Sequences of immunoglobulin chains : tabulation and analysis of amino acid sequences of precursors, V-regions, C-regions, J-chain and -microglobulins /

1976 ed. issued under title: Variable regions of immunoglobulin chains.

Ascorbic acid; vitamin C in wound healing; Annotated bibliography.

Mode of access: Internet.

Physiologic activity and clinical use of Ascorbic Acid (Vitamin C).

Bibliography: leaves 47-59.

The new American orchardist; or, An account of the most valuable varieties of fruit, adapted to cultivation in the climate of the United States, from the latitude of 25 [degrees] to 54 [degrees], with their uses, modes of culture, and management; remedies for the maladies to which they are subject, from noxious insects, and other causes, &c. Also, a brief description of the most ornamental forest trees, shrubs, flowers, &c.

Mode of access: Internet.

Grosse sinfonie für 2 violinen, viola, violincell, bass, 1 flöte, 2 clarinetten, 2 fagotten, 2 hörner in Es, 2 oboen, 2 hörner in C, posaune, trompeten und pauken ... Op. 25. Ausgabe nach dem original manuscript mit vom autor hinzugegügten 2 oboen, 2 hörnern in C und posaune.

"Partitur."

The experienced English housekeeper : for the use and ease of ladies, housekeepers, cooks, &c., written purely from practice : dedicated to the Hon. Lady Elizabeth Warburton, whom the author lately served as housekeeper : consisting of several hundred original receipts, most of which never appeared in print /

Includes recipes for wine, mead, and other liquors; illustrations of kitchen stoves using coal; illustrations of layout of dishes for multi-course meal. Sample recipes: To bake herrings, To make cream pancakes, To make a blanc-mange of isinglass, To make cowslip wine.

A treatise on the French verbs, regular, irregular, defective, &c. with notes and illustrations, selected from lthe most approved grammarians.

Mode of access: Internet.

Operator's manual for crane, truck mounted, hydraulic, 25 ton (CCE), Grove model TM S-300-5, contract no. DSA 700-77-C-8511, NSN 3810-01-054-9779.

"1 May 1980."

Report of the International Council of Women : assembled by the National Woman Suffrage Association, Washington, D.C., U.S. of America, March 25 to April 1, 1888 /

Includes index.

Superfund implementation : hearings before the Subcommittee on Superfund and Environmental Oversight of the Committee on Environment and Public Works, United States Senate, One hundredth Congress, first session, April 14, 1987, Newark, N.J.; April 29, June 25, and July 23, 1987, Washington, D.C.

Item 1045-A, 1045-B (microfiche)

Evolution of the regular zone of histone H1 in Fabaceae plants

An analysis of the historic H1 subtype, H1-1, in eight legumes belonging to four genera of the tribe Vicieae (Pisum, Lathyrus, Lens, and Vicia), revealed an extended region consisting of the tandemly repeated AKPAAK motifs. We named this region the Regular zone (RZ). The AKPAAK motifs are organized into two blocks separated by a short (two or six amino acids) intervening sequence (IS). The distal block contains six AKPAAK motifs, while the number of repeats in the proximal block varies from six in V. faba to seven in the other species. In V. hirsuta, the first two repeated units of the proximal block are octapeptides AKAKPAAK. The apparent rate of synonymous substitutions in the blocks of RZ is much higher than in the rest of the gene. This can be explained by repeat shuffling within each block. In the C-domain of the orthologous H1 subtype froth Medicago truncatula (tribe Trifolieae), a region corresponding to the RZ of Vicieae species was found. It also consists of two blocks of AKPAAK motifs (four and three repeats in the proximal and distal blocks, respectively). These blocks are separated by a 20-amino acid IS. The first 20 amino acids of the Medicago RZ are not part of AKPAAK repeats. We hypothesise that the RZ has most probably evolved as a result of an expansion of AKPAAK repeats from two separate sites in the C-domain. This process started tens of millions of years ago and was most likely directed by positive selection.

Human tartrate-resistant acid phosphatase becomes an effective ATPase upon proteolytic activation

Proteolytic, cleavage in an exposed loop of human tartrate-resistant acid phosphatase (TRAcP) with trypsin leads to a significant increase in activity. At each pH value between 3.25 and 8.0 the cleaved enzyme is more active. Substrate specificity is also influenced by proteolysis. Only the cleaved form is able to hydrolyze unactivated substrates efficiently, and at pH > 6 cleaved TRAcP acquires a marked preference for ATP. The cleaved enzyme also has altered sensitivity to inhibitors. Interestingly, the magnitude and mode of inhibition by fluoride depends not only on the proteolytic state but also pH. The combined kinetic data imply a role of the loop residue D158 in catalysis in the cleaved enzyme. Notably, at low pH this residue may act as a proton donor for the leaving group. In this respect the mechanism of cleaved TRAcP resembles that of sweet potato purple acid phosphatase. (c) 2005 Elsevier Inc. Ail rights reserved.

Stable production of hyaluronic acid in Streptococcus zooppidemicus chemostats operated at high dilution rate

Hyaluronic acid is routinely produced through fermentation of both Group A and C streptococci. Despite significant production costs associated with short fermentations and removal of contaminating proteins released during entry into stationary phase, hyaluronic acid is typically produced in batch rather than continuous culture. The main reason is that hyaluronic acid synthesis has been found to be unstable in continuous culture except at very low dilution rates. Here, we investigated the mechanisms underlying this instability and developed a stable, high dilution rate (0.4 h(-1)) chemostat process for both chemically defined and complex media operating for more than 150 h of production. In chemically defined medium, the product yield was 25% higher in chemostat cultures than in conventional batch culture when arginine or glucose was the limiting substrate. In contrast, glutamine limitation resulted in higher ATP requirements and a yield similar to that observed in batch culture. In complex, glucose-limited medium, ATP requirements were greatly reduced but biomass synthesis was favored over hyaluronic acid and no improvement in hyaluronic acid yield was observed. The successful establishment of continuous culture at high dilution rate enables both commercial production at reduced cost and a more rational characterization and optimization of hyaluronic acid production in streptococci. (c) 2005 Wiley Periodicals, Inc.

Aryliminopropadienone-C-amidoketenimine-amidinoketene-2-aminoquinolone cascades and the ynamine-isocyanate reaction

Imidoylketenes 11 and oxoketenimines 12 are generated by flash vacuum thermolysis of Meldrum's acid derivatives 9, pyrrolediones 17 and 18, and triazole 19 and are observed by IR spectroscopy. Ketenimine-3-carboxylic acid esters 12a are isolable at room temperature. Ketenes 11 and ketenimines 12 undergo rapid interconversion in the gas phase, and the ketenes cyclize to 4-quinolones 13. When using an amine leaving group in Meldrum's acid derivatives 9c, the major reaction products are aryliminopropadienones, ArN=C=C=C=O (15). The latter react with 1 equiv of nucleophile to produce ketenimines 12 and with 2 equiv to afford maIonic acid imide derivatives 16. N-Arylketenimine-C-carboxamides 12c cyclize to quinolones 13c via the transient amidinoketenes 11c at temperatures of 25-40 degrees C. This implies rapid interconversion of ketenes and ketenimines by a 1,3-shift of the dimethylamino group, even at room temperature. This interconversion explains previously poorly understood outcomes of the ynamine-isocyanate reaction. The solvent dependence of the tautomerism of 4-quinolones/4-quinolinols is discussed. Rotational barriers of NMe2 groups in amidoketenimines 12c and malonioc amides and amidines 16 (24) are reported.