Secretaria on-line from ISCAP

Like many other higher educat ion schools, ISCAP`s population has grown at a rate of almost 100% in the end of the twentieth century. Its administrative structures were reinforced, but it was not in the same proportion. Face to face with the inability to resolve the problem, the administration decided to implement a computer based system, available in the Internet. In a first stage, in 1997, the system was implemented as a services support. The next stage, in 1999, proposes to increase student services quality. A project that aims to bring student services available on the Internet begins to be developed.

Aplicação das tecnologias de informação e comunicação como estratégia para a retenção de aunos

Este artigo aborda a aplicação das Tecnologias de Informação e Comunicação como estratégias de boas práticas que conduzem à retenção e captura de alunos do ensino superior. É baseado num estudo de caso de oito anos, de utilização de um completo sistema de informação. Este sistema, além de constituir um ERP, de suporte às actividades de gestão académica, dispõe ainda de uma forte componente de SRM que confere suporte às actividades administrativas e lectivas. É descrito em que medida o sistema apresentado facilita a interacção e comunicação entre os membros da comunidade académica, recorrendo à internet, com serviços disponíveis na Web complementando-os com correio electrónico, SMS e CTI. Através de uma percepção, sustentada por análise empírica e por resultados de inquéritos, demonstra-se como este tipo de boas práticas pode elevar o nível de satisfação da comunidade. Muito em particular, é possível combater o insucesso escolar, evitar que alunos abandonem os seus cursos antes do seu término e que os recomendem a potenciais alunos. Em complemento, este tipo de estratégia permite ainda fortes economias na gestão da instituição, elevando o seu valor. Como trabalho futuro, é apresentada a nova fase do projecto que envereda pela aplicação de Business Intelligence para optimização do processo de gestão, tornando-o pró-activo. Também é apresentada a visão tecnológica que orienta os novos desenvolvimentos, para uma arquitectura baseada em serviços Web e linguagens de definição processual.

Technology strategies in the process of student retention and efficiency of academic management

This article discusses the application of Information and Communication Technologies and strategies for best practices in order to capture and maintain faculty students' attention. It is based on a case study of ten years, using a complete information system. This system, in addition to be considered an ERP, to support the activities of academic management, also has a strong component of SRM that provides support to academic and administrative activities. It describes the extent to which the presented system facilitates the interaction and communication between members of the academic community, using the Internet, with services available on the Web complementing them with email, SMS and CTI. Through a perception, backed by empirical analysis and results of investigations, it demonstrates how this type of practice may raise the level of satisfaction of the community. In particular, it is possible to combat failure at school, avoid that students leave their course before its completion and also that they recommend them to potential students. In addition, such a strategy also allows strong economies in the management of the institution, increasing its value. As future work, we present the new phase of the project towards implementation of Business Intelligence to optimize the management process, making it proactive. The technological vision that guides new developments to a construction based on Web services and procedural languages is also presented.

Ne bis in idem -periaate ympäristörikosoikeudessa – Hallinnollisten seuraamusten ja rikosoikeudellisen rangaistuksen suhde

Tutkielma käsittelee ne bis in idem -periaatteen toteutumista kotimaisen ympäristörikosoikeuden alalla, etenkin hallinnollisten seuraamusten ja rikosoikeudellisten rangaistusten välisissä suhteissa. Erityisesti Euroopan ihmisoikeussopimuksen kautta Suomenkin lainkäyttöön ja -säädäntöön vaikuttava periaate estää syyttämisen ja rankaisemisen kahdesti samasta teosta. Periaate on kehittynyt Euroopan ihmisoikeustuomioistuimen oikeuskäytännön myötä viime vuosina, minkä vuoksi Suomessakin on jouduttu muuttamaan käsityksiä sanktioiden rangaistusluonteesta, saman asian käsitteestä ja päätöksen lopullisuudesta. Myös tuomioistuimet ovat joutuneet ottamaan kantaa ympäristörikosoikeuden ne bis in idem -väitteisiin. Tutkielmassa arvioidaan ne bis in idem -periaatteen kehittymistä ja uutta tulkintaa sekä niiden vaikutusta erityisesti ympäristöoikeuden hallintosanktioista öljypäästömaksun ja jätelain laiminlyöntimaksun sekä hallintopakon alalta uhkasakon rangaistusluonteisiin. Tutkimuskysymystä lähestytään lainopillisesti sekä aihetta käsittelevän kirjallisuuden että relevanttien oikeustapausten kautta. Keskeisimmäksi lopputulemaksi muodostuu, että ympäristöoikeuden hallintosanktiot ja hallintopakko saattavat tietyin edellytyksin yhdessä rikosoikeudellisen rangaistuksen kanssa aiheuttaa ne bis in idem -tilanteita Euroopan ihmisoikeustuomioistuimen uuden tulkintakäytännön myötä. Periaatteen ollessa yhä kehittymässä niin ympäristöllisten kuin muidenkin hallintosanktioiden ja hallintopakon osalta periaatteen toteutuminen näyttäytyy yhä epäselvänä. Tutkimuskysymys sijoittuu ympäristöoikeuden, rikosoikeuden, prosessioikeuden ja hallinto-oikeuden rajapinnalle, joten tutkielma on oikeudenalat ylittävä. Ympäristöoikeuden esimerkkien kautta näkökulmaa laajennetaan myös muiden hallintosanktioiden kokonaisuuteen sekä tarkoitukseen ne bis in idem -näkökulmasta. Tutkielman lopussa esitetyissä de lege ferenda -näkemyksissä esitetään erityisesti, että hallinnollisia sanktioita tulisi tarkastella lainsäädännössä kokonaisuutena sekä oikeusturvan että tehokkaan ympäristönsuojelun takaamiseksi, ja että rikosoikeuden havaittuihin ongelmiin tulisi ensisijaisesti puuttua hallinnollisten keinojen sijaan rikosoikeudellisilla keinoilla.

Desenvolvimento de método empregando DLLME para extração e pré-concentração de ferro em amostras de água com diferentes salinidades

Neste trabalho foi desenvolvido e validado um método analítico para a determinação de Fe total em amostras de água com diferentes salinidades empregando a microextração líquido-líquido dispersiva (DLLME), seguido de determinação espectrofotométrica na região do ultravioleta-visível. Nas extrações por DLLME, o Fe foi complexado com pirrolidina ditiocarbamato de amônio (APDC) e extraído após a injeção de uma mistura composta de 1,2-diclorobenzeno (solvente extrator) e etanol (solvente dispersor). Os fatores que influenciam a eficiência de extração (tipo e volume de solvente extrator e dispersor, concentração de APDC e tempo de extração) e subsequente determinação espectrofotométrica foram otimizados. Após otimização das condições, a curva de calibração foi linear entre 0,02 e 2,0 mg L-1 (r 2 = 0,9998) e os limites de detecção e quantificação do método foram de 6,1 e 18,3 µg L-1 , respectivamente. O fator de pré-concentração foi de 3,3. A exatidão foi avaliada em termos de recuperação, com variação entre 90 e 102% e desvio padrão relativo (RSD) inferiores a 12%. A exatidão do método também foi avaliada utilizando um material de referência certificado (SRM 1643e, Elementos Traço em Água), o qual apresentou concordância de 104% e RSD de 5%. O método foi aplicado para amostras de água com diferentes salinidades, como água mineral, estuarina e de alto mar. Além disso, o método proposto apresentou concordância da ordem de 95%, quando comparado com o método 1,10-fenantrolina para a determinação de Fe total.

Ne bis in idem -periaate ympäristörikosoikeudessa – Hallinnollisten seuraamusten ja rikosoikeudellisen rangaistuksen suhde

Tutkielma käsittelee ne bis in idem -periaatteen toteutumista kotimaisen ympäristörikosoikeuden alalla, etenkin hallinnollisten seuraamusten ja rikosoikeudellisten rangaistusten välisissä suhteissa. Erityisesti Euroopan ihmisoikeussopimuksen kautta Suomenkin lainkäyttöön ja -säädäntöön vaikuttava periaate estää syyttämisen ja rankaisemisen kahdesti samasta teosta. Periaate on kehittynyt Euroopan ihmisoikeustuomioistuimen oikeuskäytännön myötä viime vuosina, minkä vuoksi Suomessakin on jouduttu muuttamaan käsityksiä sanktioiden rangaistusluonteesta, saman asian käsitteestä ja päätöksen lopullisuudesta. Myös tuomioistuimet ovat joutuneet ottamaan kantaa ympäristörikosoikeuden ne bis in idem -väitteisiin. Tutkielmassa arvioidaan ne bis in idem -periaatteen kehittymistä ja uutta tulkintaa sekä niiden vaikutusta erityisesti ympäristöoikeuden hallintosanktioista öljypäästömaksun ja jätelain laiminlyöntimaksun sekä hallintopakon alalta uhkasakon rangaistusluonteisiin. Tutkimuskysymystä lähestytään lainopillisesti sekä aihetta käsittelevän kirjallisuuden että relevanttien oikeustapausten kautta. Keskeisimmäksi lopputulemaksi muodostuu, että ympäristöoikeuden hallintosanktiot ja hallintopakko saattavat tietyin edellytyksin yhdessä rikosoikeudellisen rangaistuksen kanssa aiheuttaa ne bis in idem -tilanteita Euroopan ihmisoikeustuomioistuimen uuden tulkintakäytännön myötä. Periaatteen ollessa yhä kehittymässä niin ympäristöllisten kuin muidenkin hallintosanktioiden ja hallintopakon osalta periaatteen toteutuminen näyttäytyy yhä epäselvänä. Tutkimuskysymys sijoittuu ympäristöoikeuden, rikosoikeuden, prosessioikeuden ja hallinto-oikeuden rajapinnalle, joten tutkielma on oikeudenalat ylittävä. Ympäristöoikeuden esimerkkien kautta näkökulmaa laajennetaan myös muiden hallintosanktioiden kokonaisuuteen sekä tarkoitukseen ne bis in idem -näkökulmasta. Tutkielman lopussa esitetyissä de lege ferenda -näkemyksissä esitetään erityisesti, että hallinnollisia sanktioita tulisi tarkastella lainsäädännössä kokonaisuutena sekä oikeusturvan että tehokkaan ympäristönsuojelun takaamiseksi, ja että rikosoikeuden havaittuihin ongelmiin tulisi ensisijaisesti puuttua hallinnollisten keinojen sijaan rikosoikeudellisilla keinoilla.

EFFECT OF SORGHUM SEED TREATMENT IN BURKINA FASO VARIES WITH BASELINE CROP PERFORMANCE AND GEOGRAPHICAL LOCATION

Sorghum [ Sorghum bicolor (L.) Moench] is a major subsistence crop throughout the region of Sahel. With the exception of seeds and labour, no agricultural inputs are in general used in sorghum production since the grain is of a relatively low commercial value and the risk of losing the crop to drought, flooding, etc. is substantial. A meta-analysis of 118 field experiments was carried out to identify conditions in which two protective seed treatments could support a yield increase of sorghum in Burkina Faso. The two treatments were: i) treatment with the pesticide Calthio C (thiram and chlorpyrifos) and ii) treatment with an aqueous extract from the plant Eclipta alba . Both treatments were found to produce a yield increase (Medians: Calthio C +199 kg ha-1, P<2x10-9; E. alba +90.5 kg ha-1 P<4x10-4). A strong relative effect of Calthio C on yield (+36%) was found for field experiments with a low baseline yield. A strong relative effect of E. alba extract on yield (+22%) was found for experiments with a low baseline of emergence. ANOVA of the 118 field tests showed that baseline crop performance (yield and emergence) and the effect of seed treatments were strongly linked to geographical location (twelve different villages included). Roots from sorghum in the village showing the strongest effect of both seed treatments (>40% yield increase) were found to carry a comparatively high load of the infectious ascomycetes: Fusarium equiseti , Macrophomina phaseolina and Curvularia lunata .

Modifications neurométaboliques et microstructurales à la suite d'une commotion cérébrale chez les athlètes féminines

L’utilisation de méthodes d’investigation cérébrale avancées a permis de mettre en évidence la présence d’altérations à court et à long terme à la suite d’une commotion cérébrale. Plus spécifiquement, des altérations affectant l’intégrité de la matière blanche et le métabolisme cellulaire ont récemment été révélées par l’utilisation de l’imagerie du tenseur de diffusion (DTI) et la spectroscopie par résonance magnétique (SRM), respectivement. Ces atteintes cérébrales ont été observées chez des athlètes masculins quelques jours après la blessure à la tête et demeuraient détectables lorsque les athlètes étaient à nouveau évalués six mois post-commotion. En revanche, aucune étude n’a évalué les effets neurométaboliques et microstructuraux dans la phase aigüe et chronique d’une commotion cérébrale chez les athlètes féminines, malgré le fait qu’elles présentent une susceptibilité accrue de subir ce type de blessure, ainsi qu’un nombre plus élevé de symptômes post-commotionnels et un temps de réhabilitation plus long. Ainsi, les études composant le présent ouvrage visent globalement à établir le profil d’atteintes microstructurales et neurométaboliques chez des athlètes féminines par l’utilisation du DTI et de la SRM. La première étude visait à évaluer les changements neurométaboliques au sein du corps calleux chez des joueurs et joueuses de hockey au cours d’une saison universitaire. Les athlètes ayant subi une commotion cérébrale pendant la saison ont été évalués 72 heures, 2 semaines et 2 mois après la blessure à la tête en plus des évaluations pré et post-saison. Les résultats démontrent une absence de différences entre les athlètes ayant subi une commotion cérébrale et les athlètes qui n’en ont pas subie. De plus, aucune différence entre les données pré et post-saison a été observée chez les athlètes masculins alors qu’une diminution du taux de N-acetyl aspartate (NAA) n’a été mise en évidence chez les athlètes féminines, suggérant ainsi un impact des coups d’intensité sous-clinique à la tête. La deuxième étude, qui utilisait le DTI et la SRM, a révélé des atteintes chez des athlètes féminines commotionnées asymptomatiques en moyenne 18 mois post-commotion. Plus spécifiquement, la SRM a révélé une diminution du taux de myo-inositol (mI) au sein de l’hippocampe et du cortex moteur primaire (M1) alors que le DTI a mis en évidence une augmentation de la diffusivité moyenne (DM) dans plusieurs faisceaux de matière blanche. De iii plus, une approche par région d’intérêt a mis en évidence une diminution de la fraction d’anisotropie (FA) dans la partie du corps calleux projetant vers l’aire motrice primaire. Le troisième article évaluait des athlètes ayant subi une commotion cérébrale dans les jours suivant la blessure à la tête (7-10 jours) ainsi que six mois post-commotion avec la SRM. Dans la phase aigüe, des altérations neuropsychologiques combinées à un nombre significativement plus élevé de symptômes post-commotionnels et dépressifs ont été trouvés chez les athlètes féminines commotionnées, qui se résorbaient en phase chronique. En revanche, aucune différence sur le plan neurométabolique n’a été mise en évidence entre les deux groupes dans la phase aigüe. Dans la phase chronique, les athlètes commotionnées démontraient des altérations neurométaboliques au sein du cortex préfrontal dorsolatéral (CPDL) et M1, marquées par une augmentation du taux de glutamate/glutamine (Glx). De plus, une diminution du taux de NAA entre les deux temps de mesure était présente chez les athlètes contrôles. Finalement, le quatrième article documentait les atteintes microstructurales au sein de la voie corticospinale et du corps calleux six mois suivant une commotion cérébrale. Les analyses n’ont démontré aucune différence au sein de la voie corticospinale alors que des différences ont été relevées par segmentation du corps calleux selon les projections des fibres calleuses. En effet, les athlètes commotionnées présentaient une diminution de la DM et de la diffusivité radiale (DR) au sein de la région projetant vers le cortex préfrontal, un volume moindre des fibres de matière blanche dans la région projetant vers l’aire prémotrice et l’aire motrice supplémentaire, ainsi qu’une diminution de la diffusivité axiale (DA) dans la région projetant vers l’aire pariétale et temporale. En somme, les études incluses dans le présent ouvrage ont permis d’approfondir les connaissances sur les effets métaboliques et microstructuraux des commotions cérébrales et démontrent des effets délétères persistants chez des athlètes féminines. Ces données vont de pair avec la littérature scientifique qui suggère que les commotions cérébrales n’entraînent pas seulement des symptômes temporaires.

Reliability of graphite furnace atomic absorption spectrometry as alternative method for trace analysis of arsenic in natural medicinal products

Purpose: To evaluate the comparative efficiency of graphite furnace atomic absorption spectrometry (GFAAS) and hydride generation atomic absorption spectrometry (HGAAS) for trace analysis of arsenic (As) in natural herbal products (NHPs). Method: Arsenic analysis in natural herbal products and standard reference material was conducted using atomic absorption spectrometry (AAS), namely, hydride generation AAS (HGAAS) and graphite furnace (GFAAS). The samples were digested with HNO3–H2O2 in a ratio of 4:1 using microwaveassisted acid digestion. The methods were validated with the aid of the standard reference material 1515 Apple Leaves (SRM) from NIST Results: Mean recovery of three different samples of NHPs, using HGAAS and GFAAS, ranged from 89.3 - 91.4 %, and 91.7 - 93.0 %, respectively. The difference between the two methods was insignificant. A (P= 0.5), B (P=0.4) and C (P=0.88) Relative standard deviation (RSD) RSD, i.e., precision was 2.5 - 6.5 % and 2.3 - 6.7 % using HGAAS and GFAAS techniques, respectively. Recovery of arsenic in SRM was 98 and 102 % by GFAAS and HGAAS, respectively. Conclusion: GFAAS demonstrates acceptable levels of precision and accuracy. Both techniques possess comparable accuracy and repeatability. Thus, the two methods are recommended as an alternative approach for trace analysis of arsenic in natural herbal products.

Assessment of the Occurrence and Potential Risks of Antibiotics and their Metabolites in South Florida Waters Using Liquid Chromatography Tandem Mass Spectrometry

An automated on-line SPE-LC-MS/MS method was developed for the quantitation of multiple classes of antibiotics in environmental waters. High sensitivity in the low ng/L range was accomplished by using large volume injections with 10-mL of sample. Positive confirmation of analytes was achieved using two selected reaction monitoring (SRM) transitions per antibiotic and quantitation was performed using an internal standard approach. Samples were extracted using online solid phase extraction, then using column switching technique; extracted samples were immediately passed through liquid chromatography and analyzed by tandem mass spectrometry. The total run time per each sample was 20 min. The statistically calculated method detection limits for various environmental samples were between 1.2 and 63 ng/L. Furthermore, the method was validated in terms of precision, accuracy and linearity. The developed analytical methodology was used to measure the occurrence of antibiotics in reclaimed waters (n=56), surface waters (n=53), ground waters (n=8) and drinking waters (n=54) collected from different parts of South Florida. In reclaimed waters, the most frequently detected antibiotics were nalidixic acid, erythromycin, clarithromycin, azithromycin trimethoprim, sulfamethoxazole and ofloxacin (19.3-604.9 ng/L). Detection of antibiotics in reclaimed waters indicates that they can’t be completely removed by conventional wastewater treatment process. Furthermore, the average mass loads of antibiotics released into the local environment through reclaimed water were estimated as 0.248 Kg/day. Among the surface waters samples, Miami River (reaching up to 580 ng/L) and Black Creek canal (up to 124 ng/L) showed highest concentrations of antibiotics. No traces of antibiotics were found in ground waters. On the other hand, erythromycin (monitored as anhydro erythromycin) was detected in 82% of the drinking water samples (n.d-66 ng/L). The developed approach is suitable for both research and monitoring applications. Major metabolites of antibiotics in reclaimed wates were identified and quantified using high resolution benchtop Q-Exactive orbitrap mass spectrometer. A phase I metabolite of erythromycin was tentatively identified in full scan based on accurate mass measurement. Using extracted ion chromatogram (XIC), high resolution data-dependent MS/MS spectra and metabolic profiling software the metabolite was identified as desmethyl anhydro erythromycin with molecular formula C36H63NO12 and m/z 702.4423. The molar concentration of the metabolite to erythromycin was in the order of 13 %. To my knowledge, this is the first known report on this metabolite in reclaimed water. Another compound acetyl-sulfamethoxazole, a phase II metabolite of sulfamethoxazole was also identified in reclaimed water and mole fraction of the metabolite represent 36 %, of the cumulative sulfamethoxazole concentration. The results were illustrating the importance to include metabolites also in the routine analysis to obtain a mass balance for better understanding of the occurrence, fate and distribution of antibiotics in the environment. Finally, all the antibiotics detected in reclaimed and surface waters were investigated to assess the potential risk to the aquatic organisms. The surface water antibiotic concentrations that represented the real time exposure conditions revealed that the macrolide antibiotics, erythromycin, clarithromycin and tylosin along with quinolone antibiotic, ciprofloxacin were suspected to induce high toxicity to aquatic biota. Preliminary results showing that, among the antibiotic groups tested, macrolides posed the highest ecological threat, and therefore, they may need to be further evaluated with, long-term exposure studies considering bioaccumulation factors and more number of species selected. Overall, the occurrence of antibiotics in aquatic environment is posing an ecological health concern.

Homotheties and topology of tangent sphere bundles

We prove a Theorem on homotheties between two given tangent sphere bundles SrM of a Riemannian manifold (M,g) of dim ≥ 3, assuming different variable radius functions r and weighted Sasaki metrics induced by the conformal class of g. New examples are shown of manifolds with constant positive or with constant negative scalar curvature which are not Einstein. Recalling results on the associated almost complex structure I^G and symplectic structure ω^G on the manifold TM , generalizing the well-known structure of Sasaki by admitting weights and connections with torsion, we compute the Chern and the Stiefel-Whitney characteristic classes of the manifolds TM and SrM.

Desempenho de genótipos de girassol em Jaguariúna - SP - Análise preliminar.

A cultura do girassol vem se destacando no Brasil e um dos principais motivos para este crescimento é seu uso como matéria-prima no Programa Nacional de Produção e uso do Biodiesel. O objetivo deste trabalho foi avaliar o desempenho produtivo de 12 genótipos de girassol (HLS 01; HLE 11; HELIO 358; EMBRAPA 122; BRSGIRA 01; BRSGIRA 09; SRM 822; TRITON MAX; ZENIT; AGROBEL 960; MG 52 e M 734) na região de Jaguariúna-SP. Os materiais foram avaliados através das seguintes características agronômicas: produtividade (kg.ha-1), produção por capítulo (g), diâmetro do capítulo (m), altura da planta (m) e altura do capítulo (m). Os genótipos apresentaram altos rendimentos (média de 2869 kg ha-1), sem diferirem estatisticamente entre si; o que nos leva a concluir que na região de Jaguariúna - SP há grande potencial de cultivo do girassol, sendo inclusive, uma possível opção para o plantio em sucessão à cana de açúcar.

Desempenho de genótipos de girassol em Jaguariúna - SP - Análise preliminar.

A cultura do girassol vem se destacando no Brasil e um dos principais motivos para este crescimento é seu uso como matéria-prima no Programa Nacional de Produção e uso do Biodiesel. O objetivo deste trabalho foi avaliar o desempenho produtivo de 12 genótipos de girassol (HLS 01; HLE 11; HELIO 358; EMBRAPA 122; BRSGIRA 01; BRSGIRA 09; SRM 822; TRITON MAX; ZENIT; AGROBEL 960; MG 52 e M 734) na região de Jaguariúna-SP. Os materiais foram avaliados através das seguintes características agronômicas: produtividade (kg.ha-1), produção por capítulo (g), diâmetro do capítulo (m), altura da planta (m) e altura do capítulo (m). Os genótipos apresentaram altos rendimentos (média de 2869 kg ha-1), sem diferirem estatisticamente entre si; o que nos leva a concluir que na região de Jaguariúna - SP há grande potencial de cultivo do girassol, sendo inclusive, uma possível opção para o plantio em sucessão à cana de açúcar.

Development of Mass Spectrometry-based analytical tools for characterization of bioconjugate vaccines in E. coli

Antigen design is generally driven by the need to obtain enhanced stability,efficiency and safety in vaccines.Unfortunately,the antigen modification is rarely proceeded in parallel with analytical tools development characterization.The analytical tools set up is required during steps of vaccine manufacturing pipeline,for vaccine production modifications,improvements or regulatory requirements.Despite the relevance of bioconjugate vaccines,robust and consistent analytical tools to evaluate the extent of carrier glycosylation are missing.Bioconjugation is a glycoengineering technology aimed to produce N-glycoprotein in vivo in E.coli cells,based on the PglB-dependent system by C. jejuni,applied for production of several glycoconjugate vaccines.This applicability is due to glycocompetent E. coli ability to produce site-selective glycosylated protein used,after few purification steps, as vaccines able to elicit both humoral and cell-mediate immune-response.Here, S.aureus Hla bioconjugated with CP5 was used to perform rational analytical-driven design of the glycosylation sites for the glycosylation extent quantification by Mass Spectrometry.The aim of the study was to develop a MS-based approach to quantify the glycosylation extent for in-process monitoring of bioconjugate production and for final product characterization.The three designed consensus sequences differ for a single amino-acid residue and fulfill the prerequisites for engineered bioconjugate more appropriate from an analytical perspective.We aimed to achieve an optimal MS detectability of the peptide carrying the consensus sequences,complying with the well-characterized requirements for N-glycosylation by PglB.Hla carrier isoforms,bearing these consensus sequences allowed a recovery of about 20 ng/μg of periplasmic protein glycosylated at 40%.The SRM-MS here developed was successfully applied to evaluate the differential site occupancy when carrier protein present two glycosites.The glycosylation extent in each glycosite was determined and the difference in the isoforms were influenced either by the overall source of protein produced and by the position of glycosite insertion.The analytical driven design of the bioconjugated antigen and the development of accurate,precise and robust analytical method allowed to finely characterize the vaccine.