Space-borne study of seasonal, multi-year, and decadal phytoplankton dynamics in the Bay of Biscay

Seasonal and inter-annual variations in phytoplankton community abundance in the Bay of Biscay are studied. Preliminarily processed by the National Aeronautics and Space Administration (NASA) to yield normalized water-leaving radiance and the top-of-the-atmosphere solar radiance, Sea-viewing Wide Field-of-View Sensor (SeaWiFS), Moderate Resolution Imaging Spectroradiometer (MODIS), and Coastal Zone Color Scanner (CZCS) data are further supplied to our dedicated retrieval algorithms to infer the sought for parameters. By applying the National Oceanic and Atmospheric Administration's (NOAA's) Advanced Very High Resolution Radiometer (AVHRR) data, the surface reflection coefficient in the only band in the visible spectrum is derived and employed for analysis. Decadal bridged time series of variations of diatom-dominated phytoplankton and green dinoflagellate Lepidodinium chlorophorum within the shelf zone and the coccolithophore Emiliania huxleyi in the pelagic area of the Bay are documented and analysed in terms of impacts of some biogeochemical and geophysical forcing factors.

The biodiscovery potential of marine bacteria: an investigation of phylogeny and function

A collection of marine bacteria isolated from a temperate coastal zone has been screened in a programme of biodiscovery. A total of 34 enzymes with biotechnological potential were screened in 374 isolates of marine bacteria. Only two enzymes were found in all isolates while the majority of enzyme activities were present in a smaller proportion of the isolates. A cluster analysis demonstrated no significant correlation between taxonomy and enzyme function. However, there was evidence of co-occurrence of some enzyme activity in the same isolate. In this study marine Proteobacteria had a higher complement of enzymes with biodiscovery potential than Actinobacteria; this contrasts with the terrestrial environment where the Actinobacteria phylum is a proven source of enzymes with important industrial applications. In addition, a number of novel enzyme functions were more abundant in this marine culture collection than would be expected on the basis of knowledge from terrestrial bacteria. There is a strong case for future investigation of marine bacteria as a source for biodiscovery.

From silk to satellite: half a century of ocean colour anomalies in the Northeast Atlantic

Changes in phytoplankton dynamics influence marine biogeochemical cycles, climate processes, and food webs, with substantial social and economic consequences. Large-scale estimation of phytoplankton biomass was possible via ocean colour measurements from two remote sensing satellites – the Coastal Zone Color Scanner (CZCS, 1979-1986) and the Sea-viewing Wide Field-of-view Sensor (SeaWiFS, 1998-2010). Due to the large gap between the two satellite eras and differences in sensor characteristics, comparison of the absolute values retrieved from the two instruments remains challenging. Using a unique in situ ocean colour dataset that spans more than half a century, the two satellite-derived chlorophyll-a (Chl-a) eras are linked to assess concurrent changes in phytoplankton variability and bloom timing over the Northeast Atlantic Ocean and North Sea. Results from this unique re-analysis reflect a clear increasing pattern of Chl-a, a merging of the two seasonal phytoplankton blooms producing a longer growing season and higher seasonal biomass, since the mid-1980s. The broader climate plays a key role in Chl-a variability as the ocean colour anomalies parallel the oscillations of the Northern Hemisphere Temperature (NHT) since 1948.

Ecohydrodynamics of cold-water coral reefs: A case study of the Mingulay Reef complex (western Scotland)

Ecohydrodynamics investigates the hydrodynamic constraints on ecosystems across different temporal and spatial scales. Ecohydrodynamics play a pivotal role in the structure and functioning of marine ecosystems, however the lack of integrated complex flow models for deep-water ecosystems beyond the coastal zone prevents further synthesis in these settings. We present a hydrodynamic model for one of Earth's most biologically diverse deep-water ecosystems, cold-water coral reefs. The Mingulay Reef Complex (western Scotland) is an inshore seascape of cold-water coral reefs formed by the scleractinian coral Lophelia pertusa. We applied single-image edge detection and composite front maps using satellite remote sensing, to detect oceanographic fronts and peaks of chlorophyll a values that likely affect food supply to corals and other suspension-feeding fauna. We also present a high resolution 3D ocean model to incorporate salient aspects of the regional and local oceanography. Model validation using in situ current speed, direction and sea elevation data confirmed the model's realistic representation of spatial and temporal aspects of circulation at the reef complex including a tidally driven current regime, eddies, and downwelling phenomena. This novel combination of 3D hydrodynamic modelling and remote sensing in deep-water ecosystems improves our understanding of the temporal and spatial scales of ecological processes occurring in marine systems. The modelled information has been integrated into a 3D GIS, providing a user interface for visualization and interrogation of results that allows wider ecological application of the model and that can provide valuable input for marine biodiversity and conservation applications.

The co-location of offshore windfarms and decapod fisheries in the UK: Constraints and opportunities

The offshore wind sector in the UK is expanding rapidly and is set to occupy significant areas of the coastal zone, making it necessary to explore the potential for co-location with other economic activities. The presence of turbine foundations introduces hard substrates into areas previously dominated by soft sediments, implying that artificial reef effects may occur, with potential benefits for fisheries. This review focuses on the possibilities for locating fisheries for two commercially important decapods, the brown crab Cancer pagurus and the European lobster Homarus gammarus, within offshore wind farms. Existing understanding of habitat use by C pagurus and H. gammarus suggests that turbine foundations have the potential to act as artificial reefs, although the responses of these species to noise and electromagnetic fields are poorly understood. Offshore wind farm monitoring programmes provide very limited information, but do suggest that adult C pagurus associate with turbine foundations, which may also serve as nursery areas. There was insufficient deployment and monitoring of rock armouring to draw conclusions about the association of H. gammarus with offshore wind farm foundations. The limited information currently available demonstrates the need for further research into the ecological and socioeconomic issues surrounding fishery co-location potential.

Improving clinician self-efficacy does not increase asthma guideline use by primary care clinicians

Objective: The purpose of this study was to show the association between changes in clinician self-efficacy and readiness to change and implementation of an asthma management program (Easy Breathing). Methods: A 36 month randomized, controlled trial was conducted involving 24 pediatric practices (88 clinicians). Randomized clinicians received interventions designed to enhance clinician self-efficacy and readiness to change which were measured at baseline and 3 years. Interventions consisted of an educational toolbox, seminars, teleconferences, mini-fellowships, opinion leader visits, clinician-specific feedback, and pay for performance. The primary outcome was program utilization (number of children enrolled in Easy Breathing/year); secondary outcomes included development of a written treatment plan and severity-appropriate therapy. Results: At baseline, clinicians enrolled 149 ± 147 (mean ± SD) children/clinician/year; 84% of children had a written treatment plan and 77% of plans used severity-appropriate therapy. At baseline, higher self-efficacy scores were associated with greater program utilization (relative rate [RR], 1.34; 95% confidence interval [CI], 1.04-1.72; P =.04) but not treatment plan development (RR, 0.63; 95% CI, 0.29-1.35; P =.23) or anti-inflammatory use (RR, 1.76; 95% CI, 0.92-3.35; P =.09). Intervention clinicians participated in 17 interventions over 36 months. At study end, self-efficacy scores increased in intervention clinicians compared to control clinicians (P =.01) and more clinicians were in an action stage of change (P =.001) but these changes were not associated with changes in primary or secondary outcomes. Conclusions: Self-efficacy scores correlated with program use at baseline and increased in the intervention arm, but these increases were not associated with greater program-related activities. Self-efficacy may be necessary but not sufficient for behavior change. Copyright © 2012 by Academic Pediatric Association.

The buffering effect of hope on clinicians' behavior:A test in pediatric primary care

Although trait hope is thought to motivate goal-directed actions in the face of impediments, few studies have directly examined hope's role in overcoming obstacles, and none have done so while accounting for related goal constructs. We describe a study of 127 pediatric primary care providers who over the course of a year were asked to identify new cases of asthma and confirm previously diagnosed active disease by completing for each of their patients a brief survey validated for this purpose. These clinicians also completed measures of hope, self-efficacy, conscientiousness, and perceived obstacles to implementing a pediatric asthma management program. As predicted by hope theory, the agency component of hope buffered clinicians from perceived obstacles by facilitating the identification of asthma cases among high-hope clinicians in the face of obstacles. This buffering effect remained after controlling for self-efficacy and conscientiousness. We discuss the study findings in terms of current theories of goal-directed behavior and implications for delivering hope-related interventions, and we offer a testable hypothesis regarding when agency and pathways thinking facilitate goal-related behavior.

Nearshore oscillating wave surge converters and the development of Oyster

Oscillating wave surge converters (OWSCs) are a class of wave power technology that exploits the enhanced horizontal fluid particle movement of waves in the nearshore coastal zone with water depths of 10–20 m. OWSCs predominantly oscillate horizontally in surge as opposed to the majority of wave devices, which oscillate vertically in heave and usually are deployed in deeper water. The characteristics of the nearshore wave resource are described along with the hydrodynamics of OWSCs. The variables in the OWSC design space are discussed together with a presentation of some of their effects on capture width, frequency bandwidth response and power take-off characteristics. There are notable differences between the different OWSCs under development worldwide, and these are highlighted. The final section of the paper describes Aquamarine Power’s 315kW Oyster 1 prototype, which was deployed at the European Marine Energy Centre in August 2009. Its place in the OWSC design space is described along with the practical experience gained. This has led to the design of Oyster 2, which was deployed in August 2011. It is concluded that nearshore OWSCs are serious contenders in the mix of wave power technologies. The nearshore wave climate has a narrower directional spread than the offshore, the largest waves are filtered out and the exploitable resource is typically only 10–20% less in 10m depth compared with 50m depth. Regarding the devices, a key conclusion is that OWSCs such as Oyster primarily respond in the working frequency range to the horizontal fluid acceleration; Oyster is not a drag device responding to horizontal fluid velocity. The hydrodynamics of Oyster is dominated by inertia with added inertia being a very significant contributor. It is unlikely that individual flap modules will exceed 1MW in installed capacity owing to wave resource, hydrodynamic and economic constraints. Generating stations will be made up of line arrays of flaps with communal secondary power conversion every 5–10 units.

Understanding macroalgal dispersal in a complex hydrodynamic environment: a combined population genetic and physical modelling approach

Gene flow in macroalgal populations can be strongly influenced by spore or gamete dispersal. This, in turn, is influenced by a convolution of the effects of current flow and specific plant reproductive strategies. Although several studies have demonstrated genetic variability in macroalgal populations over a wide range of spatial scales, the associated current data have generally been poorly resolved spatially and temporally. In this study, we used a combination of population genetic analyses and high-resolution hydrodynamic modelling to investigate potential connectivity between populations of the kelp Laminaria digitata in the Strangford Narrows, a narrow channel characterized by strong currents linking the large semi-enclosed sea lough, Strangford Lough, to the Irish Sea. Levels of genetic structuring based on six microsatellite markers were very low, indicating high levels of gene flow and a pattern of isolation-by-distance, where populations are more likely to exchange migrants with geographically proximal populations, but with occasional long-distance dispersal. This was confirmed by the particle tracking model, which showed that, while the majority of spores settle near the release site, there is potential for dispersal over several kilometres. This combined population genetic and modelling approach suggests that the complex hydrodynamic environment at the entrance to Strangford Lough can facilitate dispersal on a scale exceeding that proposed for L. digitata in particular, and the majority of macroalgae in general. The study demonstrates the potential of integrated physical–biological approaches for the prediction of ecological changes resulting from factors such as anthropogenically induced coastal zone changes.

Assessment of a zoomed global model for the North Sea by comparison with a conventional nested regional model

Assessment of marine downscaling of global model simulations to the regional scale is a prerequisite for understanding ocean feedback to the atmosphere in regional climate downscaling. Major difficulties arise from the coarse grid resolution of global models, which cannot provide sufficiently accurate boundary values for the regional model. In this study, we first setup a stretched global model (MPIOM) to focus on the North Sea by shifting poles. Second, a regional model (HAMSOM) was performed with higher resolution, while the open boundary values were provided by the stretched global model. In general, the sea surface temperatures (SSTs) in the two experiments are similar. Major SST differences are found in coastal regions (root mean square difference of SST is reaching up to 2°C). The higher sea surface salinity in coastal regions in the global model indicates the general limitation of this global model and its configuration (surface layer thickness is 16 m). By comparison, the advantage of the absence of open lateral boundaries in the global model can be demonstrated, in particular for the transition region between the North Sea and Baltic Sea. On long timescales, the North Atlantic Current (NAC) inflow through the northern boundary correlates well between both model simulations (R~0.9). After downscaling with HAMSOM, the NAC inflow through the northern boundary decreases by ~10%, but the circulation in the Skagerrak is stronger in HAMSOM. The circulation patterns of both models are similar in the northern North Sea. The comparison suggests that the stretched global model system is a suitable tool for long-term free climate model simulations, and the only limitations occur in coastal regions. Regarding the regional studies focusing on the coastal zone, nested regional model can be a helpful alternative.

Two-Year Monitoring of Water Samples from Dam of Iskar and the Black Sea, Bulgaria, by Molecular Analysis: Focus on Mycobacterium spp

The coast of the Bulgarian Black Sea is a popular summer holiday destination. The Dam of Iskar is the largest artificial dam in Bulgaria, with a capacity of 675 million m3. It is the main source of tap water for the capital Sofia and for irrigating the surrounding valley. There is a close relationship between the quality of aquatic ecosystems and human health as many infections are waterborne. Rapid molecular methods for the analysis of highly pathogenic bacteria have been developed for monitoring quality. Mycobacterial species can be isolated from waste, surface, recreational, ground and tap waters and human pathogenicity of nontuberculose mycobacteria (NTM) is well recognized. The objective of our study was to perform molecular analysis for key-pathogens, with a focus on mycobacteria, in water samples collected from the Black Sea and the Dam of Iskar. In a two year period, 38 water samples were collected-24 from the Dam of Iskar and 14 from the Black Sea coastal zone. Fifty liter water samples were concentrated by ultrafiltration. Molecular analysis for 15 pathogens, including all species of genus Mycobacterium was performed. Our results showed presence of Vibrio spp. in the Black Sea. Rotavirus A was also identified in four samples from the Dam of Iskar. Toxigenic Escherichia coli was present in both locations, based on markers for stx1 and stx2 genes. No detectable amounts of Cryptosporidium were detected in either location using immunomagnetic separation and fluorescence microscopy. Furthermore, mass spectrometry analyses did not detect key cyanobacterial toxins. On the basis of the results obtained we can conclude that for the period 2012-2014 no Mycobacterium species were present in the water samples. During the study period no cases of waterborne infections were reported.

Turismo sustentável nas zonas costeiras e o caso das praias de Mira

As zonas costeiras desde cedo foram consideradas como áreas preferenciais e com vocação para a prática de actividades turísticas. Por outro lado, o sector do turismo tem sido apontado como um factor de desenvolvimento, sobretudo para países ou regiões onde o tecido económico e produtivo é pouco competitivo, apresentando-se esta actividade como um sector chave para o desenvolvimento (Relatório do Estado do Ambiente, 1999). No entanto, este sector tem levantado alguns problemas ambientais nas zonas costeiras, pelo excessivo consumo do produto turístico sol e mar. Além disso, os turistas de hoje já não procuram apenas estes produtos turísticos, mas também estão interessados em que estas áreas possuam outros produtos turísticos de qualidade, tanto a nível ambiental como a nível cultural. Apareceu, depois da conferência mundial sobre desenvolvimento e ambiente, o conceito de turismo sustentável, como parte integrante do desenvolvimento sustentável, o que levou a que se procedesse à criação de critérios para a prática deste tipo de turismo. A nível europeu está a ser elaborada uma Agenda 21 para o sector do turismo, definindo as linhas orientadoras para este sector, de forma a satisfazer as necessidades dos visitantes, mas também de forma a preservar e melhorar a herança cultural e ambiental dos territórios com potencialidades turísticas. Estas linhas orientadoras deverão ser também aplicadas de forma concreta às zonas costeiras onde se verifica cada vez mais uma degradação do ambiente, e consequentemente do sector turístico. Da verificação destes problemas entre o sector do turismo e as áreas costeiras, procedeu-se ao estudo da noção de Turismo Sustentável e/ou Sustentabilidade do Turismo nas zonas costeiras, principalmente naquelas que necessitam de um rejuvenescimento turístico e ambiental. Foi analisado o caso de estudo do concelho de Mira, com o intuito de verificar como poderão ser aplicados os critérios da sustentabilidade do turismo neste território que necessita ser rejuvenescido. Pretende apresentar-se um programa de turismo sustentável em que sejam apontadas as várias potencialidades do concelho de Mira para tornar o sector do turismo mais sustentável, diversificando o produto turístico da região, oferecendo novas e renovadas condições aos seus visitantes, conservando e promovendo os recursos naturais e culturais do concelho e criando benefícios e oportunidades à sua população.

E-assessment em ensino a distância on-line: estudo exploratório no curso de licenciatura em gestão de negócios da Universidade Eduardo Mondlane

A evolução acelerada da tecnologia, com um grande impacto em quase todos os segmentos da humanidade, coloca o homem perante novos desafios, com vários graus de complexidade. Cada dia que passa, estes desafios exigem novas competências e estratégias que devem ser adequadas a cada contexto. A dinâmica das tecnologias de comunicação e informação impulsionaram a evolução do ensino a distância (EaD), o que de certo modo tem exigido grandemente às instituições provedoras do EaD em geral e, em particular, às provedoras do EaD on-line novas formas de gestão, e novas formas de ensinar, fazer aprender os seus estudantes e avaliar as suas aprendizagens. No sentido de contribuir para a evolução e melhoramento do processo de avaliação das aprendizagens on-line, desenvolveu-se um estudo sobre o tema avaliação das aprendizagens on-line como elemento potenciador da interação em EaD. O desenvolvimento do tema foi orientado por um referencial teórico e contextual e concretizou-se, na sua vertente empírica, num estudo de caso exploratório. Este estudo desenvolveu-se em duas fases. Fase I: de caracterização de conceções e “práticas” vigentes de avaliação das aprendizagens on-line no Curso de licenciatura de Gestão de Negócios (LGN) da Universidade Eduardo Mondlane (UEM) de Moçambique; Fase II: de conceção, implementação e avaliação de uma proposta alternativa de avaliação das aprendizagens on-line no Curso de LGN. Para a consecução do estudo empírico concorreram um conjunto de procedimentos metodológicos que envolveu diversas técnicas e instrumentos de recolha de dados. Na fase I recorreu-se à entrevista a seis atores do Curso de LGN (3 professores, 3 assessores pedagógicos e 3 estudantes) e à análise documental (documentos relativos à conceção do Curso e outros relativos à sua implementação). Na fase II recorreu-se à observação participante, a um questionário respondido pelos estudantes participantes, a um relatório elaborado pelo professor colaborador na implementação da proposta por nós concebido, e a documentos retirados da plataforma usada no Curso de LGN, a plataforma Aulanet, nomeadamente quanto às interações dos estudantes em fóruns e chats. Os dados recolhidos foram sujeitos a análises quantitativas e qualitativas.Dos resultados obtidos na Fase I realça-se a necessidade sentida pelos entrevistados de se mudarem práticas de avaliação on-line, nomeadamente de forma a potenciar a interação e interatividade e permitir que a classificação nos módulos não fosse apenas resultado de um exame final. Dos resultados obtidos na Fase II enfatiza-se a apreciação globalmente positiva da nova metodologia de avaliação das aprendizagens on-line, quer pelos estudantes, quer pelo professor participante. Foram identificados como pontos fortes: o aumento das interações na plataforma, em particular nos fóruns e chats; as interações geraram uma dinâmica na evolução dos estudantes no que diz respeito ao desenvolvimento das suas competências no seu sucesso académico; a avaliação, por explicitar os critérios e indicadores, tornou-se mais transparente e orientadora do trabalho dos estudantes e do professor. Porém foram identificados alguns pontos menos fortes, nomeadamente: a sobrecarga de trabalho do professor e a não adesão total dos estudantes à metodologia proposta. Dada a natureza exploratória do estudo sugere-se a sua continuidade, através do envolvimento de outros professores e estudantes.

Organic-walled dinoflagellate cysts in recent sediments from the Guadiana river estuary, South-East Portugal

Dissertação de Mestrado, Biologia Marinha, Faculdade de Ciências do Mar e do Ambiente, Universidade do Algarve, 2007

Molecular genetic analysis of a cattle population to reconstitute the extinct Algarvia breed

Background: Decisions to initiate conservation programmes need to account for extant variability, diversity loss and cultural and economic aspects. Molecular markers were used to investigate if putative Algarvia animals could be identified for use as progenitors in a breeding programme to recover this nearly extinct breed. Methods: 46 individuals phenotypically representative of Algarvia cattle were genotyped for 27 microsatellite loci and compared with 11 Portuguese autochthonous and three imported breeds. Genetic distances and factorial correspondence analyses (FCA) were performed to investigate the relationship among Algarvia and related breeds. Assignment tests were done to identify representative individuals of the breed. Y chromosome and mtDNA analyses were used to further characterize Algarvia animals. Gene- and allelic-based conservation analyses were used to determine breed contributions to overall genetic diversity. Results: Genetic distance and FCA results confirmed the close relationship between Algarvia and southern Portuguese breeds. Assignment tests without breed information classified 17 Algarvia animals in this cluster with a high probability (q > 0.95). With breed information, 30 cows and three bulls were identified (q > 0.95) that could be used to reconstitute the Algarvia breed. Molecular and morphological results were concordant. These animals showed intermediate levels of genetic diversity (MNA = 6.0 ± 1.6, Rt = 5.7 ± 1.4, Ho = 0.63 ± 0.19 and He = 0.69 ± 0.10) relative to other Portuguese breeds. Evidence of inbreeding was also detected (Fis = 0.083, P < 0.001). The four Algarvia bulls had Y-haplotypes H6Y2 and H11Y2, common in Portuguese cattle. The mtDNA composition showed prevalence of T3 matrilines and presence of the African-derived T1a haplogroup. This analysis confirmed the genetic proximity of Algarvia and Garvonesa breeds (Fst = 0.028, P > 0.05). Algarvia cattle provide an intermediate contribution (CB = 6.18, CW = -0.06 and D1 = 0.50) to the overall gene diversity of Portuguese cattle. Algarvia and seven other autochthonous breeds made no contribution to the overall allelic diversity. Conclusions: Molecular analyses complemented previous morphological findings to identify 33 animals that can be considered remnants of the Algarvia breed. Results of genetic diversity and conservation analyses provide objective information to establish a management program to reconstitute the Algarvia breed.