(Table S2) Relative abundance of archaeal 16S rRNA genes in sediment cores

Deep drilling into the marine sea floor has uncovered a vast sedimentary ecosystem of microbial cells (Parkes et al., 1994, doi:10.1038/371410a0; D'Hondt et al., 2004, doi:10.1126/science.1101155). Extrapolation of direct counts of stained microbial cells to the total volume of habitable marine subsurface sediments suggests that between 56 Pg (Parkes et al., 1994, doi:10.1038/371410a0) and 303 Pg (Whitman et al., 1998) of cellular carbon could be stored in this largely unexplored habitat. From recent studies using various culture-independent techniques, no clear picture has yet emerged as to whether Archaea or Bacteria are more abundant in this extensive ecosystem (Schippers et al., doi:10.1038/nature03302; Inagaki et al., doi:10.1073/pnas.0511033103 ; Mauclaire et al., doi:10.1111/j.1472-4677.2004.00035.x; Biddle et al., doi:10.1073/pnas.0600035103). Here we show that in subsurface sediments buried deeper than 1 m in a wide range of oceanographic settings at least 87% of intact polar membrane lipids, biomarkers for the presence of live cells (Biddle et al., doi:10.1073/pnas.0600035103; Sturt et al., 2004, doi:10.1002/rcm.1378), are attributable to archaeal membranes, suggesting that Archaea constitute a major fraction of the biomass. Results obtained from modified quantitative polymerase chain reaction and slot-blot hybridization protocols support the lipid-based evidence and indicate that these techniques have previously underestimated archaeal biomass. The lipid concentrations are proportional to those of total organic carbon. On the basis of this relationship, we derived an independent estimate of amounts of cellular carbon in the global marine subsurface biosphere. Our estimate of 90 Pg of cellular carbon is consistent, within an order of magnitude, with previous estimates, and underscores the importance of marine subsurface habitats for global biomass budgets.

Pore water measurements of sediment cores from the Helgoland mud area, North Sea

Iron reduction in subseafloor sulfate-depleted and methane-rich marine sediments is currently a subject of interest in subsurface geomicrobiology. While iron reduction and microorganisms involved have been well studied in marine surface sediments, little is known about microorganisms responsible for iron reduction in deep methanic sediments. Here, we used quantitative PCR (Q-PCR)-based 16S rRNA gene copy numbers and pyrosequencing-based relative abundances of bacteria and archaea to investigate covariance between distinct microbial populations and specific geochemical profiles in the top 5 m of sediment cores from the Helgoland mud area, North Sea. We found that gene copy numbers of bacteria and archaea were specifically higher around the peak of dissolved iron in the methanic zone (250-350 cm. The higher copy numbers at these depths were also reflected by the relative sequence abundances of members of the candidate division JS1, methanogenic and Methanohalobium/ANME-3 related archaea. The distribution of these populations was strongly correlated to the profile of pore-water Fe2+ while that of Desulfobacteraceae corresponded to the pore-water sulfate profile. Furthermore, specific JS1 populations also strongly co-varied with the distribution of Methanosaetaceae in the methanic zone. Our data suggest that the interplay among JS1 bacteria, methanogenic archaea and Methanohalobium/ANME-3-related archaea may be important for iron reduction and methane cycling in deep methanic sediments of the Helgoland mud area and perhaps in other methane-rich depositional environments. .

(Table S2) The reconstructed sea surface temperature (SST) over the eastern equatorial Pacific

Abundant hydroclimatic evidence from western Amazonia and the adjacent Andes documents wet conditions during Heinrich Stadial 1 (HS1, 18-15 ka), a cold period in the high latitudes of the North Atlantic. This precipitation anomaly was attributed to a strengthening of the South American summer monsoon due to a change in the Atlantic interhemispheric sea surface temperature (SST) gradient. However, the physical viability of this mechanism has never been rigorously tested. We address this issue by combining a thorough compilation of tropical South American paleorecords and a set of atmosphere model sensitivity experiments. Our results show that the Atlantic SST variations alone, although leading to dry conditions in northern South America and wet conditions in northeastern Brazil, cannot produce increased precipitation over western Amazonia and the adjacent Andes during HS1. Instead, an eastern equatorial Pacific SST increase (i.e., 0.5-1.5 °C), in response to the slowdown of the Atlantic Meridional Overturning Circulation during HS1, is crucial to generate the wet conditions in these regions. The mechanism works via anomalous low sea level pressure over the eastern equatorial Pacific, which promotes a regional easterly low-level wind anomaly and moisture recycling from central Amazonia towards the Andes.

Hs. Rothschild 4° 115 - Testament des Bernhard Juda Sichel und seiner Ehegattin Betty aus dem Jahr 1858 (Abschrift)

Vorbesitzer: Freiherrlich Carl von Rothschild'sche Bibliothek Frankfurt am Main; alte Signatur: Hs. in Quart 115

Hs. Rothschild 4° 104 - Viertes Testament des Mayer Amschel Rothschild aus dem Jahr 1812 (Abschrift)

Vorbesitzer: Freiherrlich Carl von Rothschild'sche Bibliothek Frankfurt am Main; alte Signatur: Hs. in Quart 104; Hs in 4° 104

Hs. Rothschild 4° 105 - Viertes Testament des Mayer Amschel Rothschild aus dem Jahr 1812 (Abschrift)

Vorbesitzer: Freiherrlich Carl von Rothschild'sche Bibliothek Frankfurt am Main; alte Signatur: Hs. in Quart 105; Hs in 4° 105

Hs. Rothschild 4° 108 - Testament der Jeanette Worms geborene Rothschild mit Nachtrag und Codicillen aus den Jahren 1829 - 1859 (Abschriften)

Vorbesitzer: Freiherrlich Carl von Rothschild'sche Bibliothek Frankfurt am Main; alte Signatur: Hs. in Quart 108

Hs. Rothschild 4° 114 - Testament des Bernhard Juda Sichel und seiner Ehegattin Betty aus dem Jahr 1858 (Abschrift)

Vorbesitzer: Freiherrlich Carl von Rothschild'sche Bibliothek Frankfurt am Main; alte Signatur: Hs. in Quart 114

Hs. Rothschild 4° 109 - Testament der Jeanette Worms geborene Rothschild mit Nachtrag und Codicillen aus den Jahren 1829 - 1859 (Abschriften)

Vorbesitzer: Freiherrlich Carl von Rothschild'sche Bibliothek Frankfurt am Main; alte Signatur: Hs. in Quart 109

Hs. Rothschild 8° 57 - 35 Briefe der Freiin Louise von Rothschild und ihrer Tochter Hannah Louise von Rothschild an den Buchhändler Moritz Abendroth aus den Jahren 1875 - 1889

Vorbesitzer: Moritz Abendroth; Fräulein Abendroth; Freiherrlich Carl von Rothschild'sche öffentliche Bibliothek; alte Signatur: Hs. in Oktav 57; Hs in 8° 57

Evaluation of the zinox and zeolite materials as adsorbents to remove H2S from natural gas

MELO, Dulce Maria de Araújo et al. Evaluation of the Zinox and Zeolite materials as adsorbents to remove H2S from natural gas. Colloids and Surfaces. A, Physicochemical and Engineering Aspects, Estados Unidos, v. 272, p. 32-36, 2006.