Light and dark carbon uptake by Dinophysis species in comparison to other photosynthetic and heterotrophic dinoflagellates

The marine dinoflagellate genus Dinophysis includes species that are the causative agents of diarrhetic shellfish poisoning (DSP). Recent findings indicate that some Dinophysis species are mixotrophic, i.e. capable of both autotrophic and heterotrophic nutrition. We investigated inorganic (and organic) carbon uptake by several species of Dinophysis in the Light and dark using the 'single-cell C-14 method', and compared uptake rates with those of photosynthetic Ceratium species and heterotrophic dinoflagellates in the genus Protoperidinium. Experiments were conducted with water from the Gullmar Fjord and from the Koster Strait (Swedish west coast). Nutrient-enriched phytoplankton from surface water samples were concentrated (20 to 70 mu m) and incubated at in situ temperature under artificial light conditions with high concentrations of inorganic C-14 (1 mu Ci ml(-1)). Individual cells of each desired species were manually isolated under a microscope and transferred to scintillation vials. C. tripes showed net C-14 uptake only during light periods, whereas both C. lineatum and C. furca showed C-14 uptake in the Light as well as uptake (and sometimes losses) in the dark. Dinophysis species had similar carbon fixation rates in Light compared to Ceratium species. For D. acuminata and D. norvegica, net carbon uptake occurred in both Light and dark periods. D. acuta showed a loss of carbon in the dark in one experiment, but in another, dark C uptake was significantly higher than uptake in Light. When exposed to Light, C. furca, D. norvegica and D. acuta had high specific carbon uptake rates. Growth rates for the different species were calculated from C-14 uptake by the cells during the first hours of incubation in light. D. acuminata and D. norvegica had similar maximum growth rates, 0.59 and 0.63 d(-1) (mu); the maximum growth rate of D. acuta was lower (0.41 d(-1)). The positive dark carbon uptake by Dinophysis may suggest a mixotrophic mode of nutrition. In one experiment, both D. norvegica and D. acuta showed a significantly higher carbon uptake in a dark bottle than in a Light bottle, which would be consistent with uptake of C-14-labeled organic matter by D. norvegica and D. acuta. Demonstration of direct uptake of dissolved and particulate organic matter would provide conclusive evidence of mixotrophy and this will require the development of new protocols for measuring organic matter uptake applicable to Dinophysis in the natural assemblages.

[A map of the state of Virginia : constructed in conformity to law from the late surveys authorized by the legislature and other original and authentic documents /

Relief shown by hachures. Depths shown by soundings.

A map of the state of Virginia, constructed in conformity to law from the late surveys authorized by the legislature and other original and authentic documents /

Relief shown by hachures. Depths shown by soundings on Drummond's Lake only.

A map of the state of Virginia, constructed in conformity to law from the late surveys authorized by the legislature and other original and authentic documents /

Relief shown by hachures. Depths shown by soundings.

A map of the state of Virginia : constructed in conformity to law from the late surveys authorized by the legislature and other original and authentic documents /

Relief shown by hachures. Depths shown by soundings.

Picasso's Guernica through Sight and Sound

Frederik Kauffman talks about his compositions. Various artists perform his compositions and speak.

Conductors and Insulators

Combined media on photographic paper. 79½" x 56", Jargomatique Series. Summa Corporation Collection

X Factors (Rebecca's Mermaid and Sonja's Plane)

Combined media on photographic paper.

Factors affecting the completion rates of english and humanities courses by technology students.

The purpose of this research was to discover if there was any significant measure of association between a student's family cultural background, primary home language, secondary school language of instruction, high school average and/or English placement level and the likelihood of him or her succeeding in his or her program. Because of both program and demographic differences between «hard» and «soft» technologies, including student population (more specifically gender ratios and student average ages in specific programs), program writing requirements and practical skill program activities, the research was limited to the hard technologies where students work hands-on with hardware and/or computers.||Résumé : L'objectif de cette recherche était de découvrir s'il y avait un lien significatif quelconque entre les origines linguistiques familiales de l'étudiant, ses origines culturelles, sa moyenne au secondaire et/ou ses résultats au test de classement en anglais, et ses chances de compléter ses cours d'anglais et de philosophie dans le délai prescrit de trois ans. Compte tenu des nombreuses différences entre les programmes de formation technique en termes de profil étudiant - sexe et âge en particulier - et d'exigences au niveau de l'écrit et de la pratique, il a été décidé de limiter cette recherche afin d'avoir un échantillon plus uniforme. La recherche porte donc uniquement sur les techniques où l'étudiant est appelé à travailler de façon pratique sur l'ordinateur et où les exigences au niveau de l'écrit et de la recherche sont dans l'ensemble peu élevées.

Perceções de obstáculos à criatividade em universitários de diferentes áreas curriculares e níveis de graduação

As características da atualidade exigem que se seja criativo e inovador. Todo o indivíduo pode rentabilizar intencionalmente seu potencial criativo, podendo e devendo a universidade ter um papel importante nesse desenvolvimento. Porém, observam-se perceções de barreiras à expressão criativa em alunos universitários, apesar de existir muito pouca investigação acerca do tema. Neste estudo, quis-se então analisar perceções de barreiras à criatividade em estudantes universitários portugueses, tomando 582 alunos de três áreas curriculares (Artes e Humanidades; Ciências e Tecnologias; Ciências Sociais e Humanas) e dois níveis de graduação (licenciatura e mestrado). Usou-se o Inventário de Barreiras Pessoais à Expressão Criativa, adaptado à realidade portuguesa. Foram avaliadas diferenças, considerando os fatores do inventário, mas também os seus itens numa análise mais detalhada. Encontraram-se diferenciações nos fatores para a área curricular e, no caso dos itens, para as duas variáveis estudadas. Algumas reflexões são feitas no sentido de repensar práticas no quotidiano académico.

Caldron

Combined media on paper. 96" x 40", Fire River Series. Private Collection

Clinging, Fire

Combined media on paper. 96" x 40", Fire River Series. Private Collection

Darkening of Light

Combined media on paper. 90" x 40", Fire River Series

Gold, Water

Combined media on paper. 82" x 40", Fire River Series. Hammer Museum, UCLA

Keeping Still, Mountain

Combined media on paper. 90" x 40", Fire River Series