Comparative effect of FGF2, synthetic peptides 1-28 N-POMC and ACTH on proliferation in rat adrenal cell primary cultures

There is evidence that pro-opiomelanocortin (POMC)-derived peptides other than adrenocorticotropic hormone (ACTH) have a role in adrenal cell proliferation. We compared the activity of synthetic rat N-terminal POMC fragment 1-28 with disulfide bridges (N-POMC(w)) and without disulfide bridges (N-POMC(w/o)), with the activity of fibroblast growth factor (FGF2), a widely studied adrenal growth factor, and ACTH, in well-characterized pure cultures of both isolated adrenal Glomerulosa (G) and Fasciculata/Reticularis (F/R) cells. Three days of FGF2-treatment had a proliferative effect similar to serum, and synthetic peptide N-POMC(w) induced proliferation more efficiently than N-POMC(w/o). Moreover, both induced proliferation via the ERK1/2 pathway. In contrast, sustained ACTH treatment decreased proliferation and viability through apoptosis induction, but not necrosis, and independently of PKA and PKC pathways. Further elucidation of 1-28 POMC signal transduction is of interest, and primary cultures of adrenal cells were found to be useful for examining the trophic activity of this peptide.

Immobilization of primary cultures of insulin-releasing human pancreatic cells

Transplantation of pancreatic islets isolated from organ donors constitutes a promising alternative treatment for type 1 diabetes, however, it is severely limited by the shortage of organ donors. Ex vivo islet cell cultures appear as an attractive but still elusive approach for curing type 1 diabetes. It has recently been shown that, even in the absence of fibrotic over-growth, several factors, such as insufficient nutrition of the islet core, represent a major barrier for long-term survival of islets grafts. The use of immobilized dispersed cells may contribute to solve this problem due to conceivably easier nutritional and oxygen support to the cells. Therefore, we set out to establish an immobilization method for primary cultures of human pancreatic cells by adsorption onto microcarriers (MCs). Dispersed human islets cells were seeded onto Cytodex1 microcarriers and cultured in bioreactors for up to eight days. The cell number increased and islet cells maintained their insulin secretion levels throughout the time period studied. Moreover, the cells also presented a tendency to cluster upon five days culturing. Therefore, this procedure represents a useful tool for controlled studies on islet cells physiology and, also, for biotechnological applications.

Scanlation - What Fan Translators of Manga Learn in the Informal Learning Environment

The present paper discusses two pilot studies carried out to see the possibility of the fan community of manga (Japanese comics), in which fan translators translate the original Japanese manga into English (which is called scanlation), functioning as an informal learning environment for the Japanese language learning and translator training. Two pilot studies consist of a) comparison of the original Japanese version with the scanlation and official translation, and b) comparison of the original Japanese version with two different versions of scanlation to see the translators’ level of Japanese language and the overall translation quality. The results show that in scanlation versions, there were numbers of inaccuracies which would prevent them to be treated as professional translation. Some of these errors are clearly caused by insufficient understanding of Japanese language by the translator. However, the pilot studies also suggested some interesting features of fan translation, such as the treatment of cultural references. The two pilot studies indicate that it is desirable to conduct further studies with more data, in order to confirm the results of present studies, and to see the possible relationship between the types of trnalsation errors found in scanlation and the particular type of Japanese language (informal, conversational) that could be learned from manga.

Fan fictions eller adaptioner? : Om Amy Heckerlings spelfilm Clueless (1996) och Debra White Smiths roman Amanda (2006) mot Jane Austens roman Emma (1815)

Det huvudsakliga syftet med min uppsats var att utifrån originalverket Emma (1815) avJane Austen kunna bedöma vad två verk som inspirerats av romanen bör klassificeras som.De två verken var romanen Amanda (2006) av författaren Debra White Smith ochspelfilmen Clueless (1995) av regissören Amy Heckerling. Till min hjälp har jag lästaktuell forskning inom de två termerna adaption och fanfiction och utifrån detta gjort enjämförande analys på verken. Resultatet blev oväntat vagt, det visade sig att begreppen lågnärmre varandra än vad jag trott från början. Båda verken kan till viss del ses som enadaption på originalverket, på samma sätt som de även kan ses som fanfiction. Slutsatsenär dock att de båda verken passar bäst in under termen profic, som är en underkategoriinom fanfiction där författaren/regissören tjänar pengar på sin modifikation och intepublicerat verket i exempelvis ett obetalt nätforum som hobbyaktivitet.

Fan Culture as an Informal Learning Environment : Presentation of a NGL project

Fan culture is a subculture that has developed explosively on the internet over the last decades. Fans are creating their own films, translations, fiction, fan art, blogs, role play and also various forms that are all based on familiar popular culture creations like TV-series, bestsellers, anime, manga stories and games. In our project, we analyze two of these subculture genres, fan fiction and scanlation. Amateurs, and sometimes professional writers, create new stories by adapting and developing existing storylines and characters from the original. In this way, a "network" of texts occurs, and writers step into an intertextual dialogue with established writers such as JK Rowling (Harry Potter) and Stephanie Meyer (Twilight). Literary reception and creation then merge into a rich reciprocal creative activity which includes comments and feedback from the participators in the community. The critical attitude of the fans regarding quality and the frustration at waiting for the official translation of manga books led to the development of scanlation, which is an amateur translation of manga distributed on the internet.  Today, young internet users get involved in conceptual discussions of intertextuality and narrative structures through fan activity. In the case of scanlation, the scanlators practice the skills and techniques of translating in an informal environment. This phenomenon of participatory culture has been observed by scholars and it is concluded that they contribute to the development of a student’s literacy and foreign language skills. Furthermore, there is no doubt that the fandom related to Japanese cultural products such as manga, anime and videogames is one of the strong motives for foreign students to start learning Japanese. This is something to take into pedagogical consideration when we develop web-based courses. Fan fiction and fan culture make it ​​possible to have an intensive transcultural dialogue between participators throughout the world and is of great interest when studying the interaction between formal and informal learning that puts the student in focus

"Men shit, hur fan har vi kunnat glömma bort det?" : Falu kommuns socialtjänsts arbete gällande prostitution

The purpose of this study was to examine whether and how the social services in the municipality of Falun is managing social work related to prostitution. It is a qualitative study based on three focus group interviews conducted in parts of the social services organization in the municipality of Falun. The empirical data collected was analyzed from an intersectional perspective. Several distinct findings emerged from the study. Social work against prostitution does not exist in the social services organization in the municipality of Falun. The organization possesses no procedures or guidelines for this kind of work, and no preventive work or cooperation with other organizations is carried out. It also emerged, that several social work officers had a stereotype image of who a potential sex- seller could be. This fact may influence who would be able to get any support from social services regarding to this social problem.

"Jag slåss med rosa handskar, men de är fan inte fluffiga" : En kvalitativ studie om verksamheten Pink Gloves Boxing

Syftet med denna studie var att undersöka vilken betydelse verksamheten Pink Gloves Boxing haft för enskilda deltagares psykosociala livssituation, som öppenvårdsinsats i Borlänge kommun. Dessutom har syftet varit att undersöka vilka komponenter i verksamheten som bidragit till en eventuell förändring avseende psykosocial livssituation. Då studien ämnat fånga deltagares subjektiva upplevelser av Pink Gloves Boxing har det empiriska materialet insamlats genom semistrukturerade intervjuer med sex deltagare. Genom en tematisk analys av det insamlade materialet framkom centrala teman och subteman, vilka utgjort grunden i studiens resultatdel. För att analysera resultatet har en teoretisk tolkningsram tillämpats, vilken innefattat teorier om empowerment och genus. Samtliga informanter pekar på bättre fysik och ökad psykisk hälsa, av sitt deltagande i verksamheten. Stresshantering, aggressionskontroll och grupptillhörighet är exempel på förtjänster av ett deltagande, som informanterna framhållit. Gynnande komponenter som framträder i verksamheten är tränarnas roll, gruppen, träningsformen och verksamhetens upplägg, som exempelvis endast kvinnliga deltagare och gruppsamtal.

Engajamento com fan pages de marcas no Facebook: impacto da força do laço, homofilia e confiança

A literatura de relacionamento do consumidor com a marca tem reconhecido a importância do ambiente virtual como ferramenta eficaz para a promoção da marca. Este espaço permitiu uma maior proximidade e estreitamento das relações com os diversos públicos das empresas ao potencializar as funções do marketing, o que levou as empresas a criarem fan pages de marca a fim de promover maior interação e incrementar o relacionamento com os stakeholders. Esta dissertação tem como objetivo verificar o impacto da força do laço e da homofilia na confiança em uma fan page de marca, e o impacto da confiança no engajamento com esta fan page. Para atender a este objetivo, a pesquisa se deu em duas etapas: uma primeira fase qualitativa e uma segunda fase quantitativa. Na primeira fase, esta pesquisa identifica a unidade de análise dos conceitos e gera itens para a mensuração da força do laço, homofilia, confiança e engajamento, por meio da revisão de literatura e nove entrevistas com seguidores de fan pages de marcas. A segunda fase verifica, por meio de uma survey com 688 seguidores de uma fan page, o impacto destes conceitos no engajamento. As entrevistas foram analisadas por meio da análise de conteúdo, enquanto que os dados da survey e a rede nomológica foram analisados pela modelagem de equações estruturais. Os resultados da primeira fase indicam que os consumidores tratam a fan page como unidade de análise dos conceitos e forneceram itens satisfatórios para a mensuração dos conceitos e suas dimensões. Já os resultados da segunda fase dão suporte a rede nomológica testada, indicando uma associação positiva da força do laço e homofilia com a confiança, e da confiança com o engajamento com fan pages de marcas.

Cultures at work: the case of italian expatriates in Brazil

Esta tese de mestrado tem por intenção entender quais são as mais importantes diferenças culturais para os expatriados Italianos que trabalham no Brasil e aprender as implicações práticas para o ambiente de trabalho. O método utilizado foi qualitativo, com 23 entrevistas em profundidade com expatriados italianos de nível médio ate top management, com experiência de trabalho no Brasil. Os resultados indicam que os expatriados Italianos experimentam dificuldades com as diferenças em termos de comunicação, distinção entre as esfera profissional e privada, distancia do poder e planejamento. Em contrapartida, outros fatores como a discriminação positiva para os estrangeiros, diferenças em geneder equality e masculinidade, assim como com a atitude positiva dos workplaces e uma economia em crescimento, todos influenciam de maneira positiva a experiência do expatriado. Enfim, algumas sugestões práticas sobre os efeitos das diferenças culturais e sobre a estruturação de um possível cross-cultural training são expostas.

Leader-organization fit: comparing the effectiveness of paternalistic and transformational leadership in different organizational cultures

Research on paternalistic leadership (PL) has been based exclusively on national cultures´ differences. However there are cues that other contextual variables can add to the explanation of this construct. Due to its capacity to influence expectations of individuals in organizations, organizational culture can contribute to fill this gap. To test if organizational culture influences the effectiveness of leadership style, we conducted two experimental studies using Amazon’s Mechanical Turk, comparing effects of paternalistic and transformational leadership on followers’ outcomes. Using video clips and vignettes, we found that PL is better related to followers´ outcomes in cultures oriented to people than outcome, and that TL has a better relationship in cultures oriented to innovation than stability. The results suggest that organizational culture helps in explaining PL endorsement, and that further analysis of the influence of this variable to PL can provide a better understanding of the expression of this leadership style in organizations.

Cultural strategies: how do national cultures impact the operations strategy?

Through the assessment of the fourth round of the High Performance Manufacturing (HPM) project and the introduction of Hofstede’s Cultural Classification, the present work aims to deepen the comprehension of the impact of National Cultures on firms’ Operations Strategy. The ANOVA comparisons of four Operations Strategy elements in countries with different industrialization and development backgrounds (e.g. Germany, China, Brazil and South Korea) suggest that while Integrating Leadership and Implementation of Manufacturing Strategy are affected by the cultural levels of Power Distance, Individualism vs. Collectivism and Uncertainty Avoidance, the other two elements of Operations Strategy, Functional Integration and Formal Manufacturing Strategy, show effects of the degree of Individualism vs. Collectivism and Long-Term Orientation. The results of the study are expected to offer new perspectives on the planning and implementation of strategic and operations management for both practitioners and academics. More specifically, the analysis of cross-cultural influence over operations strategy may contribute to a better understanding of how cooperative behavior may lead firms to generate higher rents through the strengths and weaknesses of their relations, particularly in terms of global supply chains.

Large scale laboratory cultures of Ankistrodesmus gracilis (Reisch) Korsikov (Chlorophyta) and Diaphanosoma biergei Korinek, 1981 (Cladocera)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Anticandidal Efficacy of Cinnamon Oil Against Planktonic and Biofilm Cultures of Candida parapsilosis and Candida orthopsilosis

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)