965 resultados para Genes Regulatory Sequences
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
The present study aimed to cytogenetic analysis and structural and molecular level of four fish species of the genus Trichomycterus: T. diabolus, T. iheringi, T. zonatus and T. cf. mimonha collected in different river basins in Brazil. Techniques were used for classical cytogenetic (Giemsa, Silver nitrate impregnation, C-banding) and molecular with the chromosomal location of genes for 18S and 5S rDNA. All individuals examined had a diploid number 54 chromosomes and karyotype consisting of types of metacentric, submetacentric and subtelocentric. The constitutive heterochromatin identified by C-banding was observed in two small blocks in the karyotype of T. diabolus and large blocks of centromeric several pairs in chromosomal karyotypes of T. iheringi, T. zonatus and T. cf. mimonha. The Silver nitrate impregnation and hybridization with 18S rDNA probe revealed the existence of only a couple carryng nucleolar organizing regions (NORs) on the species T. diabolus, T. iheringi and T. cf mimonha, and two pairs carrying 18S rDNA in T. zonatus. The 5S rDNA was observed in interstitial position 6 of the pair in T. iheringi, the synteny with the two pair in 18S rDNA of T.diabolus in pericentromeric position of and two pairs submetacentric, one being also a case of synteny with the 18S rDNA in T. zonatus and T. cf. mimonha, this rDNA was located in the pairs 3, 18 and 25, and synteny in the 18S rDNA pair 18. Although representatives of these four species Trichomycterus present diploid number and karyotypic formula preserved, three is specific about the distribuition patterns of heterochromatin and location of rDNA sequences, indicating that chromosomal differentiation events in this group of fish are acting directly on these genomic portions
Resumo:
Pós-graduação em Microbiologia Agropecuária - FCAV
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Pós-graduação em Genética e Melhoramento Animal - FCAV
Resumo:
Pós-graduação em Microbiologia Agropecuária - FCAV
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Pós-graduação em Biologia Animal - IBILCE
Resumo:
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Interferon regulatory factor 1 (IRF1) is functionally diverse in the regulation of immune response and is considered to be an important candidate gene for studying disease susceptibility in mammals. In this paper, we characterized the whole sequence of the IRF1 gene in river buffalo (Bubalus bubalis) and compared genomic and the amino acid sequences between different species. The buffalo IRF1 gene was 7099 bp long and organized into 10 exons and nine introns. Its molecular structure showed exactly the same number of exons (10) and introns (nine) in bovids, mice, horses, humans, and chickens. However, rats did not have exon 5, but had the largest exon 4, which suggests that exon 5 was incorporated into exon 4. The coding and the amino acid sequences of the gene showed that identity varied from 73 to 99% at the coding sequence level and from 61 to 100% at the amino acid level when compared with other mammals and chickens. Comparative analysis of the gene sequence between two different buffalo breeds, Murrah and Mediterranean, revealed six potential SNPs that are primarily located in the 5' and 3'UTRs.
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Resumo:
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)