O desenvolvimento da competência ortográfica para melhoria da produção textual

Relatório de Estágio apresentado à Escola Superior de Educação de Lisboa para obtenção de grau de mestre em Ensino do 1.º e do 2.º Ciclo do Ensino Básico

Simple laccase-based biosensor for formetanate hydrochloride quantification in fruits

This work describes the development of an electrochemical enzymatic biosensor for quantification of the pesticide formetanate hydrochloride (FMT). It is based on a gold electrode modified with electrodeposited gold nanoparticles and laccase. The principle behind its development relies on FMT's capacity to inhibit the laccase catalytic reaction that occurs in the presence of phenolic substrates. The optimum values for the relevant experimental variables such as gold nanoparticles electrochemical deposition (at − 0.2 V for 100 s), laccase immobilization (via glutaraldehyde cross-linking), laccase concentration (12.4 mg/mL), substrate selection and concentration (5.83×10−5 M of aminophenol), pH (5.0), buffer (Britton–Robinson), and square-wave voltammetric parameters were determined. The developed biosensor was successfully applied to FMT determination in mango and grapes. The attained limit of detection was 9.5×10−8 ± 9.5×10−10 M (0.02 ± 2.6×10−4 mg/kg on a fresh fruit weight basis). Recoveries for the five tested spiking levels ranged from 95.5 ± 2.9 (grapes) to 108.6 ± 2.5% (mango). The results indicated that the proposed device presents suitable characteristics in terms of sensitivity (20.58 ± 0.49 A/μM), linearity (9.43×10−7 to 1.13×10−5 M), accuracy, repeatability (RSD of 1.4%), reproducibility (RSD of 1.8%) and stability (19 days) for testing of compliance with established maximum residue limits of FMT in fruits and vegetables.

Fresh-cut aromatic herbs: Nutritional quality stability during shelf-life

Fresh-cut vegetables are a successful convenient healthy food. Nowadays, the presence of new varieties of minimally processed vegetables in the market is common in response to the consumers demand for new flavours and high quality products. Within the most recent fresh-cut products are the aromatic herbs. In this work, the objective was to evaluate the nutritional quality and stability of four fresh-cut aromatic herbs. Several physicochemical quality characteristics (colour, pH, total soluble solids, and total titratable acidity) were monitored in fresh-cut chives, coriander, spearmint and parsley leaves, stored under refrigeration (3 ± 1 ºC) during 10 days. Their nutritional composition was determined, including mineral composition (phosphorous, potassium, sodium, calcium, magnesium, iron, zinc, manganese and copper) and fat- and water-soluble vitamin contents. Total soluble phenolics, flavonoids and the antioxidant capacity were determined by spectrophotometric methods. The aromatic herbs kept their fresh appearance during the storage, maintaining their colour throughout shelf-life. Their macronutrient composition and mineral content were stable during storage. Coriander had the highest mineral and fatsoluble vitamin content, while spearmint showed the best scores in the phenolic, flavonoid and antioxidant capacity assays. Vitamins and antioxidant capacity showed some variation during storage, with a differential behaviour of each compound according to the sample.

Língua adicional: contextos e continua

O papel de uma língua adicional, seja ela uma língua estrangeira, língua segunda, ou uma variedade não nativa, é fundamental atualmente não só no domínio profissional mas também em termos de investigação científica, mais precisamente sobre o contacto de línguas. Até ao início da segunda metade do século passado, o contacto linguístico que poderá ter estado na origem das línguas crioulas gozou de especial atenção entre os linguistas. Nas últimas décadas a sua atenção virou-se para a língua segunda e as variedades não nativas. Neste artigo, apresentamos,do ponto de vista teórico, os elos de ligação, contextos e continua, entre os quatro conceitos acima referidos, em que o primeiro, língua adicional, é apresentado como hiperónimo dos outros três, língua estrangeira, língua segunda e variedade não nativa.

Os topónimos algarvios na Paleontologia portuguesa

Since the XIX century, Portuguese and foreign geologists have defined 47 new invertebrate taxa (foraminifera, ostracods, coelenterates, brachiopods, gastropods, ammonoids, echinoids), 2 new fossil plant taxa (charophyte and pteridophyte) and 1 ichnofossil, using toponymy from the Algarve; these taxa refer to 1 genera, 47 species and 2 varieties. Besides the Algarve toponym, the most used as specific name, twenty others have been used, mostly from western Algarve; these toponyms are associated to: – Miocene units, particularly from Ribeira de Cacela and Ferragudo; – Cretaceous units between Zavial and Marim; – Upper Jurassic units from Sagres, Carrapateira and Loulé and Middle Jurassic units from Sagres and Guilhim; – Triassic units from Vila do Bispo to Tavira; – Carboniferous units, particularly from the Aljezur-Bordeira-Carrapateira region. The earliest of these designations were attributed to seven gastropods from the Upper Miocene of Cacela (COSTA, 1866-1867). The majority of the named species are typical of the Algarve, but some have been collected, as well, in the Lusitanian Basin. Although extensively cited in the geological literature, some of these taxa either do not fulfill the ICZN rules, or fall into synonymy with previously established taxa, or should be formally considered as non valid names (nomem nudum and nomen oblitum). Only widespread bibliographical review, associated with the palaeontological revision of some of these groups and the correct interpretation of the ICZN articles, will allow confirming, or not, the doubts that have now arisen.

Valorization of traditional portuguese apples and cherries: biochemical characterization and development of functional ingredients

Dissertation presented to obtain a Ph.D degree in Engineering and Technology Sciences, Biotechnology at the Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa

Genetics of berry colour and anthocyanin content variation in grapevine (Vitis vinifera L.subsp. vinifera)

Dissertation presented in fulfillment of the requirements for the Degree of Doctor of Philosophy in Biology (Molecular Genetics) at the Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa

O lugar da Hispanoamérica no processo de ensinoaprendizagem de Espanhol como Língua Estrangeira no Ensino Secundário Português

Tendo em conta um enfoque comunicativo experiencial (Fernández- Corbacho, 2014) e uma pedagogia crítica emancipatória (Jiménez Raya, Lamb & Vieira, 2007), enriquecida por enfoques multissensoriais (Arslan, 2009), é nossa intenção, com este projeto, contribuir para a implementação de práticas que espelhem as variedades linguísticas e culturais da Hispanoamérica (Liceras, 1995; Beave, 2000) na aula de espanhol como língua estrangeira no ensino secundário português. Neste estudo, através duma perspetiva metodológica de índole qualitativa, pretendemos, como ponto de partida, analisar: a) as representações de alunos portugueses sobre o lugar da Hispanoamérica no processo de ensino-aprendizagem de espanhol como língua estrangeira (Altmann & Vences, 2004; Pérez, 2003), através de inquéritos por questionário; e, ainda, b) as abordagens das variedades linguísticas e culturais do espanhol, que surgem nos manuais utilizados no ensino secundário português. Por outro lado, através de um estudo de caso (Benson, Chik, Gao, Huang & Wang, 2009), procurámos evidenciar uma mostra de possíveis boas práticas didático-pedagógicas e materiais, com vista a um trabalho sistemático e próativo com as variedades linguísticas e culturais do espanhol, baseado numa (hiper)pedagogia crítica e encarando a língua enquanto objeto manipulável e potenciador de cidadãos verdadeiramente conscientes do mundo. Para tal, criámos materiais físicos e digitais, que foram posteriormente implementados com alunos do 11º ano de escolaridade, no nível de iniciação de espanhol, num agrupamento de escolas da região de Aveiro. Os resultados mostram que práticas e materiais desta natureza poderão favorecer aprendizagens comunicativas experienciais, quanto à criação de futuros cidadãos críticos e ativos, fomentando o desenvolvimento das suas competências comunicativa plurilingue e pluricultural e duma consciência cultural crítica (Byram, Gribkova & Starkey, 2002) dos alunos, no contexto de ensino-aprendizagem do ensino secundário.

Characterization of Cryptococcus neoformans isolated from urban environmental sources in Goiânia, Goiás State, Brazil

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis as the most frequent clinical presentation in immunocompromised patients, mainly in people infected by HIV. This fungus is an environmental encapsulated yeast, commonly found in soil enriched with avian droppings and plant material. A total of 290 samples of pigeon and the other avian droppings, soil, ornamental trees and vegetable material associated with Eucalyptus trees were collected to study environmental sources of Cryptococcus species in Goiânia, Goiás State. The determination of varieties, serotypes and the susceptibility in vitro to fluconazole, itraconazole and amphotericin B of C. neoformans isolates were performed. C. neoformans var. grubii (serotype A) was found in 20.3% (36/177) of pigeon dropping samples and in 14.3% (5/35) of samples of Eucalyptus. None of the environmental isolates of C. neoformans showed in vitro resistance to three antifungal agents. The knowledge of major route for human cryptococcal infection (inhalation of infectious particles from saprophytic sources) and a total of 60 C. neoformans isolates obtained from AIDS patients with cryptococcal meningitis between October 2001 and April 2002 justify the study of the habitats of these yeasts as probable sources of cryptococcosis in this city.

Novel transcription factors regulating the expression of the rice gene OsDREB1B

Dissertation presented to obtain the Ph.D degree in Biology

The institutions of archaic post-modernity and their organizational and managerial consequences: The case of Portugal

The long march of modernization of the Western societies tends to be presented as following a regular sequence: societies and institutions were pre-modern, and then they were modernized, eventually becoming post-modern. Such teleology may provide an incomplete or distorted narrative of societal evolution in many parts of the world, even in the ‘post-modern heartland’ of Western Europe, with Portugal being a case in point. The concept of archaic post-modernity has been developed by a philosopher, José Gil, to show how Portuguese institutions and organizations combine elements of pre-modernity and post-modernity. The notion of an archaic post-modernity is advanced in order to provide an alternative account of the modernization process, which enriches discussion of the varieties of capitalism. Differences in historical experiences create singularities that may be considered in the analysis of culture, management and organization.

Evolution of methodology for the study of adaptability and stability in cultivated species.

The GxE interaction only became widely discussed from evolutionary studies and evaluations of the causes of behavioral changes of species cultivated in environments. In the last 60 years, several methodologies for the study of adaptability and stability of genotypes in multiple environments trials were developed in order to assist the breeder's choice regarding which genotypes are more stable and which are the most suitable for the crops in the most diverse environments. The methods that use linear regression analysis were the first to be used in a general way by breeders, followed by multivariate analysis methods and mixed models. The need to identify the genetic and environmental causes that are behind the GxE interaction led to the development of new models that include the use of covariates and which can also include both multivariate methods and mixed modeling. However, further studies are needed to identify the causes of GxE interaction as well as for the more accurate measurement of its effects on phenotypic expression of varieties in competition trials carried out in genetic breeding programs.

The Internationalization of a Portuguese small and medium enterprise: The case of Vale da Rosa

A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA – School of Business and Economics

Schottky principal G-bundles over compact Riemann surfaces

Fundação para a Ciência e a Tecnologia (FCT)- PhD grant SFRH/BD/37151/2007; projects PTDC/MAT/099275/2008; PTDC/MAT/119689/2010; PTDC/MAT/120411/2010; PTDC/MAT-GEO/0675/2012

Study of salt stress tolerance in spelt

World population is increasing at an alarming rate while food productivity is decreasing due to the effect of various abiotic stresses. Soil salinity is one of the most important abiotic stress and a limiting factor for worldwide plant production. In addition to its important effects on yield, salt stress affects numerous cellular activities, including cell wall composition, photosynthesis, protein synthesis, ions and organic solutes. Up to 20% of the irrigated arable land in arid and semiarid regions is already salt affected and is still expanding. Improving salt tolerant varieties is of major importance, and efforts should be focused on finding adaptive mechanisms which are involved in salinity tolerance. In this study, several spelt wheat (Triticum aestivum var. Spelta) genotypes and one cultivar of modern bread wheat were used to screen them for salt tolerance. Spelt is an old-European cereal crop currently attracting renewed interest as a food grain because it is said to be harder than wheat and requires less fertilizer. Spelt wheat is also becoming very attractive genetic source by plant breeders due to its wide adaptation ability to various stressful conditions such as soil salinity. In this study morphological parameters (e.g., leaf appearance; shoot elongation), dry matter production, mineral nutrients (especially Na and K), and activity of antioxidative enzymes were measured to select superior genotypes of spelt for salt tolerance. The results showed that Spelt genotype Sp41 is a salt sensitive genotype and genotypes Sp69, Sp96 and Sp912 are good candidates for salt tolerant genotypes.