Stress responses of the fish Nile tilapia subjected to electroshock and social stressors

Plasma cortisol and glucose levels were measured in 36 adult Nile tilapia males, Oreochromis niloticus (standard length, mean ± SD, 14.38 ± 1.31 cm), subjected to electroshock and social stressors. Pre-stressor levels were determined 5 days after the adjustment of the fish to the experimental aquaria (1 fish/aquarium). Five days later, the effects of stressors on both cortisol and glucose levels were assessed. The following stressors were imposed for 60 min: pairing with a larger resident animal (social stressor), or a gentle electroshock (AC, 20 V, 15 mA, 100 Hz for 1 min every 4 min). Each stressor was tested in two independent groups, one in which stress was quantified immediately after the end of the 60-min stressor imposition (T60) and the other in which stress was quantified 30 min later (T90). Pre-stressor values for cortisol and glucose were not statistically different between groups. Plasma cortisol levels increased significantly and were of similar magnitude for both electroshock and the social stressor (mean ± SD for basal and final samples were: electroshock T60 = 65.47 ± 15.3, 177.0 ± 30.3; T90 = 54.8 ± 16.0, 196.2 ± 57.8; social stress T60 = 47.1 ± 9.0, 187.6 ± 61.7; T90 = 41.6 ± 8.1, 112.3 ± 26.8, respectively). Plasma glucose levels increased significantly for electroshock at both time points (T60 and T90), but only at T90 for the social stressor. Initial and final mean (± SD) values are: electroshock T60 = 52.5 ± 9.2, 115.0 ± 15.7; T90 = 35.5 ± 1.1, 146.3 ± 13.3; social stress T60 = 54.8 ± 8.8, 84.4 ± 15.0; T90 = 34.5 ± 5.6, 116.3 ± 13.6, respectively. Therefore, electroshock induced an increase in glucose more rapidly than did the social stressor. Furthermore, a significant positive correlation between cortisol and glucose was detected only at T90 for the social stressor. These results indicate that a fish species responds differently to different stressors, thus suggesting specificity of fish stress response to a stressor.

Nova et accuratissima tabula regionum ad Sinum Finnicum. Exactissimo delineata ac sumneo studio recognito ab Henrico de Leth.

[N. 1:1200000].

Tabvla Rvssiae ex autographo, quod delineandum curavit Foedor filius tzaris Boris desumta; et ad fluvios Dwinam, Zuchanam, aliaque loca, quantum ex tabulis et notitiis ad nos delatis fieri potuit, amplificata: ac magno domino, tzari , et magno duci Michäeli Foedorowits omnium Russorum, autcratori Wolodimeriae, Moscoviae et Novogardia. Tzari Cazaniae, tzari Astracaniae, tzari Siberiae, domino Plescoviae magno duci Smolenscoviae, Otweriae, Iugoriae, Permiae, Wiatkiae, Bulgariae etc: domino regionum Iveriae Kartalinie et Groesiniae tzari etc: dedicata ab Hesselo Gerardo M.DC.XIIII.

[N. 1:9000000].

Europa religionis christianae, morum, et pacis ac belli artium cultu omnium terrar: orbis partiu[m] praesantiß: juxta recent[issi]ma exempl: accurat[issi]ma excusa a Matth. Seuttero s. c. m. geogr.

[N. 1:27000000].

Nova mappa geographica Sueciae ac Gothiae regna ut et Finlandiae ducatum ac Lapponiam, cum provinciis minoribus, ob oculos ponens, cura et sumtibus Tobiae Conradi Lotter, chalcogr et geogr: Augustae Vindel.

Alareunassa privilegiomerkintä: Cum gratia et privil. s. r. i. vicariatq, in partibq Rheni, Sveviae, et juris Franconici.

Fasciculus physicae ejusdem illustriores definitiones, divisiones, atque nobiliores controversias axiomatibus nonnullis insertas brevibusque rationibus firmatas ac enodatas continens

Arkit: 4 arkintunnuksetonta lehteä, A-F8 G4.

Zetémata quaedam ex spatioso ac fructuoso philosophiae agro excerpta

Invokaatio: Cum Deo.

De theologiae natura, ad decretum venerandae facultatis theologicae, moderatore plurimum reverendo, amplissimo ac praeclarissimo dn. Abrahamo Thauvonio, s. s. theol. doctore & professore ord: p. t. magnifico rectore, boni ominis [!] causa, ante introductionem suam disputabit Petrus Bång Hels. In auditorio majore die 9. Decemb. anno 1665

Invokaatio: I.N.J.

Themata nonnulla theoretica simul ac practica duabus sectionibus inclusa

Invokaatio: Auxilio Jehovae.

Gene trio signatures as molecular markers to predict response to doxorubicin cyclophosphamide neoadjuvant chemotherapy in breast cancerpatients

In breast cancer patients submitted to neoadjuvant chemotherapy (4 cycles of doxorubicin and cyclophosphamide, AC), expression of groups of three genes (gene trio signatures) could distinguish responsive from non-responsive tumors, as demonstrated by cDNA microarray profiling in a previous study by our group. In the current study, we determined if the expression of the same genes would retain the predictive strength, when analyzed by a more accessible technique (real-time RT-PCR). We evaluated 28 samples already analyzed by cDNA microarray, as a technical validation procedure, and 14 tumors, as an independent biological validation set. All patients received neoadjuvant chemotherapy (4 AC). Among five trio combinations previously identified, defined by nine genes individually investigated (BZRP, CLPTM1,MTSS1, NOTCH1, NUP210, PRSS11, RPL37A, SMYD2, and XLHSRF-1), the most accurate were established by RPL37A, XLHSRF-1based trios, with NOTCH1 or NUP210. Both trios correctly separated 86% of tumors (87% sensitivity and 80% specificity for predicting response), according to their response to chemotherapy (82% in a leave-one-out cross-validation method). Using the pre-established features obtained by linear discriminant analysis, 71% samples from the biological validation set were also correctly classified by both trios (72% sensitivity; 66% specificity). Furthermore, we explored other gene combinations to achieve a higher accuracy in the technical validation group (as a training set). A new trio, MTSS1, RPL37 and SMYD2, correctly classified 93% of samples from the technical validation group (95% sensitivity and 80% specificity; 86% accuracy by the cross-validation method) and 79% from the biological validation group (72% sensitivity and 100% specificity). Therefore, the combined expression of MTSS1, RPL37 and SMYD2, as evaluated by real-time RT-PCR, is a potential candidate to predict response to neoadjuvant doxorubicin and cyclophosphamide in breast cancer patients.

Positiones philosophicae ex fragrantissimo philosophiae viridario carptìm collectae. Quas ex consensu amplissimae [et] venerandae facultatis philosophicae in Regiâ Academiâ Aboënsi. Sub praesidio, ... dn. Michaelis Gyllenstålpes, Wexionii, j. u. & philos: doct: pereximii, ll:m polit: & hist: prof: publ: celeberrimi, p. t. rectoris magnifici, moecenatis, praecept. ac promotoris quovis obsequii & honoris genere plurimùm prosequendi. Liberalis exercitij gratia, publicae eruditorum disquisitioni modestè sistit Daniel B. Rosander Smolandus s. r. m.tis stipendiarius. In auditorio majori ad diem 22. Novemb. anno 1654. Horis ab 8. antemeridianis

Variantti A.

De Sveo-Gothis; qui vixerunt sub periodo Erici I.mi. ad Ericum 2.dum: memorans quomodo restituti sunt primum ab Erico, mox tamen ac multos annos ab incertis ducibus vexati, at nunquam vera religione destituti

Variantti B.

De Sveo-Gothis; qui vixerunt sub periodo Erici I.mi. ad Ericum 2.dum: memorans quomodo restituti sunt primum ab Erico, mox tamen ac multos annos ab incertis ducibus vexati, at nunquam vera religione destituti

Variantti A.

De Sveo-Gothis, qvi vixerunt seculis post natum Christum, duobus primis: tradens fidei eorum articulos, ac conversorum nomina, sed cumprimis probans ecclesiae existentiam apud illos, seculo Christi secundo: docens item modum ejus tam propagatae in omnes provincias, quam auctae plurimum in provinciis cunctis

Variantti A.

Sanctus Ansgarius vulgo Svecorum Danorumque apostolus seu Vita Sancti Ansgarii olim â quodam pontificio latine scripta, ac postea ab alio in svecicum idioma translata: at hic rursus in latinum conversa, abbreviata et sobria censurâ theologicâ passim castigata, lucique publicae data

Invokaatio: I.N.J.