天然雌核发育银鲫卵子控制异源精核发育的受精学机制

作者对两性融合生殖鱼和雌核发育银鲫脱膜卵受精的精核发育进行了观察，并采用鱼类卵子无细胞系对以上两类卵质提取物体外诱导经Ｔｒｉｔｏｎ—Ｘ１００处理的精子及其发育进行了初步研究，结果表明在两性融合生殖型脱膜鱼卵中精核通过解凝最终形成原核，而在雌核发育的银鲫脱膜卵子中部分精核体积虽有一定程度的增加，但始终没有观察到原核的发育；在体外诱导实验中，经Ｔｒｉｔｏｎ—Ｘ１００处理的精子在两类卵质提取物中充分发育，都出现了类似体内原核的状态。该现象提示在银鲫卵质中存在有促使精核形成原核的因子，但在正常受精状态下，由于银鲫

拟腹吸鳅属鱼类颏吸附器的扫描电镜观察及其亚属划分

扫描电镜下，构成拟腹吸鳅鱼类颏吸附器的若干条皮脊均由许多分别根植于单个上皮细胞表面的爪形突组成。爪形突高１５～２０μｍ，横截面常呈椭圆形，长短轴的４～５μｍ，易脱落，也可增生。它们可能具有吸附基质表面、保护周围味蕾等功能。由于爪形突分布部位的不同和所组成皮脊的形态差异，可明显地分为叠波型和品字型两类。这两类吸附器的种类间同时在味蕾分布、口角须结构和背鳍条及腹鳍条数目等方面也存在相应的差异，代表了两个独立演化的自然类群。本文把腹吸鳅属分成两个亚属，将其中颏吸附器呈品字型的三个种和亚种分离出来，成立品唇吸鳅亚

异育银鲫对鱼粉等11种饲料中磷的利用率

于1988年,以三氧化二铬间接指示法,在水温22±2℃条件下,测定了平均体重为55±5g/尾的异育银鲫对鱼粉、肉骨粉、芝麻饼、虾粉、骨粉、菜籽饼、玉米粉、脱氟磷酸氢钙、磷酸二氢钙、磷酸氢钙、磷酸钙的表观消化吸收率。结果表明:异育银鲫对第一、第二、第三磷酸钙的消化吸收率是不同的,分别为81％,65％和32％,此顺序与其溶解性由大到小的顺序一致;在所测试的八种商品饲料中,对骨粉、虾粉和菜籽饼中磷的表观消化吸收率较高,分别为65％,68％和65％;对鱼粉、芝麻饼、肉骨粉三种原料中磷的表观消化吸收率较低,分别是1

以底栖动物评价湘江污染的研究

湘江底栖动物种类较丰富,而上游种类尤多,生物多样性明显。中、下游某些江段因污染等因素影响,多样性有差异。shannon多样性指数反映出了这些变化。上游江段属于清洁水体,中、下游多数江段属于轻污染,少数江段为中污染,而霞湾港江段为重污染。进行了水和底泥中化学毒物的含量与生物主要种类的数量变化的相关分析。

丝藻目的一新属——骈丝藻属

<正> 1976年6月至8月,中国科学院青藏高原综合科学考察队藏北分队对冈底斯山,念青唐古拉山以北,昆仑山以南的广大藏北地区进行了多学科的综合考察。我所陈宜瑜同志参加了这次考察,对该地区各种水体的水生生物进行了广泛地采集,在采得的标本中,藻类是十分丰富的。我们在鉴定该区振泉湖藻类标本时,发现一特殊类型的藻类。通过对液浸标本和固定染色标本的研究,确定它是绿藻门丝藻目的一新属。

黑龙江及达赖湖的水化学材料

<正> 一、材料和方法黑龙江水化学分析是1958年在黑河断面进行的。断面位置、分层分点、采样时间等都与浮游生物的断面采集相同,同时进行。表层采样用改良北原式采水器;底层采样用茹可夫斯基式采水器,用来分析气体的水样则取自缚在茹可夫斯基式采水器上的带出水管的玻瓶采水器中。测定项目共16项——pH、O_2、CO_2、硷度、溶解有机物耗氧量、PO_4~=、NO_3~-、NO_2~-、NH_4~+、Fe~(+++)、总铁量、SiO_2~=、Mg~(++)、总硬度、Cl~-和SO_4~=。最后四个分析项目(Mg~(+

Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper

Aromatase plays a key role in sex differentiation of gonads. In this study, we cloned the full-length cDNA of ovarian aromatase from protogynous hermaphrodite red-spotted grouper (Epinephelus akaara), and prepared the corresponding anti-EaCyp19a1a antiserum. Western blot and immunofluorescence studies revealed ovary-specific expression pattern of EaCyp19a1a in adults and its dynamic expression change during artificial sex reversal. EaCyp19a1a was expressed by follicular cells of follicular layer around oocytes because strong EaCyp19a1a immunofluorescence was observed in the cells of ovaries. During artificial sex reversal, EaCyp19a1a expression dropped significantly from female to male, and almost no any positive EaCyp19a1a signal was observed in testicular tissues. Then, we cloned and sequenced a total of 1967 bp T-flanking sequence of EaCyp19a1a promoter, and showed a number of potential binding sites for some transcriptional factors, such as SOX5, GATA gene family, CREB, AP1, FOXL1, C/EBP, ARE and SF-1. Moreover, we prepared a series of 5' deletion promoter constructs and performed in vitro luciferase assays of EaCyp19a1a promoter activities. The data indicated that the CREB regulation region from -1010 to -898 might be a major cis-acting element to EaCyp19a1a promoter, whereas the elements GATA and SOX5 in the region from -1216 to -1010 might be suppression elements. Significantly, we found a common conserved sequence region in the fish ovary-type aromatase promoters with identities from 93% to 34%. And, the motifs of TATA box, SF-1, SOX5, and CREB existed in the region and were conserved among the most of fish species. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

How polluted is the Yangtze River? Water quality downstream from the Three Gorges Dam

The concentrations of major anions and cations, nitrogen and phosphorus, dissolved and particulate trace elements, and organic pollutants were determined for the middle and lower reaches of the Yangtze River (Changjiang) from below the Three Gorges Dam (TGD) to the mouth at Shanghai in November 2006. The concentration of dissolved inorganic phosphate (DIP) was constant at a low level of 6-8 mu gP/L, but the concentration of nitrate (NO3-) approximately doubled downstream and was closely correlated with K+. This translated to a daily load of well over 1000 It of dissolved inorganic nitrogen (DIN) at Datong. The average concentrations of dissolved Pb (0.078 +/- 0.023 mu g/L), Cd (0.024 +/- 0.009 mu g/L), Cr(0.57 +/- 0.09 mu g/L), Cu (1.9 +/- 0.7 mu g/L), and Ni (0.50 +/- 0.49 mu g/L) were comparable with those in other major world rivers, while As (3.3 +/- 1.3 mu g/L) and Zn (1.5 +/- 0.6 mu g/L) were higher by factors of 5.5 and 2.5, respectively. The trace element contents of suspended particles of As (31 +/- 28 mu g/g), Pb (83 +/- 34 mu g/g), and Ni (52 +/- 16 mu g/g) were close to maximum concentrations recommended for rivers by the European Community (EC). The average concentrations of Cd (2.6 +/- 1.6 mu g/g), Cr (185 +/- 102 mu g/g), Cu (115 +/- 106 mu g/g), and Zn (500 +/- 300 mu g/g) exceeded the EC standards by a factor of two, and Hg (4.4 +/- 4.7 mu g/g) by a factor of 4 to 5. Locally occurring peak concentrations exceed these values up to fourfold, among them the notorious elements As, Hg, and Tl. All dissolved and particulate trace element concentrations were higher than estimates made twenty years ago [Zhang, J., Geochemistry of trace metals from Chinese river/estuary systems: an overview. Estuar Coast Shelf Sci 1995; 41: 631-658.]. The enormous loads of anthropogenic pollutants disposed to the river were diluted by the large water discharge of the Yangtze even during the lowest flow resulting in the relatively low concentration levels of trace elements and organic pollutants observed. We estimated loads of e.g. As, Pb and Ni to the East China Sea to be about 4600 kg As d(-1), 3000 kg Pb d(-1), and 2000 kg Ni d(-1). About 6000 t d(-1) of dissolved organic carbon (DOC) was delivered into the sea at the time of our cruise. We tested for 236 organic pollutants, and only the most infamous were found to be barely above detection limits. We estimated that the load of chlorinated compounds, aromatic hydrocarbons, phenols, and PAHs were between 500 and 3500 kg d(-1). We also detected eight herbicides entering the estuary with loads of 5-350 kg d(-1). The pollutant load, even when at low concentrations, are considerable and pose an increasing threat to the health of the East China Sea ecosystem. (c) 2008 Elsevier B.V. All rights reserved.

Isolation and characterization of polymorphic microsatellites in a sex-reversal fish, rice field eel (Monopterus albus)

The rice field eel (Monopterus albus) is a fish of economic importance in China and some Asian countries. From a (GT)(n)-enriched genomic library, 30 microsatellites were developed by employing the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Thirteen loci exhibited polymorphism with two to 13 alleles (mean 7.9 alleles/locus) in a test population and observed heterozygosity ranged from 0.3125 to 0.9688 (mean 0.7140). These loci should provide sufficient level of genetic variation to study the fine-scale population structure and reproductive ecology of the species.

Development of the microbial communities in Lake Donghu in relation to water quality

There is increasing recognition that protozoa is very useful in monitoring and evaluating water ecological healthy and quality. In order to study the relationship between structure and function of protozoan communities and water qualities, six sampling stations were set on Lake Donghu, a hypereutrophic subtropical Chinese lake. Microbial communities and protists sampling from the six stations was conducted by PFU (Polyurethane foam unit) method. Species number (S), diversity index (DI), percentage of phytomastigophra, community pollution value (CPV), community similarity and heterophy index (HI) were mensurated. The measured indicators of water quality included total phosphorus (TP), dissolved oxygen (DO), Chemical oxygen demand (COD), NH4 (+), NO2 (-) and NO3-. Every month water samples from stations I, II, III, IV were chemically analyzed for a whole year, Among the chemically analyzed stations, station I was the most heavily polluted, station II was the next, stations III and IV had similar pollution degrees. The variable tendencies of COD, TP, NH3, NO2-, NO3-, and DO during the year was approximately coincident among the six stations. Analysis from the community parameters showed that the pollution of station 0 was much more serious than others, and station V was the most slight. Of the community parameters, CPV and HI were sensitive in reflecting the variables of the water quality. Community similarity index was also sensitive in dividing water qualities and the water quality status of different stations could be correctly classified by the cluster analysis. DI could reflect the tendency of water quality gradient, species number and percentage of Phytomastigophora was not obvious in indicating the water quality gradient.

Study on fluorescence emission and synchronous-scan fluorescence spectrea of Nitzschia hantzschiana solution with FE(III)

The characterization of the algal Nitzschia hantzschiana solution with (or without) Fe(III) was carried out using fluorescence emission and synchronous-scan spectroscopy. An emission peak (excited at 440 nm) was observed at 675 nm for Nitzschia hantzschiana solution. The effective characterization method used was synchronous-scan fluorescence spectroscopy (SFS). A wavelength difference (Delta lambda) of 90 nm was maintained between excitation and emission wavelengths. The peak was observed at about 236(ex) nm (326(em) nm) for synchronous fluorescence spectroscopy. Fe(III) was an effective quencher. The relationship between I-0/I (quenching efficiency) and c (concentration of Fe (III) added) was a linear correlation for the algal solution with Fe(III). Effects of pH on synchronous-scan fluorescence intensity were evident.

Determination of 266 pesticide residues in apple juice by matrix solid-phase dispersion and gas chromatography-mass selective detection

A macro matrix solid-phase dispersion (MSPD) method was developed to extract 266 pesticides from apple juice samples prior to gas chromatography-mass selective detection (GC-MSD) determination. A 10 g samples was mixed with 20 g diatomaceous earth. The mixture was transferred into a glass column. Pesticide residues were leached with a 160 mL hexane-dichloromethane (1:1) at 5 mL/min. Two hundred and sixty-six pesticides were divided into three groups and detected by GC-MSD under selective ion monitoring. The proposed method takes advantage of both liquid-liquid extraction and conventional MSPD methods. Application was illustrated by the analysis of 236 apple juice samples produced in Shaanxi province China mainland this year. (C) 2004 Elsevier B.V. All rights reserved.

RecA-mediated, targeted mutagenesis in zebrafish

We have evaluated the efficacy of RecA, a prokaryotic protein involved with homologous recombination, to direct site-specific mutagenesis in zebrafish embryos. For this we coinjected a vector containing a mutated enhanced green fluorescent protein (EGFP) gene plus 236-nucleotide corrective single-stranded DNAs coated with RecA into I-cell zebrafish embryos. Twenty-hours after fertilization, about 5% to 20% of injected embryos showed EGFP expression in I or more cells when RecA-coated corrective DNAs were used, but not when RecA was omitted. Mutated EGFP genes with 1-bp insertions or deletions were inefficiently activated, whereas those with 7-bp insertions were activated about 4-fold more efficiently. RecA-coated template strand had a higher efficiency than its complementary strand in activation of EGFP expression. Prior irradiation of the embryos with UV light enhanced RecA-mediated restoration of gene activity, suggesting that the effects we observed were augmented by one or more factors of zebrafish DNA repair systems.