Prognostic Implication of Lymphovascular Invasion Detected by Double Immunostaining for D2-40 and MITF1 in Primary Cutaneous Melanoma.

BACKGROUND Lymphovascular invasion (LVI) is associated with adverse outcomes in primary cutaneous melanoma (PCM). Detection of LVI by hematoxylin and eosin staining alone is 0%-6%, but targeting lymphovascular structures increases the detection rate. OBJECTIVE To examine the prognostic significance of LVI detected by immunostaining for D2-40 and microphthalmia-associated transcription factor 1 (MITF1) in PCM. METHODS The authors retrospectively analyzed 120 PCM samples. We compared the LVI detection rates of immunostaining for D2-40 only (22%), double staining for D2-40 and MITF1 (38%), and hematoxylin and eosin, and examined the association of LVI with clinicopathologic variables and clinical outcomes. RESULTS Immunolabeling with both methods significantly increased the LVI detection rate. Double staining for D2-40 and MITF1 as well as D2-40-detected LVI was significantly associated with increased Breslow thickness, number of mitoses, and sentinel lymph node (SLN) metastasis. D2-40-detected LVI was also associated with ulceration. Although the difference was not significant, double staining for D2-40 and MITF1 allowed for easier detection of LVI than D2-40 alone. LIMITATIONS This study was conducted in a tertiary referral institution; therefore, a referral bias cannot be excluded. CONCLUSIONS Immunolabeling increased detection of LVI in PCM. Because LVI is a positive predictive marker for SLN metastasis, the authors propose using anti-D2-40 and anti-MITF1 in the evaluation of LVI in patients with PCM with a certain risk of SLN metastasis.

Posterior Vertical Bite Reconstructions of Erosively Worn Dentitions and the "Stamp Technique" - A Case Series with a Mean Observation Time of 40 Months

PURPOSE In the present case series, the authors report on seven cases of erosively worn dentitions (98 posterior teeth) which were treated with direct resin composite. MATERIALS AND METHODS In all cases, both arches were restored by using the so-called stamp technique. All patients were treated with standardized materials and protocols. Prior to treatment, a waxup was made on die-cast models to build up the loss of occlusion as well as ensure the optimal future anatomy and function of the eroded teeth to be restored. During treatment, teeth were restored by using templates of silicone (ie, two "stamps," one on the vestibular, one on the oral aspect of each tooth), which were filled with resin composite in order to transfer the planned, future restoration (ie, in the shape of the waxup) from the extra- to the intraoral situation. Baseline examinations were performed in all patients after treatment, and photographs as well as radiographs were taken. To evaluate the outcome, the modified United States Public Health Service criteria (USPHS) were used. RESULTS The patients were re-assessed after a mean observation time of 40 months (40.8 ± 7.2 months). The overall outcome of the restorations was good, and almost exclusively "Alpha" scores were given. Only the marginal integrity and the anatomical form received a "Charlie" score (10.2%) in two cases. CONCLUSION Direct resin composite restorations made with the stamp technique are a valuable treatment option for restoring erosively worn dentitions.

Genesis 31, 39-40 und anderes : Herrn Professor Dr. I. Heinemann, dem Sechzigjährigen, in Freundschaft und Verehrung gewidmet

Harry Torczyner

Die Einbringung der Fahne des 40. (palästinensischen) Bataillons zu Chanuka 5686 nach Jerusalem [Illustration] : (Fotografie)

Scan von Monochrom-Mikroform

Ms. Barth. 116 (Ausst. 40) - Festmissale für St. Bartholomaeus

Vorbesitzer: Bartholomaeusstift Frankfurt am Main

Sieg der Reform und des Geistes. Abschiedspredigt über Jesaias 40,8 : "es dorret das Gras; es welket die Blume; doch unseres Gottes Wort besteht in Ewigkeit"

gehalten in der Hauptsynagoge zu Frankfurt a. M. am Trostsabbath 5622 (9. August 1862) von Leopold Stein

Ms. germ. oct. 40 - Reim-Apokalypse

Trägerband: Inc. oct. 28; Vorbesitzer: Dominikanerkloster Frankfurt am Main;

Ms. Praed. 40 - Epistolae [et tractatus]

Vorbesitzer: Dominikanerkloster Frankfurt am Main

[Signatur: W 1153 Nr. 40]

In Fraktur

Ms. lat. qu. 40 - Operum fragmenta quaedam

Vorbesitzer: Bernhard Waldschmidt

Ms.Ff.F.Stoltze 3. 40 - Brief von Margaretha Duck an Friedrich Stoltze und Marie Stoltze

Bitte um Unterstützung

Düngungsversuche des Deutschen Hopfenbauvereins mit Phonolithmehl (Kalisilikat) im Vergleich zu 40 % igem Kalisalz in den Jahren 1910-1912

Von Dr. J. Wagner

Düngungsversuche des Deutschen Hopfenbauvereins mit Phonolithmehl (Kalisilikat) im Vergleich zu 40 % igem Kalisalz in den Jahren 1910 - 1912

Von Dr. F. Wagner