The ATPase and protease domains of yeast mitochondrial Lon: Roles in proteolysis and respiration-dependent growth

The ATP-dependent Lon protease of Saccharomyces cerevisiae mitochondria is required for selective proteolysis in the matrix, maintenance of mitochondrial DNA, and respiration-dependent growth. Lon may also possess a chaperone-like function that facilitates protein degradation and protein-complex assembly. To understand the influence of Lon’s ATPase and protease activities on these functions, we examined several Lon mutants for their ability to complement defects of Lon-deleted yeast cells. We also developed a rapid procedure for purifying yeast Lon to homogeneity to study the enzyme’s activities and oligomeric state. A point mutation in either the ATPase or the protease site strongly inhibited the corresponding activity of the pure protein but did not alter the protein’s oligomerization; when expressed at normal low levels neither of these mutant enzymes supported respiration-dependent growth of Lon-deleted cells. When the ATPase- or the protease-containing regions of Lon were expressed as separate truncated proteins, neither could support respiration-dependent growth of Lon-deleted cells; however, coexpression of these two separated regions sustained wild-type growth. These results suggest that yeast Lon contains two catalytic domains that can interact with one another even as separate proteins, and that both are essential for the different functions of Lon.

The ordinances of the Clothworkers' Company together with those of the ancient guilds or fraternities of the fullers & shearmen of the city of Lon don, transcribed from the originals in the possession of the Company.

Includes index.

The public prosecutor of the terror, Antoine Quentin Fouquier-Tinville;

Mode of access: Internet.

Histoire du ciel : ou lon recherche l'origine de lidolatrie et les méprises de la philosophie, sur la formation des corps célestes & de toute la nature : Tome second /

Mode of access: Internet.

Histoire du ciel : ou lon recherche l'origine de lidolatrie et les méprises de la philosophie, sur la formation des corps célestes & de toute la nature : Tome premier /

Mode of access: Internet.

Biographie médicale par ordre chronologique d'après Daniel Leclerc, Éloy, etc.... /

Texto a dos col.

The public prosecutor of the terror, Antoine Quentin Fouquier-Tinville;

Mode of access: Internet.

Memoirs of the life and works of Jean Antoine Houdon, the sculptor of Voltaire and of Washington,

Mode of access: Internet.

A testimony from the Two Months' Meeting of Congénies : of those who profess with Friends, concerning Louis Antoine Majolier, who died at Congénies the sixth of 3rdmonth, 1842. Read in the Yearly Meeting of 1844.

Smith II:724.

Vista del Cabo San Antonio [Material gráfico] = Vue du Cap St. Antoine = View of the cape of San Antonio

Estampada junto a: "Detalles y cortes de un sepulcro en Dayemus"

Characterising the homeostatic and hedonic markers of the susceptible phenotype

Over the years, approaches to obesity prevention and treatment have gone from focusing on genetic and other biological factors to exploring a diversity of diets and individual behavior modification interventions anchored primarily in the power of the mind, to the recent shift focusing on societal interventions to design ";temptation-proof"; physical, social, and economic environments. In spite of repeated calls to action, including those of the World Health Organization (WHO), the pandemic continues to progress. WHO recently projected that if the current lifestyle trend in young and adult populations around the world persist, by 2012 in countries like the USA, health care costs may amount to as much as 17.7% of the GDP. Most importantly, in large part due to the problems of obesity, those children may be the first generation ever to have a shorter life expectancy than that of their parents. This work presents the most current research and proposals for addressing the pandemic. Past studies have focused primarly on either genetic or behavioral causes for obesity, however today's research indicates that a strongly integrated program is the best prospect for success in overcoming obesity. Furthermore, focus on the role of society in establishing an affordable, accessible and sustainable program for implementing these lifestyle changes is vital, particularly for those in economically challenged situations, who are ultimately at the highest risk for obesity. Using studies from both neuroscience and behavioral science to present a comprehensive overview of the challenges and possible solutions, The brain-to-society approach to obesity prevention focuses on what is needed in order to sustain a healthy, pleasurable and affordable lifestyle.