GAS-PHASE REACTIONS OF C-60 WITH THE ION SYSTEMS OF CYCLOHEXANE AND CYCLOHEXENE

The DCI MS of C-60 with the reactions of gaseous cyclohexane and cyclohexene have been studied. Several kinds reactions of C-60 have been observed. The results show that the gasphase C-60 has very active chemical properties. The adduct ions of [C60C4H7](+) and [C60C5H7](+) may be formed by [2+4] cycloaddition where one of double bonds of C-60 acted as a dienophilic unit.

SYNTHESIS OF A NEW CHIRAL LIQUID-CRYSTAL WITH SCHIFF-BASE GROUP AND INVESTIGATION OF TEMPERATURE-DEPENDING FTIR SPECTRA

A new chiral liquid crystal with Schiff base group has been prepared, The structure of liquid crystal was confirmed by elementary analyses and H-1 NMR. Its phase transition was investigated by polarized optical microscope, DSC and temperature-depending FTIR spectra. The results showed that the chiral Schiff base showed monotropic phases behavior in certain temperature range, the phase sequence is I-N-*-S-B-S-G-K on the cooling sequence.

Photovoltaic Properties and Quantum Size Effect in Nanocrystalline CeO2

Different size. nanocrystallines CeO2 were synthesized and their diffuse reflectance absorption spectra have heen measured. The absorption band in the region from 300 to 450 nm was assigned to the O2p-Ce2 4f transition. It was found that a strongly red-shifted broad continuum of the absorption band occured as the decrease of the partical size. We have also measured the surface photovoltage as function of wavelength by SPS technique. And the absorption band was resolved to two peaks with different photovoltaic properties. Photovoltaic quantum size effect was observed by FMSPS measurement.

新的手性Schiff碱液晶的合成及变温红外光谱研究（Ⅰ）

合成了１个新的手性Ｓｃｈｉｆｆ碱类液晶并以ＤＳＣ、偏光显微镜及变温红外光谱等手段对其热致相变过程进行了研究。

C_(60)与环己烷及环己烯离子体系的气相反应

在气相条件下研究了C_(60)与环己烷及环己烯的离子-分子反应.C_(60)可与上述体系中多种离子发生反应,生成相应的加合离子,表现出C_(60)化学性质的活泼性和多样性.C_(60)与C_4H_7~+和C_5H_7~+离子反应可能生成[2+4]环加成的加合离子.

A STUDY ON THE INDICATION OF UNEXPECTED COMPONENTS BY KALMAN FILTERING IN FLUOROSPECTROPHOTOMETRY

The potential of Kalman filtering for indication of unexpected components in a mixture was experimentally evaluated by taking the spectrofluorimetric analysis of the tricomponent system;oi phenylalanine, tryhtophen and tyrosine as an example. According to

荧光光度法中Kalman滤波法指示未知组分的研究

以苯丙氨酸、酪氨酸和色氨酸三组分体系的荧光光度分析为例,研究了Kalman滤波法指示未知组分的潜力.根据新息序列的白噪音特性,建立指示函数,并确定指示函数的临界值,指示未知组分存在;并根据相对新息序列的形状可大致估计未知组分的荧光光谱的形状和峰位.

STUDIES ON OLEFIN-COORDINATING TRANSITION-METAL CARBENE COMPLEXES .20. SYNTHESIS OF 1,3-CYCLOHEXADIENE(DICARBONYL) [ETHOXY(ARYL)-CARBENE]IRON COMPLEXES AND THEIR PHOSPHINE ADDUCTS - CRYSTAL-STRUCTURE OF C6H8(CO)2FEC(OC2H5)C6H4CH3-O

Reaction of 1,3-cyclohexadiene(tricarbonyl)iron (1) with ortho-substituted aryllithium reagents ArLi (Ar=o-CH3C6H4, o-CH3OC6H4, o-CF3C6H4) in ether at low temperature, and subsequent alkylation of the acylmetalates formed with Et3OBF4 in aqueous solution at 0-degrees-C or in CH2Cl2 at -60-degrees-C gave the 1,3-cyclohexadiene(dicarbonyl)[ethoxy(aryl)carbene]iron complexes (eta4-C6H8)(CO)2FeC(OC2H5)Ar (3, Ar = o-CH3C6H4; 4, Ar = o-CH3OC6H4), and the isomerized product (eta3-C6H8)(CO)2FeC(OC2H5)C6H4CF3-o (5), respectively, among which the structure of 3 has been established by an X-ray diffraction study. Complex 3 is monoclinic, space group P2(1) with a = 8.118(4), b = 7.367(4), c = 14.002(6) angstrom, beta = 104.09(3)-degrees, V = 812.2(6) angstrom3, Z = 2, D(c) = 1.39 g cm-3, R = 0.056, and R(w) = 0.062 for 976 observed reflections. Complexes 3 and 5 were converted into the chelated allyliron phosphine adducts(eta3-C6H8)(CO)2(PR31)FeC(OC2H5)Ar (6, Ar = o-CH3C6H4, R1 = Ph; 7, Ar = o-CH3C6H4, R1 = OPh; 9, Ar = o-CF3C6H4, R1 = Ph), by reaction with phosphines in petroleum ether at low temperatures.

A novel anti-tumor protein extracted from Meretrix meretrix Linnaeus induces cell death by increasing cell permeability and inhibiting tubulin polymerization

Discovery and development of new pharmaceuticals from marine organisms are attracting increasing interest. Several agents derived from marine organisms are under preclinical and clinical evaluation as potential anticancer drugs. We extracted and purified a novel anti-tumor protein from the coelomic fluid of Meretrix meretrix Linnaeus by ammonium sulphate fractionation, ion exchange and hydrophobic interaction chromatography. The molecular weight of the highly purified protein, designated MML, was 40 kDa as determined by SDS-PAGE analysis. MML exhibited significant cytotoxicity to several cancer cell types, including human hepatoma BEL-7402, human breast cancer MCF-7 and human colon cancer HCT116 cells. However, no inhibitory effect was found when treating murine normal fibroblasts NIH3T3 and benign human breast MCF-10A cells with MML. The cell death induced by MML was characterized by cell morphological changes. The induction of apoptosis of BEL-7402 cells by MML was weak by DNA ladder assay. The possible mechanisms of its anti-tumor effect might be the changes in cell membrane permeability and inhibition of tubulin polymerization. MML may be developed as a novel, highly selective and effective anti-cancer drug.

The genetic analysis and germplasm identification of the gametophytes of Undaria pinnatifida (Phaeophyceae) with RAPD method

Eleven pairs of Undaria pinnatifida (Harv.) Suringar gametophytes were identified with random amplified polymorphic DNA (RAPD) technique. After screening 100 primers, 20 ten-base primers were determined for the RAPD analysis. A total of 312 polymorphic loci were obtained, of which 97.7% were polymorphic. The primer S198 was found to distinguish all the selected Undaria pinnatifida gametophytes. The genetic distances between each two of the twenty-two U. pinnatifida gametophytes ranged from 0.080 to 0.428, while the distances to the Laminaria was 0.497 on average. After reexamination, two sequences characterized amplification region (SCAR) markers were successfully converted, which could be applied to U. pinnatifida germplasm identification. All these results demonstrated the feasibility of applying RAPD markers to germplasm characterization and identification of U. pinnatifida gametophytes, and to provide a molecular basis for Undaria breeding.

Palaeoenvironmental changes from pollen record in deep sea core PC-1 from northern Okinawa Trough, East China Sea during the past 24 ka

A pollen record of core PC-1 from the northern Okinawa Trough, East China Sea (ECS), provides information on vegetation and climate changes since 24 cal. kaBP. A total of 103 samples were palynologically analyzed at 8 cm intervals with a time resolution of 230 a. Four pollen zones are recognized: zone I (812-715 cm, 24.2-21.1 cal. kaBP), zone II (715-451 cm, 21.1-15.2 cal. kaBP), zone III (451-251 cm, 15.2-10.8 cal. kaBP), zone IV (251-0 cm, 10.8-0.3 cal. kaBP), corresponding to Late MIS 3, Last Glacial Maximum (LGM), deglaciation and Holocene, respectively. The LGM is characterized by the dominance of herbs, mainly Artemisia, and high pollen influx, implying an open vegetation on the exposed continental shelf and a cool and dry climate. The deglaciation is a climate warming stage with Pinus percentage increased and Artemisia percentage decreased and a rapid sea-level rise. The Holocene is characterized by predominance of tree pollen with rapid increase in Castanea-Castanopsis indicating the development of mixed evergreen and deciduous broad-leaved forest and a warm, humid climate. Low pollen influx during the Holocene probably implies submergence of the continental shelf and retreat of the pollen source area. The vegetation indicated by pollen assemblage found in this upper zone is consistent with the present vegetation found in Kyushu, Japan. Originating from the humid mountain area of North Luzon of the Philippines, Tasmania and New Zealand, Phyllocladus with sporadic occurrence throughout PC-1 core probably suggests the influence of Palaeo-Kuroshio Current or intense summer monsoon. The observed changes in Pinus and Herbs percentage indicate fluctuations of the sea level, and high Pinus percentage corresponds to high sea level. Spectrum analysis of the pollen percentage record reveals many millennial-scale periodicities, such as periodicities of 6.8, 3.85 2.2, 1.6 ka.

辽东湾斑海豹PHOCA LARGHA的年龄鉴定、种群动态和资源保护的初步研究

本文通过对个体年龄鉴定方法的研究，初步分析了辽东湾斑海豹种群的年龄组成、种群动态及数量变化与环境条件的关系。（1）以已知年龄斑海豹（自2个月至24龄）牙齿中的年生长层组为标准，通过计数未知年龄个体牙齿中的年生长层组，准确鉴定了个体年龄，绘制出年龄－体长曲线。活体的大致年龄可通过比较体长而获得。以犬齿中间位置的横切片做年龄鉴定效果最佳。2龄时齿腔根部开始封闭，4 ～ 5龄时全部齿腔为牙齿质充满，故4龄前个体的年龄主要依据牙齿质中的年生长层组，4龄后主要依据牙骨质中的年生长层组。（2）根据种群的年龄结构推知，种群的净增殖率R_0为1.0605，平均世代时间T为6.9149年，内禀增长能力r_m为0.00849/年，周限增长率λ为1.00823年。4、5、6、7龄的雌海豹繁殖价最大，对种群的数量有较大影响。1龄以上斑海豹的死亡曲线仅在接近生理寿命处有一个死亡高峰。（3）斑海豹数量的急剧下降与栖息地生境的改变和人为滥捕有关。但由于R_0 = 1.0605 > 1，所以只要加以妥善保护，种群数量会上升。

A new poly brominated dibenzylphenol from Rhodomela confervoides

A new poly brominated dibenzylphenol named as rhodomevoidin was isolated from Rhodomela confervoides. Its structrue was elucidated as 3-bromo-4, 5-bis (2, 3-dibromo-4, 5-dihydroxybenzyI)pyrocatechol by spectroscopic methods including IR, HRFABMS, 1D and 2D NMR techniques.

青海湖地区芨芨草草地土壤种子库的初步研究

选择青海湖地区的次生芨芨草草地为研究对象,以围栏内封育和围栏外自由放牧的草地做对照,研究了围栏内外土壤种子库的萌发状况、密度和数量、物种多样性,以及土壤种子库与地上植被的关系。结果表明,围栏内外土壤种子库的萌发趋势非常相似,但是萌发种子累计数量不同;围栏内外土壤种子库中萌发种子的平均密度分别为3660和2460粒·m-2,围栏内萌发种子数比围栏外提高了48.8%;围栏内外土壤种子库的物种多样性指数差异较大,围栏内外丰富度指数R1分别为17和13,R2为3.693和2.832,丰富度围栏内大于围栏外,多样性指数和丰富度指数具有相同的规律,均匀度指数围栏内外分别为0.812和0.857,则是围栏外大于围栏内。围栏内外地上植被群落组成存在显著差异,围栏内的封育草地的物种丰富度和多样性指数均大于围栏外的放牧草地,而均匀度指数则呈相反趋势。

祁连獐牙菜化学成分研究

目的 研究祁连獐牙菜Swertia przewalskii全草的化学成分。方法 采用硅胶柱层析进行分离纯化，通过波谱方法及化学关联进行结构鉴定；结果 分离纯化出15个化合物，分别鉴定为：1，7-二羟基-3，8-二甲氧基San酮（Ⅰ），1，8-二羟基-3，7-二甲氧基San酮（Ⅱ），1，7，8-三羟基-3-甲氧基San酮（Ⅲ），1，3，7，8-四羟基San酮（Ⅳ），1-O-β-D-吡喃葡萄糖基-7-羟基-3，8-二甲氧基San酮（Ⅴ），1-O-β-D-吡喃葡萄糖基-8-羟基-3，7-二甲氧基San酮（Ⅵ），7-O-β-D-吡喃葡萄糖基-1，8-二羟基-3-甲氧基San酮（Ⅶ），1-O-[β-D-吡喃木糖（1→6)-β-D-吡喃葡萄糖]-7-羟基-3，8-二甲氧基San酮（Ⅷ），1-O-[β-D-吡喃葡萄糖]-1，7-二羟基-3-甲氧基S sgmk(X),木犀草素（Ⅺ），齐墩果酸（Ⅻ），乌苏酸（XⅣ）和龙胆苦苷（XV）。结论 化合物Ⅳ-Ⅷ，Ⅺ,Ⅻ,XⅣ和XV均为首次从该种植物中分得。