The Effects of Superovulation Prior to Mating and Zinc Supplementation on Milk Yields in Etawah-Grade Does

Thirty six Etawah-grade does (BW ranged from 20.4 to 44.2 kg and age ranged from 2.5 to 7 years) were used to study the effects of superovulation prior to mating and zinc supplementation on milk yields. The experimental does were assigned into a randomized block design with a 2 x 3 factorial arrangement. The first factor was PMSG (pregnant mare serum gonadotrophin) injection with 2 levels (0 IU/kg BW [nonsuperovulation–NSO] and 15 IU/kg BW [Superovulation–SO]). The second factor was zinc concentration in the diet with 3 levels (40 mg/kg DM [Z-40], 60 mg/kg DM [Z-60], and 80 mg/kg DM [Z-80]). Intravaginal sponge (60 mg medroxyprogesterone acetate) was applied for 14 days to synchronize estrus cycle. Twenty four  hours prior to sponge removal, PMSG was injected  to  stimulate superovulation. After  sponge removal,  5 experimental does  were mixed with 1 buck for natural mating. During  pregnancy  the experimental does were fed and  raised in   individual stables. Udder volume  was measured every other week from weeks 12 to 21 of  pregnancy.  During lactation  (one week to  5 months post partum) kids were separated from the does, and   milk was harvested by hand milking. Milk samples were taken every other week for determination of milk quality. The results  indicated that  superovulation increased udder differential growth  by 80% (822.85 vs 1481.25 cm3/head), milk yield by  32% (567.14 vs 746.52 g/head/day).  Zinc concentration  increased udder differential growth and milk yield. Milk productions for does receiving 40, 60 and 80 mg/kg DM were 565.68, 737.88 and 666.92 g/day, respectively.It was concluded that  superovulation and zinc supplementation  increased milk  yield in Etawah Grade does. (Animal Production 6(2): 86-94 (2004) Key Words:  Superovulation, Zinc, Milk  Yield, Etawah-Grade

The Utilization of Soybean Hulls as Energy Resources on Ration Local Sheep

An experiment was conducted to investigate the effects of soybean hulls as a ration in twenty seven rams. The animal had a mean of live weight of 12.863 ± 1.934 kg. Levels of soybean hulls were 0, 50, and 100 %  or 0,25, and 50 % in ration dry matter basis and rations were  iso-nitrogenous. The experiment were use Completely Randomized Design, data collected was analyzed using analysis of variance and polynomial orthogonal test.  Inclusion of soybean hulls in 50% ration dry matter had no effect on daily live weight gain (90.65±20.88 g), nitrogen, calcium and phosphor balances positive. However, dry matter consumption tended to increase linearly and as soybean hulls level increase in the ration (P<0.01). The digestible energy and NDF significantly decrease linearly (P<0.01), whereas intake of the energy was similar i.e. 122±0.39, 1.44±0.17, and 1.23±0.19 Mcal/day but NDF tended to increase for ration containing 0, 25, 50% of soybean hulls, respectively. The digestible crude protein is significantly quadratic (P<0.01), due to the release of energy and N are synchronized in 25% of soybean hulls in ration dry matter. It was concluded that soybean hulls can be used as a sources of energy and substitute for corn. (Animal Production 3(1): 5-11 (2001) Key Words: Local ram, soybean hulls, live weight gain, digestibility

Effect of Combination between CaCl2 Dosage and Electric Stimulation Period on Meat Quality Duck Adult

Research entitled “The effect of combination between CaCl2 dosage and length of electric stimulation on adult meat quality duck quality”. The aim was to know the effect of combination between CaCl2 and length of electric stimulation on quality. It was conducted at Technology of Animal Production Laboratory in Animal Science Faculty, Jenderal Soedirman University on June 2nd up to August 30rd 2002. Materials used in this research were 27 Tegal duck adult. Experimental design with Completely Randomized Design (CRD) with factorial 3 x 3 was performed in this research. The first factor was CaCl2 was dosage (a1=50cc; a2=100cc; and a3=150cc) and the second factor was length of electric stimulation period (b1=10 sec; b2=20 sec; and b3=30 sec). Each experiment was repeated three times, and variable of this research were pH, tenderness, WHC and CL. The result of research shows that CaCl2 dosage (50, 100, and 150 cc); length of electric stimulation and their interaction were gave no significant effect (P>0,05) on pH, tenderness, WHC and CL in adult duck meat. Conclusion of this research was CaCl2 dosage, electric stimulation period and their interaction gave the same effect on pH, tenderness, WHC and CL adult duck meat. (Animal Production 5(1): 25-34 (2003) Key word: Tenderness, WHC and CL, Duck, Meat, Electrical, Calsium Chlorida

The Synchronization of Estrous in Sheep and Goats

The review of estrous synchronization was biologically, hormonally, and laserly to studied in the sheep and goat. The observed parameters of the synchronization of estrous in sheep and goat were the onset and the length of estrous. The result of this research shows that the hormonal synchronization of estrous results in the presence of estrous in 30-42 hours after  the withdrawal of the sponges; while in goat it is in the average of  35.13±9.9 hours. Than the length of estrous in sheep was 30-48 hours and in goat the average was 36.00±14.96 hours. The synchronization of estrous by Laserpuncture results in the presence of estrous in goat 43.94-59.75 hours after the given laser beam (Animal Production 2(1): 1-8.Key Words: the synchronization of estrous, sheep, goat.

Egg Quality of Tegal Ducks in Intensive Keeping Feed with Different Levels of Methionine and Lancang (Atlanta sp.)

The purpose of this research is to study the effect of combination levels of methionine-lancang (Atlanta sp.) in diet on egg quality of Tegal ducks. Eighty-one Tegal ducks early production period were used in this research. The treatments were arranged in a factorial (3x3). The first factor was level of synthetic methionine supplement (M) comprised of  m1: 0,00%, m2: 0,05%, and m3: 0,10%. The second factor was level of lancang (L), i.e. l1: 0,0%, l2; 2,0%, and l3: 4,0%. The variables observed were eggshell-thickness, haugh unit, and egg index. The result showed that the average eggshell-thickness was 0,335 ± 0,002 mm, range between 0,317 to 0,337 mm. The average of haugh unit was 92,054 ± 2,906 range between 85,767 to 99,095, and average of egg index was 76,58 ± 2,91, range between 73,79-81,37%. The interaction of methionine-lancang did not have significant effect on eggshell thickness, haugh unit, and egg index during the first two months egg production. Level of methionine significantly different (P<0,05) the haugh unit, but did not affect eggshell thickness and egg index. Level of  lancang has affect (P<0,05) eggshell thickness significant, but did not significant affect the haugh unit, and egg index. It can be concluded that increasing of synthetic methionine in the diet until 0,10% can improve haugh unit, and increase of lancang until 4,0% can improve eggshell thickness. (Animal Production 4(2): 77-82 (2002) Key words : Egg Quality, Tegal Duck, Intensive, Methionine, Lancang

Genetic Characteristic of Indonesian Local Ducks Based on Single Nucleotide Polymorphism (SNP) Analysis in D-loop Region Mitochondria DNA

Abstract. The aim of the study was to know the genetic characteristic and polymorphysm of Indonesian local ducks including Magelang, Tegal, Mojosari, Bali and Alabio duck based on Single Nucleotide Polymorphism (SNP) analysis in D-loop region mtDNA. The long term aim was to set the spesific genetic marker based on SNP D-loop region mtDNA which could differentiate local ducks in Indonesia. In the future, it could be used as selection tool for local duck conservation, and refinement strategy as well as the improvement of genetic quality by utilizing the available native duck germplasm. There were 20 ducks for each duck population and were taken 3 ml of its blood as sample. DNA Isolation Kit high pure PCR template preparation (Geneaid) was uded for Genome DNA isolation.  Amplification with PCR technique used primer DL-AnasPF (L56) as forward and DL-AnasPR (H773) as reverse. Next, PCR product or amplicon were sequenced. Sequence result were analyzed with SNP technique and observed the similarity and difference of its nucleotide sequence between individual and population. The result of the study showed that genome DNA from local duck in Indonesia was successfully isolated. DNA fragment of 718 bp was amplified with primer pair of DL-AnasPF and DL-AnasPR. Nucleotide sequence was 469 nt and analyzed with SNP technique. It was compared with standard nucleotide sequence of Anas platyrhynchos (HM010684.1) in Gen Bank. The result of nucleotide sequence similarity percentage was 99.68±0.56%. Single Nucleotide Polymorphism D-loop region mtDNA Indonesian local duck was 0.32±0.56%.  Some SNP was found in Magelang duck C (Klawu blorok), F (Cemani black),  G (Gambiran), H (Jarakan kalung), I (Jowo plain) and K (Plain white) also Tegal duck 8, 1, 2, 5, 2, 8 and 2 SNP respectively. It could be concluded that polymorphic genetic characteristic similarity were existed in Indonesia local duck populations which was shown by its big standard deviation SNP in D-loop region mtDNA. Magelang duck with different feather color relatively more polymorphic to another local duck in Indonesia. Single Nucleotide Polymorphism which was achieved could be used as genetic marker that differentiate genetic characteristic of Indonesian local ducks.Key words:  genetic characteristic, local duck, Single Nucleotide Polymorphism (SNP), D-loop mtDNAAbstrak.  Penelitian ini bertujuan untuk mengetahui karakteristik genetik dan polimorfisme itik lokal Indonesia yaitu itik Magelang, Tegal, Mojosari, Bali dan Alabio berdasarkan analisis Single Nucleotide Polymorphism (SNP) daerah D-loop mtDNA. Tujuan jangka panjangnya adalah menetapkan marker atau penanda genetik berdasarkan SNP daerah D-loop mtDNA spesifik yang dapat membedakan itik-itik lokal yang ada di Indonesia. Selanjutnya digunakan sebagai  alat bantu seleksi untuk konservasi, pembibitan  dan pengembangbiakan itik lokal.  Populasi masing-masing jenis itik lokal yang digunakan sebanyak 20 ekor untuk diambil 3 ml sampel darahnya. Isolasi DNA genom menggunakan DNA Isolation Kithigh pure PCR template preparation (Geneaid). Amplifikasi dengan teknik PCR menggunakan pasangan primer DL-AnasPF (L56) sebagai forward dan DL-AnasPR (H773) sebagai reverse. Produk PCR atau amplikon yang diperoleh disekuensing. Hasil sekuensing dianalisis dengan teknik SNP dan diamati kesamaan dan perbedaan urutan nukleotida antar individu itik dan antar populasi.  Hasil penelitian menunjukkan bahwa DNA genom dari itik lokal di Indonesia berhasil diisolasi. Amplifikasi dengan teknik PCR berhasil memperoleh fragmen berukuran 718 bp. Urutan nukleotida hasil sekuensing sebesar 469 nt dianalisis dengan teknik SNP dan dibandingkan dengan urutan nukleotida standar dari itik Anas platyrhynchos (HM010684.1) yang ada di Gen Bank, diperoleh persentase kesamaan urutan nukleotid sebesar 99,68±0,56%. Single Nucleotide Polymorphism daerah D-loop mtDNA pada itik lokal di Indonesia sebesar 0,32±0,56%. Sejumlah SNP ditemukan pada itik Magelang C (Klawu blorok), F (Hitam cemani),  G (Gambiran), H (Jarakan kalung), I (Jowo polos) dan K (Putih polos) serta itik Tegal  masing-masing 8, 1, 2, 5, 2, 8 serta 2 SNP. Kesimpulan dari penelitian ini adalah terdapat karakteristik genetik yang polimorfik pada populasi itik lokal di Indonesia, ditunjukkan dengan adanya simpang baku SNP pada daerah D-loop mtDNA yang relatif besar. Itik Magelang dengan warna bulu yang berbeda relatif lebih polimorfik dibandingkan dengan itik lokal lainnya di Indonesia.  Single Nucleotide Polymorphism yang diperoleh dapat digunakan sebagai penanda genetik yang dapat membedakan karakteristik genetik yang dimiliki oleh itik lokal di Indonesia.Kata kunci:  karakteristik genetik, itik lokal, Single NucleotidePolymorphism (SNP),  D-loop mtDNA

Profile and Role of Woman Involvement in Dry System Pig Farming

This research was purposed to study the profile and role of woman involvement in dry system pig farming (pig housing with mattress from grass, pig manure, vegetable waste and kitchen waste).  The research was carried out from August to November 2003.  Pig housing areas were located on eight villages of Kertek District in Wonosobo Regency (Central Java).  The method applied was survey with intensive observation.  Sample were randomly selected, involved 180 respondents (15 percent of total pig farmers), while the locations of the sample were selected based on purposive sampling method.  The result showed that respondents were in productive age, with low formal education level. Most of all are Moslems (98%) and they have small family size.  The level of woman involvement in pig farming was high, approximately 85 percent.  They have other income source and they involve in pig farming to help their husbands in generating income.  Experience in the dry system pig farming was quite long (more than 10 years) and skill come from imitating their neighbours otherwise of autodidact. (Animal Production 6(1): 23-29 (2004) Key Words: Pig, Dry System, Woman Profile and Role

Behavioural Study of Timor Deer (Cervus timorensis) in PT Kuala Tembaga, Aertembaga Village, Bitung-North Sulawesi

The objective of this study was to observe the daily behavior of Timor deer (Cervus timorensis). The study was conducted in Aertembaga village, Bitung-North Sulawesi for 30 days. Deer were kept out in the conventional coconut plantations under extensive care system.  Seven Timor deers were used in this study. The daily behavior were observed for their activities; as grazing, ruminating, lying, urinating, defecating, vocalizing, working and other activities. A time sampling method was used to record the behavior activities and observations were repeated in five time.  The respective percentage of daily activities of Timor deer for grazing, ruminating, lying, working, standing,   defecating,  urinating,  approaching  and other activities were 31.17; 14.63; 13.54; 6.23; 7.55; 8.51; 3.96; 7.915 and 6.48%. (Animal Production 7(2): 121-126 (2005) Key Words: Care, Behavior, Timor deer, Extensive

Study Participation of Dairy Cattle Famers in Pollution Control Management to the Product of Milk

Based on activity, the population on dairy cattle, can be divided into two kinds i.e. pollution around the farm and pollution on the product of  milk. In order to eliminate the potency of the pollution, then, the manages to control it is urgently needed. The research was conducted by the farmers in banyumas Regency, Central Java Province, the has aids dairy cattle from government. The aim of the research was to know of participation to pollution control management on the product of milk. Survey method and descriptive analysis were used in this research. The technique of sampling used to collected data by Multy Stage Purposive Random Sampling from Sutrisno (1981). The independent variable of this research was social characteristic of the farmers i.e. mean of livelihood, income of cattlemen, participation of cattlemen on social institution and type of animal production, meanwhite, the dependent variable was the manages of pollution control the product of milk. To know the level of  participation control of pollution the milk product by crossing of the between variable table. Based on the analyses, it was found that the participation farmers to the manages to pollution control on the product of milk was in the level of  “good”. (Animal Production 1(2): 63-74 (1999) Key Words: Participation levels, pollution, milk.

The Various of Complete Feed Block for Dairy Cattle

Abstract. The effect of three forms of complete feed blocks (CFB) for dairy cattle was evaluated. The form of various CFB were cube, cylinder and ball.  The CFB was prepared from napier grass specific tolerance acid soils and concentrate in 50:50 ratio.  The research used experimental method with randomized block design.   Sixteen dairy cattle were used in this experiment.  There were four treatments and four replications used in this experiment.  The treatments consisted of R0= control ration, R1= cube CFB, R2= cylinder CFB and R3= ball CFB.  The treatment feeds were fed twice a day, at 6.00 am and 15.00 pm.  The amount of morning ration 34 kg of fresh napier grass and 5 kg of concentrate for the control ration, and the same amount of ration was also given in the afternoon feeding, therefore, the daily total fresh forage was 68 kg and concentrate was 10 kg.  Before feeding the forage was chopped in 5 cm length.  The complete feed block for the dairy cows was 10 kg for the morning ration and 10 kg for the afternoon ration, therefore, the daily total complete feed block was 20 kg.  The drinking water was available adlibitum.  The preliminary period was conducted for 2 weeks and data collection were conducted for 5 day of the end of study. The variables measured were dry matter and organic matter intake, dry matter digestibility and milk production.  These results showed that the control ration significantly affected with  ration all CFB form on dry matter and organic matter intake but did not significantly affect the dry matter digestibility and milk production. Key words : dairy cttle, complete feed block Abstrak.Penelitian ini bertujuan mengevaluasi pengaruh tiga pakan komplit bentuk cetak yaitu bentuk kubus, silinder dan bola yang diberikan pada sapi perah.   Pakan komplit bentuk cetak dibuat dari hijauan rumput gajah yang spesifik toleran tanah masam yang telah digiling dan bahan konsentrat dengan perbandingan 50:50.  Rancangan percobaan yang digunakan adalah rancangan acak kelompok.  Enam belas ekor sapi perah dikelompokkan menjadi 4 dengan 4 ulangan.  Empat perlakuan yang dicobakan yaitu R0= ransum kontrol, R1= pakan komplit cetak bentuk kubus, R2= pakan komplit cetak bentuk silinder, dan R3= pakan komplit cetak bentuk bola. Pakan perlakuan diberikan dua kali sehari pagi pukul 06.00 dan sore 15.00.  Jumlah pakan  yang diberikan pada sapi perah kontrol pagi sebanyak 34 kg rumput gajah segar dan 5 kg konsentrat demikian juga pemberian yang sama pada sore hari sehingga jumlah hijauan segar yang diberikan kepada ternak 68 kg dan konsentrat 10 kg.  Rumput gajah yang diberikan sudah dipotong-potong terlebih dahulu dengan panjang 5 cm.  Jumlah pakan komplit cetak untuk sapi perah perlakuan yang diberikan pagi jumlahnya sama dengan pemberian sore masing-masing sebanyak 10 kg sehingga jumlah pakan komplit cetak yang diberikan 20 kg.  Air minum diberikan adlibitum.  Periode preliminary dilakukan selama 2 minggu dan koleksi data dilakukan selama 5 hari periode akhir percobaan.  Variabel yang diamati adalah konsumsi bahan kering dan bahan organik pakan, kecernaan bahan kering pakan dan produksi susu.  Hasil penelitian menunjukkan bahwa ransum kontrol beda nyata dengan semua bentuk pakan komplit cetak pada konsumsi bahan kering dan bahan organik, akan tetapi tidak berbeda nyata dengan  kecernaan bahan kering dan produksi susu. Kata kunci : sapi perah, pakan komplit bentuk cetak

Contamination Rate of Lead in Pigeon (Columbia livia) at Semarang City

The aim of this research was to find out the lead concentration in some pigeon tissues in Semarang city. Fifty four pigeons 4-8 weeks old, which collected from 4 locations in Semarang were devided into 4 groups. Group I (15 pigeons) were obtained from the bird markets, group II (15 pigeons) were obtained from who sale fried birds, and group III (14 pigeons) were collected from the bird owners who look after the birds extensively (not in cages), then group IV (10 pigeons) were obtained from the bird owners who look after the birds intensively (in cages). All pigeons were euthanasia by chloroform then necropsied. A part of tissues eg: muscle, lung, liver and kidney were collected for lead examination by using Atomic Absorpsion Spectrophotometer (AAS). The result of the experiment showed that muscle, lung, liver and kidney pigeons were contaminated by lead, and lead concentration of kidney were higher (P< 0.05) than 0,1 ppm. Location of pigeons were not significant (P>0.05) with lead concentration in tissues pigeons. (Animal Production 2(2): 69-74 (2000) Key words : lead, pigeon, Semarang city

Grassing as potential factor in Gastrointestinal Nematodiasis in Goat in Banyumas District

Goat system manajemen like housing, feeding dan grassing have been studied on the diseases association in gastrointestinal nematodiasis infection. The method applied was survey on 150 goat (Jawarandhu) faeces sample in Banyumas District. The Research was carried out from July to September 2004. The result showed that  the nematode infection 1,54 times to goat if grassing and 88,62% infection in groups. Housing, Feeding have no risk and associated with disease infection. (Animal Production 6(2): 95-100 (2004) Key words : management system, disease association, nematode

Function Analysis of Milk Yield on Small Holder Dairy Cattle in Sumbang Banyumas Region

A survey on smallholder dairy cow had conducted in Banyumas region from November 8th 1999 up to February 8th 2000. Forty six respondents were involved in this study (as samples). Cobb - Douglas analysis was applied in this survey. From the study can be concluded that a small holder owned 3.02 heads of dairy cow (2.2  ST), 1.44 heads of lactating cow with 9.77 L/day milk production on average. The benefit of the backyard farming was RP.70.603,225 per month on average. Feeding was the biggest influence on benefit because feed cost was 65.81 percent from the production cost. The milk production function , by the equation: Y = 0,8958 X1⁰·⁰³³² X2 0.049 X30.168 X40.608 The four variables can explain 92.4 percent (R²=0,924)on the milk  production variation .It can be suggested that to improve milk production on dairy cows can be done by intensifying labour, and improving the forage feeding. (Animal Production 3(1): 12-19 (2001)Key word: Milk  production ,production function and benefit

The Quality of Hunted Sambar Deer (Cervus unicolor) Venison from East Kalimantan

Sambar deer (Cervus unicolor)  is the heaviest in its body weight and widest in its distribution of tropical deer. A Report by East Kalimantan governor indicated that no less than 5,000 wild sambar deer were slaughtered annually. In 1990 a pilot project of sambar deer farm was established and still under its development. Up to the present there is no data available on the nutritional values of sambar venison. The objective of the study was to determine the nutritional quality of wild sambar venison. Samples were collected from three traditional markets. Whitin 10 hours after being hunted, meat was sampled in three sites, front leg, back leg and saddle. The result showed that pH values of hunted sambar venison ranged from 6.18-6.46, but there were no differences in cutting sites. The moisture content was over 74%. Crude Protein, ash, fat and cholesterol (%DM) were 88.84-90.24, 3.86-4.14, 2.9-3.8, 0.24-0.27, respectively. Amino acids, fatty acids and minerals values were within the average of domesticated animals meat values, thought some values in sambar show a better performance. (Animal Production 5(1): 35-41 (2003) Key words: Sambar , Deer, Cervus unicolor, Venison, Meat

Correlation between Floor Space and Sanitation of Cage with Mastitis Disease Occurrence on The Dairy C

The objective of research was to know the correlation between floor space and sanitation of cage with mastitis disease occurance on the dairy cattle. Sixtiy infected mastitis disease dairy cows were used in the research in the Banyumas regency. Survey was applied in this experiment and Linier Multiple Regression was used. The result showed that there were correlations between floor space  and sanitation of cage with mastitis which follow the regression line Yi = 15,355 + 1,087 X1 – 0,249 X2 (Animal Production 2(1): 9-12Key Words: mastitis, floor space, and sanitation