Expansão ex vivo de células uroteliais em scaffolds 3D

Malformations and possible damages to the urogenital system can be originated in the embryonic period. Moreover, fire guns, knives and accidents, where there is the disruption of the urethra, also cause these lesions. The objective was to analyze the contribution of tissue engineering in the construction of neo-urethra, developed by bioengineering. We performed an urothelial ex vivo expansion of cells in 3D scaffolds (platelet gel matrix and acellular porcine aorta) to assess the contribution of this technique in the construction of a neo-urethra. Mechanical dissociation was made of the inner wall of 10 North Folk rabbit’s bladder, weighing 2.5 to 3.0 kg. After dissociation the cell content was centrifuged and obtained a pellet of urothelial cells. The pellet was ressuspended in culture medium DMEM F12 and cells were maintained in culture for 15 days. Immunohistochemical analysis characterized the urothelial culture. The cells were then implanted in the scaffold - platelet gel. In a second experiment using aortic porcine acellular matrix were implanted urothelial cells alone and urothelial cells on platelet gel, on the inner wall of the scaffold - aorta, with space for setting bordered by a urethral probe. The complex probe - cells - aorta and probe - cells in platelet gel - aorta, were sealed with suture material and culture were maintained in a humidified 37ºC incubator with 5% CO2 in air for 12 days to subsequent histological analysis of urothelium cell adhesion to the scaffolds. By observation under an optical microscope, we could see the growth of cells in the scaffold platelet gel, from a monolayer in to a three-dimensional structure. In the acellular porcine aortic matrix containing the platelet gel, we could observe a few quantity of urothelial cells adhered. However with the acellular porcine aortic matrix in which was implanted only the urothelial cells, we have obtained adhesion to the wall

Classificação de workflows baseados em web services

Over the last decades changes have occurred in communication within and between enterprises, made easier by technologies suchas E-commerce, Internet, ERP systems and remote meetings and there was a rapid progress in network technology, which has changed the way business is done. A standardized way to offer services over the internet is using web services. Web services are a kind of remote procedure call and are generally used to integrate systems, independent of language, both client and server. It is common to use several web services run in sequence to perform a business process. To this type of process, gives the name of workflow. Thus, Web services are the primary components of workflows. A tool that provides a way of visualizing the behavior of a workflow can assist the administrator and is required. The present work presents the development of a tool that allows the administrator to classify visually services components and evaluate their importance in the final performance of a workflow. As proof of concept we used several virtual servers and computers where each computer has received a set of web services. A proxy was added between each call of workflows collecting relevant information and storing them in a database for later analysis. The analysis was based on Quality of Service parameters

Braquiterapia com sementes de iodo-125: manufatura e tratamento

According to the National Institute of Cancer – INCA, 466.730 new cancer cases will occur in Brazil in 2009. Prostate and Lung cancer in man are the most incident types (in exception of the non-melanoma cancer). The brachytherapy with 125-iodine sources is an important method of prostate cancer treatment. The implant with iodine-125 seeds uses aproximately 100 seeds that are imported impossibilitating the treatment in large scale. For this reason, a multidisciplinary group was created at the Energetic and Nuclear Research Institute – Radiation Technology Center (IPEN –CTR / SP) to develop a national 125-iodine source and established a facility for local production. The seeds manufacture in Brazil will diminish the cost of treatment and prostate brachytherapy will be offered to more patients. This work aim is to observe and discuss the methods used in seeds manufacture there are being developed in Brazil and to present an prostate cancer case folloied in A.C. Camargo Hospital. The 125-iodine is adsorbed in an silver wire, then deposited at titanium coat. The weld is made by an process of plasm sealing. The seeds goes through several test to guarantee that there is no leakage. The result is an high quality and cheaper product. The implant tecnique is an fast and save procedure. The medical physicst preplan the case to stipulate the quantity of seeds there will be used. At the dat of the implant the medical physicst replan the procedure due to changes of volume in the organ. That assure the correct dose distribution in the target. Besides, the 125-iodine low energy is absorbed at the volume of interrest saving sourronding healthy tissues such as the rectum and the urethra

Evolução da tecnologia móvel até 2013

In this paper expounds on the major phases or generations of the evolutionary process of wireless mobile communication, emphasizing their characteristics and changes over time. The main benefit of this process is the rate of data transfer remembering capacity spectral and methods of harnessing and improving its efficiency. The current network today is 4G and the technologies developed over the following requirements of the ITU are: Long Term Evolution Advanced (LTE-Advanced) and WirelessMAN-Advanced as part of the WiMAX IEEE 802.16m also described in this graduate work

Avaliação de projetos logísticos de RFID aplicados na indústria aeronáutica brasileira

Pós-graduação em Engenharia Mecânica - FEG

A caridade é, em tudo, a regra de proceder: análise do discurso espírita kardecista

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Memória e Memórias: entre o eu da ficção e a ficcionalização do eu

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

João Miramar e as dobras do texto

This study about Memórias Sentimentais de João Miramar, by Oswald de Andrade, aims to explore the voices that organize the space of language in the narrative. Such space has an operative vision called polyphony, by which the signs organize themselves out of a hierarchical scale, although the chronological axis of the life of João Miramar is the guiding principle of the episodes.

Telemetria automotiva usando módulo de transmissão Wireless Zigbee

This paper presents a design and construct the system of data acquisition with wireless transmission of some important parameters like motor temperature, motor rotation and velocity of Unesp Racing´s formula car prototype from Universidade Estadual Paulista, Campus Guaratinguetá. This system development presents a contribution for the electrical technical features adding important points in the competition due car´s electric, in addition, enables preventive maintenance and fine adjustment of the car with the data obtained during training and racing

Saúde e qualidade de vida de adolescentes de um assentamento rural no Pontal do Paranapanema-SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeito da atividade física na sensibilidade à insulina e no sinal insulínico em ratos com doença periodontal

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transmissão indutiva de energia eletromagnética sem fios, para aplicações em postos de abastecimento de veículos elétricos puros: modelação para o elemento eletromagnético

Pós-graduação em Engenharia Elétrica - FEIS

Youtube: práticas discursivas e identitárias no ciberespaço

Inegavelmente, as mídias tradicionais como a televisão, o rádio e a mídia impressa detêm grande influência na sociedade contemporânea, tanto para o delineamento do pensamento coletivo, quanto para a sugestão à formação cultural por meio de enunciados produzidos por sujeitos que ocupam determinadas formações sócio- ideológicas. Entretanto, atualmente, um objeto que tem ganhado espaço privilegiado, diferente das mídias tradicionais, são as mídias digitais. Fluidos no grande oceano do ciberespaço que se constitui como um aglutinador de mídias, onde se mesclam mídias tradicionais e novas mídias, centros recepto-difusores encontram-se dispersos em toda parte e, efetivamente, em parte alguma, tornando-se, assim, um espaço onde a cultura participativa e (d)a interatividade ganham traços. Dessa maneira, o presente trabalho, embasado principalmente pelo aporte teórico da Análise do Discurso de linha francesa, tendo como fio condutor as reflexões de Michel Foucault, procura discutir questões de identidades produzidas e veiculadas nos meios digitais. Toma-se como corpus de análise vídeos caseiros de pessoas “anônimas” que transmitem-se pelo canal do YouTube, onde mostram suas habilidades vocais, artísticas e até mesmo conhecimentos de técnicas áudio-visuais para a produção de efeitos de sentido. Estes sujeitos fazem uso de técnicas de confissão para a construção e afirmação de uma identidade de “artista” e, por essas práticas, produzem novas subjetividades. Em torno destes vídeos, ainda, são originadas comunidades inteligentes de pertencimento pontuadas no próprio YouTube e em outras plataformas, como no Twitter, Facebook, My Space, etc. onde são mobilizados e compartilhados os savoir-faire desses sujeitos e virtualizadas e atualizadas as identidades à luz de uma cultura da convergência.

A formulação de uma corrente urbanística: uma (re)visita às teses de Anhaia Mello para São Paulo

The present article aims to elaborate an objective analytical panel, initially from the 1920- 1930 when Luiz Ignácio Romeiro de Anhaia Mello, cathedratic in the urban question, started to quote a series of authors and American plans as references to their reflections, mainly when the point was the verticalization or not at São Paulo City. From a broad bibliographic research with the systematization of the register from the debate between Anhaia Mello, this article intends to point out how the transposition of the urbanistic conceptions to the academic environment happened and, at the same time, the legal normatization of the São Paulo City that resulted in the creation of a draft of the regulations for use and occupation of the soil. The systematizations and analysis were based in a broad reading of the bibliography and documental sources that refer to the proposed theme. The books of the library of FAU-USP, specifically the one from SAGMACS – Sociedade de Análises Gráficas e Mecanográficas Aplicadas aos Complexos Sociais ( Society of Graphical Mechanical-Graphic Analysis Applied to Social Complexes) -, aiming to select papers and the collection of Anhaia Mello´s family, so that a reconstruction of the intellectual and professional journey of the Urbanism Professor. The systematization of the collected data and posterior comparison with the bibliographical study was fundamental for the reconstruction of the reflection of Anhaia Mello about the ways of the urban streets and roads at Sao Paulo City should be lead to on the middle of the XX century having as a guideline the debate between Anhaia Mello – very common on the 1950s for less important countries when dealing with the organization of the size of industrial metropolis. It was possible to identify the crystallization of one urbanistic proposal for the city: the from Anhaia Mello, that bet on the reversion of the metropolitan cycle by stopping the urban growth by the application of the theoretical conception of the garden city.

Reabilitação estético-funcional sobre implantes agulhados e endósseos

This report shows the resolution of a case study whose aim was promote the aesthetic and functional rehabilitation of upper left hemi-arch over endosseous and needle implant prostheses. To improve the position of the needle implants, we performed the bucco-palatal bending of needles splinting them with composite resin; we removed the screwed implant prosthesis in the regions of the premolars (24 and 25) and molar (26). The mini-abutments of the last two implants were replaced, and in the first premolar and canine, we used cemented abutments due to implant angulation having planned soldering because of implant size and contact with one needle implant that could interfere with intimate contact bone/implant damaging biomechanics. Pick-up impressions were performed with an open tray, using a retractor cord in the needle implants and respective transfer copings. Therefore, models were related on semi-adjustable articulator after a face-bow recording and interocclusal indexes. After working cast fabrication and try-in of infra-structures, these were adjusted and related into the articulator again. Then, after ceramic build-up, adjustments, finishing, and torque, chemically-activated resin cement was applied on units 21 to 24. At the end of the case, we concluded that a good aesthetic and functional rehabilitation depends on thorough knowledge of techniques for each clinical situation.