Lottery Action, Vol. 16, No. 01

Iowa Lottery newsletter for lottery retailers.

Effect of conditioned media, nutritive elements, and mitotic synchronization on the accuracy of the cytogenetic analysis in acute nonlymphocytic leukemia patients presenting with inv(16)/t(16;16) or t(15;17).

To improve the yield of the cytogenetic analysis in patients with acute nonlymphocytic leukemia (ANLL), six culture conditions for bone marrow or peripheral blood cells were tested in parallel. Two conditioned media (CM), phytohemagglutinin leukocyte PHA-LCM and 5637 CM, nutritive elements (NE), and methotrexate (MTX) cell synchronization were investigated in 14 patients presenting with either inv(16)/ t(16;16) (group 1, n = 9 patients) or t(15;17) (group 2, n = 5). The criteria used to identify the most favorable culture conditions were the mitotic index (MI), the morphological index (MorI), and the percentage of abnormal metaphases. In the presence of PHA-LCM and 5637 CM, the MI were significantly increased in group 2, whereas in the MTX conditions, MI remained very low in both groups. The values of the MorI did not reveal any significant changes in chromosome resolution between the conditions in either group. The addition of NE did not have a positive effect in quantity or quality of metaphases. Because of the variability of the response of leukemic cells to different stimulations in vitro, several culture conditions in parallel are required to ensure a satisfactory yield of the chromosome analysis in ANLL.

[Lettre autographe signée de Giuseppe Verdi à Escudier (sans lieu), (16 août 1867)]

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[Lettre autographe signée de Giuseppe Verdi adressée à Escudier, Naples, 16 novembre 1872]

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EPI Update, April 16, 2010

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health.

Iowa Workforce Monthly, May 2010, Issue 16

Highlights: * On Wednesday, April 7th, the Marshalltown Workforce Center and the local New Iowan Center participated in the regional Central Iowa Job Fair.....pg. 2 * The Burlington Workforce Center had a successful year offering Iowans free income tax assistance through the Volunteer Income Tax Assistance (VITA) program.............................................pg. 2 * The New Iowan Center and the Muscatine Workforce Center will be holding a Spring Career Fair on Thursday, May 20th.......pg. 2 * Iowans gathered Wednesday, April 28, to pay tribute and to reflect upon the lives of the Iowa workers who lost their lives in 2009 while at work............................pg. 3

Infonet, January 16, 2009, Issue

A publication of the Governorʼs DD Council & ID Action. It was first created nearly two decades ago as a print-only publication, sent to a few hundred people. Today we offer our nearly 3,000 direct subscribers and a variety of services.

Motor Vehicle Crash Fatalities for Week Ending, July 16, 2010

Motor Vehicle Crash Fatalities

EPI Update, July 16, 2010

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health.

Secure and Prepared Newsletter, August 26, 2010, Vol. 6, no. 16

A bi-weekly newsletter for those involved in the fields of homeland security and/or emergency management

Mucosal but not parenteral immunization with purified human papillomavirus type 16 virus-like particles induces neutralizing titers of antibodies throughout the estrous cycle of mice.

We have recently shown that nasal immunization of anesthetized mice with human papillomavirus type 16 (HPV16) virus-like particles (VLPs) is highly effective at inducing both neutralizing immunoglobulin A (IgA) and IgG in genital secretions, while parenteral immunization induced only neutralizing IgG. Our data also demonstrated that both isotypes are similarly neutralizing according to an in vitro pseudotyped neutralization assay. However, it is known that various amounts of IgA and IgG are produced in genital secretions along the estrous cycle. Therefore, we have investigated how this variation influences the amount of HPV16 neutralizing antibodies induced after immunization with VLPs. We have compared parenteral and nasal protocols of vaccination with daily samplings of genital secretions of mice. Enzyme-linked immunosorbent assay analysis showed that total IgA and IgG inversely varied along the estrous cycle, with the largest amounts of IgA in proestrus-estrus and the largest amount of IgG in diestrus. This resulted in HPV16 neutralizing titers of IgG only being achieved during diestrus upon parenteral immunization. In contrast, nasal vaccination induced neutralizing titers of IgA plus IgG throughout the estrous cycle, as confirmed by in vitro pseudotyped neutralization assays. Our data suggest that mucosal immunization might be more efficient than parenteral immunization at inducing continuous protection of the female genital tract.