994 resultados para 680 Manufactura para usos específicos
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Estudo do meio físico e caracterização da capacidade de suporte natural da região de Pirassununga/SP
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring-hydroxylating dioxygenases (alpha-A RH D bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirao Experimental Station secondary forest (SF) and agriculture (AG)-, and the biochar (SF_BC and AG_BC, respectively). Heterogeneity estimates indicated greater diversity in BC libraries; and Venn diagrams showed more unique operational protein clusters (OPC) in the SF_BC library than the ADE soil, which indicates that specific metabolic processes may occur in biochar. Phylogenetic analysis showed unidentified dioxygenases in ADE soils. Libraries containing functional gene encoding for the alpha subunit of the aromatic ring-hydroxylating dioxygenases (ARHD) gene from biochar show higher diversity indices than those of ADE under secondary forest and agriculture.
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Se comparan dos métodos de detección del sistema de transporte de electrones (Båmastedt, 2000 y Owens & King, 1975), hallando que la no adición de sustratos específicos, propios del sistema, no es lo suficientemente sensible para la detección de la capacidad respiratoria y que no presenta ninguna relación con la biomasa. Se demuestra que el receptor artificial de electrones INT, es reducido en reacciones no enzimáticas por algunas sustancias (ácido ascórbico, glutatión, cisteína y potencialmente la vitamina B12), presentes en concentraciones intracelulares.
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Descripción y justificación de los procesos y efectos sedimentarios en las playas arenosas.
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Estudio comparativo sobre las ventajas e inconvenientes de la escolarización en aulas enclave y centros específicos