Separação de cristais de estruvita de percolado de lodo por flotação por ar dissolvido

The struvite precipitation in wastewater treatments plants offer advantages to obtaining a economically viable product, containing phosphorus, nitrogen and magnesium, in a bioavailable conditions to plants. The precipitation process requires ideal conditions, but all of these aspects are difficult to obtain and may result in resistance to collect crystals during the sedimentation process. Therefore, instead find the best conditions for precipitation, we propose the dissolved air flotation as another method to retrieve the crystals. The application of dissolved air flotation method to promote separation of struvite crystals from sewage supernatant, was evaluated in a precipitation-flotation reactor (flotatest) and were compared with the precipitation-sedimentation assays (jar test). Finally, were observed that the dissolved air flotation process was as feasible as sedimentation to promote struvite crystals separation, and the pH are the most influencer factor for an efficient separation

Avaliação da flotação por ar dissolvido no polimento de efluentes de lagoa de estabilização

Experience in the use of stabilization ponds shows that this is a system with low cost, easy operation and maintenance and suitable for tropical countries. One of its disadvantages is due to high concentrations of suspended solids, mainly due to algal biomass in the effluents. Accordingly, the dissolved air flotation has been shown to be an efficient post-treatment technology. However, the efficiency of this process is related to coagulation and flocculation steps, as well as the adjustment of the involved variables in the process. The objective of this work was to evaluate the algae removal efficiency from wastewater stabilization ponds and the influence of the factors involved in the process using dissolved air flotation. For this, we used primary facultative and maturation effluents of ETE Ponta Negra in Natal. We did tests of coagulation, flocculation and flotation with the samples, using the equipment flotatest. In this process were tested coagulants aluminum sulfate and ferric chloride, and varied the factors pH, coagulant concentration, polymer concentration and rate of recirculation. At the end of the experiments were analyzed turbidity, suspended solids, color, COD and chlorophyll "a". These results were submitted to descriptive statistics to verify the efficiency of the process in general, and regression analysis to identify models that describe the process and demonstrate the factors that have greater influence on flotation. After step methodology, high values were found removal efficiency of suspended solids, reaching values greater than 90% in the best cases. It was concluded that flotation is more efficient in the facultative pond effluent using ferric chloride, and the variability of algae may negatively influence the process. Regression analyzes showed that pH is the most influential variable in the coagulation-flocculation-flotation, and its optimal value among the tested is 5.5 for both coagulants

Limpeza da superfície de corte de rebolos por um sistema de ar comprimido na retificação cilíndrica externa de mergulho de materiais cerâmicos refrigerados com a técnica da mínima quantidade de lubrificante (MQL)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Poluentes atmosféricos: monóxido e dióxido de carbono no nordeste do Brasil

The interference of man in the middle atmosphere can be evidenced by the presence of carbon monoxide, gas associated with burning fossil fuels and carbon dioxide content, essential for respiration of plants and thermal balance of the Earth. In this thesis we initially evaluated the intensity of the spatial distribution of carbon monoxide in the Northeast of Brazil, and subsequently the behavior of temporal variations of the pollutants carbon monoxide and carbon dioxide in the atmospheric boundary layer Maxaranguape / RN. Research has shown that, driven by speculation and promoting the occupation of land for agriculture, cattle ranching and tourism in the Northeast of Brazil, the changes established by the man in the middle geomorphological affect the lower troposphere on a large scale, with a predominance of concentrations in central Pernambuco, Paraiba's south-central and central-west of Alagoas. However, the study of Maxaranguape / RN results showed little variation in carbon monoxide and carbon dioxide, with the speed of the wind persisting with values greater than 7.8 m / s, showing dispersion and diffusion of pollutants which resulted in faster renewal of local atmospheric air

Polymorphisms in the AR and PSA Genes as Markers of Susceptibility and Aggressiveness in Prostate Cancer

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Genotyping of AR and PSA polymorphisms in a patient with Klinefelter syndrome, non-Hodgkin lymphoma, and adenocarcinoma of the prostate

It has been hypothesized that the AR (androgen receptor) gene binds the two PSA (prostate-specific antigen) alleles with differing affinities and may differentially influence prostate cancer risk. In this article, we report a case of adenocarcinoma of the prostate in a 56-year-old man with Klinefelter syndrome (47,XXY) and non-Hodgkin lymphoma, as well as the AR and PSA genotype. AR and PSA gene polymorphisms were analyzed by polymerase chain reaction-based methods using DNA from peripheral white blood cells and the prostate cancer. We determined the methylation status of the AR gene on the X chromosome. The patient presents with the AG genotype for the ARE-I (androgen response element) region of the PSA gene. We detect the presence of two short AR alleles with 19 and 11CAG repeats each. Unmethylated alleles were demonstrated for both. The shorter allele was inactive in more than 60% of total DNA in both control blood and prostate cancer cells. The presence of short AR alleles and the G allele of the PSA gene may contribute to the development of prostate cancer in a 47,XXY patient. (C) 2004 Elsevier B.V. All rights reserved.

PSA and androgen-related gene (AR, CYP17, and CYP19) polymorphisms and the risk of adenocarcinoma at prostate biopsy

The aim of the present study was to examine the impact of polymorphisms in prostate-specific antigen (PSA) and androgen-related genes (AR, CYP17, and CYP19) on prostate cancer (PCa) risk in selected high-risk patients who underwent prostate biopsy. Blood samples and prostate tissues were obtained for DNA analysis. Single-nucleotide polymorphisms in the 50-untranslated regions (UTRs) of the PSA (substitution A > G at position -158) and CYP17 (substitution T > C at 50-UTR) genes were detected by polymerase chain reaction (PCR)-restriction fragment length polymorphism assays. The CAG and TTTA repeats in the AR and CYP19 genes, respectively, were genotyped by PCR-based GeneScan analysis. Patients with the GG genotype of the PSA gene had a higher risk of PCa than those with the AG or AA genotype (OR = 3.79, p = 0.00138). The AA genotype was associated with lower PSA levels (6.44 +/- 1.64 ng/mL) compared with genotypes having at least one G allele (10.44 +/- 10.06 ng/mL) (p = 0.0687, 95% CI - 0.3146 to 8.315, unpaired t-test). The multivariate analysis confirmed the association between PSA levels and PSA genotypes (AA vs. AG+GG; chi(2) = 0.0482) and CYP19 (short alleles homozygous vs. at least one long allele; chi(2) = 0.0110) genotypes. Genetic instability at the AR locus leading to somatic mosaicism was detected in one PCa patient by comparing the length of AR CAG repeats in matched peripheral blood and prostate biopsy cores. Taken together, these findings suggest that the PSA genotype should be a clinically relevant biomarker to predict the PCa risk.

Shorter CAG repeat length in the AR gene is associated with poor outcome in head and neck cancer

Objective: Alterations in the size of the [CAG](n) repeats of the AR gene have been described in several types tumors. The purpose of this study was to evaluate if there is an association between the AR [CAG](n) repeat alleles and the relative risk for head and neck cancer and to analyse microsatellite instability (MSI) and loss of heterozygosity (LOH) in these tumors.Design: Matched samples of blood and head and neck tumors were evaluated using two methodologies, silver-stained gels to perform the analyses of MSI and LOH, and automated analysis to confirm these results and for genotyping of the AR [CAG](n), repeat length. Sixty-nine individuals without cancer were used as a control group for both procedures. The Log-rank test was used to compare overall survival and disease-free survival curves. The Cox proportional hazards regression models were performed to determine the [CAG], repeats as an independent prognostic factor.Results: Patients with alleles <= 20 in the male group showed a correlation with lower disease-free survival (P = 0.0325) and with recurrence or metastasis (RR 2.52, CI 95%). in the female group, the allele 2 (longer allele) showed a significant lower mean of [CAG](n), repeat when compared to the control group. Microsatellite instability was detected in nine cases in both procedures. In six out of these nine cases, we observed a reduction of the AR [CAG](n) repeat length. LOH was detected in one out of 17 women informative for oral cancer in both procedures.Conclusion: These results suggest that short [CAG](n) repeat length (: 20) polymorphism is associated with poor prognosis in a subset of male patients with head and neck cancer and that AR gene microsatellite instability is uncommon in these tumors. (C) 2007 Elsevier Ltd. All rights reserved.

Scattering of positronium by H, He, Ne, and Ar

The low-energy scattering of the ortho-positronium (Ps) by H, He, Ne, and Ar atoms has been investigated in the coupled-channel framework by using a recently proposed time-reversal symmetric non-local electron-exchange model potential with a single parameter C. For H and He, we use a three-Ps-state coupled-channel model and, for Ar and Ne, we use a static-exchange model. The sensitivity of the results is studied with respect to the parameter C. Present low-energy cross-sections for He, Ne and Ar are in good agreement with experiment. (C) 2000 Elsevier B.V. B.V. All rights reserved.

Gota suspensa para avaliação de aldeído total no ar interno e externo do ambiente

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atmospheric pollution effects on childhood health: an environmental study in the Paraíba Valley

OBJETIVOS: estimar as correlações entre os níveis de poluentes atmosféricos e o número de internações por doença respiratória em crianças, em hospital universitário. MÉTODOS: trata-se de um estudo ecológico de série temporal. Os dados sobre poluentes atmosféricos foram obtidos junto à CETESB e os dados sobre internação hospitalar, junto ao SAME do Hospital Universitário de Taubaté, Brasil. O estudo se refere a dados e internação do ano de 2001. Para estudar as correlações dos valores dos poluentes entre si e entre as internações, utilizou-se da técnica de correlação de Pearson. Foram estimados os riscos relativos para internação por doença respiratória comparando os quartis dos agentes poluentes com os valores do primeiro quartil. A significância estatística adotada foi alfa = 5%. RESULTADOS: foram internadas 158 crianças com doença respiratória no ano de 2001 (30% do total de internações no ano). Os poluentes estiveram correlacionados entre si e houve correlação positiva, entre o número de internação e dióxido de enxofre e material particulado; houve aumento de 25% no risco de internação comparando o quarto quartil e o primeiro quartil. CONCLUSÕES: houve correlação positiva entre número de internações por doenças respiratórias e poluentes atmosféricos.

SECAGEM DE SEMENTES DE SOJA em SILO COM DISTRIBUIÇÃO RADIAL do FLUXO DE AR: I. MONITORAMENTO FÍSICO

O trabalho foi desenvolvido com o objetivo de avaliar a evolução física do processo de remoção de água das sementes em secador estacionário, com cilindro central perfurado e distribuição radical de ar. A pesquisa foi conduzida com sementes de soja, variando o fluxo (26,9, 28,4 e 33,2 m³/minuto/t) e a temperatura do ar insuflado (42, 46 e 50ºC), considerando a posição das sementes (17, 34 e 51 cm em relação ao cilindro de insuflação) e o tempo de secagem (zero a doze horas, com intervalos de duas horas). Foram caracterizados o ar ambiente, o ar insuflado, as temperaturas e os teores de água da massa, as velocidades e curvas de secagem. As avaliações realizadas destacaram vantagens físicas operacionais da combinação de 28,4 m³/minuto/t com 46ºC e o contrário, com a combinação de 26,9 m³/minuto/t com 42ºC.

SECAGEM DE SEMENTES DE SOJA em SILO COM DISTRIBUIÇÃO RADIAL do FLUXO DE AR: II. EFEITOS SOBRE A QUALIDADE DAS SEMENTES

O trabalho avaliou, em sementes de soja, as conseqüências qualitativas provenientes da secagem estacionária com distribuição radial de ar, variando o fluxo (26,9, 28,4 e 33,2 m³/minuto/t) e a temperatura do ar insuflado (42, 46 e 50ºC), considerando a posição das sementes na massa (17, 34 e 51 cm em relação ao cilindro de insuflação) e o tempo de secagem (0 a 12 horas, com intervalos de quatro horas). Para tanto, além das determinações das temperaturas e dos teores de água da massa, foi avaliado o desempenho fisiológico das sementes no início e ao final de seis meses de armazenamento. Apesar das vantagens físicas operacionais resultantes da combinação entre o fluxo e a temperatura intermediários (28,4 m³/minuto/t e 46ºC), a qualidade fisiológica foi menos prejudicada nas combinações dos menores fluxos (26,9 e 28,4 m³/minuto/t) com a maior temperatura (50ºC) e do maior fluxo (33,2 m³/minuto/t) com as menores temperaturas (42 e 46ºC); entre estas, levando em conta os aspectos físico-operacionais, a associação de 28,4 m³/minuto/t com 50ºC foi a mais eficiente na retirada de água das sementes. Assim, admitindo os intervalos de fluxo (26,9 a 33,2 m³/minuto/t) e de temperatura (42 a 50ºC) estudados, conclui-se que a elevação na temperatura demanda redução no fluxo e, inversamente, o aumento no fluxo demanda redução na temperatura.

Altitude e coordenadas geográficas na estimativa da temperatura mínima média decendial do ar no estado do Rio Grande do Sul

O objetivo deste trabalho foi verificar, com dados de temperatura mínima média decendial do ar (Tm) de 41 municípios do Estado do Rio Grande do Sul, de 1945 a 1974, se a Tm pode ser estimada em função da altitude, latitude e longitude. Para cada um dos 36 decêndios do ano, realizaram-se análise de correlação, análise de trilha das variáveis causais - altitude, latitude e longitude - sobre o efeito Tm, e estimaram-se os parâmetros do modelo das equações de regressão linear múltipla, pelo método passo a passo, com teste para saída de variáveis, considerando Tm como variável dependente e altitude, latitude e longitude como variáveis independentes. Na validação dos modelos de estimativa da Tm, usou-se o coeficiente de correlação linear de Pearson, entre a Tm estimada e a Tm observada em dez municípios do Estado, com dados da série de observações meteorológicas de 1975 a 2004. A temperatura mínima média decendial do ar pode ser estimada pelas coordenadas geográficas em qualquer local e decêndio, no Estado do Rio Grande do Sul. A altitude e latitude explicam melhor a variação da Tm.