Chemical and isotope composition of poor fluid from gas-hydrate samples of ODP Site 146-892

Although the presence of extensive gas hydrate on the Cascadia margin, offshore from the western U.S. and Canada, has been inferred from marine seismic records and pore water chemistry, solid gas hydrate has only been found at one location. At Ocean Drilling Program (ODP) Site 892, offshore from central Oregon, gas hydrate was recovered close to the sediment-water interface at 2-19 m below the seafloor (mbsf) at 670 m water depth. The gas hydrate occurs as elongated platy crystals or crystal aggregates, mostly disseminated irregularly, with higher concentrations occurring in discrete zones, thin layers, and/or veinlets parallel or oblique to the bedding. A 2- to 3-cm thick massive gas hydrate layer, parallel to bedding, was recovered at ~17 mbsf. Gas from a sample of this layer was composed of both CH4 and H2S. This sample is the first mixed-gas hydrate of CH4-H2S documented in ODP; it also contains ethane and minor amounts of CO2. Measured temperatures of the recovered core ranged from 2 to -1.8°C and are 6 to 8 degrees lower than in-situ temperatures. These temperature anomalies were caused by the partial dissociation of the CH4-H2S hydrate during recovery without a pressure core sampler. During this dissociation, toxic levels of H2S (delta34S, +27.4?) were released. The delta13C values of the CH4 in the gas hydrate, -64.5 to -67.5? (PDB), together with deltaD values of -197 to -199? (SMOW) indicate a primarily microbial source for the CH4. The delta18O value of the hydrate H2O is +2.9? (SMOW), comparable with the experimental fractionation factor for sea-ice. The unusual composition (CH4-H2S) and depth distribution (2-19 mbsf) of this gas hydrate indicate mixing between a methane-rich fluid with a pore fluid enriched in sulfide; at this site the former is advecting along an inclined fault into the active sulfate reduction zone. The facts that the CH4-H2S hydrate is primarily confined to the present day active sulfate reduction zone (2-19 mbsf), and that from here down to the BSR depth (19-68 mbsf) the gas hydrate inferred to exist is a >=99% CH4 hydrate, suggest that the mixing of CH4 and H2S is a geologically young process. Because the existence of a mixed CH4-H2S hydrate is indicative of moderate to intense advection of a methane-rich fluid into a near surface active sulfate reduction zone, tectonically active (faulted) margins with organic-rich sediments and moderate to high sedimentation rates are the most likely regions of occurrence. The extension of such a mixed hydrate below the sulfate reduction zone should reflect the time-span of methane advection into the sulfate reduction zone.

Ichthyoplankton density and zooplankton biomass in the northern Benguela upwelling system during D356 September 2010

Ichthyoplankton density (fish eggs and larvae) and bulk zooplankton biomass in September 2010 were determined for 10 stations in the northern Benguela upwelling system, based on oblique Multinet hauls during the RRS Discovery D356 cruise. A HYDROBIOS Multinet, type Midi (0.25 m**2 mouth area) was equipped with five nets of 500 µm-mesh size, temperature and oxygen probes, and an inner and outer flow meter to monitor the net's trajectory (for volume filtered calculations) as well as net clogging. The Multinet was handled over the side, towed horizontally at 2 knots. Winch speed when fearing was 0.5 or 0.3 m/s, heaving velocity 0.2 - 0.3 m/s. The Multinet was towed obliquely at 10 stations sampling the upper 200 m of the water column, which were divided into five different depth strata after inspection of temperature and oxygen concentration depth profiles. Ichthyoplankton densities and zooplankton biomass were calculated for each depth stratum (=single net) from total abundance and the volume of water filtered [individuals per m**3 and g wet weight per m**3, respectively]. In addition, densities and biomass were integrated over the area for each station [individuals per m**2], as sum of calculations for each net: Sum ([individuals per m**3]*Delta(depth bot[m]-depth top [m]).

Ichthyoplankton density and zooplankton biomass in the Benguela upwelling system during AFR258 in December 2009

IIchthyoplankton density (fish eggs and larvae) and bulk zooplankton biomass in December 2009 were determined for 22 stations in the Benguela upwelling system, based on oblique Multinet hauls during the FRS Africana cruise AFR258. A HYDROBIOS Multinet, type Midi (0.25 m**2 mouth area) was equipped with five nets of 500 µm-mesh size, temperature and oxygen probes, and an inner and outer flow meter to monitor the net's trajectory (for volume filtered calculations) as well as net clogging. The Multinet was handled over the side, towed horizontally at 2 knots. Winch speed when fearing was 0.5 or 0.3 m/s, heaving velocity 0.2 - 0.3 m/s. The Multinet was towed obliquely at 22 stations sampling the upper 200 m of the water column, which were divided into five different depth strata after inspection of temperature and oxygen concentration depth profiles. Ichthyoplankton densities and zooplankton biomass were calculated for each depth stratum (=single net) from total abundance and the volume of water filtered [individuals per m**3 and g wet weight per m**3, respectively]. Densities and biomass were integrated over the area for each station [individuals per m**2], as sum of calculations for each net: Sum ([individuals per m**3]*Delta (depth bot[m]-depth top [m]).

Ichthyoplankton density and zooplankton biomass in the Benguela upwelling system during MSM07/3 in March 2008

Ichthyoplankton density (fish eggs and larvae) and bulk zooplankton biomass in March 2008 were determined for 32 stations in the northern Benguela upwelling system, based on oblique Multinet hauls during the FS Maria S. Merian MSM07/3 cruise. A HYDROBIOS Multinet, type Midi (0.25 m**2 mouth area) was equipped with five nets of 500 µm-mesh size, temperature and oxygen probes, and an inner and outer flow meter to monitor the net's trajectory (for volume filtered calculations) as well as net clogging. The Multinet was handled over the side, towed horizontally at 2 knots. Winch speed when fearing was 0.5 or 0.3 m/s, heaving velocity 0.2 - 0.3 m/s. The Multinet was towed obliquely at 32 stations sampling the upper 200 m of the water column, which were divided into five different depth strata after inspection of temperature and oxygen concentration depth profiles. Ichthyoplankton densities and zooplankton biomass were calculated for each depth stratum (=single net) from total abundance and the volume of water filtered [individuals per m**3 and g wet weight per m**3, respectively]. In addition, densities and biomass were integrated over the area for each station [individuals per m**2], as sum of calculations for each net: Sum ([individuals per m**3]*Delta (depth bot[m]-depth top [m]).

Ichthyoplankton density and zooplankton biomass in the Benguela upwelling system during MSM18/4 in July and August 2011

Ichthyoplankton density (fish eggs and larvae) and bulk zooplankton biomass in September 2010 were determined for 10 stations in the northern Benguela upwelling system, based on oblique Multinet hauls during the RRS Discovery D356 cruise. A HYDROBIOS Multinet, type Midi (0.25 m**2 mouth area) was equipped with five nets of 500 µm-mesh size, temperature and oxygen probes, and an inner and outer flow meter to monitor the net's trajectory (for volume filtered calculations) as well as net clogging. The Multinet was handled over the side, towed horizontally at 2 knots. Winch speed when fearing was 0.5 or 0.3 m/s, heaving velocity 0.2 - 0.3 m/s. The Multinet was towed obliquely at 10 stations sampling the upper 200 m of the water column, which were divided into five different depth strata after inspection of temperature and oxygen concentration depth profiles. Ichthyoplankton densities and zooplankton biomass were calculated for each depth stratum (=single net) from total abundance and the volume of water filtered [individuals per m**3 and g wet weight per m**3, respectively]. In addition, densities and biomass were integrated over the area for each station [individuals per m**2], as sum of calculations for each net: Sum ([individuals per m**3]*Delta(depth bot[m]-depth top [m]).

Abundance mesopelagic fish collected during Maria S. Merian cruise MSM26, spring 2013

Mesopelagic fish were collected using a 1 m**2 Double-MOCNESS (Multiple Opening and Closing Net and Environmental Sensing System) and 4.5 m**2 IKMT (Isaacs-Kidd midwater trawl). The main portion of the IKMT was 20 mm knotted nylon, and the tail bag was 3 mm knotless nylon. Oblique IKMT tows were made to a maximum depth of 500 m at a tow speed of 3.5 knots. The original cruise plan intended for nighttime IKMT tows, but tow times varied due to operational constraints. The MOCNESS was equipped with 20 nets of 333 µm mesh size; 10 nets per side. The towing speed was 2 knots. Samples were collected to a maximum depth of 1250 m. The first oblique nets sampled from the surface to the max depth, and the other nets sampled depth stratified bins of the water column. MOCNESS hauls were performed during day and night to investigate diel vertical migrations. Mesoplelagic fish were processed on board. All fish were picked from all IKMT nets, most oblique MOCNESS nets, and the left side nets of the depth stratified MOCNESS samples. The Depth stratified nets from the right side of the MOCNESS frame were preserved in 5 % formalin for future quantitative analyses of the nekton. Fish were identified to the lowest possible taxa using Whitehead et al. (1984) and Fahay (2007). Standard length of each fish was measured to the nearest 0.1 mm using a digital caliper. Measured and identified fish were frozen in an -80 °C freezer, and shipped to the University of Hamburg at the end of the cruise.