Continuously measured 222Rn activity concentrations (daily means) in near surface air at Neumayer Station in the period 1995 through 2011

We report on continuously measured 222Rn activity concentrations in near-surface air at Neumayer Station in the period 1995-2011. This 17-year record showed no long-term trend and has overall mean ± standard deviation of (0.019 ± 0.012) Bq/m**3. A distinct and persistent seasonality could be distinguished with maximum values of (0.028 ± 0.013) Bq/m**3 from January to March and minimum values of (0.015 ± 0.009) Bq/m**3 from May to October. Elevated 222Rn activity concentrations were typically associated with air mass transport from the Antarctic Plateau. Our results do not support a relation between enhanced 222Rn activity concentrations at Neumayer and cyclonic activity or long-range transport from South America. The impact of oceanic 222Rn emissions could not be properly assessed but we tentatively identified regional sea ice extent (SIE) variability as a significant driver of the annual 222Rn cycle.

Return Migration in the Ecuadorian and Bolivian population: motivations, strategies and discourses

This article analyses the motivations for return migration among the Ecuadorians and Bolivians who, after living in Spain, returned to their countries of origin during the economic crisis that started in 2008. From the analysis of 22 interviews in-depth which took place in Ecuador and 38 in Bolivia to women, men and young people from migrant families, this decision-making process is shown to be embedded into a gendered dynamics of relationships. Particular detail is given to affective and economic elements that had an influence on the decision to return, as well as to the strategies deployed to project their readjustment back in origin. Males and females occupy differential positions within the family, work and social circle, their expectations being built in a gendered manner. Despite the fact migration has brought women greater economic power within the family group, their reintegration upon return redefines their role as main managers in the household and the dynamics that allow their social reproduction. Men, for their part, aspire to refresh their role as providers in spite of their frail labour position upon return. Social mobility for females is passed on through generations by a strong investment on education for their daughters and sons, while for males this mobility revolves around setting up family businesses and around their demonstrative abilities.

Radiological characterization of clay mixed red mud in particular as regards its leaching features

The reuse of industrial by-products such as red mud is of great importance. In the case of the building material industry the reuse of red mud requires a cautious attitude, since the enhanced radionuclide content of red mud can have an effect on human health. The natural radionuclide content of red mud from the Ajka red mud reservoir and the clay sample from a Hungarian brick factory were determined by gamma spectrometry. It was found that maximum 27.8% red mud content can be added to fulfil the conditions of the EU-BSS. The effect of heat treatment was investigated on a red mud-clay mixture and it was found that in the case of radon and thoron exhalation the applied heat reduced remarkably the exhalation capacities. The leaching features of red mud and different mixtures were studied according to the MSZ-21470-50 Hungarian standard, the British CEN/TS 14429 standard and the Tessier sequential extraction method. The Tessier method and the MSZ-21470-50 standard are suitable for the characterization of materials; however, they do not provide enough information for waste deposition purposes. To this end, we propose using the CEN/TS 14429 method, because it is easy to use, and gives detailed information about the material's behaviour under different pH conditions, however, further measurements are necessary.

Radiological and material characterization of high volume fly ash concrete

The main goal of research presented in this paper was the material and radiological characterization of high volume fly ash concrete (HVFAC) in terms of determination of natural radionuclide content and radon emanation and exhalation coefficients. All concrete samples were made with a fly ash content between 50% and 70% of the total amount of cementitious materials from one coal burning power plant in Serbia. Physical (fresh and hardened concrete density) and mechanical properties (compressive strength, splitting tensile strength and modulus of elasticity) of concrete were tested. The radionuclide content (226Ra, 232Th and 40K) and radon massic exhalation of HVFAC samples were determined using gamma spectrometry. Determination of massic exhalation rates of HVFAC and its components using radon accumulation chamber techniques combined with a radon monitor was performed. The results show a beneficial effect of pozzolanic activity since the increase in fly ash content resulted in an increase in compressive strength of HVFAC by approximately 20% for the same mass of cement used in the mixtures. On the basis of the obtained radionuclide content of concrete components the I -indices of different HVFAC samples were calculated and compared with measured values (0.27e0.32), which were significantly below the recommended 1.0 index value. The prediction was relatively close to the measured values as the ratio between the calculated and measured I-index ranged between 0.89 and 1.14. Collected results of mechanical and radiological properties and performed calculations clearly prove that all 10 designed concretes with a certain type of fly ash are suitable for structural and non-structural applications both from a material and radiological point of view.

Making British Indian Fictions, 1772-1823

This book examines fictional representations of India in novels, plays and poetry produced between the years 1772 to 1823 as historical source material. It uses literary texts as case studies to investigate how Britons residing both in the metropole and in India justified, confronted and imagined the colonial encounter during this period. The study will situate the texts in relation to the shifting colonial context and to the changing attitudes towards India within Britain in general and on the part of Britons who had experience of living in India, such as East India Company men or their wives and daughters, in particular. Moreover, it will analyse how this literature responded to the increasing influence of the subcontinent on metropolitan culture. This book, then, approaches fictional texts as case studies that illuminate trends taking place within Britain such as the growing consumption of Indian-style imported goods and the commoditisation of an Indian aesthetic within British visual culture. Whilst the book will utilise fictional portrayals to comment upon shifts in the relationship between coloniser and colonised and to discuss the cross-cultural influences between the metropole and the colonial periphery, it also outlines how literary production and print capitalism played a part in shaping depictions of the subcontinent and stereotypes of the colonial 'other'. The study will also examine how representations of the subcontinent in British art and scholarship were influenced by metropolitan literary and popular culture. At the same time it will look at how representations by metropolitan authors influenced early-nineteenth century depictions by British authors who resided in India.

Fecal androgen levels in common marmoset (Callithrix jacchus) males living in captive family groups

In captive common marmoset groups, the reproductive inhibition observed in subordinate female seems to be a result of olfactory, visual and behavioral cues from the dominant female. However, few studies have examined the relationship among adult males living in the same social group. These studies have shown that reproductive failure among peer males seems to be based on hormonal and behavioral mechanisms. New insights on sexual strategies in primates have been shown using fecal steroids, but so far no information is available for common marmoset males. In the present study, we evaluated the influence of light-dark cycle, age and reproductive condition on the profile of fecal androgens in males living in the same family group. Feces were collected from six fathers and six sons for androgen determination during the light phase of the 24-h cycle for eight days randomly distributed over a 4-week period. Androgen levels were determined by enzyme immunoassay technique. Adult sons showed higher androgen levels (166.97 ± 22.95 ng/g) than fathers (80.69 ± 44.38 ng/g) and juveniles (49.06 ± 23.15 ng/g; P < 0.05). No diurnal variation (P > 0.05) in fecal androgen profile was observed in adults or juveniles. No indication of androgen-mediated social competition between fathers and adult sons was demonstrable. These results provide basic information on fecal androgen profile useful to investigate the socioendocrinology of free-ranging common marmoset males and verify that, in contrast to daughters, the reproductive suppression of sons is not based on physiological inhibition of their gonads

Circadian variation with a diurnal bimodal profile on scent-marking behavior in captive common marmosets (callitrhix jacchus)

Scent-marking behavior is associated with different behavioral contexts in callitrichids, including signalizing a territory, location of feeding resources, and social rank. In marmosets and tamarins it is also associated with intersexual communication. Though it appears very important for the daily routine of the individuals, very few researchers have investigated distribution through the 24-h cycle. In a preliminary report, we described a preferential incidence of this behavior 2 h before nocturnal rest in families of common marmosets. We expand the data using 8 family groups (28 subjects), 8 fathers, 6 mothers, 8 nonreproductive adults (4 sons and 4 daughters), and 6 juvenile (3 sons and 3 daughters) offspring that we kept in outdoor cages under natural environmental conditions. We recorded the frequency of anogenital scent marking for each group during the light phase, twice a wk, for 4 consecutive wks, from March 1998 to September 1999. Cosinor test detected 24- and 8-h variations in 89.3% and 85.7% of the subjects, respectively, regardless of sex or reproductive status. The 8-h component is a consequence of the 2 peaks for the behavior, at the beginning and end of the light phase. Daily distribution of scent marking is similar to that others described previously for motor activity in marmosets. The coincident rhythmical patterns for both behaviors seem to be associated with feeding behavior, as described for callitrichids in free-ranging conditions, involving an increase in foraging activities early in the morning and shortly before nocturnal rest

Redes Sociais Pessoais de Idosos, Satisfação com as Relações Interpessoais e Confiança

Objetivos: O presente estudo tem como objetivo geral caracterizar as redes sociais pessoais dos idosos com idade igual ou superior a 65 anos, analisando-as segundo o nível de satisfação com as relações interpessoais e a confiança nos outros. Metodologia: Este é um estudo descritivo e correlacional, privilegiando a análise bivariada. Os dados foram recolhidos através do Instrumento de Análise da Rede Social Pessoal, IARSP-Idosos (Guadalupe, 2009; Guadalupe & Vicente, 2012) e de uma escala de avaliação da Satisfação com as Relações Interpessoais, construída para o efeito, e de uma questão relacionada com a Confiança. Participantes: A amostra é constituída por 446 indivíduos, maioritariamente do sexo feminino (n=285; 63,9%), com idades compreendidas entre os 65 e os 98 anos; a maioria tem filhos (n = 389; 87,2%), e cerca de 80,0% (n = 357) vivem na sua casa, sendo a zona de residência essencialmente rural (61,2%; n = 273). A maioria tem escolaridade (65,9%; n = 294), sobretudo ao nível do quarto ano (n= 226; 50,7%). Resultados: Os resultados demonstram que os idosos do sexo feminino, com ≤ 75 anos, casado/a ou em união de fato, com filhos, que vivem acompanhados, com o 4ª ano de escolaridade e que não registam qualquer corte relacional, são os que mais confiam nos outros. Registam-se diferenças nas características funcionais da rede segundo esta variável, o que não acontece nas estruturais, com a exceção da proporção das relações com técnicos (p = 0,042) e nas relacionais-contextuais. A confiança nas pessoas com quem se relaciona correlaciona-se de forma positiva e estatisticamente significativa com a satisfação com os filhos, com os netos, com outros parentes, com os amigos e com os vizinhos (p<0,001). Conclusões: Numerosas variáveis sociodemográficas não aparentam estar relacionadas com a confiança nas pessoas com quem os idosos se relacionam, nas múltiplas dimensões consideradas. Em contrapartida, as variáveis que aparecem relacionadas com a confiança, são aquelas que, de forma mais ou menos direta, estão igualmente associadas ao domínio pessoal. É de salientar que no que respeita a esta variável se verificam diferenças nas características funcionais da rede o que não acontece nas estruturais e nas relacionais-contextuais. As relações familiares de filhos, netos e outros parentes são as que mais se associam à confiança e ao apoio social percebido pelos idosos, o qual é complementado por outras relações interpessoais, designadamente as que são estabelecidas com amigos e vizinhos. / Objetives: This study has the general objective to characterize the personal social networks of the elderly aged over 65 years, analyzing them according to the level of satisfaction with interpersonal relationships and trust in others. Methodology: This is a descriptive and correlational study, focusing on bivariate analysis. Data were collected through the Personal Social Networks Analysis Tool, IARSP-Elderly (Guadalupe, 2009; Vicente & Guadalupe, 2012) and a scale measuring satisfaction with interpersonal relations, purpose built, and a question related to the trust. Participants: The sample includes 446 individuals, mostly female (n = 285; 63,9%), aged between 65 and 98 years old; most have sons/daughters (n = 389; 87,2%), and about 80,0% (n = 357) are living in their home, mostly in rural areas (61,2%, n = 273). The majority have education (65,9%, n = 294), especially at the level of the fourth year (n = 226; 50,7%). Results: The results show that the elderly female, with <= 75, married, with children, living together, with the 4th grade, and did not record any relational cut, are the ones that rely in the others. We found differences in the functional characteristics of the network according to this variable, what does not happen on the structural variables, with the exception of the proportion of relations with workers in social services (p = 0,042), and on the relational-contextual. The confidence in the people he meets, correlates positively and statistically significant satisfaction with the children, with grandchildren, other relatives, friends and neighbors (p <0,001) Conclusions: Numerous sociodemographic variables do not appear to be related to trust in the interpersonal relationship, in the multiple dimensions considered. In contrast, the variables which appear related to trust are those which are associated with the personal domain. It is noteworthy that we have found differences in the functional characteristics of the network but not in the structural and the relational-contextual. Family relationships of children, grandchildren and other relatives are the most associated to the confidence and social support perceived by the elderly, which is complemented by other interpersonal relationships, including those with established friends and neighbors.

Redes Sociais Pessoais de Idosos Segundo o Sexo dos Filhos

Objetivos: O presente estudo tem como objetivo analisar as redes sociais pessoais de idosos portugueses com filhos segundo o sexo da descendência e o sexo do/a idoso/a, relativamente às características estruturais, funcionais e relacionais-contextuais. Metodologia: Para a avaliação das variáveis em estudo foram utilizados o Instrumento de Análise da Rede Social Pessoal, versão para idosos (IARSP – Idosos) (Guadalupe, 2010; Guadalupe & Vicente, 2012) para avaliar as dimensões da rede social pessoal e um inquérito por questionário para caracterização da amostra a nível sociodemográfico e sociofamiliar. Participantes: A amostra é constituída por 498 idosos com filhos, com uma média de idades de 75 anos (DP=7,487), entre os 65 anos e os 98 anos, maioritariamente do sexo feminino (60,8%), casados ou em união facto (58,4%) e com escolaridade (70,7%). A maioria dos idosos inquiridos reside em aglomerado populacional (90,8%) e não usufrui de apoio de respostas sociais (78,1%). Resultados: Na análise separámos 3 subamostras: idosos com filhos de ambos os sexos (n=218; 43,7%), idosos com filhos do sexo masculino (n=125; 25,2%) e idosos com filhos do sexo feminino (n=155; 31,2%). Os idosos com filhas apresentam valores mais elevados no apoio material e instrumental (p = 0,046), apoio informativo (p = 0,018), companhia social (p = 0,018) e reciprocidade de apoio (p < 0,001). O tamanho da rede é menor no caso dos idosos com filhas comparativamente aos que têm filhos de ambos os sexos (p = 0,012). Analisando separadamente as redes das idosas e dos idosos da amostra, assinalamos que nas redes de idosos do sexo masculino apenas houve diferença na reciprocidade (p = 0,016), sendo menos recíprocas as redes da subamostra com filhos apenas do sexo masculino; por sua vez, nas redes das idosas houve diferenças no tamanho da rede (p = 0,015) e na frequência de contactos (p = 0,019) sendo maior nas idosas com filhos de ambos sexos; na proporção de relações de vizinhança na rede (p = 0,005), sendo menor nas idosas que têm filhos de ambos os sexos; no apoio informativo (p = 0,022) e na reciprocidade (p = 0,005) sendo menores nas idosas com filhos do sexo masculino. Conclusões: O nosso estudo revela que o sexo dos filhos influencia as redes sociais pessoais dos pais e mães idosos/as a nível funcional, estrutural e relacional-contextual, sobretudo no caso das mulheres idosas, uma vez que as redes das idosas apresentam diferenças nas três dimensões, o que não se verifica nos pais idosos, verificando-se também que os idosos com filhas do sexo feminino têm redes mais centradas nas relações familiares. / Goals: This study aims to analyze the personal social networks structural, functional and relational-contextual characteristics of Portuguese seniors with offspring, according to their sex. Methodology: To the variables evaluation, we have used the Personal Social Network Analysis Instrument, Elderly Version (IARSP – Elderly) (Guadalupe, 2010; Guadalupe & Vicente, 2012) in order to evaluate the dimensions of the personal social network, and a questionnaire for demographic description. Participants: Our sample has 498 seniors with offspring, with an average of 75 years of age (DP = 7,487), between 65 and 98 years old, mostly females (60,8%), married (58,4%) with education (70,7%).The majority live on agglomeration (90,8%) and does not have the support of social services (78,1%). Results: In this analysis we've 3 groups: seniors with sons and daughters (n = 218; 43,7%), seniors with male offspring (n = 125; 25,2%) and seniors with female offspring ( n = 155; 31,2%). Seniors with daughters only shows higher values on material and instrumental support (p = 0,046), informative support (p = 0,018), social company (p = 0,018) and reciprocity support (p < 0,001). The network size is smaller on senior's with offspring, comparing the ones with sons and daughters (p = 0,012). On a separate analysis of the male and female seniors sample, it should be noticed that on the male seniors network there's been a difference on reciprocity only (p = 0,016) being less reciprocal the networks from the group exclusively with male sons; on the other end, on the female seniors network with sons and daughters there's been a difference on the network size (p = 0,015) and on the contact frequency (p = 0,019) being smaller on the seniors with offspring from both sex; on the proportion relation of network neighborhood (p = 0,005), smaller on the female seniors with sons and daughters; on informative support (p = 0,022) and on the reciprocity (p = 0,005), being smaller on the female seniors with male offspring. Conclusions: Our study reveals that the male and female offspring influences the personal social networks of senior mothers and fathers on a functional, structural and context-relational level, mostly on women, once that their network presents differences in the three dimension, something that does not happen with senior fathers, with the verification that the seniors with daughters do have social networks that are more centered in family relations.

Funzioni BV e disuguaglianze isoperimetriche

All'interno della mia tesi verrà introdotta la teoria delle funzioni in R^{n} a variazione limitata (BV), seguendo le presentazioni di Lawrence C.Evans e Ronald F.Gariepy nel libro Measure Theory and Fine Properties of Functions e di Enrico Giusti nell'opera Minimal Surfaces and Functions of Bounded Variation. Le funzioni BV sono funzioni le cui derivate prime deboli sono misure di Radon, ossia misure di Borel regolari finite sui compatti. In particolare verranno anche analizzati gli insiemi E che hanno perimetro finito, ossia tali che la funzione indicatrice dell’insieme E sia una funzione BV. Nello specifico, nel primo capitolo verranno date le definizioni di funzioni BV e insiemi di perimetro finito, sia in una versione globale che in una locale, verrà enunciato un primo importante teorema per le funzioni BV e verrà analizzata la relazione tra funzioni di Sobolev e funzioni BV. Nel secondo capitolo, invece, verranno analizzate la semicontinuità inferiore, l'approssimazione con funzioni lisce e la compattezza di funzioni BV, mentre nel terzo capitolo verranno elencati alcuni risultati sulle funzioni BV riguardanti la Traccia, l'Estensione e la formula di Coarea. Infine, nel quarto ed ultimo capitolo, verranno studiate le disuguaglianze di Sobolev e Poincaré e le disuguaglianze isoperimetriche per funzioni BV.

Redes Sociais Pessoais de Idosos com e sem Filhos

Objetivos: O presente estudo tem como objectivo analisar as redes sociais e pessoais de idosos portugueses com filhos e sem filhos, relativamente às características estruturais, funcionais e relacionais-contextuais. Metodologia: Para a avaliação das variáveis em estudo foram utilizados: o Instrumento de Análise da Rede Social Pessoal, versão para idosos (IARSP – Idosos) (Guadalupe, 2010; Guadalupe & Vicente, 2012) para avaliar as dimensões da rede social pessoal; um inquérito por questionário para caracterização da amostra a nível sociodemográfico e sociofamiliar. Participantes: A amostra é constituída por 418 idosos, com uma média de idades de 76 anos (DP=7,62), entre os 65 anos e os 98 anos, maioritariamente do sexo feminino (63,9%), casados ou em união facto (51,0%) e com escolaridade (63,9,1%). A maioria dos idosos inquiridos vive com agregado familiar (80,4%) em zonas rurais (64,8) e não usufrui de qualquer tipo de apoio de resposta social (71,5%). Resultados: Os idosos com filhos são maioritários (n=364; 87,1%) e os sem filhos os minoritários (n=54; 12,9%) na nossa amostra. Dos que têm filhos, 29,9% (109) têm filhos únicos e 70,1% (255) têm mais do que um filho (67% com 1 ou 2 filhos), sendo os filhos maioritariamente de ambos os sexos (n=158; 43,4%). Os resultados sugerem que o facto de os idosos terem ou não terem filhos reflete-se sobretudo em diferenças nas características estruturais das redes. Os idosos com filhos apresentam uma média mais elevada no tamanho da rede, na proporção das relações de familiares, e satisfação da rede e reciprocidade de apoio (p ≤ 0,001), a nível das características funcionais. Já relativamente à proporção das relações de amizade, vizinhança na rede, são os idosos sem filhos que apresentam uma média substantivamente mais elevada (p ≤ 0,003) do que a outra subamostra, apresentando também uma maior durabilidade das relações interpessoais (p < 0,03). As correlações entre o número total de filhos/as com as características estruturais e funcionais da rede, indicam-nos que quanto maior é o número de filhos maior é o tamanho da rede, a proporção das relações familiares, o apoio emocional, a reciprocidade de apoio, a satisfação com a rede e com o suporte social. Conclusões: O nosso estudo revela que as redes sociais pessoais dos idosos diferenciam-se a nível estrutural segundo o facto de terem ou não filhos, mas também nalgumas variáveis funcionais. Os idosos com filhos apresentam redes mais alargadas e muito centradas nas relações familiares na rede (sensivelmente menos um terço) do que os idosos sem filhos. Os últimos apresentam redes menores (com cerca de 2 pessoas a menos) e mais investidas nas relações de amizade e de vizinhança (com proporções que representam mais do dobro destes vínculos). / Goals: The present study has the purpose to analyze the personal social networks of Portuguese elder with our without sons/daughters, in their structural, functional and contextual relations. Methodology: We used for the evaluation of the variables: The Personal Social Network Analysis Tool, regarding elderly people (IARSP – elderly people) (Guadalupe, 2010; Guadalupe & Vicente, 2012), to evaluate the personal social network dimensions; a survey through an inquiry to characterize the sample at sociodemographic and at sociofamily level. Participants: The sample includes 418 elderly with an average age of 76 years old (DP = 7,62) between 65 and 98 years old, mainly women (63,9%), married or living as a couple (51,0%) with education (63,9,1%). Most of the elderly people who answered the survey live in family household (80,4%) in rural areas (64,8) and do not benefit from any type of social services support (71,5%). Results: The majority is elderly people with sons/daughters (n=364, 87,1%) and the ones without are the minority (n= 54, 12,9%) in our sample. Within those who are parents, 29,9% (109) have only one child and 70,1% (255) have more than one child (67% with 1 or 2), and the children are mainly of both sexes (n= 158; 43,4%). The results suggest that the fact that the elderly have or not have children is reflected especially in differences in the structural characteristics of the networks. Elderly people with children have a higher average in the network size, in the proportion of family relationships, and satisfaction of the network and reciprocal support (p ≤ 0,001). Regarding the proportion of friendly relations, network neighborhood, are the elderly without children who have a substantially higher mean (p ≤ 0,003) than the other subsample, also featuring improved durability of interpersonal relationships (p < 0,003). The correlations between the total number of children/with the structural and functional characteristics of the network, indicate that the greater the number of children, the greater the size of the network, the proportion of family relationships, emotional, support, reciprocity support and satisfaction with social network. Conclusion: Our study reveals that the personal social networks of the elderly differ in the structural level, according to whether they have sons/daughters or not, but also in some functional variables. The elderly with children have networks much more broader and centered on family relationships (roughly one-third) than elderly without children. These elderly without children have smaller networks (about 2 fewer people) and more centered in relations of friendship and neighborhood (with proportions that are more than the double compared to the other subsample).

Development and application of sensitive genome-wide platforms to study the genetic and epigenetic (DNA methylation) makeup of gametes and early bovine embryos

Pour ce projet, nous avons développé une plateforme pour l’analyse pangénomique de la méthylation de l’ADN chez le bovin qui est compatible avec des échantillons de petites tailles. Cet outil est utilisé pour étudier les caractéristiques génétiques et épigénétiques (méthylation de l’ADN) des gamètes soumis aux procédures de procréation médicalement assisitée et des embryons précoces. Dans un premier temps, une plateforme d’analyse de biopuces spécifiques pour l’étude de la méthylation de l’ADN chez l’espèce bovine a été développée. Cette plateforme a ensuite été optimisée pour produire des analyses pangénomiques de méthylation de l’ADN fiables et reproductibles à partir d’échantillons de très petites tailles telle que les embryons précoces (≥ 10 ng d’ADN a été utilisé, ce qui correspond à 10 blastocystes en expansion). En outre, cet outil a permis d’évaluer de façon simultanée la méthylation de l’ADN et le transcriptome dans le même échantillon, fournissant ainsi une image complète des profils génétiques et épigénétiques (méthylation de l’ADN). Comme preuve de concept, les profils comparatifs de méthylation de l’ADN spermatique et de blastocystes bovins ont été analysés au niveau de l’ensemble du génome. Dans un deuxième temps, grâce à cette plateforme, les profils globaux de méthylation de l’ADN de taureaux jumeaux monozygotes (MZ) ont été analysés. Malgré qu’ils sont génétiquement identiques, les taureaux jumeaux MZ ont des descendants avec des performances différentes. Par conséquent, l’hypothèse que le profil de méthylation de l’ADN spermatique de taureaux jumeaux MZ est différent a été émise. Dans notre étude, des différences significatives entre les jumeaux MZ au niveau des caractéristiques de la semence ainsi que de la méthylation de l’ADN ont été trouvées, chacune pouvant contribuer à l’obtention de performances divergentes incongrues des filles engendrées par ces jumeaux MZ. Dans la troisième partie de ce projet, la même plateforme a été utilisée pour découvrir les impacts d’une supplémentation à forte concentration en donneur de méthyle universel sur les embryons précoces bovins. La supplémentation avec de grandes quantités d’acide folique (AF) a été largement utilisée et recommandée chez les femmes enceintes pour sa capacité bien établie à prévenir les malformations du tube neural chez les enfants. Cependant, plus récemment, plusieurs études ont rapporté des effets indésirables de l’AF utilisé à des concentrations élevées, non seulement sur le développement de l’embryon, mais aussi chez les adultes. Au niveau cellulaire, l’AF entre dans le métabolisme monocarboné, la seule voie de production de S-adénosyl méthionine (SAM), un donneur universel de groupements méthyles pour une grande variété de biomolécules, y compris l’ADN. Par conséquent, pour résoudre cette controverse, une forte dose de SAM a été utilisée pour traiter des embryons produits in vitro chez le bovin. Ceci a non seulement permis d’influencer le phénotype des embryons précoces, mais aussi d’avoir un impact sur le transcriptome et le méthylome de l’ADN. En somme, le projet en cours a permis le développement d’une plateforme d’analyse de la méthylation de l’ADN à l’échelle du génome entier chez le bovin à coût raisonnable et facile à utiliser qui est compatible avec les embryons précoces. De plus, puisque c’est l’une des premières études de ce genre en biologie de la reproduction bovine, ce projet avait trois objectifs qui a donné plusieurs nouveaux résultats, incluant les profils comparatifs de méthylation de l’ADN au niveau : i) blastocystes versus spermatozoïdes ; ii) semence de taureaux jumeaux MZ et iii) embryons précoces traités à de fortes doses de SAM versus des embryons précoces non traités.

De volta para os braços da rainha dos céus: migração, memória e festa em Caicó/RN

The present work has as an objective to seize up some particular meanings of the Feast of Sant Ana (The saint which represents the city of Caicó/RN), a central event of the calendar festive-religious of that city, looking for to perceive on how it combines with the symbolic poles sacred: profane through the social bonds that are present there performed and expressed through the social networking and of local belongings. Such bond ties reveal themselves through a very especial manner, through the migration of returning to the feast, when collectively are produced and updated knowledge and codes that reinforce the representation of the local belonging, nominated as being caicoense . We seek on perceiving how the many events of this feast (from a perspective of a religious order as well as laic) acting on a conclusive way to an identity of the re-meaning of that city, especially in its relation with the migrant natives: the sons and daughters that return to the arms of Sant Ana

Fecal androgen levels in common marmoset (Callithrix jacchus) males living in captive family groups

In captive common marmoset groups, the reproductive inhibition observed in subordinate female seems to be a result of olfactory, visual and behavioral cues from the dominant female. However, few studies have examined the relationship among adult males living in the same social group. These studies have shown that reproductive failure among peer males seems to be based on hormonal and behavioral mechanisms. New insights on sexual strategies in primates have been shown using fecal steroids, but so far no information is available for common marmoset males. In the present study, we evaluated the influence of light-dark cycle, age and reproductive condition on the profile of fecal androgens in males living in the same family group. Feces were collected from six fathers and six sons for androgen determination during the light phase of the 24-h cycle for eight days randomly distributed over a 4-week period. Androgen levels were determined by enzyme immunoassay technique. Adult sons showed higher androgen levels (166.97 ± 22.95 ng/g) than fathers (80.69 ± 44.38 ng/g) and juveniles (49.06 ± 23.15 ng/g; P < 0.05). No diurnal variation (P > 0.05) in fecal androgen profile was observed in adults or juveniles. No indication of androgen-mediated social competition between fathers and adult sons was demonstrable. These results provide basic information on fecal androgen profile useful to investigate the socioendocrinology of free-ranging common marmoset males and verify that, in contrast to daughters, the reproductive suppression of sons is not based on physiological inhibition of their gonads

Circadian variation with a diurnal bimodal profile on scent-marking behavior in captive common marmosets (callitrhix jacchus)

Scent-marking behavior is associated with different behavioral contexts in callitrichids, including signalizing a territory, location of feeding resources, and social rank. In marmosets and tamarins it is also associated with intersexual communication. Though it appears very important for the daily routine of the individuals, very few researchers have investigated distribution through the 24-h cycle. In a preliminary report, we described a preferential incidence of this behavior 2 h before nocturnal rest in families of common marmosets. We expand the data using 8 family groups (28 subjects), 8 fathers, 6 mothers, 8 nonreproductive adults (4 sons and 4 daughters), and 6 juvenile (3 sons and 3 daughters) offspring that we kept in outdoor cages under natural environmental conditions. We recorded the frequency of anogenital scent marking for each group during the light phase, twice a wk, for 4 consecutive wks, from March 1998 to September 1999. Cosinor test detected 24- and 8-h variations in 89.3% and 85.7% of the subjects, respectively, regardless of sex or reproductive status. The 8-h component is a consequence of the 2 peaks for the behavior, at the beginning and end of the light phase. Daily distribution of scent marking is similar to that others described previously for motor activity in marmosets. The coincident rhythmical patterns for both behaviors seem to be associated with feeding behavior, as described for callitrichids in free-ranging conditions, involving an increase in foraging activities early in the morning and shortly before nocturnal rest