wrinkled1: A Novel, Low-Seed-Oil Mutant of Arabidopsis with a Deficiency in the Seed-Specific Regulation of Carbohydrate Metabolism1

During oil deposition in developing seeds of Arabidopsis, photosynthate is imported in the form of carbohydrates into the embryo and converted to triacylglycerols. To identify genes essential for this process and to investigate the molecular basis for the developmental regulation of oil accumulation, mutants producing wrinkled, incompletely filled seeds were isolated. A novel mutant locus, wrinkled1 (wri1), which maps to the bottom of chromosome 3 and causes an 80% reduction in seed oil content, was identified. Wild-type and homozygous wri1 mutant plantlets or mature plants were indistinguishable. However, developing homozygous wri1 seeds were impaired in the incorporation of sucrose and glucose into triacylglycerols, but incorporated pyruvate and acetate at an increased rate. Because the activities of several glycolytic enzymes, in particular hexokinase and pyrophosphate-dependent phosphofructokinase, are reduced in developing homozygous wri1 seeds, it is suggested that WRI1 is involved in the developmental regulation of carbohydrate metabolism during seed filling.

Bioremediation of oil in Prince William Sound, Alaska.

Mode of access: Internet.

Oil crops yearbook /

Mode of access: Internet.

Draft proposed program : 5-year outer continental shelf oil and gas leasing program for mid-1986 through mid-1991.

"March 1985."

Oil pollution research and technology plan /

"April 24, 1992."

Organizational, direct support and general support maintenance repair parts and special tools lists : heater, hot oil, 2,100,000 BTU/HR output, oil fired, electric driven, semitrailer mounted Army model 200S NSN 3895-00-066-6016, Hopkins model 200S and melter, asphalt, 900 GAL/HR output min, 12 drum, skid mounted NSN 3895-00-066-6017, Hopkins model 18BAM and NSN 3895-00-839-55986, Tar Heel Engineering and Manufacturing Co. model TH-12-750AM.

Includes index.

Organizational, direct support, and general support maintenance repair parts and special tools list : hammer, pile-driver, self-powered; diesel engine driven, w/fuel oil tank and lubricating oil tank, NSN 3895-00-854-4150, link-belt speeder model 440.

"This publication supersedes TM 5-3895-333-25P dated 18 February 1969 and all changes"--P. i.

Organizational, direct support and general support maintenance repair parts and special tools lists (including depot maintenance repair parts and special tools lists) : heater, hot oil, trailer mounted, electric motor driven, 2,100,000 BTU/HR output NSN 3895-00-838-9180, Hopkins model 200STM.

Includes index.

Organizational direct support, and general support maintenance repair parts and special tools lists : heater, bituminous, trailer mounted, 3 tank car heating capacity, oil-fired steam boiler, gasoline engine, driven feed water pump, NSN 3895-00-062-7912, Cleaver Brooks model PSM 50M.

Includes index.

Operator, organizational, direct support, general support, and depot maintenance manual : hammer, pile-driver, self-powered, w/fuel oil tank and lubricating oil tank (Link-Belt Speeder mdl 440) FSN 3895-854-4150.

"December 1968."

Operator and organizational maintenance manual : tank, steel, vertical, bolted, knockdown, sealed openings, standard bottom and roof, gasoline, oil, or water (type I-pol and potable water, type II-pol and non-potable water), 100 barrel capacity ... 10,000 barrel capacity ....

"May, 1974."

Proposed final outer continental shelf oil and gas leasing program, 1997 to 2002 : decision document.

"August 1996."

Proposed 5-year outer continental shelf oil and gas leasing program mid-1987 to mid-1992 : final environmental impact statement /

Mode of access: Internet.

Studies on enhanced surface disinfection and skin antisepsis using chlorhexidine and eucalyptus oil

Healthcare associated infections may arise from many sources, including patient?s own skin flora and the clinical environment, and inflict a significant burden within the health service. Adequate and effective skin antisepsis and surface disinfection are therefore essential factors in infection control. Current EPIC guidelines recommend 2 % chlorhexidine (CHG) in 70 % isopropyl alcohol (IPA) for skin antisepsis however poor penetration has been reported. Eucalyptus oil (EO) is a known permeation enhancer, producing synergistic antimicrobial activity when combined with CHG. In this current study, the antimicrobial efficacy of EO and its main constituent 1,8-cineole were assessed against a panel of clinically relevant microorganisms, alone and in combination with CHG. The superior antimicrobial efficacy of EO compared with 1,8-cineole, and synergistic effects with CHG against planktonic and biofilm cultures, confirmed its suitability for use in subsequent studies within this thesis. Impregnation of EO, CHG and IPA onto prototype hard surface disinfectant wipes demonstrated significantly improved efficacy compared with CHG/IPA wipes, with clear reductions in the time required to eliminate biofilms. Optimisation of the EO/CHG/IPA formulation resulted in the development of Euclean® wipes, with simulated-use and time kill studies confirming their ability to remove microbial surface contamination, prevent cross contamination and eliminate biofilms within 10 minutes. The employment of isothermal calorimetry provided additional information on the type and rate of antimicrobial activity possessed by Euclean® wipes. A clinical audit of the Euclean® wipes at Birmingham Children?s Hospital, Birmingham, U.K. revealed divided staff opinion, with the highest cited advantage and disadvantage concerning the odour. Finally, skin penetration and cell toxicity studies of EO/CHG biopatches and Euclean® solution developed during this study, revealed no permeation into human skin following biopatch application, and no significant toxicity. These current studies enhance the knowledge regarding EO and its potential applications.

Antimicrobial efficacy of chlorhexidine digluconate alone and in combination with eucalyptus oil, tea tree oil and thymol against planktonic and biofilm cultures of Staphylococcus epidermidis

Objectives Effective skin antisepsis and disinfection of medical devices are key factors in preventing many healthcare-acquired infections associated with skin microorganisms, particularly Staphylococcus epidermidis. The aim of this study was to investigate the antimicrobial efficacy of chlorhexidine digluconate (CHG), a widely used antiseptic in clinical practice, alone and in combination with tea tree oil (TTO), eucalyptus oil (EO) and thymol against planktonic and biofilm cultures of S. epidermidis. Methods Antimicrobial susceptibility assays against S. epidermidis in a suspension and in a biofilm mode of growth were performed with broth microdilution and ATP bioluminescence methods, respectively. Synergy of antimicrobial agents was evaluated with the chequerboard method. Results CHG exhibited antimicrobial activity against S. epidermidis in both suspension and biofilm (MIC 2–8 mg/L). Of the essential oils thymol exhibited the greatest antimicrobial efficacy (0.5–4 g/L) against S. epidermidis in suspension and biofilm followed by TTO (2–16 g/L) and EO (4–64 g/L). MICs of CHG and EO were reduced against S. epidermidis biofilm when in combination (MIC of 8 reduced to 0.25–1 mg/L and MIC of 32–64 reduced to 4 g/L for CHG and EO, respectively). Furthermore, the combination of EO with CHG demonstrated synergistic activity against S. epidermidis biofilm with a fractional inhibitory concentration index of <0.5. Conclusions The results from this study suggest that there may be a role for essential oils, in particular EO, for improved skin antisepsis when combined with CHG.