Isolation and characterization of a novel variant of HMW glutenin subunit gene from the St genome of Pseudoroegneria stipifolia

The x- and y-type high molecular weight (HMW) glutenin subunits are conserved seed storage proteins in wheat and related species. Here we describe investigations on the HMW glutenin subunits from several Pseudoroegneria accessions. The electrophoretic mobilities of the HMW glutenin subunits from Pd. stipifolia, Pd tauri and Pd strigosa were much faster than those of orthologous wheat subunits, indicating that their protein size may be smaller than that of wheat subunits. The coding sequence of the Glu-1St1 subunit (encoded by the Pseudoroegneria stipifolia accession PI325181) was isolated, and found to represent the native open reading frame (ORF) by in vitro expression. The deduced amino acid sequence of Glu-1St1 matched with that determined from the native subunit by mass spectrometric analysis. The domain organization in Glu-1St1 showed high similarity with that of typical HMW glutenin subunits. However, Glu-1St1 exhibited several distinct characteristics. First, the length of its repetitive domain was substantially smaller than that of conventional subunits, which explains its much faster electrophoretic mobility in SDS-PAGE. Second, although the N-terminal domain of Glu-1St1 resembled that of y-type subunit, its C-terminal domain was more similar to that of x-type subunit. Third, the N- and C-terminat domains of Glu-1St1 shared conserved features with those of barley D-hordein, but the repeat motifs and the organization of its repetitive domain were more similar to those of HMW glutenin subunits than to D-hordein. We conclude that Glu-1St1 is a novel variant of HMW glutenin subunits. The analysis of Glu-1St1 may provide new insight into the evolution of HMW glutenin subunits in Triticeae species. (C) 2007 Elsevier Ltd. All rights reserved.

AVALIAÇÃO de procedências de erva-mate (Ilex paraguariensis St. Hil) para plantios solteiros e em sistemas agroflorestais.

2002

INFLUÊNCIA de coberturas verdes de inverno e de sistemas de preparo do solo na produtividade da erva-mate (Ilex paraguariensis St Hil.), em Ponta Grossa, PR.

2002

Thomas Griffin of Corca Dhuibhne (1829-96) and the Irish Community of Lawrence, Massachusetts

RAE2008

The Jesus Chapel in St Paul's Cathedral, London: a reconstruction of its appearance before the Reformation

New, Elizabeth, 'The Jesus Chapel in St Paul's Cathedral, London: a reconstruction of its appearance before the Reformation', Antiquaries Journal (2005) 85, pp.103-124 RAE2008

Lordship and the peasant economy, c.1250-c.1400: Robert Kyng and the Abbot of Bury St Edmunds

Schofield, P. (2007). Lordship and the peasant economy, c.1250-c.1400: Robert Kyng and the Abbot of Bury St Edmunds. Past and Present. 195(Sup. 2), pp.53-68. RAE2008

A Sermon preached in the Parish Church of St. Nicholas, in Bristol

Sermon.

A Chosen Witness: A Sermon Preached in St. Stephen's Church in Memory of the Rev. Webster

Memorial Sermon preached in memory of the Rev. Walter Gardner Webster

Life and letters of Fathers Ponziglione, Schoenmakers, and other early Jesuits at Osage mission. Sketch of St. Francis' church. Life of Mother Bridget. By W.W. Graves . .

http://www.archive.org/details/ponziglionescho00gravrich

Historical sketch of the Flathead Indian nation from the year 1813 to 1890 : embracing the history of the establishment of St. Mary's Indian Mission in the Bitter Root Valley, Mont. : with sketches of the missionary life of Father Ravalli and other early missionaries : wars of the Blackfeet and Flatheads and sketches of history, trapping and trading in the early days / by Peter Ronan.

http://www.canadiana.org/ECO/mtq?doc=16974 View document online

Relation de la Mission Abnaquise de St. Franðcois de Sales L'Annâee 1702 / par le Páere Jacques Bigot, de la Compagnie de Jâesus.

http://www.archive.org/details/missionabnaquise00bigorich

Father Pollock and his brother : mission priests of St. Alban's, Birmingham / with a letter from Charles Gore.

http://anglicanhistory.org/bios/pollock/ View document online