Evaluating plant breeding strategies by simulating gene action and dryland environment effects

Functional genomics is the systematic study of genome-wide effects of gene expression on organism growth and development with the ultimate aim of understanding how networks of genes influence traits. Here, we use a dynamic biophysical cropping systems model (APSIM-Sorg) to generate a state space of genotype performance based on 15 genes controlling four adaptive traits and then search this spice using a quantitative genetics model of a plant breeding program (QU-GENE) to simulate recurrent selection. Complex epistatic and gene X environment effects were generated for yield even though gene action at the trait level had been defined as simple additive effects. Given alternative breeding strategies that restricted either the cultivar maturity type or the drought environment type, the positive (+) alleles for 15 genes associated with the four adaptive traits were accumulated at different rates over cycles of selection. While early maturing genotypes were favored in the Severe-Terminal drought environment type, late genotypes were favored in the Mild-Terminal and Midseason drought environment types. In the Severe-Terminal environment, there was an interaction of the stay-green (SG) trait with other traits: Selection for + alleles of the SG genes was delayed until + alleles for genes associated with the transpiration efficiency and osmotic adjustment traits had been fixed. Given limitations in our current understanding of trait interaction and genetic control, the results are not conclusive. However, they demonstrate how the per se complexity of gene X gene X environment interactions will challenge the application of genomics and marker-assisted selection in crop improvement for dryland adaptation.

Sensitivity of Helicoverpa armigera nucleopolyhedrovirus polyhedra to sodium dodecyl sulfate

Sodium dodecyl sulfate (SDS) is commonly used to extract polyhedra from infected cells and diseased dead larval tissues. It was found, however, that 80% of Helicoverpa armigera nucleopolyhedrovirus (HaSNPV) polyhedra produced via cell culture were damaged after 30 min of 0.5% SDS treatment whereas only 20% of in vivo produced polyhedra were damaged by the same treatment. Transmission and scanning electron microscopy revealed that the damaged polyhedra had lost their polyhedron envelopes and virions were dislodged from the polyhedrin matrix, leaving empty spaces that were previously occupied by the occluded virions. Up to 20% in vitro produced polyhedra were resistant to SDS and remained intact, even after a 24 h exposure to SDS. This sensitivity to SDS was observed across a range of cell culture media, including serum supplemented media. Electron microscopy also revealed that the inferior polyhedron envelope of in vitro produced polyhedra is likely due to poor interaction between the polyhedron envelope, polyhedron envelope protein (PEP), and polyhedrin matrix. The PEP gene was cloned and sequenced and mutations in this gene were ruled out as an explanation. In vitro produced polyhedra that were passed through insect larva once were resistant to SDS, indicating that a critical component is lacking in insect cell culture medium used for producing HaSNPV or the cells growing in culture are inefficient in some ways in relation to production of polyhedra. (C) 2002 Elsevier Science (USA). All rights reserved.

Recombinant expression of Munc18c in a baculovirus system and interaction with syntaxin4

Two protein families that are critical for vesicle transport are the Syntaxin and Munc18/Sec1. families of proteins. These two molecules form a high affinity complex and play an essential role in vesicle docking and fusion. Munc18c was expressed as an N-terminally His-tagged fusion protein from recombinant baculovirus in Sf9 insect cells. His-tagged Munc18c was purified to homogeneity using both cobalt-chelating affinity chromatography and gel filtration chromatography. With this simple two-step protocol, 3.5 mg of purified Munc18c was obtained from a 1 L culture. Further, the N-terminal His-tag could be removed by thrombin cleavage while the tagged protein was bound to metal affinity resin. Recombinant Munc18c produced in this way is functional, in that it forms a stable complex with the SNARE interacting partner, syntaxin4. Thus we have developed a method for producing and purifying large amounts of functional Munc18c-both tagged and detagged-from a baculovirus expression system. We have also developed a method to purify the Munc18c:syntaxin4 complex. These methods will be employed for future functional and structural studies. Crown copyright (C) 2003 Published by Elsevier Inc. All rights reserved.

A yield architecture framework to explain adaptation of pearl millet to environmental stress

Functional knowledge of the physiological basis of crop adaptation to stress is a prerequisite for exploiting specific adaptation to stress environments in breeding programs. This paper presents an analysis of yield components for pearl millet, to explain the specific adaptation of local landraces to stress environments in Rajasthan, India. Six genotypes, ranging from high-tillering traditional landraces to low-tillering open-pollinated modern cultivars, were grown in 20 experiments, covering a range of nonstress and drought stress patterns. In each experiment, yield components (particle number, grain number, 100 grain mass) were measured separately for main shoots, basal tillers, and nodal tillers. Under optimum conditions, landraces had a significantly lower grain yield than the cultivars, but no significant differences were observed at yield levels around 1 ton ha(-1). This genotype x environment interaction for grain yield was due to a difference in yield strategy, where landraces aimed at minimising the risk of a crop failure under stress conditions, and modem cultivars aimed at maximising yield potential under optimum conditions. A key aspect of the adaptation of landraces was the small size of the main shoot panicle, as it minimised (1) the loss of productive tillers during stem elongation; (2) the delay in anthesis if mid-season drought occurs; and (3) the reduction in panicle productivity of the basal tillers under stress. In addition, a low investment in structural panicle weight, relative to vegetative crop growth rate, promoted the production of nodal tillers, providing a mechanism to compensate for reduced basal tiller productivity if stress occurred around anthesis. A low maximum 100 grain mass also ensured individual grain mass was little affected by environmental conditions. The strategy of the high-tillering landraces carries a yield penalty under optimum conditions, but is expected to minimise the risk of a crop failure, particularly if mid-season drought stress occurs. The yield architecture of low-tillering varieties, by contrast, will be suited to end-of-season drought stress, provided anthesis is early. Application of the above adaptation mechanisms into a breeding program could enable the identification of plant types that match the prevalent stress patterns in the target environments. (C) 2003 E.J. van Oosterom. Published by Elsevier Science B.V. All rights reserved.

Towards Emotional Interaction: Using Movies to Automatically Learn Users' Emotional States

The HCI community is actively seeking novel methodologies to gain insight into the user’s experience during interaction with both the application and the content. We propose an emotional recognition engine capable of automatically recognizing a set of human emotional states using psychophysiological measures of the autonomous nervous system, including galvanic skin response, respiration, and heart rate. A novel pattern recognition system, based on discriminant analysis and support vector machine classifiers is trained using movies’ scenes selected to induce emotions ranging from the positive to the negative valence dimension, including happiness, anger, disgust, sadness, and fear. In this paper we introduce an emotion recognition system and evaluate its accuracy by presenting the results of an experiment conducted with three physiologic sensors.

Da partilha à acção colectiva dos cidadãos: O contributo dos Novos Media no apelo à dádiva de medula óssea

O contributo das redes sociais na luta contra o cancro tem sido conhecida, especialmente, pelo sucesso de movimentos de cidadãos com o objectivo de sensibilizar a sociedade portuguesa para a dádiva de medula óssea. Este tipo de campanhas solidárias, com o apoio dos Novos Media, foi um fenómeno iniciado em 2009 e tem vindo a desenvolver-se, sobretudo, no Facebook. Desde então, novos movimentos têm aparecido regularmente nesta rede social e ajudado a um aumento significativo do registo de dadores. Interessa-nos perceber como se desenvolvem este tipo de campanhas e que factores, contribuíram para o seu sucesso, procurando averiguar não só o poder da Web 2.0, mas também questões sobre a comunicação, a imagem e os media. O sucesso destes movimentos vai muito além da simples presença no Facebook. A análise global do sistema de comunicação é fundamental para compreender o diferente nível de sucesso atingido por cada movimento. No presente artigo, fazemos uma análise comparativa entre os principais movimentos de cidadãos, ocorridos entre 2009 e 2011, que comprova as supracitadas hipóteses.

Os Media Participativos e o poder da Identidade na Oncologia: o Cidadão e a Instituição enquanto Marcas interactivas

Neste artigo procuramos averiguar a relevância que as marcas e identidades têm nas plataformas participativas no contexto da saúde, em particular na oncologia. Apesar de estas plataformas disponibilizarem aos cidadãos e instituições as mesmas ferramentas, a sua capacidade de mobilização não é a mesma. As instituições parecem ter maior facilidade, mas nem sempre tal acontece. Discutir o conceito de marca e de identidade contribuirá para responder a esta questão, bem como compreender porque é que as diferenças entre os cidadãos e as instituições se podem atenuar nos novos media. Baseamos a nossa análise na observação de páginas do Facebook, de instituições oncológicas e de grupos de apoio de cidadãos; num conjunto de entrevistas a doentes e familiares oncológicos; e em diferentes estudos na saúde. No fim, procura-se esclarecer a importância do estudo das marcas e da identidade, como possível directriz de novas soluções nos media participativos, que contribuam para atenuar o problema individual do cidadão que se relaciona com o cancro.

OncologiaPediatrica.org e TalkingAboutCancer.org: A importância dos media participativos na mobilização para a cidadania na luta contra o cancro

O presente estudo tem como objectivo averiguar a validade do uso das plataformas participativas online em acções de cidadania que contribuam na luta contra o cancro. Estas plataformas são frequentemente associadas ao entretenimento; nesse sentido, pretende-se não só testar a supracitada aplicação a outros domínios como o da saúde, mas de igual forma contribuir para uma percepção expandida do potencial destas junto do seu universo de utilizadores. A investigação baseia-se na construção de uma rede solidária online que contribua para a aproximação de cidadãos que estão directa ou indirectamente envolvidos na problemática da oncologia, disponibilizando ao indivíduo meios que o ajudem a contrariar sentimentos de impotência e fatalidade e a ausência de capacidade de mobilização e de cidadania. No âmbito deste percurso de investigação, foi desenvolvida e implementada a plataforma OncologiaPediatrica.org, que teve como primeira missão confirmar a hipótese de o Design Multimédia poder contribuir para a informação, apoio e partilha de testemunhos entre familiares, amigos e doentes na luta contra o cancro pediátrico. Esta plataforma baseia-se num sistema centralizado, ou seja, a organização, política e gestão dos conteúdos informativos do site respeitam uma estrutura hierárquica piramidal. Os resultados do site OncologiaPediatrica.org e os desenvolvimentos tecnológicos conducentes ao florescimento das redes sociais e Web 2.0, entretanto ocorridos, exigiram um reequacionamento da abordagem anteriormente adoptada, por forma a tornar o projecto mais eficaz perante as realidades entretanto surgidas. Neste sentido, foi desenvolvido um protótipo, disponível em TalkingAboutCancer.org, mais descentralizado e aberto à participação da comunidade. O actual estudo conta com o apoio do Instituto Português de Oncologia do Porto. Presentemente averigua-se, junto dos doentes e profissionais de saúde deste hospital, de que forma os media participativos podem ajudar os cidadãos na luta contra o cancro. Os primeiros resultados da investigação são apresentados no presente artigo.

Os Media Participativos e a importância do Cidadão e da Instituição enquanto Marcas mobilizadoras na área da Oncologia

Present paper aims at surveying the relevance that brands and identities may have in participatory platforms in the context of oncology. Despite the fact that these platforms provide the same tools to citizens and institutions alike, their capacity for mobilization is distinct. In general, institutions seem to be more successful, although this is not always the case. Discussing the concept of brand and identity, as well as understanding how the differences between citizens and institutions may blur within the context of new media, may contribute to answering the above question. We anchor our analysis on the study of social network Facebook, oncological institutions and citizen support groups, and various health studies. The ultimate goal will be the clarification of the importance of brands and identity as a contributive factor towards new solutions in participatory media

The host-tourist interaction in a world heritage site: the case of Guimarães

Guimarães, in the northwest of Portugal, is a city of strong symbolic and cultural significance and its nomination by UNESCO as world heritage, in 2001, enlarged its tourism potential. In this paper we present a few results of a survey that envisaged capturing the Guimarães residents’ perceptions of tourism impacts and their attitudes towards tourists. Specifically, one analyzes the type of relationship that exists between some socio-demographic groups and the perceived tourism impacts, as well as their socio-characteristics and the existing level of interaction between residents and tourists. The survey was implemented between January and March 2010 to a convenience sample of 540 inhabitants of the municipality of Guimarães resulting in 400 questionnaires with complete data. For this, we made use of various statistical techniques. Using a factorial analysis, we can conclude that the three factors used explain 52.3% of the variance contained in the original variables obtained from the survey. By another side, using a logit model in the analysis and taking as the dependent variable the frequent or very frequent contact with tourists, we found that only the variables referred to perceived positive impacts of tourism, education and the place of residence in urban areas have shown to be statistically significant. We are aware of the multiple ways the issue of residents’ perceptions and attitudes towards tourism can be approached and of the difficulties to get useful policy-oriented insights. This paper is a step in that trail.

Avaliação ex-ante, pelos media, de Guimarães 2012 capital europeia da Cultura

A nomeação de Guimarães como Capital Europeia da Cultura (CEC) em 2012 veio colocar na ordem do dia a necessidade de medir os efeitos que a concretização deste megaevento irá provocar na cidade e no município. A conveniente ponderação dos benefícios e dos custos e um alargado envolvimento da comunidade tendem a permitir encontrar formas de reduzir os impactos negativos e aumentar os impactos positivos. A presente comunicação analisa o envolvimento da população e das associações locais no planeamento e organização da Capital Europeia da Cultura 2012, aproximados pela via da cobertura feita pela imprensa local e nacional à preparação do megaevento. A análise de conteúdo das notícias publicadas abrangeu o período que medeia entre Janeiro e Dezembro de 2011 e respeitou a dois semanários locais e um diário nacional. Com base na análise efetuada, pode concluir-se que foi fraco o envolvimento da população e dos agentes culturais na organização da CEC 2012 e que existiu uma forte reação negativa ao modelo de planeamento do megaevento conduzido pela estrutura organizadora oficial, o que veio pôr em causa a desejável adesão dos vimaranenses ao mesmo e, por essa via, o respetivo sucesso, sobretudo numa perspetiva de efeitos a médio e longo prazo.

Methods to classify maize cultivars in use efficiency and response to nitrogen

n plant breeding programs that aim to obtain cultivars with nitrogen (N) use efficiency, the focus is on methods of selection and experimental procedures that present low cost, fast response, high repeatability, and can be applied to a large number of cultivars. Thus, the objectives of this study were to classify maize cultivars regarding their use efficiency and response to N in a breeding program, and to validate the methodology with contrasting doses of the nutrient. The experimental design was a randomized block with the treatments arranged in a split-plot scheme with three replicates and five N doses (0, 30, 60, 120 and 200 kg ha-1) in the plots, and six cultivars in subplots. We compared a method examining the efficiency and response (ER) with two contrasting doses of N. After that, the analysis of variance, mean comparison and regression analysis were performed. In conclusion, the method of the use efficiency and response based on two N levels classifies the cultivars in the same way as the regression analysis, and it is appropriate in plant breeding routine. Thus, it is necessary to identify the levels of N required to discriminate maize cultivars in conditions of low and high N availability in plant breeding programs that aim to obtain efficient and responsive cultivars. Moreover, the analysis of the interaction genotype x environment at experiments with contrasting doses is always required, even when the interaction is not significant.

Ex-ante evaluation by the media of the Guimarães 2012 European Capital of Culture

The nomination of Guimarães to host the 2012 European Capital of Culture (ECC) has put on the agenda of the city the need of measuring the effects that the implementation of this mega event could have in it and in the municipality a whole. The balance of the benefits and costs and an extended community involvement tend to reduce negative impacts and enhance positive ones. This chapter analyzes the involvement of population and local associations in the planning and organization of the 2012 Guimarães European Capital of Culture, using the coverage made during 2011 by local and national press of the mega event. A content analysis of the news published covering the period between January and December 2011 and using three newspapers was conducted. From those, two were local and weekly newspapers and one was a national daily one. Looking to data results, it can be concluded that it was poor the community involvement and, also, the one of the cultural associations in the organizations of the 2012 ECC. A strong negative reaction to the model choose to plan the mega event conducted by official organizers was found, which has cast doubts on the desirable participation of the residents and, consequently, on the success of the mega event, especially in a perspective of a medium and long term effects.

Multiple centroid method to evaluate the adaptability of alfalfa genotypes

This study aimed to evaluate the efficiency of multiple centroids to study the adaptability of alfalfa genotypes (Medicago sativa L.). In this method, the genotypes are compared with ideotypes defined by the bissegmented regression model, according to the researcher's interest. Thus, genotype classification is carried out as determined by the objective of the researcher and the proposed recommendation strategy. Despite the great potential of the method, it needs to be evaluated under the biological context (with real data). In this context, we used data on the evaluation of dry matter production of 92 alfalfa cultivars, with 20 cuttings, from an experiment in randomized blocks with two repetitions carried out from November 2004 to June 2006. The multiple centroid method proved efficient for classifying alfalfa genotypes. Moreover, it showed no unambiguous indications and provided that ideotypes were defined according to the researcher's interest, facilitating data interpretation.

Access to information: assessment of the use of automated interaction technologies in call centers

With the purpose of at lowering costs and reendering the demanded information available to users with no access to the internet, service companies have adopted automated interaction technologies in their call centers, which may or may not meet the expectations of users. Based on different areas of knowledge (man-machine interaction, consumer behavior and use of IT) 13 propositions are raised and a research is carried out in three parts: focus group, field study with users and interviews with experts. Eleven automated service characteristics which support the explanation for user satisfaction are listed, a preferences model is proposed and evidence in favor or against each of the 13 propositions is brought in. With balance scorecard concepts, a managerial assessment model is proposed for the use of automated call center technology. In future works, the propositions may become verifiable hypotheses through conclusive empirical research.