Western blotting as a tool for the serodiagnosis of farmer's lung disease: validation with Lichtheimia corymbifera protein extracts.

Electrosyneresis and double diffusion are immunoprecipitation techniques commonly used in the serological diagnosis of Farmer's lung disease (FLD). These techniques are reliable but lack standardization. The aim of this study was to evaluate Western blotting for the serodiagnosis of FLD. We carried out Western blotting with an antigenic extract of Lichtheimia corymbifera, an important aetiological agent of the disease. The membranes were probed with sera from 21 patients with FLD and 21 healthy exposed controls to examine the IgG antibody responses against purified somatic antigens. Given the low prevalence of the disease, 21 patients could be considered as a relevant series. Four bands were significantly more frequently represented in membranes probed with FLD sera (bands at 27.7, 40.5, 44.0 and 50.5 kDa) than those probed with control sera. We assessed the diagnostic value of different criteria alone or in combination. The diagnostic accuracy of the test was highest with the inclusion of at least two of the following criteria: at least five bands on the strip and the presence of one band at 40.5 or 44.0 kDa. Sensitivity, specificity and positive and negative predictive values were all 81%, and the odds ratio was 18.06. Inclusion of bands of high intensity diminished rather than improved the diagnostic value of the test. We concluded that Western blotting is a valuable technique for the serodiagnosis of FLD. The industrial production of ready-to-use membranes would enable the routine use of this technique in laboratories, and provide reliable and standardized diagnostic results within a few hours.

Quantitative elemental analysis of dry-ashed bark and wood samples of birch, spruce and pine from south-western Finland using PIXE

A total of over 200 different samples of bark and wood of Silver birch, Norway spruce and Scots pine were analysed. Samples were taken from several areas in western Finland, some with known sources of atmospheric heavy metal emission (Harjavalta, Ykspihlaja). Also analytical data for pine needles from some sites are reported. The chemical analyses were performed by thick-target particle-induced X-ray emission (PIXE) spectrometry after preconcentration by dry ashing of samples at 550oC. The following elements were quantified in most of the samples: P, S, K, Ca, Mn, Fe, Ni, Cu, Zn, Rb, Sr, Cd, Ba and Pb. The ash percentage and the chemical composition of ashes of different wood materials were also obtained, as dry ashing was used in the analytical procedure. The variations in elemental concentrations in wood and bark of an individual tree, expressed as RSDs, were mostly in the range 10 – 20 %. For several trees of the same species sampled from small areas (< 1 ha), the variations in elemental concentrations were surprisingly high (RSDs 20 – 50 %). In the vicinity of metal plants, effects of strong atmospheric heavy metal pollution (pollution factor above 100) were observed in pine bark. The increase of heavy metal content in wood samples from the same sites was quite small. Elemental concentrations in ashes of bark and wood, from areas with no local source of atmospheric pollution, were relatively uniform. Based on this observation an alternative way of demonstrating atmospheric pollution of tree bark is discussed.

Upper Eocene-Lowermost Miocene charophyte succession in the Ebro Basin (Spain). Contribution to the charophyte biozonation in Western Europe

A charophyte succession has been established in nineteen sections and eight isolated outcrops from the Ebro Basin, ranging from the Priabonian ro the Aquitanian. Most assemblages were recovered fmm continuous sections and their abundance and diversity allow to define a new zonal scheme for the Upper Eocene-Lower Miocene of Europe. The new zonation includes nine subdivisions, based on the distribution of thirty-two species and directly correlated with the mammal standard levels. Changes in diversity, occurring mainly in the Middle and Upper Oligocene, have been related to climatíc variations. A new species, Chara sp. A. is also described and figured.

Epibiotic bryozoans on deep-water seleractinian corals from the Catalonia slope (western Mediterranean, Spain, France)

The bryozoan fauna growing on deep-water corals (Lophelia, Madrepora) from the upper-slope of Catalonia (Blanes and Banyuls-sur-mer: NW Mediterranean Sea) was studied. Among the 36 species recorded, a new species, Escharella acuta sp. nov., and a new subspecies, Escharina dutertrei protecta ssp. nov., are described; five other species have been rarely reported or were unknown from the Mediterranean Sea (Copidozoum exiguum, Amphiblestrum flemingii, Schizomavella neptuni, Smittina crystallina, Phylactellipora eximia) . This epibiotic bryozoan fauna differs clearly from shallow-water assemblages and comprises a greater proportion of boreo-atlantic species.

Introgressive hybridization of threatened European tree frogs (Hyla arborea) by introduced H. intermedia in Western Switzerland

Hybridization by introduced taxa is a major threat to native species. Characterizing human introductions is thus one of the missions of conservation geneticists. Here we survey a declining population of the regionally endangered European tree frog (Hyla arborea) in the Grangettes natural reserve (Rhone valley, Western Switzerland), where previous evidence indicated human introduction of the Italian taxon H. intermedia. We combined fast-evolving mitochondrial and nuclear markers and an extended sampling to conduct population genetic analyses of the Grangettes and putative source areas. We show that the Grangettes population is a hybrid swarm, with all individuals featuring recent nuclear admixture and mitochondrial DNA of introduced H. intermedia, most likely of proximate south Alpine origin. In contrast, H. arborea and H. intermedia hardly introgress in their natural parapatric ranges, consistent with an advanced reproductive isolation. Thus, potential hybrid incompatibilities may account for the strong decline of this population, despite important conservation efforts. Although their hybrid nature makes them a priori unworthy of any protection, we propose specific measures to recover local H. arborea gene pool and preserve tree frogs in the Grangettes, the last population remaining from this heavily impacted part of the Alps.

Feeding habits of Ophichthus rufus (Anguilliformes, Ophichthidae) in the western Mediterranean

Ophichthus Rufus is a Mediterranean benthic fish with nocturnal habits. It lives in sand or mud bottoms at depths between 50 and 150m. Until now, nothing about its feeding habits was known. Stomach contents of 689 individuals, collected between 1985 and 1987, were analysed. The diet is basically composed of benthic organisms, among which the decapods processa canaliculata and Alpheus glaber and the teleost fish Callionymus maculatus are prominent. Young of both sexes and adult males have a euryphagic carnivorous diet, whereas adult females are closer to a stenophagic piscivorous diet

La surveillance bancaire belge de 1935 façonnée sur le modèle suisse: un discret transfert de politique publique?

Suite aux graves crises bancaires et financières qui ont secoué la plupart de sociétés occidentales au début des années 1930, on assiste à un foisonnement de réglementations publiques sur les activités bancaires. Parmi les très nombreux pays qui mettent en place des législations bancaires, l'on compte notamment la Suisse et la Belgique. Plus précisément, la loi fédérale suisse sur les banques de novembre 1934 précède de quelques mois l'arrêté royal belge no 185 de juillet 1935. Alors que le contexte historique et l'élaboration respective de chacune de ces réglementations ont déjà fait l'objet de plusieurs analyses, la comparaison et la question des éventuels liens entre ces deux régimes de surveillance bancaire très proches sont encore largement inexplorées. Une analyse détaillée du processus d'élaboration de la réglementation bancaire belge révèle que la loi suisse a joué un rôle de modèle mis en avant en premier chef par les représentants du monde bancaire. Cette influence helvétique a contribué à façonner la régulation bancaire dans une perspective libérale ; elle répondait ainsi à deux besoins essentiels: limiter l'interventionnisme étatique au minimum, et court-circuiter le pouvoir de la banque centrale. Cette parenté de naissance entre les deux lois bancaires trouve son pendant dans les activités clés deux organismes de surveillance créés pour l'occasion, la Commission fédérale des banques à Berne et la Commission bancaire à Bruxelles. Disposant toutes deux de moyens d'actions limités, ces deux instances ont connu des parcours analogues au cours de leurs premières années d'activité.

FDI from European Union to Western Balkan Countires: is the economic development being intensified in the region?

La inversió estrangera directa és important a causa dels seus avantatges per al país d'acollida, per exemple augmentarà la competitivitat i s’intensifica el desenvolupament. Els avantatges són significatives als països dels Balcans occidentals que s'enfronten a les dificultats de la competència i el creixement més baix de la UE-27. El treball analitza el tipus dominant de la IED de la UE-27 a la regió dels Balcans Occidentals a escala nacional i sectorial per formar les implicacions sobre el futur creixement de la competitivitat i la intensitat del desenvolupament econòmic. Es troba que la IED horitzontal domina als Balcans occidentals, per tant, el creixement de la competitivitat i el desenvolupament econòmic es va intensificar a la regió.

Hollywood Films in the Non-Western World: What Are the Criteria Followed by the Chinese Government When Choosing Hollywood Film Imports?

This dissertation argues that the Government of the People’s Republic of China, when it made the decision to import a quota of Hollywood films in 1994 to revive the failing domestic film industry, had different possible criteria in mind. This project has studied four of them: first, importing films that gave a negative image of the United States; second, importing films that featured Chinese talent or themes; third, importing films that were box office hits in the United States; and fourth, importing films with a strong technological innovation ingredient. In order to find out the most important criteria for the Chinese Government, this dissertation offers a dataset that analyzes a population of 262 Hollywood films released in the PRC between 1994 and 2010. For each unit, a method has been developed to compile data that will determine whether the film reflects any of the four criteria, and findings in the form of yearly percentages have been drawn. Results show that, out of the four studied criteria, the two predominant reasons for China to import films were technological innovation and box office hits. This tells us that, at this point, the Chinese Government shows more interest in obtaining big revenues and learning from technically innovative American films than in delivering underlying political messages. This dissertation contributes to the existing literature by analyzing the content of all the films imported by China between 1994 and 2010, while integrating in the analysis variables based on the existing knowledge.