CHARACTERIZATION OF THE COUNT RATE PERFORMANCE AND EVALUATION OF THE EFFECTS OF HIGH COUNT RATES ON MODERN GAMMA CAMERAS

CHARACTERIZATION OF THE COUNT RATE PERFORMANCE AND EVALUATION OF THE EFFECTS OF HIGH COUNT RATES ON MODERN GAMMA CAMERAS Michael Stephen Silosky, B.S. Supervisory Professor: S. Cheenu Kappadath, Ph.D. Evaluation of count rate performance (CRP) is an integral component of gamma camera quality assurance and measurement of system dead time (τ) is important for quantitative SPECT. The CRP of three modern gamma cameras was characterized using established methods (Decay and Dual Source) under a variety of experimental conditions. For the Decay method, input count rate was plotted against observed count rate and fit to the paralyzable detector model (PDM) to estimate τ (Rates method). A novel expression for observed counts as a function of measurement time interval was derived and the observed counts were fit to this expression to estimate τ (Counts method). Correlation and Bland-Altman analysis were performed to assess agreement in estimates of τ between methods. The dependencies of τ on energy window definition and incident energy spectrum were characterized. The Dual Source method was also used to estimate τ and its agreement with the Decay method under identical conditions and the effects of total activity and the ratio of source activities were investigated. Additionally, the effects of count rate on several performance metrics were evaluated. The CRP curves for each system agreed with the PDM at low count rates but deviated substantially at high count rates. Estimates of τ for the paralyzable portion of the CRP curves using the Rates and Counts methods were highly correlated (r=0.999) but with a small (~6%) difference. No significant difference was observed between the highly correlated estimates of τ using the Decay or Dual Source methods under identical experimental conditions (r=0.996). Estimates of τ increased as a power-law function with decreasing ratio of counts in the photopeak to the total counts and linearly with decreasing spectral effective energy. Dual Source method estimates of τ varied as a quadratic with the ratio of the single source to combined source activities and linearly with total activity used across a large range. Image uniformity, spatial resolution, and energy resolution degraded linearly with count rate and image distorting effects were observed. Guidelines for CRP testing and a possible method for the correction of count rate losses for clinical images have been proposed.

Cambios en la distribución minorista de alimentos y bebidas en Argentina 2001-2003 : Hacia nuevas horizontalidades territoriales

Se presentan en este trabajo los principales cambios en la distribución minorista de alimentos y bebidas en la Argentina iniciados con la crisis económica, política y social del año 2001. El territorio usado por parte de los actores sociales involucrados, como las empresas de diferentes tamaños (desde hipermercados a almacenes), y las personas (consumidores), ya no solo responderá a un orden global y a verticalidades territoriales, sino que muchas de estas nuevas tendencias estarán comandadas por normas locales relacionadas con la horizontalidad territorial. Entre estos cambios, son los más importantes: el consumo orientado a las ofertas; a empaques más chicos; a segundas y terceras marcas; a marcas propias de supermercados; a una nueva frecuencia en la realización de las compras; a la combinación de varios canales de distribución con el fenómeno denominado "vuelta al barrio", y a compras de productos sueltos.

Cambios en la distribución minorista de alimentos y bebidas en Argentina 2001-2003 : Hacia nuevas horizontalidades territoriales

Se presentan en este trabajo los principales cambios en la distribución minorista de alimentos y bebidas en la Argentina iniciados con la crisis económica, política y social del año 2001. El territorio usado por parte de los actores sociales involucrados, como las empresas de diferentes tamaños (desde hipermercados a almacenes), y las personas (consumidores), ya no solo responderá a un orden global y a verticalidades territoriales, sino que muchas de estas nuevas tendencias estarán comandadas por normas locales relacionadas con la horizontalidad territorial. Entre estos cambios, son los más importantes: el consumo orientado a las ofertas; a empaques más chicos; a segundas y terceras marcas; a marcas propias de supermercados; a una nueva frecuencia en la realización de las compras; a la combinación de varios canales de distribución con el fenómeno denominado "vuelta al barrio", y a compras de productos sueltos.

Cambios en la distribución minorista de alimentos y bebidas en Argentina 2001-2003 : Hacia nuevas horizontalidades territoriales

Se presentan en este trabajo los principales cambios en la distribución minorista de alimentos y bebidas en la Argentina iniciados con la crisis económica, política y social del año 2001. El territorio usado por parte de los actores sociales involucrados, como las empresas de diferentes tamaños (desde hipermercados a almacenes), y las personas (consumidores), ya no solo responderá a un orden global y a verticalidades territoriales, sino que muchas de estas nuevas tendencias estarán comandadas por normas locales relacionadas con la horizontalidad territorial. Entre estos cambios, son los más importantes: el consumo orientado a las ofertas; a empaques más chicos; a segundas y terceras marcas; a marcas propias de supermercados; a una nueva frecuencia en la realización de las compras; a la combinación de varios canales de distribución con el fenómeno denominado "vuelta al barrio", y a compras de productos sueltos.

Berry production, shoot growth and biomass of E. hermaphroditum, V. vitis-idaea and V. myrtillis at Abisco ANS

Extreme weather events can have strong negative impacts on species survival and community structure when surpassing lethal thresholds. Extreme, short-lived, winter warming events in the Arctic rapidly melt snow and expose ecosystems to unseasonably warm air (for instance, 2-10 °C for 2-14 days) but upon return to normal winter climate exposes the ecosystem to much colder temperatures due to the loss of insulating snow. Single events have been shown to reduce plant reproduction and increase shoot mortality, but impacts of multiple events are little understood as are the broader impacts on community structure, growth, carbon balance, and nutrient cycling. To address these issues, we simulated week-long extreme winter warming events - using infrared heating lamps and soil warming cables - for 3 consecutive years in a sub-Arctic heathland dominated by the dwarf shrubs Empetrum hermaphroditum, Vaccinium vitis-idaea (both evergreen) and Vaccinium myrtillus (deciduous). During the growing seasons after the second and third winter event, spring bud burst was delayed by up to a week for E. hermaphroditum and V. myrtillus, and berry production reduced by 11-75% and 52-95% for E. hermaphroditum and V. myrtillus, respectively. Greater shoot mortality occurred in E. hermaphroditum (up to 52%), V. vitis-idaea (51%), and V. myrtillus (80%). Root growth was reduced by more than 25% but soil nutrient availability remained unaffected. Gross primary productivity was reduced by more than 50% in the summer following the third simulation. Overall, the extent of damage was considerable, and critically plant responses were opposite in direction to the increased growth seen in long-term summer warming simulations and the 'greening' seen for some arctic regions. Given the Arctic is warming more in winter than summer, and extreme events are predicted to become more frequent, this generates large uncertainty in our current understanding of arctic ecosystem responses to climate change.

Foreign Direct Investment Relations between Myanmar and ASEAN

Myanmar highly appreciates foreign direct investment (FDI) as a key solution reducing the development gap with leading ASEAN countries. Accordingly, it is welcomed by the government. Myanmar's Foreign Investment Law was enacted in 1988 soon after the adoption of a market-oriented economic system to boost the flow of FDI into the country. Foreign investors positively responded to these measures in the early years and FDI inflow into Myanmar gradually increased during the period from 1989 to 1996. However, after 1997, FDI inflow was dramatically reduced and markedly declined until 2004. In 2005, FDI inflow increased at an unprecedented rate and reached the highest level in the country's history. However, this growth was not sustainable in the subsequent years, as it declined again and turned stagnant at the previous level. In terms of source regions, ASEAN is a major investor in Myanmar, which investment is significantly exceeds the combined investment of other regions of the world. Among top ten countries, Thailand's investment alone is significantly more than combined total investments of the other nine countries. Next to Thailand in terms of investments in Myanmar are Singapore and Malaysia among ASEAN, at second and third places, respectively. The combined total FDI inflows into the power and oil and gas sector represent about 65 percent of the total investment. There are many opportunities for foreign investment in other sectors, which are not, yet exploited. ASEAN countries will certainly be source countries of Myanmar FDI in the future, and Myanmar should expand to other Asian countries like Japan, India, China, Korea, and Hong Kong where its FDI portfolio is concerned. To effectively attract FDI into the country, Myanmar needs to minimize the effect of policy while opening and encouraging other potential sectors of FDI to foreign investors in ASEAN and Asian countries.

A syntactic approach to combining functional notation, lazy evaluation, and higher-order in LP systems

Nondeterminism and partially instantiated data structures give logic programming expressive power beyond that of functional programming. However, functional programming often provides convenient syntactic features, such as having a designated implicit output argument, which allow function cali nesting and sometimes results in more compact code. Functional programming also sometimes allows a more direct encoding of lazy evaluation, with its ability to deal with infinite data structures. We present a syntactic functional extensión, used in the Ciao system, which can be implemented in ISO-standard Prolog systems and covers function application, predefined evaluable functors, functional definitions, quoting, and lazy evaluation. The extensión is also composable with higher-order features and can be combined with other extensions to ISO-Prolog such as constraints. We also highlight the features of the Ciao system which help implementation and present some data on the overhead of using lazy evaluation with respect to eager evaluation.

Silencing microRNA-134 produces neuroprotective and prolonged seizure-suppressive effects

Temporal lobe epilepsy is a common, chronic neurological disorder characterized by recurrent spontaneous seizures. MicroRNAs (miRNAs) are small, noncoding RNAs that regulate post-transcriptional expression of protein-coding mRNAs, which may have key roles in the pathogenesis of neurological disorders. In experimental models of prolonged, injurious seizures (status epilepticus) and in human epilepsy, we found upregulation of miR-134, a brain-specific, activity-regulated miRNA that has been implicated in the control of dendritic spine morphology. Silencing of miR-134 expression in vivo using antagomirs reduced hippocampal CA3 pyramidal neuron dendrite spine density by 21% and rendered mice refractory to seizures and hippocampal injury caused by status epilepticus. Depletion of miR-134 after status epilepticus in mice reduced the later occurrence of spontaneous seizures by over 90% and mitigated the attendant pathological features of temporal lobe epilepsy. Thus, silencing miR-134 exerts prolonged seizure-suppressant and neuroprotective actions; determining whether these are anticonvulsant effects or are truly antiepileptogenic effects requires additional experimentation.

Dynamic gamma frequency feedback coupling between higher and lower order visual cortices underlies perceptual completion in humans

To perceive a coherent environment, incomplete or overlapping visual forms must be integrated into meaningful coherent percepts, a process referred to as ?Gestalt? formation or perceptual completion. Increasing evidence suggests that this process engages oscillatory neuronal activity in a distributed neuronal assembly. A separate line of evidence suggests that Gestalt formation requires top-down feedback from higher order brain regions to early visual cortex. Here we combine magnetoencephalography (MEG) and effective connectivity analysis in the frequency domain to specifically address the effective coupling between sources of oscillatory brain activity during Gestalt formation. We demonstrate that perceptual completion of two-tone ?Mooney? faces induces increased gamma frequency band power (55?71 Hz) in human early visual, fusiform and parietal cortices. Within this distributed neuronal assembly fusiform and parietal gamma oscillators are coupled by forward and backward connectivity during Mooney face perception, indicating reciprocal influences of gamma activity between these higher order visual brain regions. Critically, gamma band oscillations in early visual cortex are modulated by top-down feedback connectivity from both fusiform and parietal cortices. Thus, we provide a mechanistic account of Gestalt perception in which gamma oscillations in feature sensitive and spatial attention-relevant brain regions reciprocally drive one another and convey global stimulus aspects to local processing units at low levels of the sensory hierarchy by top-down feedback. Our data therefore support the notion of inverse hierarchical processing within the visual system underlying awareness of coherent percepts.

Climate and grazing control nurse effects in an Ecuadorian dry shrubby community

Positive plant interactions have strong effects on plant diversity at several spatial scales, expanding species distribution under stressful conditions. We evaluated the joint effect of climate and grazing on the nurse effect of Croton wagneri, by monitoring several community attributes at two spatial scales: microhabitat and plant community. Two very close locations that only differed in grazing intensity were surveyed in an Ecuadorian dry scrub ecosystem. At each location, two 30 × 30-m plots were established at four altitudinal levels (1500, 2630, 1959 and 2100 m asl) and 40 microsites were surveyed in each plot. Croton wagneri acted as community hubs, increasing species richness and plant cover at both scales. Beneath nurses mean richness and cover values were 3.4 and 21.9%, and in open areas 2.3 and 4.5%, respectively. Magnitude of nurse effect was dependent on climate and grazing conditions. In ungrazed locations, cover increased and diversity reduced with altitude, while grazed locations showed the opposite trend. In ungrazed plots the interactions shifted from positive to negative with altitude, in grazed locations interactions remained positive. We conclude that the nurse effect is a key mechanism regulating community properties not only at microsite but also at the entire community scale.

Innovación educativa en las redes sociales online: un estudio en la educación superior

Las redes sociales son tan antiguas como las relaciones entre los humanos, ya que las labores de cooperación siempre fueron imprescindibles en los procesos de asociación, tanto los formados por simples células hasta los constituidos por seres vivos. La sociedad está formada por una gran cantidad de situaciones que fomentan dicha colaboración. Desde la creación del Espacio Europeo de Educación Superior se ha promovido la utilización de entornos virtuales de aprendizaje que favorezcan una evaluación más transparente y objetiva. Las redes sociales online son las interfaces digitales más utilizadas por los estudiantes universitarios, por lo que son muchos los expertos que recomiendan analizar la utilidad educativa de este tipo de aplicaciones. Siguiendo estas recomendaciones se implementó una red social online, llamada Formatio, que se utilizó para apoyar el proceso de enseñanza y aprendizaje en la educación superior. El objetivo general de esta tesis doctoral fue valorar el nivel de aceptación de este entorno virtual de aprendizaje para los estudiantes universitarios que participaron en este estudio. Para evaluar el uso de la plataforma se analizaron las actividades generadas por los 172 estudiantes, seleccionados aleatoriamente, matriculados en la asignatura de Estadística y TIC en el curso 2010/2011, del Grado en Ciencias de la Actividad Física y el Deporte. Con el objetivo de estimar la utilidad educativa de las redes sociales online se diseñó y validó el instrumento de Valoración de Recursos Educativos Online (VREO), que fue aplicado a otra muestra aleatoria de 452 estudiantes de la misma facultad durante el curso 2011/2012. Los resultados confirman el éxito de este tipo de espacios virtuales gracias a la alta frecuencia de uso conseguida y a las buenas puntuaciones obtenidas por los alumnos, quienes tienen una buena opinión sobre las ventajas de usar las redes sociales online en la educación superior. De todos los recursos analizados la red social online Formatio ha sido la opción mejor valorada, seguida de Moodle y Facebook en segundo y tercer lugar, y de Twitter y YouTube, en cuarta y en quinta posición. Este estudio establece un punto de partida para futuras investigaciones encaminadas a obtener ciertos indicadores cualitativos y cuantitativos que faciliten una evaluación más justa de los actores integrantes de los sistemas educativos, que ayude a abrir las puertas de nuestra sociedad a la responsabilidad, la eficiencia, la transparencia y la objetividad. ABSTRACT Social networks are as old as human relations, since cooperative work has always been indispensable in all processes of association, from those composed of simple cells to those formed by living beings. Society is shaped by a large amount of situations that stimulate this cooperation. Since the creation of the European Higher Education Area, the use of virtual learning environments that favor more transparent and objective evaluation has been promoted. On-line social networks are the digital interfaces most widely used by university students, which is the reason why many experts recommend an analysis of the educational utility of this type of applications. Following these recommendations, an on-line social network called Formatio was created, which was used in order to support the teaching and learning process in higher education. The general objective of this doctoral dissertation was to evaluate the level of acceptance of this virtual learning environment by the university students who participated in this study. In order to evaluate the use of the platform, the activity created by 172 students chosen at random was analyzed; they were registered in the course on Statistics and Information and Communication Technology of the degree program in Sciences of Physical Activity and Sport during the academic year 2010/2011. With the goal of appraising the educational utility of on-line social networks, a tool for Evaluation of On-line Educational Resources (EOER) was designed, validated, and applied to another random sample of 452 students from the same department during the academic year 2011/2012. The results confirm the success of this type of virtual spaces, thanks to the high frequency of use achieved and the good grades obtained by the students who had a favorable opinion of the advantages of using on-line social networks in higher education. Out of all the resources analyzed, the Formatio on-line social network was the option that received the best evaluation, followed by Moodle and Facebook, which were second and third, and Twitter and YouTube, in fourth and fifth place respectively. This study establishes a starting point for future research that aims to obtain certain qualitative and quantitative indicators that will make it easier to engage in a fairer evaluation of the parties involved in education systems, which will help open the doors of our society to responsibility, efficiency, transparency, and objectivity.

Direct adenovirus-mediated gene transfer of interleukin 1 and tumor necrosis factor α soluble receptors to rabbit knees with experimental arthritis has local and distal anti-arthritic effects

Adenoviral vectors were used to deliver genes encoding a soluble interleukin 1 (IL-1)-type I receptor-IgG fusion protein and/or a soluble type I tumor necrosis factor α (TNFα) receptor-IgG fusion protein directly to the knees of rabbits with antigen-induced arthritis. When tested individually, knees receiving the soluble IL-1 receptor had significantly reduced cartilage matrix degradation and white blood cell infiltration into the joint space. Delivery of the soluble TNFα receptor was less effective, having only a moderate effect on white blood cell infiltration and no effect on cartilage breakdown. When both soluble receptors were used together, there was a greater inhibition of white blood cell infiltration and cartilage breakdown with a considerable reduction of synovitis. Interestingly, anti-arthritic effects were also seen in contralateral control knees receiving only a marker gene, suggesting that sustained local inhibition of disease activity in one joint may confer an anti-arthritic effect on other joints. These results suggest that local intra-articular gene transfer could be used to treat systemic polyarticular arthritides.

Nck-2, a Novel Src Homology2/3-containing Adaptor Protein That Interacts with the LIM-only Protein PINCH and Components of Growth Factor Receptor Kinase-signaling Pathways

Many of the protein–protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. Nck is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. We report here the identification, cloning, and characterization of a widely expressed, Nck-related adaptor protein termed Nck-2. Nck-2 comprises primarily three N-terminal SH3 domains and one C-terminal SH2 domain. We show that Nck-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling. The PINCH-Nck-2 interaction is mediated by the fourth LIM domain of PINCH and the third SH3 domain of Nck-2. Furthermore, we show that Nck-2 is capable of recognizing several key components of growth factor receptor kinase-signaling pathways including EGF receptors, PDGF receptor-β, and IRS-1. The association of Nck-2 with EGF receptors was regulated by EGF stimulation and involved largely the SH2 domain of Nck-2, although the SH3 domains of Nck-2 also contributed to the complex formation. The association of Nck-2 with PDGF receptor-β was dependent on PDGF activation and was mediated solely by the SH2 domain of Nck-2. Additionally, we have detected a stable association between Nck-2 and IRS-1 that was mediated primarily via the second and third SH3 domain of Nck-2. Thus, Nck-2 associates with PINCH and components of different growth factor receptor-signaling pathways via distinct mechanisms. Finally, we provide evidence indicating that a fraction of the Nck-2 and/or Nck-1 proteins are associated with the cytoskeleton. These results identify a novel Nck-related SH2- and SH3-domain–containing protein and suggest that it may function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways.