Immunomarquage des collagènes I, III et IV, de la laminine et de la fibronectine dans la capsule cristallinienne humaine [Immunolabelling of collagen types I, III and IV, laminin and fibronectin in the human lens capsule].

PURPOSE: To localize collagen types I, III, and IV, laminin and fibronectin in the anterior human lens capsule. MATERIAL AND METHODS: Twenty-one anterior capsules were sampled by capsulorhexis during extracapsular cataract extraction (mean age 71.5). All capsules were labelled by an immunostaining specific for each antibodies. Immunostaining of four capsules was revealed with immunoperoxydase and seventeen using indirect immunofluorescence. RESULTS: Labelling of collagen types I and III was observed throughout the entire thickness of the capsule for each technique, the strongest labelling was found in the base of the epithelial cells with immunofluorescence. Collagen type IV was observed at the base of the epithelial cells whichever technique was used. Laminin could be detected in the inner layer of the capsule, using immunoperoxydase or immunofluorescence. No specific labelling was found for fibronectin using the two techniques. CONCLUSIONS: Different kinds of collagens have been found in capsules, more particularly the type III. The latter does not appear on other ocular basement membrane. Because of this uneven distribution in the capsule's thickness, each collagen might have a specific function.

Las “nuevas guerras” del siglo XXI. Tendencias de la conflictividad armada contemporánea

El paper analiza los cambios y las continuidades en los conflictos armados de alta intensidad, las guerras, en el siglo XXI, también llamadas “nuevas guerras”. Se propone un patrón de análisis en torno a diez variables: el número de conflictos, el nivel de intensidad, las víctimas que producen, el contexto espacial en que se desarrollan, las causas que los ocasionan y los objetivos que persiguen las partes, las tácticas e instrumentos de combate utilizados, la economía de los conflictos, las pautas regionales y la relación entre conflictividad armada de alta intensidad y seguridad. Se defiende que las trasformaciones experimentadas en la pauta de conflictividad armada no han conducido a un mundo más seguro sino que, al contrario, han exacerbado la dificultad de gestión de los conflictos debido a la mayor complejidad de los mismos, tanto por la multicausalidad de las incompatibilidades y el carácter estructural de muchas de ellas, como por el mayor número de actores y sobre todo por su diversidad, vinculado al hecho de que las normas e instituciones tradicionales de gestión de conflictos están pensadas para un tipo de conflictividad –la interestatal– en clara regresión, mientras que seguimos sin disponer de instrumentos normativos vinculantes adaptados a las nuevas necesidades, y los que existen no son aceptados por todos los Estados ni siquiera por todas las grandes potencias del sistema interestatal.

Collection complète, par ordre chronologique, des lois, édits, traités de paix, ordonnances, déclarations et règlemens d'intérêt général antérieurs à 1789 restés en vigueur : avec des renvois à la législation nouvelle et à la jurisprudence... et une table raisonnée des matières.... IV. [1768-1778] / par M. Walker,...

[Acte royal. 0420-1789]

CRESCIMENTO E OXIDAÇÃO DE EXPLANTES DE BANANEIRA PRATA (Musa AAB) IN VITRO: IV. CONCENTRAÇÕES DE SAIS, ÁCIDOS ASCÓRBICOS E FREQÜÊNCIA DE SUBCULTIVOS

Avaliaram-se diferentes concentrações de sais do meio MS (Murashige & Skoog, 1962), freqüência de subcultivos e adição de ácido ascórbico ao meio de cultura, objetivando o controle da oxidação de explantes de bananeira-'Prata' (Musa AAB) na fase de estabelecimento. Os tratamentos constituíram-se das diluições dos sais do meio MS (100%, 50% e 33,33%), subcultivos (a cada 7 dias, a cada 14 dias e a cada 28 dias) e ácido ascórbico (0 e 25 mg L-1). O delineamento experimental foi inteiramente casualizado, cujos tratamentos foram arranjados em um fatorial 3 x 2 x 2. Utilizaram-se 15 repetições. As avaliações relativas à massa da matéria fresca, altura e oxidação foram feitas aos 28 dias após a inoculação. Quando o período de subcultivos foi maior (28 dias), o crescimento em massa da matéria fresca foi reduzido em função da redução da concentração de sais do meio MS. Para meios de cultura menos concentrados e freqüência maiores de subcultivos, não houve necessidade da adição do ácido ascórbico para a redução do escurecimento e houve uma tendência de maior crescimento em massa dos explantes.

20/4/20, M. Deschanel arrive à la caserne Henri IV pour décorer les gendarmes des Aubrais : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58965

20/4/20, M. Deschanel à la caserne Henri IV [défilé de gendarmes] : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 58970

Dictionnaire historique et critique. T. IV, Bosc-Caussin / de Pierre Bayle... ; éd. augm. de notes extraites de Chaufepié, Joly, La Monnoie, Leduchat, L.-J. Leclerc, Prosper Marchand, etc...

Collection : Archives de la linguistique française ; 30

Thrombocytosis as a prognostic marker in stage III and IV serous ovarian cancer.

OBJECTIVE: Thrombocytosis is an adverse prognostic factor in many types of cancer. We investigated if pre-treatment increased platelet counts provide prognostic information specifically in patients with stage III and IV serous ovarian cancer which is the most common clinical presentation of ovarian cancer. METHODS: Platelet number on diagnosis of stage III and IV serous ovarian adenocarcinoma was evaluated in 91 patients for whom there were complete follow-up data on progression and survival. Survival and progression free survival of patients with normal platelet counts (150-350 ×10(9)/L) was compared with that of patients with thrombocytosis (>350×10(9)/L) by χ(2) and logrank tests. RESULTS: The median age of the patients was 66 years-old. From the 91 patients, 52 (57.1%) had normal platelet counts (median, 273×10(9)/L; range, 153-350) at diagnosis of their disease and 39 patients (42.9%) had thrombocytosis (median, 463×10(9)/L; range, 354-631). In the group of patients with normal platelet counts, 24 of the 52 patients had died with a median survival of 43 months (range, 3-100). In the group of patients with thrombocytosis, 24 of the 39 patients had died with a median survival of 23 months (range, 4-79). In the entire group of 91 patients there was a statistically significant difference of the overall survival and progression-free survival between the two groups (logrank test P=0.02 and P=0.007, respectively). CONCLUSION: In this retrospective analysis of stage III and IV ovarian cancer patients, thrombocytosis at the time of diagnosis had prognostic value regarding overall survival and progression-free survival.