Expressão gênica diferencial de laranja Pêra Rio (Citrus sinensis (L.) Osbeck) e Lima Ácida 'Galego' (Citrus aurantifolia Swingle) em resposta à infecção por Xanthomonas citri subsp. citri

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Parâmetros fisiológicos de laranjeiras com Huanglongbing

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms

The aim of this study was to evaluate the effects of Citrus limonum and Citrus aurantium essential oils (EOs) compared to 0.2% chlorhexidine (CHX) and 1% sodium hypochlorite (NaOCl) on multi-species biofilms formed by Candida albicans, Enterococcus faecalis and Escherichia coli. The biofilms were grown in acrylic disks immersed in broth, inoculated with microbial suspension (106 cells/mL) and incubated at 37°C / 48 h. After the biofilms were formed, they were exposed for 5 minutes to the solutions (n = 10): C. aurantium EO, C. limonum EO, 0.2% CHX, 1% NaOCl or sterile saline solution [0.9% sodium chloride (NaCl)]. Next, the discs were placed in sterile 0.9% NaCl and sonicated to disperse the biofilms. Tenfold serial dilutions were performed and the aliquots were seeded onto selective agar and incubated at 37°C / 48 h. Next, the number of colony-forming units per milliliter was counted and analyzed statistically (Tukey test, p ≤ 0.05). C. aurantium EO and NaOCl inhibited the growth of all microorganisms in multi-species biofilms. C. limonum EO promoted a 100% reduction of C. albicans and E. coli, and 49.3% of E. faecalis. CHX was less effective against C. albicans and E. coli, yielding a reduction of 68.8% and 86.7%, respectively. However, the reduction of E. faecalis using CHX (81.7%) was greater than that obtained using C. limonum EO. Both Citrus limonum and Citrus aurantium EOs are effective in controlling multi-species biofilms; the microbial reductions achieved by EOs were not only similar to those of NaOCl, but even higher than those achieved by CHX, in some cases.

Controle de doenças fúngicas e de danos por frio em pós-colheita de lima ácida Tahiti

The Tahiti lime appears very susceptible to attack by post-harvest diseases, primarily by the fungi Penicillium and Phomopsis, and also because of its high sensitivity to storage at low temperatures. In order to reduce such damage, the present study aimed to verify the efficiency of heat treatment and disinfection of pathogens in the prevention of post-harvest chilling injury of this cultivar and to compare this treatment with other products using the conventional fungicides. The heat treatments were studied with hot-water temperatures ranging between 48 and 56° C. Water at room temperature was used as a control treatment. After treatment, the fruits were kept under cold temperature at 10° C and RH 90% for about 45 days. For comparison, three other treatments were carried out simultaneously, one using imazalil, one with baking soda, and a third with sodium carbonate, these three products being applied by baths in cold water. Two groups of fruit were evaluated, one treated by immersion considering pathogens coming from the field and another by inoculation with spores of the previously isolated pathogens. For the evaluation of physical and chemical parameters of fruits, determinations were made of the skin color, texture, weight loss, size, juice yield, soluble solids, total acidity and vitamin C content. The determination of the sensitivity of the fruit to cold was made by their exposure at temperatures inducing cold damage. The design was a randomized block design with nine treatments, analyzed by the Statgraphics statistical package. Heat treatments, especially at 52° C, were shown to be more promising in the control of pathogenic fungi and cold damage, surpassing the conventional fungicides. No changes were found in the intrinsic and extrinsic parameters in relation to the application of the different treatments.

Geostatistics and remote sensing methods in the classification of images of areas cultivated with citrus

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da deriva de clomazone, aplicado isoladamente ou em mistura com ametryn, sobre características produtivas de laranjeira 'Hamlin'

This experiment aimed to evaluate the effect of increasing clomazone (sprayed alone or in mixture with ametryn) drift simulation on quantitative and qualitative aspects of orange production when applied at two developmental plant stages: flowering and initial fruit development (fruits 2 cm diameter or smaller). Increasing drifts of clomazone, in two formulations, and clomazone in mixtures with ametryn were tested. Fruit morphological features, fruit color, flower and fruit abortion and leaf chlorophyll content were evaluated. Simulated drift similar to the recommended dose of clomazone and clomazone plus ametryn caused fruit abortion in citrus at the stage of fruits 2 cm diameter or smaller. Lower drift doses did not result in fruit abortion. Fruit diameter reduction was observed with drift off above 25% of clomazone alone recommended rate and 50% when in mixture with ametryn or in microcapsule formulation. Clomazone drift at 50% of the recommended rate (alone or in mixture with ametryn ) caused clorotic and necrotic spots in the fruit peel. Drift did not affect juice quality in any of the treatments tested.

Storage of 'Espada' mango fruits in modified atmosphere and cooling: effects on conservation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

In Vitro Antiparasitic Activity and Chemical Composition of the Essential Oil Obtained from the Fruits of Piper cubeba

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Candidatus Liberibacter asiaticus Titers in Citrus and Acquisition Rates by Diaphorina citri Are Decreased by Higher Temperature

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Healing Actions of Essential Oils from Citrus aurantium and d-Limonene in the Gastric Mucosa: The Roles of VEGF, PCNA, and COX-2 in Cell Proliferation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The effect of a minor constituent of essential oil from Citrus aurantium: The role of beta-myrcene in preventing peptic ulcer disease

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The influence of citrus rootstocks on the relationship between the mite Brevipalpus phoenicis and citrus leprosis disease

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A New Species of Maytenus (Celastraceae) with Fleshy Fruits from Eastern Brazil, with Notes on the Delimitation of Maytenus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparative proteomic analysis reveals that T3SS, Tfp, and xanthan gum are key factors in initial stages of Citrus sinensis infection by Xanthomonas citri subsp citri

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ajustes de modelos não lineares de efeitos fixos, com ponderação e misto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)