomes a bottleneck in the scalability of C-RAN. In this dissertation, we explore the scalable C-RAN in the effort of tackling this challenge. In the first aspect of this dissertation, we investigate the scalability issues in the existing wireless networks and propose a novel time-reversal (TR) based scalable wireless network in which the interference power is naturally mitigated by the focusing effects of TR communications without coordination among APs or terminal devices (TDs). Due to this nice feature, it is shown that the system can be easily extended to serve more TDs. Motivated by the nice properties of TR communications in providing scalable wireless networking solutions, in the second aspect of this dissertation, we apply the TR based communications to the C-RAN and discover the TR tunneling effects which alleviate the traffic load in the front-haul links caused by the increment of TDs. We further design waveforming schemes to optimize the downlink and uplink transmissions in the TR based C-RAN, which are shown to improve the downlink and uplink transmission accuracies. Consequently, the traffic load in the front-haul links is further alleviated by the reducing re-transmissions caused by transmission errors. Moreover, inspired by the TR-based C-RAN, we propose the compressive quantization scheme which applies to the uplink of multi-antenna C-RAN so that more antennas can be utilized with the limited front-haul capacity, which provide rich spatial diversity such that the massive TDs can be served more efficiently.

Nested Sampling and its Applications in Stable Compressive Covariance Estimation and Phase Retrieval with Near-Minimal Measurements

Compressed covariance sensing using quadratic samplers is gaining increasing interest in recent literature. Covariance matrix often plays the role of a sufficient statistic in many signal and information processing tasks. However, owing to the large dimension of the data, it may become necessary to obtain a compressed sketch of the high dimensional covariance matrix to reduce the associated storage and communication costs. Nested sampling has been proposed in the past as an efficient sub-Nyquist sampling strategy that enables perfect reconstruction of the autocorrelation sequence of Wide-Sense Stationary (WSS) signals, as though it was sampled at the Nyquist rate. The key idea behind nested sampling is to exploit properties of the difference set that naturally arises in quadratic measurement model associated with covariance compression. In this thesis, we will focus on developing novel versions of nested sampling for low rank Toeplitz covariance estimation, and phase retrieval, where the latter problem finds many applications in high resolution optical imaging, X-ray crystallography and molecular imaging. The problem of low rank compressive Toeplitz covariance estimation is first shown to be fundamentally related to that of line spectrum recovery. In absence if noise, this connection can be exploited to develop a particular kind of sampler called the Generalized Nested Sampler (GNS), that can achieve optimal compression rates. In presence of bounded noise, we develop a regularization-free algorithm that provably leads to stable recovery of the high dimensional Toeplitz matrix from its order-wise minimal sketch acquired using a GNS. Contrary to existing TV-norm and nuclear norm based reconstruction algorithms, our technique does not use any tuning parameters, which can be of great practical value. The idea of nested sampling idea also finds a surprising use in the problem of phase retrieval, which has been of great interest in recent times for its convex formulation via PhaseLift, By using another modified version of nested sampling, namely the Partial Nested Fourier Sampler (PNFS), we show that with probability one, it is possible to achieve a certain conjectured lower bound on the necessary measurement size. Moreover, for sparse data, an l1 minimization based algorithm is proposed that can lead to stable phase retrieval using order-wise minimal number of measurements.