Caracterização de frutos, divergência genética e estrutura espacial de jaboticabeiras nativas de fragmento florestal em Clevelândia - PR

To design strategies for the conservation and use of genetic resources of tree species such as jaboticaba tree, it is essential to make the characterization. In southwestern Paraná region, there are several forest fragments containing native jaboticaba tree (Plinia cauliflora), whose materials have broad potential for commercial orchards or breeding programs. As is the potential genetic diversity of a population to produce different genotypes, it would be able to start in such a characterization one of these fragments. The aim was to characterize fruits of jaboticaba tree (P. caulifora) of forest fragment kept in Clevelândia - PR for the presence of phenotypic variability, seeking to identify those superiors named for future selection as farming or male parent, as well as estimate genetic divergence between them, as a complementary tool for this purpose. Also, verify the regeneration and spatial distribution of the species. For the study was defined portion of a hectare (10.000 m²), with all individuals identified, mapped, with local coordinate system, and measured height and diameter. Fruits were characterized by sensory and biochemical characteristics in two years, 70 genotypes at 2013 and 56 at 2014, and of these 33 genotypes in both years. As a pre-selection criteria was adopted the choice of 20% of the genotypes that showed the highest frequency of superiority in the evaluated characteristics of the fruit. Genetic divergence among 33 genotypes per year was analyzed. The distribution pattern and spatial association was evaluated by Ripley's K function. It was classified for the first time the following ontogenetic stages of jaboticaba tree, by plant height, seedling (from 0.01 to 0.99 m), juvenile (1.0 to 4.99 m), immature (> 5.0 m, non-reproductive), adult (reproductive). It was also have been describe for the first time the naturally occurring juxtaposed seedlings, indicating polyembryony. The number of regenerating identified in the population (seedlings: n = 2163; juveniles: n = 330; immature: n = 59) was much larger than the number of adults (n = 132). The species showed reverse J-shaped size structure standard, with high concentration of regenerating. The regeneration distribution occurs in aggregate pattern and there is seedling-adult dependence, due seed dispersal and seedling emergence closest to mothers. The jaboticaba tree regeneration is sufficient to maintain the species for long term in this population, which should serve as reference to regeneration success for other studies of this important fruiting species from Ombrofile Mixed Forests. Has been pre-selected the jaboticaba trees 7, 42, 43, 47, 54, 91, 97, 104, 105, 118, 134, 153, 154, 157, 163, 169, 177, 186, 212, J7-01 and J7- 02, and 16 and 194 the ones that can now be selected by the superior characteristics of both cycles. It was recommended to carry out hybridization between genotypes 79 and 119, and 96 to 148. The quality of fruit analyzed showed potential for use as a dual purpose serving both in natura market or processing.

Dinâmica da regeneração natural de áreas em restauração pela transposição de solo superficial de cerrado e de floresta estacional

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Ecologia, Programa de Pós-Graduação em Ecologia, 2015.

ESTRUTURA DO COMPONENTE DE REGENERAÇÃO NATURAL E ARBÓREO DE DOIS MANGUEZAIS NO ESTADO DO PARANÁ

Esse estudo avaliou comparativamente os parâmetros fitossociológicos da regeneração natural e da vegetação arbórea em dois manguezais distintos no Estado do Paraná, um pertencente à Baía de Paranaguá (manguezal de Antonina) e outro à Baía de Guaratuba (manguezal de Guaratuba). Em cada área, três subáreas foram marcadas e amostradas cinco parcelas de 10 x 10 m para avaliação do componente arbóreo e quinze subparcelas de 1 x 1 m para avaliação da regeneração natural. Avicennia schaueriana , Rhizophora mangle e Laguncularia racemosa exibiram domínio e densidade relativa distintos entre as áreas. Manguezais de Antonina e Guaratuba diferiram tanto na estrutura da regeneração natural como no componente arbóreo. A Análise de Coordenadas Principais (PCoA) mostrou que as diferenças entre as áreas em relação às variáveis abióticas do solo estão fortemente relacionadas com os parâmetros estruturais do componente arbóreo dos manguezais. Os fatores edáficos dos manguezais têm grande importância na separação entre as áreas, já que reflete na capacidade de adaptação das plantas.

INFLUÊNCIA DA POSIÇÃO SOCIAL NAS RELAÇÕES MORFOMÉTRICAS DE Araucaria angustifolia

The morphometric relations allow describing dimensions of trees without prior knowledge of the age, it help the forest planning and implementation of silvicultural treatments, especially when needs to make sustainable use of forests. For this purpose, the aim of this study was to model and comparising the morphometric relations araucaria trees in social position dominant, codominant and dominated in native forest remnant, located in Lages, SC. A total of 294 trees distributed in dbh classes were intentionally selected inside of forest. In each tree was measured dbh, total height, bole height, crown diameter by eight radius, as well as the classification of social position. Simple and multiple linear regression models were used to describe the relation h/d, the proportion of the crown and formal crown in function of diameter at breast height with simple transformation, quadratic, cubic, inverse and logarithmic form. The analysis of covariance with dummy variables were used to describe the social position and tested the parallelism and slope of regression indicating need or not of the use independent regressions. The results indicated that even with great variability in the shape and size of the crown due to growth and competition process, the morphometric relations of araucaria can be accurately estimated by regression models. The relation h/d, proportion of the crown and formal crown can be described by individual model for social position dominant, codominant and dominant, or alternatively a single model with the use of dummy variables that differentiate trees group dominated for the relation h/d and formal crown. The proportion of crown presented difference in dimensions of the trees, being necessary to use dummy variable for each social stratus or use the individual models.

Adventitious rooting of conifers: influence of biological factors

Vegetative propagation of superior conifer trees can be achieved e.g. through rooted cuttings or rooted microshoots, the latter predominantly through in vitro tissue culture. Both techniques are used to achieve rapid multiplication of trees with favorable genetic combinations and to capture a large proportion of the genetic diversity in a single generation cycle. However, adventitious rooting of shoots (cuttings) is often not efficient due to various problems such as scarcity of roots and cessation of their growth, both of which limit the application of vegetative propagation in some conifer species. Many factors are involved in the adventitious rooting of shoots including physical and chemical ones such as plant growth regulators, carbohydrates, light quality, temperature and rooting substrates or media (reviewed by Ragonezi et al. 2010). The focus of this review is on biological factors, such as inoculations with Agrobacterium rhizogenes, plant- growth-promoting rhizobacteria and other endophytes, and mycorrhizal fungi, which were found to stimulate adventitious rooting. These microorganisms could contribute not only to adventitious root development but also help in protecting conifer plants against pathogenic microorganisms, facilitate acclimation and transplanting, and contribute to more sustainable, chemical-free forests.

GuiaTreeKey, a multi-access electronic key to identify tree genera in French Guiana

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Anti-nociceptive and anti-inflammatory potentials of fractions from the extract of Tetracarpidium conophorum leaf in rats and mice

Tetracarpidium conophorum (TC) (Euphorbiaceae) is a perennial woody climbing shrub in low bush forest of some parts of West Africa and used among the natives for relief of ailments accompanying pain and inflammation. In this study, the analgesic and anti-inflammatory effects of the methanolic extract (METC) and fractions (ethyl acetate, F1 and n-hexane, F2) of Tetracarpidium conophorum leaf were evaluated in rat and mice. The analgesic activity was evaluated using acetic acid-induced writhing, formalin-induced paw licking and hot plate test models. Carrageenan-induced paw oedema was used to assess anti-inflammatory activity in rats. The mechanism of action of (TC) was explored by the use of naloxone, a non-selective opioid receptor blocker. The highest analgesic effect was observed in F2 extract at 57.21% inhibition and was further studied on various analgesic and anti-inflammatory models in graded doses. F2 significantly inhibited the late phase of formalin-induced paw licking and prolong hot plate latency at 55±1°C. The n-hexane fraction also significantly inhibited carrageenan-induced paw oedema in rats at 100 and 200mg/kg doses significantly (p< 0.001) and reduced paw licking response by 85.08% compared with control. Naloxone, an opioid receptor antagonist, did not significantly affect the changes observed with n-hexane fraction, thus ruling out the possibility of the involvement of opioid receptors in the analgesic actions of Tetracarpidium conophorum. Phytochemical screening showed that the leaf extracts contain alkaloids, tannins, saponins and cardenolides. The investigations showed that Tetracarpidium conophorum possesses significant anti-nociceptive and anti-inflammatory activities that should be explored.

The dynamics of Brazilian protozoology over the past century

Brazilian scientists have been contributing to the protozoology field for more than 100 years with important discoveries of new species such as Trypanosoma cruzi and Leishmania spp. In this work, we used a Brazilian thesis database (Coordination for the Improvement of Higher Education Personnel) covering the period from 1987-2011 to identify researchers who contributed substantially to protozoology. We selected 248 advisors by filtering to obtain researchers who supervised at least 10 theses. Based on a computational analysis of the thesis databases, we found students who were supervised by these scientists. A computational procedure was developed to determine the advisors’ scientific ancestors using the Lattes Platform. These analyses provided a list of 1,997 researchers who were inspected through Lattes CV examination and allowed the identification of the pioneers of Brazilian protozoology. Moreover, we investigated the areas in which researchers who earned PhDs in protozoology are now working. We found that 68.4% of them are still in protozoology, while 16.7% have migrated to other fields. We observed that support for protozoology by national or international agencies is clearly correlated with the increase of scientists in the field. Finally, we described the academic genealogy of Brazilian protozoology by formalising the “forest” of Brazilian scientists involved in the study of protozoa and their vectors over the past century.

Dinâmica de uma floresta tropical manejada na Amazônia oriental

Dissertação (mestrado)—Universidade de Brasília, Faculdade de Tecnologia, Departamento de Engenharia Florestal, 2016.

EVALUATION AND MONITORING OF ASH (FRAXINUS SPP.) TOLERANT TO LONG-TERM EMERALD ASH BORER (AGRILUS PLANIPENNIS [COLEOPTERA: BUPRESTIDAE]) EXPOSURE

Invasive insects that successfully establish in introduced areas can significantly alter natural communities. These pests require specific establishment criteria (e.g. host suitability) that, when known, can help quantify potential damage to infested areas. Emerald ash borer (Agrilus planipennis [Coleoptera: Buprestidae]) is an invasive phloem-feeding pest which is responsible for the death of millions of ash trees (Fraxinus spp. L.). Over 200 surviving ash trees were previously identified in the Huron-Clinton Metroparks located in southeast Michigan. Trees were assessed over a four year period and a hierarchical cluster analysis was performed on dieback, vigor, and presence of signs and symptoms, in order to place trees into one of three tolerance groups. The clustering of trees with different responses to emerald ash borer attack suggests that there are different tolerance levels in North American ash trees in southeastern Michigan, and these groups were designated as apparently tolerant, not tolerant and intermediate tolerance. Adult landing rates and evidence of adult emergence were significantly lower in the apparently tolerant group compared with the not tolerant group, but larval survival from eggs placed on trees did not differ between tolerance groups. Therefore, it appears that apparently tolerant trees survive because they are less attractive to adult beetles which results in fewer eggs being laid on them. Trees in the apparently tolerant group remained of higher vigor over the four years of the study. North American ash may survive the emerald ash borer epidemic due to natural variation and inherent resistance regardless of the lack of co-evolutionary history with emerald ash borer.

GENETIC VARIATION, LOCAL ADAPTATION AND POPULATION STRUCTURE IN NORTH AMERICAN RED OAK SPECIES, QUERCUS RUBRA L. AND Q. ELLIPSOIDALIS E. J. HILL

Forest trees, like oaks, rely on high levels of genetic variation to adapt to varying environmental conditions. Thus, genetic variation and its distribution are important for the long-term survival and adaptability of oak populations. Climate change is projected to lead to increased drought and fire events as well as a northward migration of tree species, including oaks. Additionally, decline in oak regeneration has become increasingly concerning since it may lead to decreased gene flow and increased inbreeding levels. This will in turn lead to lowered levels of genetic diversity, negatively affecting the growth and survival of populations. At the same time, populations at the species’ distribution edge, like those in this study, could possess important stores of genetic diversity and adaptive potential, while also being vulnerable to climatic or anthropogenic changes. A survey of the level and distribution of genetic variation and identification of potentially adaptive genes is needed since adaptive genetic variation is essential for their long-term survival. Oaks possess a remarkable characteristic in that they maintain their species identity and specific environmental adaptations despite their propensity to hybridize. Thus, in the face of interspecific gene flow, some areas of the genome remain differentiated due to selection. This characteristic allows the study of local environmental adaptation through genetic variation analyses. Furthermore, using genic markers with known putative functions makes it possible to link those differentiated markers to potential adaptive traits (e.g., flowering time, drought stress tolerance). Demographic processes like gene flow and genetic drift also play an important role in how genes (including adaptive genes) are maintained or spread. These processes are influenced by disturbances, both natural and anthropogenic. An examination of how genetic variation is geographically distributed can display how these genetic processes and geographical disturbances influence genetic variation patterns. For example, the spatial clustering of closely related trees could promote inbreeding with associated negative effects (inbreeding depression), if gene flow is limited. In turn this can have negative consequences for a species’ ability to adapt to changing environmental conditions. In contrast, interspecific hybridization may also allow the transfer of genes between species that increase their adaptive potential in a changing environment. I have studied the ecologically divergent, interfertile red oaks, Quercus rubra and Q. ellipsoidalis, to identify genes with potential roles in adaptation to abiotic stress through traits such as drought tolerance and flowering time, and to assess the level and distribution of genetic variation. I found evidence for moderate gene flow between the two species and low interspecific genetic differences at most genetic markers (Lind and Gailing 2013). However, the screening of genic markers with potential roles in phenology and drought tolerance led to the identification of a CONSTANS-like (COL) gene, a candidate gene for flowering time and growth. This marker, located in the coding region of the gene, was highly differentiated between the two species in multiple geographical areas, despite interspecific gene flow, and may play a role in reproductive isolation and adaptive divergence between the two species (Lind-Riehl et al. 2014). Since climate change could result in a northward migration of trees species like oaks, this gene could be important in maintaining species identity despite increased contact zones between species (e.g., increased gene flow). Finally I examined differences in spatial genetic structure (SGS) and genetic variation between species and populations subjected to different management strategies and natural disturbances. Diverse management activities combined with various natural disturbances as well as species specific life history traits influenced SGS patterns and inbreeding levels (Lind-Riehl and Gailing submitted).

Emergetic evaluation of cattle rearing in a montado farm

O Montado, em Portugal, é um complexo sistema silvopastoril de uso da terra, tipicamente Mediterrânico, com diversos estratos de vegetação, incluindo sobreiro e azinheira em várias densidades, onde é frequente a criação de gado. Esta actividade pecuária beneficia das pastagens no sob-coberto, de algumas espécies arbustivas e também das bolotas que caem do coberto arbóreo, contribuindo para evitar a invasão da pastagem por matos. No entanto, dependendo da sua gestão, este gado pode comprometer a regeneração do sistema. Nos últimos 20 anos, os subsídios no âmbito da Política Agrícola Comum da União Europeia têm promovido a criação de gado bovino em detrimento de outras espécies e raças mais leves, bem como a intensificação desta produção. Esta intensificação pode impossibilitar a regeneração natural das árvores ameaçando o equilíbrio do Montado. Por esta razão é necessária uma avaliação focada na criação de gado bovino e nos seus impactos sobre o sistema. O objectivo deste estudo foi obter uma melhor compreensão do funcionamento de uma exploração silvopastoril num sistema de Montado, através da aplicação do Método de Avaliação Emergética e do cálculo de índices emergéticos. Pretende-se assim compreender a melhor forma de o gerir, bem como conceber estratégias que maximizem o fluxo de emergia na exploração. Uma comparação deste método com a avaliação económica permitiu perceber em que aspectos esta pode ser complementada pelo método da avaliação emergética. O método da avaliação emergética permite a avaliação de sistemas multifuncionais complexos à escala de uma exploração individual, fornecendo informação extra em relação à avaliação económica como a renovabilidade dos inputs do sistema, ou a quantidade de fluxos livres da natureza que é valorada por preços de mercado. Este método permite a integração das emternalidades e das externalidades à contabilização económica, transformando uma avaliação tendencialmente separada do seu sistema mais vasto, numa avaliação de um sistema em conexão com aqueles mais vastos nos quais se integra; Abstract: The Montado, in Portugal, is a complex silvo-pastoral system of land use, typically Mediterranean, with different strata of vegetation, including cork and holm oaks in various densities, and where cattle rearing is common. This stockfarm benefits from the herbaceous layer under the trees, as well as from some species in the shrub layer, and also from the acorns faling down from the tree cover, while contributing to prevent the invasion of pastures by shrubs. Nevertheless, depending on its management, livestock can affect the system regeneration. Over the past 20 years, subsidies of the European Union's common agricultural policy have promoted the cattle rearing at expense of other lighter species and breeds, as well as its intensification. This intensification may impair the natural regeneration of trees threatening the balance of the Montado. Therefore an assessment focused on cattle and their impact on the system is required. The purpose of this study was to obtain a better understanding of the functioning of a silvo-pastoral farm in a Montado system, by applying the emergy evaluation method and through the calculation of emergy indices. It is intended to understand the best way to manage and design strategies that maximize the emergy flow on the farm. A comparison of this method with the economic evaluation allowed to realize in what aspects it can be complemented by the emergy evaluation method. The emergy evaluation method alows the assessment of complex multi-functional systems at the scale of an individual farm, providing extra information in relation to economic avaluation as the renewability of the inputs to a system and the amount of free flows of nature that is valued by market prices. This method allows the integration of the emternalities and the externalities to the economic accounting, transforming an evaluation tended separated from its wider system, in an evaluation of a system in connection with the larger ones on which it is incorporated.

Reducing competition in agroforestry by pruning native trees.

The degree to which pruning helps reestablish balance in agroforestry was assessed in a system established in São Carlos, São Paulo, Brazil, in 2008. Seven native tree species were planted at a density of 600 trees/ha in five strips of three rows each, and annual crops were cultivated in the 17-m crop strips between the tree strips. Competition was established after 35 months, decreasing the aboveground biomass production of corn planted close to the trees. An assessment of black oats in the dry season following tree pruning showed that the proximity of trees caused reductions in plant and panicle density, aboveground biomass production, number of grains per panicle and grain weight. Because pruning was not sufficient to maintain crop yields, tree thinning is recommended in order to minimize competition and restore conditions for adequate crop production.

Analysis of spatial variation of tree heights in an urban area using LiDAR data.

Estimating with greater precision and accuracy the height of plants has been a challenge for the scientific community. The objective this study is to evaluate the spatial variation of tree heights at different spatial scales in areas of the city of Recife, Brazil, using LiDAR remote sensing data. The LiDAR data were processed in the QT Modeler (Quick Terrain Modeler v. 8.0.2) software from Applied Imagery. The TreeVaW software was utilized to estimate the heights and crown diameters of trees. The results obtained for tree height were consistent with field measurements.

Proposed REDD+ project for the Sundarbans : legal and institutional issues

Sundarbans, a Ramsar and World Heritage site, is the largest single block of tidal halophytic mangrove forest in the world covering parts of Bangladesh and India. Natural mangroves were very common along the entire coast of Bangladesh. However, all other natural mangrove forests, including the Chakaria Sundarbans with 21,000 hectares of mangrove, have been cleared for shrimp cultivation. Against this backdrop, the Forest Department of Bangladesh has developed project design documents for a project called ‘Collaborative REDD+ Improved Forest Management (IFM) Sundarbans Project’ (CRISP) to save the only remaining natural mangrove forest of the country. This project, involving conservation of 412,000 ha of natural mangrove forests, is expected to generate, over a 30-year period, a total emissions reduction of about 6.4 million tons of CO2. However, the successful implementation of this project involves a number of critical legal and institutional issues. It may involve complex legal issues such as forest ownership, forest use rights, rights of local people and carbon rights. It may also involve institutional reforms. Ensuring good governance of the proposed project is very vital considering the failure of the Asian Development Bank (ADB) funded and Bangladesh Forest Department managed ‘Sundarbans Biodiversity Conservation Project’. Considering this previous experience, this paper suggests that a comprehensive legal and institutional review and reform is needed for the successful implementation of the proposed CRISP project. This paper argues that without ensuring local people’s rights and their participation, no project can be successful in the Sundarbans. Moreover, corruption of local and international officials may be a serious hurdle in the successful implementation of the project.