Genetic variability and limited clonality of Mycoplasma hyorhinis in pig herds

Mycoplasma hyorhinis is a common inhabitant of the upper respiratory tract and tonsils of pigs. Its role as a possible pathogen remains controversial. In order to gain more insight into the epidemiology and population structure of M. hyorhinis we genetically characterized 60 isolates by multi locus sequence typing (MLST). The M. hyorhinis strains originated from Swiss and German pig herds with knowledge on the clinical background. The MLST scheme of Tocqueville et al. (J. Clin. Microbiol. 2014) was optimized, primers for the six MLST gene fragments were newly designed to allow amplification and sequencing with a single protocol. A total of 27 ST were observed with the 60 strains, 26 of those were previously unknown types. Generally identical genotypes were observed within a farm but they differed between farms. The identical genotype was also observed in three different Swiss farms. On the other Hand different genotypes within a farm were found with three German farms. The Swiss isolates formed a distinct cluster but otherwise there was no geographical nor a clinical association with specific Clusters observed. Data shows a high variability of M. hyorhinis comparable to what is observed for Mycoplasma hyopneumoniae. Similar to this pathogen the population structure of M. hyorhinis also shows some limited clonality with predominant genotypes within an animal and a single farm but different ones between farms. The comparable population structure of M. hyopneumoniae and M. hyorhinis could indicate a similar evolution of the two species in the common pig host.

Contribution of Genetic Background and Clinical Risk Factors to Low-Trauma Fractures in Human Immunodeficiency Virus (HIV)-Positive Persons: The Swiss HIV Cohort Study.

Background.  The impact of human genetic background on low-trauma fracture (LTF) risk has not been evaluated in the context of human immunodeficiency virus (HIV) and clinical LTF risk factors. Methods.  In the general population, 6 common single-nucleotide polymorphisms (SNPs) associate with LTF through genome-wide association study. Using genome-wide SNP arrays and imputation, we genotyped these SNPs in HIV-positive, white Swiss HIV Cohort Study participants. We included 103 individuals with a first, physician-validated LTF and 206 controls matched on gender, whose duration of observation and whose antiretroviral therapy start dates were similar using incidence density sampling. Analyses of nongenetic LTF risk factors were based on 158 cases and 788 controls. Results.  A genetic risk score built from the 6 LTF-associated SNPs did not associate with LTF risk, in both models including and not including parental hip fracture history. The contribution of clinical LTF risk factors was limited in our dataset. Conclusions.  Genetic LTF markers with a modest effect size in the general population do not improve fracture prediction in persons with HIV, in whom clinical LTF risk factors are prevalent in both cases and controls.

Oil shale, potential environmental impacts and control technology /

Mode of access: Internet.

Development of treatment and control technology for refractory petrochemical wastes /

Mode of access: Internet.

A manual of the principal instruments used in American engineering and surveying /

Cover title: Gurley's manual: American engineers' and surveyors' instruments.

A manual of the principal instruments used in American engineering and surveying /

Cover title: Gurley's Manual. American engineers' and surveyors' instruments.

Ionizing radiations; their production, effects, and utilization, with special reference to food and packaging technology.

Mode of access: Internet.

Understanding genetic research and population-based studies /

"P 106"--P. [4] of cover.

Bibliography of New Mexico geology and mineral technology.

First vol. (through 1950) compiled by M.R. Burks and J.H. Schilling; 1951-55 and 1956-60 by C.F. and J.H. Schilling: 1961-65 by T. Ray; 1966-70 by M.A. and H.H. Koehn; 1971-75 by J.R. Wright and J.A. Russell; 1976-80 by Dana M. Adkins-Heljeson and Candace L. Holts.

Understanding genetic research and population-based studies /

"P 106"--P. [4] of cover.

Medicare's prospective payment system : strategies for evaluating cost, quality, and medical technology.

"OTA-H-262"--P. [4] of cover.