Murine Model for Preclinical Studies of Var2CSA-Mediated Pathology Associated with Malaria in Pregnancy

Plasmodium falciparum infection during pregnancy leads to abortions, stillbirth, low birth weight, and maternal mortality. Infected erythrocytes (IEs) accumulate in the placenta by adhering to chondroitin sulfate A (CSA) via var2CSA protein exposed on the P. falciparum IE membrane. Plasmodium berghei IE infection in pregnant BALB/c mice is a model for severe placental malaria (PM). Here, we describe a transgenic P. berghei parasite expressing the full-length var2CSA extracellular region (domains DBL1X to DBL6ε) fused to a P. berghei exported protein (EMAP1) and characterize a var2CSA-based mouse model of PM. BALB/c mice were infected at midgestation with different doses of P. berghei-var2CSA (P. berghei-VAR) or P. berghei wild-type IEs. Infection with 10(4) P. berghei-VAR IEs induced a higher incidence of stillbirth and lower fetal weight than P. berghei At doses of 10(5) and 10(6) IEs, P. berghei-VAR-infected mice showed increased maternal mortality during pregnancy and fetal loss, respectively. Parasite loads in infected placentas were similar between parasite lines despite differences in maternal outcomes. Fetal weight loss normalized for parasitemia was higher in P. berghei-VAR-infected mice than in P. berghei-infected mice. In vitro assays showed that higher numbers of P. berghei-VAR IEs than P. berghei IEs adhered to placental tissue. Immunization of mice with P. berghei-VAR elicited IgG antibodies reactive to DBL1-6 recombinant protein, indicating that the topology of immunogenic epitopes is maintained between DBL1-6-EMAP1 on P. berghei-VAR and recombinant DBL1-6 (recDBL1-6). Our data suggested that impairments in pregnancy caused by P. berghei-VAR infection were attributable to var2CSA expression. This model provides a tool for preclinical evaluation of protection against PM induced by approaches that target var2CSA.

Age model and stable isotopes of sediment core RC17-177

A careful comparison is made between the most detailed records of sea level over the last glacial cycle, and two high-quality oxygen isotope records. One is a high-resolution benthonic record that contains superb detail but proves to record temperature change as well as ice volume; the other is a planktonic record from the west equatorial Pacific where the temperature effect may be minimal but where high resolution is not available. A combined record is generated which may be a better approximation to ice volume than was previously available. This approach cannot yet be applied to the whole Pleistocene. However, comparison of glacial extremes suggests that glacial extremes of stages 12 and 16 significantly exceeded the last glacial maximum as regards ice volume and hence as regards sea level lowering. Interglacial stages 7, 13, 15, 17 and 19 did not attain Holocene oxygen isotope values; possibly the sea did not reach its present level. It is unlikely that sea level was glacio-eustatically higher than present by more than a few metres during any interglacial of the past 2.5 million years.

Age model and stable isotopes of sediment cores from north-central Chile and south-central Chile

Terrigenous sediment supply, marine transport, and depositional processes along tectonically active margins are key to decoding turbidite successions as potential archives of climatic and seismic forcings. Sequence stratigraphic models predict coarse-grained sediment delivery to deep-marine sites mainly during sea-level fall and lowstand. Marine siliciclastic deposition during transgressions and highstands has been attributed to sustained connectivity between terrigenous sources and marine sinks facilitated by narrow shelves. To decipher the controls on Holocene highstand turbidite deposition, we analyzed 12 sediment cores from spatially discrete, coeval turbidite systems along the Chile margin (29° - 40°S) with changing climatic and geomorphic characteristics but uniform changes in sea level. Sediment cores from intraslope basins in north-central Chile (29° - 33°S) offshore a narrow to absent shelf record a shut-off of turbidite deposition during the Holocene due to postglacial aridification. In contrast, core sites in south-central Chile (36° - 40°S) offshore a wide shelf record frequent turbidite deposition during highstand conditions. Two core sites are linked to the Biobío river-canyon system and receive sediment directly from the river mouth. However, intraslope basins are not connected via canyons to fluvial systems but yield even higher turbidite frequencies. High sediment supply combined with a wide shelf and an undercurrent moving sediment toward the shelf edge appear to control Holocene turbidite sedimentation and distribution. Shelf undercurrents may play an important role in lateral sediment transport and supply to the deep sea and need to be accounted for in sediment-mass balances.

The death penalty; a report for the Model penal code project of the American Law Institute.

"Reprinted from tentative draft no. 9 of the Model penal code."

Study of activated sludge separation by dynamic straining /

Mode of access: Internet.

Pyrolysis of industrial wastes for oil and activated carbon recovery /

Issued May 1977.

Los Angeles and Long Beach Harbors model study : numerical analysis of tidal circulation for the 2020 Master Plan /

"June 1984."

Los Angeles and Long Beach Harbors model study : resonant response of the harbors for Phase 1 of the Los Angeles deep-draft dry bulk export terminal /

"January 1984."

The model architect : a series of original designs for cottages, villas, suburban residences, etc., accompanied by explanations, specifications, estimates, and elaborate details, prepared expressly for the use of projectors and artisans throughout the United States /

Mode of access: Internet.

Chris, the model maker; a story of New York,

Mode of access: Internet.

A model for the identification of high risk occupational groups using RTECS and NOHS data /

Accompanying microfiches (issued in pocket inside back cover) have title: NIOSH risk identification model.

"Model T": a demonstration of image multiplication using stochastic sequences,

On cover: C00-1469-145.