Relação entre a resistência a oxacilina e a produção de biofilme de amostras de origem comunitária e hospitalar pertencentes a diferentes espécies de Staphylococcus coagulase negativo

Nas últimas décadas, os Staphylococcus coagulase-negativo, têm sido considerados como patógenos verdadeiros, sendo um dos principais grupos bacterianos responsáveis pelas infecções relacionadas a assistência a saúde (IRAS). O presente estudo teve como objetivo geral: avaliação da relação entre a resistência a oxacilina e a produção de biofilme de amostras Staphylococcus coagulase-negativo de origem comunitária e hospitalar. Neste sentido, foram desenvolvidos os seguintes objetivos específico: identificar ao nível de espécie os Staphylococcus coagulase-negativo; analisar por técnica fenotípica (Ágar vermelho do Congo) a produção de slime; avaliar quantitativamente, a produção de biofilme; correlacionar a produção de polissacarídeos extracelulares (slime) com a produção de biofilme; avaliar a relação da resistência a oxacilina como indicador da presença do gene mecA; avaliar a relação entre a concentração inibitória mínima e a concentração bactericida mínima para oxacilina; pesquisar a presença dos genes mecA, icaAD e atlE, pela técnica de PCR. Foi estudado um total de 150 amostras, sendo 50 isoladas de fômites, 50 isoladas de sangue e 50 isoladas de comunidade. Independente da origem, foram identificadas 14 espécies de Staphylococcus coagulase-negativo, sendo mais frequentes S. epidermidis 42,6%, S. haemolyticus 13,3% e S. cohnii cohnii 10,7%. A análise geral da expressão fenotípica de slime mostrou que 64% das amostras avaliadas eram produtoras de slime. Das 150 amostras testadas neste estudo, 95,3% foram produtoras de biofilme. Ao considerarmos a análise da quantificação do biofilme em relação às origens das amostras estudadas não encontramos diferenças significativas e a maioria das amostras foi considerada moderadamente produtora de biofilme. O gene mecA foi detectado em 6 amostras comunitárias, 34 amostras de fômites e 34 amostras de sangue. Não houve diferença significativa entre as amostras de fômites e sangue. Porém, houve diferença significativa entre as amostras de origem comunitária e as de origem hospitalar - fômites e sangue (p < 0,0001). Ao compararmos as três origens de isolamento quanto a presença do gene atlE observamos que houve diferença significativa (p = 0,0012) entre elas. Sendo, as amostras isoladas de sangue, as que apresentaram maior número de amostras que possuíam o gene atlE (n = 18). Das 150 amostras testadas observamos a presença do gene icaAD em 46% amostras comunitárias, 56% amostras de fômites e 60% amostras de sangue, não encontramos diferença significativa (p = 0,5750). Observamos uma correlação entre a resistência a oxacilina e a produção de slime, pois as amostras de origem hospitalar (fômites e sangue) apresentaram altos níveis de resistência a oxacilina e em sua grande maioria foram produtoras de slime. A espécie S. epidermidis foi a mais isolada e deve-se ressaltar que, quando comparada com as outras espécies, apresentou altos níveis de resistência a oxacilina, sendo a maioria produtora de slime e biofilme.

As tramas sociais do trabalho ambulante e a experiência de trabalhadores em Rio das Ostras/RJ

A presente dissertação se insere no campo de estudo sobre o trabalho informal, tendo como universo empírico de investigação a experiência de trabalho dos trabalhadores ambulantes integrantes do Programa Renda Alternativa, em Rio das Ostras/RJ. Portanto, a pesquisa foi desdobrada em três dimensões: 1) levantamento e estudo bibliográfico; 2) levantamento de dados sobre a formação sócio-histórica da cidade de Rio das Ostras; e, 3) trabalho de campo com ambulantes. Por meio desses dispositivos, a pesquisa buscou compreender os laços entre informalidade do trabalho e dinâmica capitalista, por meio da tradição de estudos das Ciências Sociais nessa área. Seguidamente, a pesquisa se ocupou do estudo de outras investigações sobre trabalho ambulante visando conhecer e ampliar o escopo de análise das peculiaridades desse tipo de trabalho no contexto de reprodução social da chamada superpopulação relativa. Esse universo de questões teóricas, analíticas e empíricas foi confrontado com as especificidades do contexto socioeconômico da cidade de Rio das Ostras, recentemente recomposta como um dos elos da cadeia produtiva do petróleo. Outro eixo da investigação de campo foi conhecer o trabalho, o que foi realizado por meio de entrevistas semi-estruturadas com os trabalhadores ambulantes do município, buscando perceber a relação do trabalhador com o trabalho ao longo de sua vida, as marcas das conjunturas socioeconômicas nas trajetórias e o papel da família e do associativismo na ocupação ambulante. Os achados da pesquisa mostram que o trabalho ambulante acomete gerações de trabalhadores como consequência do desenvolvimento desigual e dependente do capitalismo que limita a oferta de emprego. Somado a isso temos os processos de transformações socioeconômicas das últimas décadas que intensificam as formas de precarização do trabalho potencializando a chamada informalidade do trabalho. Esse processo se intensifica na região em estudo através dos impactos trazidos pelo intenso incremento das indústrias de petróleo e gás que não absorvem a força de trabalho disponível no local e atraída, engendrando uma densa superpopulação relativa para o capital, no município. A experiência de trabalho dos ambulantes que participaram da pesquisa mostra que a mesma é entrecortada por formas laborativas variadas, baixa qualificação profissional, além de dependência dos familiares, de ações públicas do Estado e do associativismo. A expropriação do direito ao emprego empurra os trabalhadores para experiências laborativas inseguras, incertas e sem proteção social. A repetição dessa história entre familiares amplia a percepção da perenidade da expropriação social entre gerações de trabalhadores.

Sendo um consumidor consciente: discursos, projetos e subjetividades

Esta tese analisa construções discursivas sobre o consumidor consciente, o qual visa se diferenciar de um suposto consumo regular e/ou consumista. Toma por base inicial as atribuições propostas em guias e manuais pró consumo consciente, em específico aqueles produzidos pela Akatu e pelo Idec, os quais buscam conceitualizar, debater e promover este tipo de consumidor. Compreende este grupo inicial enquanto uma dimensão prescritiva do discurso os quais elaboram indicações de ação e embasamentos do conceito. O desdobramento do trabalho realiza uma análise qualitativa do discurso dos indivíduos de camadas médias cariocas que se identificam enquanto este tipo de consumidor. Observa-se as semelhanças, diferenças e o desenrolar destes conceitos nas falas de indivíduos em relação àquelas prescritas nos manuais. Assim, pautado em entendimentos sobre consumo enquanto construtor simbólico, noções de projetos de vida e construção de si, produções de sentido e significado, relações de sociabilidade e novas modalidades de participação política, a tese se desenvolve nos seguintes aspectos: análise das bases de origem as quais formulam possibilidades de inserção na temática se ser e fundamentos possíveis; elementos constituintes fundamentais na formação do perfil dos entrevistados e seus conceitos de consciência, os aspectos emotivos sentimentais responsáveis pela valorização ou desvalorização da experiência, e expectativas e perspectivas a respeito dos relacionamentos com terceiros, abordando o tema principalmente através das categorias: chatos e radicais. Com isso, aprofundando-se nas bases definidoras dos sujeitos, suas concepções de consciência, seus medos, anseios, prazer e felicidades; procurando compreender como a subjetividade destes sujeitos está sendo produzida e como dialogam seus projetos com o espaço urbano de uma grande cidade.

Educação a distância na formação pedagógica de preceptores: avaliando a construção de ambientes colaborativos

O Núcleo de Apoio Psicopedagógico ao Residente (NAPPRE) em parceria com a Coordenadoria de Desenvolvimento Acadêmico (CDA) do Hospital Universitário Pedro Ernesto (HUPE) da Universidade do Estado do Rio de Janeiro (UERJ), desenvolve desde 2010 o Curso de Formação Pedagógica para a Prática da Preceptoria (CFPPP) curso de extensão na modalidade aperfeiçoamento, tendo como eixos estruturantes cuidado, educação e gestão. Sustentado nos princípios e diretrizes do Sistema Único de Saúde (SUS) e orientado pelas Diretrizes Curriculares Nacionais de Graduação em Saúde (DCNs), o curso apresenta-se como uma estratégia de Educação Permanente em Saúde (EPS) desta instituição utilizando, tanto nas atividades presenciais como na educação à distância, metodologias ativas para o desenvolvimento da competência pedagógica do preceptor. O projeto político pedagógico do curso valoriza a integração dos profissionais de diferentes categorias para o trabalho em equipe. O alcance dos objetivos educacionais e a construção de projetos de intervenção a serem desenvolvidos nas instituições de origem durante o curso exige interação constante dos participantes e desenvolvimento de espaços colaborativos facilitados por estratégias mistas de educação em atividades presenciais e à distância. Considerando que, aproximadamente, 57% da carga horária do curso corresponde à atividades no ambiente virtual de aprendizagem é fundamental compreender a relevância e funcionalidade deste modelo pedagógico para promover a aprendizagem e induzir práticas colaborativas interdisciplinares compreendidas como um caminho para o desenvolvimento da capacidade criativa, da solidariedade e da inteligência coletiva favorecendo a autonomia crescente, individual e coletiva, a autoria e a produção de cuidado e educação. Estes são elementos constitutivos de práticas educacionais coerentes com as necessidades de formação dos profissionais para qualificar o cuidado em saúde no nosso País. Este projeto de pesquisa tem como objetivo principal avaliar como a Educação à Distância (EaD) favorece a construção de ambientes colaborativos na formação pedagógica de preceptores. Trata-se de um estudo de caso único, retrospectivo, com duas unidades de análise, explanatório, intrínseco e educacional. São integrantes dessa pesquisa, como participantes, todos os preceptores da 1 e 2 turmas (anos 2010 e 2011 respectivamente). O planejamento educacional do curso foi contextualizado considerando as competências pedagógicas que são objetivos de aprendizagem. Foram analisados documentos produzidos de forma individual e coletiva durante as etapas do curso. Os dados quantitativos foram analisados através de estatística descritiva e a análise dos dados coletados no material documental foi por análise de conteúdo segundo as proposições de Bardin com identificação de núcleos e/ou validação destes núcleos através das nuvens de palavras construídas através do wordle.

Threatened corals provide underexplored microbial habitats

Contemporary in-depth sequencing of environmental samples has provided novel insights into microbial community structures, revealing that their diversity had been previously underestimated. Communities in marine environments are commonly composed of a few dominant taxa and a high number of taxonomically diverse, low-abundance organisms. However, studying the roles and genomic information of these “rare” organisms remains challenging, because little is known about their ecological niches and the environmental conditions to which they respond. Given the current threat to coral reef ecosystems, we investigated the potential of corals to provide highly specialized habitats for bacterial taxa including those that are rarely detected or absent in surrounding reef waters. The analysis of more than 350,000 small subunit ribosomal RNA (16S rRNA) sequence tags and almost 2,000 nearly full-length 16S rRNA gene sequences revealed that rare seawater biosphere members are highly abundant or even dominant in diverse Caribbean corals. Closely related corals (in the same genus/family) harbored similar bacterial communities. At higher taxonomic levels, however, the similarities of these communities did not correlate with the phylogenetic relationships among corals, opening novel questions about the evolutionary stability of coral-microbial associations. Large proportions of OTUs (28.7–49.1%) were unique to the coral species of origin. Analysis of the most dominant ribotypes suggests that many uncovered bacterial taxa exist in coral habitats and await future exploration. Our results indicate that coral species, and by extension other animal hosts, act as specialized habitats of otherwise rare microbes in marine ecosystems. Here, deep sequencing provided insights into coral microbiota at an unparalleled resolution and revealed that corals harbor many bacterial taxa previously not known. Given that two of the coral species investigated are listed as threatened under the U.S. Endangered Species Act, our results add an important microbial diversity-based perspective to the significance of conserving coral reefs.

Paracobitis nanpanjiangensis, a new loach (Balitoridae: Nemacheilinae) from Yunnan, China

A new species of the genus Paracobitis, Paracobitis nanpanjiangensis is described from tributaries of the Nanpanjiang River drainage in China. It is distinguished from its congeners, except P. oligolepis and P. wujiangensis, by body scaleless or with rudimentary scales (caudal peduncle with several deeply embedded scales). It can be differentiated from P. wujiangensis by the complete lateral line (vs. incomplete), lower dorsal crest reaching the vertical of origin of anal fin (vs. shorter and higher dorsal crest not reaching the base of anal fin). It is distinguished from P. oligolepis by the following characters: branched dorsal fin with 81/2 (a few 91/2) rays (vs. 91/2), interspaces between bars in front of dorsal fin conspicuously thinner than those behind (vs. vermiform markings), dorsal head without vermiform markings or obscure (vs. clearly vermiform markings on dorsal head), vertebrae 4 + 36-38 (vs. 4 + 39-41).

A duality principle for homogeneous vectorfields with applications

A duality transformation principle was proposed for converting a positive order homogeneous vectorfield into a negative order homogeneous vectorfield. The principle also converted a uniformly locally asymptotically stable differential equation into a uniformly bounded differential equation. The duality transformations included the geometric framework for homogeneity and the removal of origin from the state space.

灵长类高级认知功能起源的遗传机制初探

大约5一7百万年前，从大猿中分出来的一支，走上了以大脑容量快速扩增为特征的演化道路，并最终进化成具有高级认知能力的物种-人。一直以来古人类学家和生物学家经过不懈努力，向我们揭示了人与灵长类其他物种的亲缘关系，并且让我们知道黑猩猩是同人类亲缘关系最近的现生物种。然而人口的爆炸增长和黑猩猩种群数目的急剧减少形成的鲜明对比，让我们迷惑于为何在进化地位上如此相近的物种，命运却截然不同。通过比较两个物种，科学家们发现问题的关键在于：人具有高度发达的大脑和相伴而生的高级认知能力。高度发达的认知能力使得人类获得了对环境强大的适应力，从而能够从众多生物中脱颖而出成为统治地球的智慧生物。通过比较黑猩猩和人基因组序列，生物学家发现人和黑猩猩DNA序列相似度接近99％。这个结果提示我们：1）只有很少数目的基因在一级结构上的变化和认知差异有关；2）认知差异可能更多是由调控相关元件变化导致的基因表达水平上的差异引起的。基于此，我们试图通过比较两个物种目前所知的在人脑中表达的基因的一级结构差异，找寻那些在人起源过程中受到较强正选择的基因。我们检测了近万个基因，结果显示只有大约1．38％的基因有明显的正选择迹象。接着我们从中选取了一个基因-MrgX2，测了全世界范围内的代表性人群以及非人灵长类中的代表性物种，结果显示MrgX2在人这一支受到了很强的适应选择作用，这一适应又很有可能和人类起源过程中感觉神经系统的适应有关。神经生物学的研究表明，大脑皮层前额叶是行使高级认知功能的主要区域。大脑在发育的过程中有一个神经网络重新搭建的过程，而这一过程和基因表达是密切相关的。基于此我们采用基因芯片的方法，通过比较称猴大脑发育各个关键时期的前额叶基因表达图谱，以期了解与大脑神经网络构建相关基因的活动情况。

粗枝云杉( Picea asperata Mast.)天然群体的遗传变异

西南地区在我国的经济发展和生态环境建设中占重要地位，但也是我国生态环境最脆弱的地区之一，生态系统退化，生态功能减弱，严重制约着西南林业的可持续经营与发展。本项目采用DNA 分子标记SSR 研究不同生境条件下粗枝云杉群体的遗传变异及其时空分布格局，考察遗传变异与复杂的山地生态环境间的潜在联系，系统地揭示粗枝云杉天然群体与环境系统相互作用的生态适应与分子进化机制。粗枝云杉适应性强，生长迅速，在植树造林和工业用材方面占有重要地位，研究成果可为中国西南部亚高山天然林的可持续经营及退化生态系统的恢复与重建提供理论依据和科学指导。主要研究结果如下： 1. SSR 位点变异丰富，等位基因频率的分布格局多样。7 个SSR 标记全是多态位点，每位点的等位基因数变化范围为13~24，平均为19.9 个。SSR 位点的等位基因片段长度范围变化较大。73.1%的等位基因变异遵循逐步突变模型(SSM)而发生1 个重复基元的变化，22.3%和4.6%的变异分别按两阶段突变模型(TMP)发生1 个重复基元以上的变化和在SSR 位点侧翼区发生1 个碱基变化的插入-删除事件。 2. 粗枝云杉拥有中等偏高水平的遗传多样性和相对大的群体间遗传分化。通过分析代表10 个群体的250 个个体在7 个SSR位点的变化，调查了源自中国西南山区的粗枝云杉的微卫星变异。相当高的遗传多样性和强烈的群体分化发生在粗枝云杉中， 其群体平均Nei's 期望杂合度为0.707 ， 群体间遗传距离为0.121~0.224(FST)和0.100~0.537(RST)。然而，群体间遗传距离与地理距离之间无相关性，从而排除了简单的距离分离模式并暗示迁移不是影响粗枝云杉遗传变异格局的主要因素。事实上，使用私有等位基因估算的基因流数量非常低，仅等于0.753。等位基因置换检验(Allele permutation tests)揭示逐步突变及遗传漂变都对群体间分化有贡献。另外，在多数位点检测到显著的群体间遗传差异，这个结果说明自然选择，假设通过环境压力，是引起粗枝云杉微地理分化的主要因素之一。根据SSR基因型，250 个粗枝云杉个体的70%被正确地归类入其各自的来源群体，结果表明微卫星(SSR)对区分来自中国不同生态地理位点的粗枝云杉基因型是有效的。 3. 在SSR、RAPD 和AFLP 位点，显著的群体间遗传结构被发现的，但三种标记间遗传分化程度和群体遗传关系有差异。利用来自10 个群体的247 个个体，我们报告了关于样本粗枝云杉群体间遗传关系的总体看法。根据各自对评价遗传关系的信息能力和适用性，SSR、RAPD 和AFLP 标记被选用，三种技术非常有效地区别这些基因型。使用的SSR、RAPD 和AFLP 标记分别估计平均Dice 相似性系数。Mantel 检验产生显著但相对低的共表型适合度(RAPD = 0.63£AFLP = 0.60和SSR = 0.75)。比较三种标记系统，RAPD 和AFLP 共表型指数相对高地相关(r =0.59)，而RAPD 和SSR 及SSR 和AFLP 之间的相关系数分别是0.53 和0.35。所有系统树，包括不同标记资料结合获得的系统树，反映了多数群体依据它们的地理条件而成某种特定关系。结果暗示单个或结合标记系统能用来深入洞察粗枝云杉遗传研究，并且不同标记系统合并资料能提供更可靠的信息。 Southwestern region plays an important role in economic developmentand ecological construction in China. Yet, it is also one of the weak regionsof ecological environment in China with degraded ecosystem and imperfectfunction, which restricts the sustaining management and development ofsouthwestern forestry. The genetic variation and spatial distribution patternof P. asperata populations originating from different habitats wereinvestigated using SSR molecular markers in this study. The correlationsbetween genetic variation and ecological and environmental conditionswere detected, and the interaction between P. asperata populations andenvironmental system and the mechanism of ecological adaption -molecular evolution were revealed. Given the significant ecological andeconomic roles of the fast-growing and wide-adaptive species in reforestation and production of pulp wood and timber, the study couldprovide a strong theoretical evidence and scientific direction for thesustaining management of subalpine natural forest, and the afforestationand rehabilitation of degraded ecosystem. The results are as follows: 1. The genetic variation at SSR loci was abundant and the distributionof allelic frequencies was uneven. All seven loci were polymorphic, and thenumber of alleles per locus varied from 13 to 24 with a mean valueequaling 19.9. The allele sizes at SSR loci were found to vary widely.73.1% of allelic variation followed stepwise mutation model (SSM) whichresults increase or decrease by one repeat type, and 22.3% and 4.6% wereresulted from two-phase mutation model (TMP) with allele size varying bymore than one repeat type and from insertion-deletion events in theflanking regions at SSR loci with a single basepair changing, respectively. 2. P. asperata possessed a moderate to high level of genetic diversityand considerable genetic differentiation. Microsatellite variation of P.asperata. originating from the mountains of southwestern China wasinvestigated by analyzing variation at seven SSR loci in 250 individualsrepresenting ten populations. A fair degree of genetic diversity and strongpopulation subdivision occurred with the mean gene diversity (H) of 0.707,and genetic distances among populations varying between 0.121 and 0.224(FST) and between 0.100 and 0.537 (RST). However, inter-populationgenetic distances showed no correlation with geographic distances between the population sites. This ruled out a simple isolation by distance modeland suggested that migration does not have a great impact. In fact, theamount of gene flow, detected using private alleles, was very low, equalingonly 0.753. Allele permutation tests revealed that stepwise-like mutations,coupled with genetic drift, could contribute to population differentiation.Moreover, significant genetic differences between populations weredetected at most loci. The results indicate that natural selection, presumablythrough environmental stress, may be one of the main factors causingmicro-geographical differentiation in the genetic structure of P. asperata.Based on SSR genotypes, 70% of the 250 individuals were correctlyclassified into their sites of origin. This suggests that microsatellites (SSRs) are effective in distinguishing genotypes of P. asperata originating fromdiverse eco-geographical sites in China. 3. Using a set of 247 individuals from ten P. asperata populations wereport an overview on the genetic relationship among the sampled P.asperata populations. RAPD, AFLP and SSR were used in terms of theirinformativeness and applicability for evaluate relationship and all threetechniques discriminated the genotypes very effectively. Mean Dicesimilarities coefficient were estimated using RAPD, AFLP and SSR,respectively. The Mantel test resulted in a significant but relatively low fit(RAPD = 0.63, AFLP = 0.60 and SSR = 0.75) of cophenetic values.Comparing the three marker systems to each other, RAPD and AFLP cophenetic indices were highly correlated (r = 0.59), while correlationcoefficient between RAPD and SSR was r = 0.53 and between SSR andAFLP was r = 0.35. For all markers a relatively high similarity indendrogram topologies was obtained although some differences wereobserved. All the dendrograms, including that obtained by the combineduse of all the marker data, reflect some relationships for most of thepopulations according to their geographic conditions. The results indicatethat single or combined marker system could be used to insight into geneticstudy in P. asperata and the combined data of different marker systems canprovide more reliable information.

辽河盆地西部凹陷深部流体的地球化学特征与成藏效应

The main research area of this thesis is the Western Depression in the Liaohe Basin. Based on the drilling core observation and mud logging data, the features of the mantle–derived fluids and their effects on oil/gas generation in the Western Depression of the Liaohe Basin,was studied with comprehensive methods of volcanic petrology, sediment petrology, fluid geochemistry, sedimentlogy, and structural geology, and use of polarized light microscope, fluorescence microscope, electron microscope, fluid and melt inclusion test, and isotopic test of nature gas etc. The observation of drill cores in study area and other studies reveal that the main passageway of the volcanic eruption in the Cenozoic was the Xibaqian-Gaosheng fault, and the volcanic rocks of each stage were distributed around it. Mantle-derived fluid which affected on oil/gas generation formed later than the volcanic spew and those fluids entered into the depression through the Taian-Dawa fault and the Central fault. The volatile fraction analysis of the melt inclusion reveals the presence of two kinds of mantle fluids; they are hydrogen-rich fluid and carbon dioxide-rich fluid. These the two kinds of fluids were mainly distributed in olivine and pyroxene respectively. The hydrothermal veins development have multiple stages, from high temperature quartz vein to low temperature calcite vein and analcime vein, in which the fluid inclusion extremity component are methane and carbon dioxide, which indicate that when mantle-derived fluids ascended and entered into the basin, most of these fluids interacted with the organic matter in the basin even though some of these entered into atmosphere. The present isotopic test of the nature gas reveals the high 3He/4He value between the region of the Taian-Dawa fault and the Central fault, which also imply the feature of origin in mantle. This phenomenon indicates that the Mesozoic basement faults and the main Cenozoic faults had connected crust and the mantle during the basin evolution, so the mantle derived fluids could enter the basin along those faults. The main source rocks of the ES3 and ES4 members of the Shahejie Formation began to expel hydrocarbon at the end period of the ES1 member of the Shahejie Formation, and reached its peak during the period of the Dongying Formation deposition. During these periods, the mantle derived-fluids entered the basin constantly along the main faults, and supplied lots of hydrogen for hydrocarbon generation. Though the volcanic rocks and the mantle-derived fluids in the Eastern Depression were more developed than in the Western Depression, the source rocks and the deep fluids were not interacted better than the Western Depression because of the affection of structural evolution. In the Eocene, the Eastern Depression did not deposit the ES4 member of the Shahejie Formation, furthermore, the mantle-fluid formed in the Fangshengpao stage escaped to the atmosphere, which confined the later stage hydrocarbon generation capability.

柴西南区下干柴沟组下段层序地层格架内沉积相展布及有利储集砂体分布研究

Lower member of the lower Ganchaigou Formation in the southwestern of Qaidam Basin is one of the main targeted exploration zones. With the advancement of exploration, the targets are gradually switching into the lithologic reservoirs and it is urgent to gain the more precise research results in distribution of sedimentary facies and sandstones. Guided by the theory of sequence stratigraphy and sedimentology as well as on the basis of many logging data, drillings, seismic data and chemical tests, the paper comprehensively analyzes the sedimentary facies and sandstones in the lower member of lower Ganchaigou Formation in the southern of Chaixi. According to the identification marks of the key interface in sequence stratigraphy, the key interfaces in lower member of lower Ganchaigou Formation in the southwestern of Qaidam Basin are identified as two third-order sequences SQ1、SQ2. By calibrating the synthetic seismogram, the seismic sequence, well drilling and logging sequences are united. Based on the works above, this paper chooses seven primary cross-sections and builds connecting-well stratigraphic correlation of seven main connecting-well sections. Ultimately, the high-resolution sequence stratigraphic frameworks in the lower member of the lower Ganchaigou Formation, which are uniform to logging and seismic data, are figured out. In terms of study on each sequence features, the main style of the base-level cycle overlay which forms the third-order sequence is confirmed. It contains asymmetric “becoming deep upward” style and symmetry style. Researching on the spreading characters of sequence stratigraphy indicates that SQ1 and SQ2 are rather thicker near northwest well Shashen 20 and Shaxin1 while they are quite thiner near Hongcan 1, Yuejin, Qie 4 and Dong8-Wu3, and the thickness of SQ1 is thicker than SQ2.Based on the deep analysis of the marks for depositional facies, it is proposed that the lake facies and braid river deltas facies mainly occurred in study areas. Besides, the sorts of sub-facies and micro-facies model are divided and described. Under the control of high-resolution sequence stratigraphic framework, three source directions from Arlarer Mountain、Qimantage Mountain and Dongchai Mountain are identified by using the features of heavy mineral assemblage and paleogeomorphy. In addition, regularities of distribution sedimentary facies in sequence stratigraphic framework are studied in accordance with research thinking of the "point" (single well) "line" (section) "face" (plane). In the stage of lower member in the lower Ganchaigou Formation in the southwestern of Qaidam Basin, it is at the early phrase of evolution of the lake basin with the gradual outspread and the rise of the lake level. Combined with physical analysis of reservoir sands formed in different sedimentary environment, the paper studies the style of favorable sandstone bodies that are underwater distributary channel of braided rive delta front, coarse sand in mouth bar and the sand body in sand flat of shore-shallow lacustrine facies. Finally, this article comprehensively analyzes the distribution relationship between sedimentary facies and favorable sandstone body and proposes the ideas that sequence SQ1 Yuejin area, well east 8-wu3 area, well qie4-qie1 area and well hongcan2 area are distributed areas of favorable sandstone.

断层封闭性及其与油气成藏的关系研究-以沾化凹陷孤岛地区为例

Godao area, located in the east of the Zhanhua depression of Jirang sag in Bohai Bay Basin, is the studying area in my dissertation. It is first time that fault sealing properties and the related relationship with the pool forming are studied in Gudao area. On the base of the analysis of the regional tectonics, the author has studied the tertiary structural evolution of the Gudao area and distinguished the fault's level and put forward the distinguishing principle. The geometrical feature, mechanical characters, developmental mechanism and history of the boundary faults in the tectonic unit of this area are all studied and emphasized especially. The buried history of oil-generating depression (that is Gudao depression) and the history of oil and gas migration simultaneously are discussed, the juxtaposition relationship between boundary fault evolutionary history and oil and gas migrated history are expatiated. To the geological condition of the Gudao area, three level faults sealing properties of this area were discussed in detail. Their characteristics of behavior and the intrinsic relationship between their sealing and oil and gas migrated reservoir are elucidated. The pool-forming models related to fault seal are exposed. The author has studied the lithologies of different order of faults, the relationship of occurrence assemblage analysis, normal stress of fault plane in different depth and shale smear factor faults. Then analysis their role in the various faults sealing and confirms fault sealing marks of three different orders faults and exposes the mechanism of fault sealing. Shale smear zone formed by first order fault in lasting activities is one different type of fault breccia and mainly controlled factor to its entrapment of petroleum. Effective sealing threshold value and fault displacement is ascertained. Mainly controlled factor of second fault sealing is bigger compressive stress loaded on fault plane. According to this, quantitatively evaluated index is given. Shale smear zone is necessary condition for second fault stress entrapment. The juxtaposed relationship between the different lithologies within third order faults is most important controlled factor for its sealing. Based on various order of fault sealing features and mechanism in Gudao area, the author proposed three orders of fault sealing models. Shale smear zone sealing model, normal stress sealing model and lithologies juxtaposed sealing model are suggested to first, second and third order fault respectively. The conclusion of this studying has not only the very important theoretical significance and practical value in Gudo area but also the very important guiding role for other areas of related aspects.

心理咨询师的心理需要及相关因素的研究

The aim of this research is to explore the tendency of needs and its influential factors in counselors at present time．Three studies were carried out: study 1 was to find out the tendency of needs and the structure of needs in counselors. Study 2 was to compare the needs between counselors and non-counselors, in which 64 subjects were students majored in psychology but they don’t want to counsel.Study 3 was to initially explore the relationships between the tendency of needs and its influential factors. Studies of the 1 and 3 selected 123 counselors. It was found that: 1. The results of EPPS in counselors: according to comparison between original marks, the number of subjects who range first from high to low was: introspection，change，dominance，nurse，order，succor，affinity=autonomy，achievement，endurance，heterosexuality，deference，aggression=exhibition=abasement ; according to means of original marks, the tendency of needs from high to low was: introspection，achievement，nurse，autonomy，change，succor，endurance，dominance，order，affinity，aggression，exhibition，deference，abasement，heterosexuality. The lower one of the counselors were needs of aggression, exhibition，deference，abasement and heterosexuality，the high one are introspection，nurse and change．The most difference was achievement need. 2. Dilemmas had no significantly different effect on the needs of counselors, but sexuality and age affect them in some factors. There were significant differences in the factors of deference, exhibition and nurse among different ages. Counselors married had higher tendency of obedience, autonomy, introspection, nurse and endurance than those unmarried. 3. The results of EPPS in non-counselors: according to comparison between original marks, the number of subjects who range first from high to low was: endurance，order，change=dominance，autonomy= introspection=nurse，affinity，succor，aggression，achievement，heterosexuality，exhibition=abasement，deference; according to original marks’means, the tendency of needs from high to low was: nurse，endurance，achievement，order，introspection，change，autonomy，dominance，affinity，succor，aggression，exhibition，abasement，deference，heterosexuality. The lower one of the counselors were needs of aggression, exhibition，deference，abasement and heterosexuality，the high one were introspection，nurse and change．The most difference was achievement need. 4. There were significant differences in the needs of abasement and introspection between counselors and non-counselors. Male counselors and non-counselors had significant differences in the factor of abasement. Female counselors had higher tendency of succor needs. 5. The differences of childhood traumas and positive life events were not significant. The positive and negative life events themselves had no significant differences, but negative life events had marginal significant difference between the two groups. 6. The marks of counselors were lower than non-counselors in the factors of neuroticism. 7. Counselors and trainees had no significant differences among other needs、childhood traumas、life events、personality and coping styles except in the deference need. 8. The result of multiple estimation of EPQ , CTQ and EPPS were related．Many specific life events were related with the tendency of many needs. As shown in the research, children’s traumas, characteristic and life events may affect needs chiefly. 9. There were close relation between characteristic and childhood traumas in counselors. Especially the subjects with more childhood traumas had higher tendency of psychoticism, neuroticism and introvision. There were few persons with much high scores on the Childhood traumas in the subjects．Their personality characteristics indicate high extrovision and low psychoticism．Their childhood traumas may affect the shape of sound personality． Conclusion: 1. The most important tendency of needs were introspection，nurse and change． 2. The differences of childhood traumas and life events were not significant between counselors and non-counselors. The marks of counselors were higher than non-counselors in the factors of introspection, but lower in the factor of neuroticism． 3. The main influential factors in counselors of needs were childhood traumas、characteristic and life events.

International retail divestment activity

Alexander, N.; Quinn, B.; and Cairns, P. (2005). International retail divestment activity. International Journal of Retail and Distribution Management. 33(1), pp.5-22 RAE2008

Rola stereotypów w komunikacji międzykulturowej biznesowej: studium porównawcze polsko-ukraińskie

Wydział Neofilologia: Katedra Ekokomunikacji