Genome-wide profiling of the cardiac transcriptome after myocardial infarction identifies novel heart-specific long non-coding RNAs.

AIM: Heart disease is recognized as a consequence of dysregulation of cardiac gene regulatory networks. Previously, unappreciated components of such networks are the long non-coding RNAs (lncRNAs). Their roles in the heart remain to be elucidated. Thus, this study aimed to systematically characterize the cardiac long non-coding transcriptome post-myocardial infarction and to elucidate their potential roles in cardiac homoeostasis. METHODS AND RESULTS: We annotated the mouse transcriptome after myocardial infarction via RNA sequencing and ab initio transcript reconstruction, and integrated genome-wide approaches to associate specific lncRNAs with developmental processes and physiological parameters. Expression of specific lncRNAs strongly correlated with defined parameters of cardiac dimensions and function. Using chromatin maps to infer lncRNA function, we identified many with potential roles in cardiogenesis and pathological remodelling. The vast majority was associated with active cardiac-specific enhancers. Importantly, oligonucleotide-mediated knockdown implicated novel lncRNAs in controlling expression of key regulatory proteins involved in cardiogenesis. Finally, we identified hundreds of human orthologues and demonstrate that particular candidates were differentially modulated in human heart disease. CONCLUSION: These findings reveal hundreds of novel heart-specific lncRNAs with unique regulatory and functional characteristics relevant to maladaptive remodelling, cardiac function and possibly cardiac regeneration. This new class of molecules represents potential therapeutic targets for cardiac disease. Furthermore, their exquisite correlation with cardiac physiology renders them attractive candidate biomarkers to be used in the clinic.

Un nou fragment del sepulcre de Ramon Folc VI de Cardona del monestir de Poblet

En el present article donem a conèixer un fragment del sepulcre de Ramon Folc VI de Cardona (†1320), conegut com el Prohom Vinculador, que es troba al braç meridional del transsepte de l’església del monestir de Poblet. El fragment fou subhastat a Barcelona el març del 2008 i fou adquirit per la Generalitat de Catalunya, que el diposità al Museu de Lleida: diocesà i comarcal. La peça sortia a la venda amb un possible origen pobletana procedent de la tradició antiquària, ja que havia estat propietat del pintor i il·lustrador modernista Alexandre de Riquer (1856-1920), de qui sabem que va extreure materials del monestir, a la vegada que és coneguda la seva faceta de col·leccionista i marxant d’art i antiguitats. Finalment, aquesta procedència s’ha pogut corroborar en descobrir-se que el fragment subhastat era un dels membra disjecta de l’esmentat sepulcre. Avui el fragment es troba pendent de ser restituït al Monestir de Poblet.

Repraesentatio geographica itineris maritimi navis victoriae in qva ex personis CCXXXVII finita navigatione rediere... VI Sept. Ann. MDXXII - 1

Viduae 1703

Plantar flexor neuromuscular adjustments following match-play football in hot and cool conditions.

We assessed neuromuscular fatigue and recovery of the plantar flexors after playing football with or without severe heat stress. Neuromuscular characteristics of the plantar flexors were assessed in 17 male players at baseline and ∼30 min, 24, and 48 h after two 90-min football matches in temperate (∼20 °C and 55% rH) and hot (∼43 °C and 20% rH) environments. Measurements included maximal voluntary strength, muscle activation, twitch contractile properties, and rate of torque development and soleus EMG (i.e., root mean square activity) rise from 0 to 30, -50, -100, and -200 ms during maximal isometric contractions for plantar flexors. Voluntary activation and peak twitch torque were equally reduced (-1.5% and -16.5%, respectively; P < 0.05) post-matches relative to baseline in both conditions, the latter persisting for at least 48 h, whereas strength losses (∼5%) were not significant. Absolute explosive force production declined (P < 0.05) 30 ms after contraction onset independently of condition, with no change at any other epochs. Globally, normalized rate of force development and soleus EMG activity rise values remained unchanged. In football, match-induced alterations in maximal and rapid torque production capacities of the plantar flexors are moderate and do not differ after competing in temperate and hot environments.

Yksittäistuotannon robotisointi konenäön avulla

Työn tavoitteena oli uuden konenäköpohjaisen hitsausrobottiaseman käyttöönotto ja järjestelmän kehittäminen siten, että voitiin mahdollistaa vähintään vaadittu 70 %:n kaariaikasuhde ohjelmien sisällä. Aseman käyttöönotolla pyrittiin tehostamaan hitsaustyötä ja helpottamaan tuotanto-paineita osavalmistuksen paneeli-linjalla. Hitsausasemalla parannetaan myös työntekijöiden työergonomiaa sekä koko työn imagoa. Kirjallisen osan tarkoituksena oli tutkia robotisoitua hitsausta ja sen tuomia etuja, verrata jo olemassa olevia ohjelmointimenetelmiä uuteen ohjelmointitapaan ja selvittää robottien tarkkuuksia. Kirjallisuusosan jälkimmäisessä osassa tutustuttiin konenäköön ja sen laitteistoihin sekä sovelluksiin. Kirjallisuus-tutkimuksessa selvisi, että uusi ohjelmointimenetelmä on selvä parannus kyseiseen sovellukseen. Käytännön osassa on esitelty konenäköpohjainen hitsausrobottiasema sen suunnittelusta, käyttöönottoon ja tuotantotesteihin asti. Käytännön osassa on lisäksi esitelty asema osana koko automatisointiprojektia. Lisäksi on kerrottu järjestelmän toiminnasta sekä makroista, joihin robotin toiminta perustuu. Lopuksi on tehty katsaus maailmalla olevista vastaavista järjestelmistä ja niiden teknologioista sekä verrattiin niitä tähän uuteen järjestelmään. Tavoitteena olleeseen 70% kaariaikasuhteeseen ohjelmien sisällä päästiin jo lyhyen koejakson aikana. Käyttöönotettu konenäköpohjainen hitsausrobottiasema on tiettävästi ensimmäinen hitsausasema maailmassa, jota ei tarvitse ohjelmoida etukäteen. Ohjelmointiin kuluva aika on minimaalinen, koska operaattori ohjelmoi robotin sen hitsatessa tuotetta. Kokeellinen osa osoitti, että käyttöönotettu konenäköpohjainen hitsausrobottiasema toimii, kuten se oli suunniteltukin. Käyttöönoton yhteydessä huomattiin monia kehitettäviä asioita, joilla järjestelmästä on mahdollista saada vieläkin tehokkaampi.

Tissue-Specific Evolution of Protein Coding Genes in Human and Mouse.

Protein-coding genes evolve at different rates, and the influence of different parameters, from gene size to expression level, has been extensively studied. While in yeast gene expression level is the major causal factor of gene evolutionary rate, the situation is more complex in animals. Here we investigate these relations further, especially taking in account gene expression in different organs as well as indirect correlations between parameters. We used RNA-seq data from two large datasets, covering 22 mouse tissues and 27 human tissues. Over all tissues, evolutionary rate only correlates weakly with levels and breadth of expression. The strongest explanatory factors of purifying selection are GC content, expression in many developmental stages, and expression in brain tissues. While the main component of evolutionary rate is purifying selection, we also find tissue-specific patterns for sites under neutral evolution and for positive selection. We observe fast evolution of genes expressed in testis, but also in other tissues, notably liver, which are explained by weak purifying selection rather than by positive selection.

Dysménorrhée: patience, pilules ou bouillotte [Dysmenorrhea: patience, pills or hot-water bottle?].

Which treatments are used for dysmenorrhea and with what reported outcome? A questionnaire was sent to 2400 students and apprentices, following the "retrospective treatment-outcome" method. The response rate was 22%. Most frequent treatments used are ibuprofene (53%), paracetamol (51%), hormonal contraception (40%), hot-water bottle (or hot pad) (35%), food supplements or medicinal plants (23%). Physicians only discuss a tiny proportion of dysmenorrhea treatment in their consultation, because it is mostly a matter of self-treatment, with the family as the source of information in 80% of the cases. Rather surprising because not mentioned in most official guidelines, hot-water bottle (or hot pad) appears as the treatment followed by the best reported outcome (satisfactory in 92% of users).

Phase mask selection in wavefront coding systems: an adaptive approach

A method for optimizing the strength of a parametric phase mask for a wavefront coding imaging system is presented. The method is based on an optimization process that minimizes a proposed merit function. The goal is to achieve modulation transfer function invariance while quantitatively maintaining nal image delity. A parametric lter that copes with the noise present in the captured images is used to obtain the nal images, and this lter is optimized. The whole process results in optimum phase mask strength and optimal parameters for the restoration lter. The results for a particular optical system are presented and tested experimentally in the labo- ratory. The experimental results show good agreement with the simulations, indicating that the procedure is useful.

Manifestações radiológicas da mucopolissacaridose tipo VI

O autor relata as alterações radiológicas da mucopolissacaridose tipo VI (mucopolissacárides VI) observadas em dois irmãos. Foram realizadas radiografias convencionais do esqueleto e estudo por ressonância magnética do sistema nervoso central. As alterações osteoarticulares são bem conhecidas e em geral semelhantes às das outras mucopolissacárides. O estudo por ressonância magnética do sistema nervoso central pode colaborar no diagnóstico diferencial dessas doenças, avaliar a extensão das lesões e ajudar no controle evolutivo.

El recurso a la tiranía como respuesta a la dominación visigoda en la Tarraconense (siglos V-VI)

A finales del siglo V, la población hispanorromana contempló con disgusto el incremento de la presencia visigoda en tierras de la Tarraconense. Entre finales del siglo V e inicios del VI, dos individuos ―Burdunelo y Pedro― se alzaron con el poder de manera ilegal y acaudillaron revueltas contra el dominio visigodo. La fuente que nos informa de estos hechos, la Pseudo Chronica Caesaraugustana, es un texto altamente problemático: interpretado durante mucho tiempo como los restos de la historiola escrita por el obispo Máximo de Zaragoza (c. 599-614/620), en realidad se trata solo de unas anotaciones realizadas al margen de crónicas anteriores. Las vicisitudes de la transmisión manuscrita han provocado, además, el desplazamiento de algunas de estas anotaciones de su posición original, con lo que nos proporcionan dataciones erróneas. En este trabajo estudiamos las noticias relativas a las rebeliones de Burdunelo y de Pedro, realizamos una nueva propuesta de cronología, analizamos sus orígenes sociales y examinamos el alcance que pudieron tener estas sublevaciones en el contexto de un inminente enfrentamiento entre visigodos y francos.

Involvement of long non-coding RNAs in beta cell failure at the onset of type 1 diabetes in NOD mice.

AIMS/HYPOTHESIS: Exposure of pancreatic beta cells to cytokines released by islet-infiltrating immune cells induces alterations in gene expression, leading to impaired insulin secretion and apoptosis in the initial phases of type 1 diabetes. Long non-coding RNAs (lncRNAs) are a new class of transcripts participating in the development of many diseases. As little is known about their role in insulin-secreting cells, this study aimed to evaluate their contribution to beta cell dysfunction. METHODS: The expression of lncRNAs was determined by microarray in the MIN6 beta cell line exposed to proinflammatory cytokines. The changes induced by cytokines were further assessed by real-time PCR in islets of control and NOD mice. The involvement of selected lncRNAs modified by cytokines was assessed after their overexpression in MIN6 cells and primary islet cells. RESULTS: MIN6 cells were found to express a large number of lncRNAs, many of which were modified by cytokine treatment. The changes in the level of selected lncRNAs were confirmed in mouse islets and an increase in these lncRNAs was also seen in prediabetic NOD mice. Overexpression of these lncRNAs in MIN6 and mouse islet cells, either alone or in combination with cytokines, favoured beta cell apoptosis without affecting insulin production or secretion. Furthermore, overexpression of lncRNA-1 promoted nuclear translocation of nuclear factor of κ light polypeptide gene enhancer in B cells 1 (NF-κB). CONCLUSIONS/INTERPRETATION: Our study shows that lncRNAs are modulated during the development of type 1 diabetes in NOD mice, and that their overexpression sensitises beta cells to apoptosis, probably contributing to their failure during the initial phases of the disease.