"Refined mapping of a quantitative trait locus on chromosome 1 responsible for mouse embryonic death"

Recurrent spontaneous abortion (RSA) is defined as the loss of three or more consecutive pregnancies during the first trimester of embryonic intrauterine development. This kind of human infertility is frequent among the general population since it affects 1 to 5% of women. In half of the cases the etiology remains unelucidated. In the present study, we used interspecific recombinant congenic mouse strains (IRCS) in the aim to identify genes responsible for embryonic lethality. Applying a cartographic approach using a genotype/phenotype association, we identified a minimal QTL region, of about 6 Mb on chromosome 1, responsible for a high rate of embryonic death (,30%). Genetic analysis suggests that the observed phenotype is linked to uterine dysfunction. Transcriptomic analysis of the uterine tissue revealed a preferential deregulation of genes of this region compared to the rest of the genome. Some genes from the QTL region are associated with VEGF signaling, mTOR signaling and ubiquitine/proteasome-protein degradation pathways. This work may contribute to elucidate the molecular basis of a multifactorial and complex human disorder as RSA.

Refined mapping of a quantitative trait locus on chromosome 1 responsible for mouse embryonic death

Recurrent spontaneous abortion (RSA) is defined as the loss of three or more consecutive pregnancies during the first trimester of embryonic intrauterine development. This kind of human infertility is frequent among the general population since it affects 1 to 5% of women. In half of the cases the etiology remains unelucidated. In the present study, we used interspecific recombinant congenic mouse strains (IRCS) in the aim to identify genes responsible for embryonic lethality. Applying a cartographic approach using a genotype/phenotype association, we identified a minimal QTL region, of about 6 Mb on chromosome 1, responsible for a high rate of embryonic death (similar to 30%). Genetic analysis suggests that the observed phenotype is linked to uterine dysfunction. Transcriptomic analysis of the uterine tissue revealed a preferential deregulation of genes of this region compared to the rest of the genome. Some genes from the QTL region are associated with VEGF signaling, mTOR signaling and ubiquitine/proteasome-protein degradation pathways. This work may contribute to elucidate the molecular basis of a multifactorial and complex human disorder as RSA.

Pollution solution? : pollution.

Combina historias de ficción y no ficción acerca de la contaminación. De qué manera afecta nuestra vida, cómo agua aire y suelo pueden verse afectadas y cómo se está tratando de resolver los problemas de contaminación tóxica en todo el mundo. Las preguntas abiertas estimulan a los estudiantes a reflexionar sobre estas cuestiones que afectan a las personas en diferentes partes del mundo y a formar sus propias opiniones.

Your life. Student book 4 : the whole-school solution for citizenship and PSHE.

Recurso para la asignatura de la Ciudadanía para alumnos entre once y dieciséis años. Está estructurado en cuatro secciones: las dos primeras se centran en el conocimiento de sí mismo y en la capacidad para manejar las emociones y las relaciones. Las unidades de la sección tres están diseñadas para ayudar a entender cómo desarrollar un estilo de vida saludable, más seguro, a pensar en las alternativas al tomar decisiones sobre sanidad personal y las consecuencias de tales decisiones. En la sección cuatro se trata la comprensión del mundo del trabajo y la capacidad financiera.

Your life KS4 co-ordinator's file : the whole-school solution for citizenship and PSHE.

Libro dirigido a profesores que tengan que impartir la asignatura de 'Educación para la ciudadanía' en relación con 'Educación personal, social y de la salud' en el nivel KS4 (Key Stage 4), enseñanza secundaria. Presenta sugerencias de planificación y materiales adicionales para acompañar a los libros del alumno 'Your life 4' y 'Your life 5'. Cubre los siguientes temas: desarrollo como ciudadano, bienestar personal (comprenderse a si mismo y saber relacionarse), bienestar personal (mantener la salud), bienestar económico y capacidad financiera.

Unlocking Geographical Information from Academia: an Open Source WebGIS Solution

Los Centros de Investigación de Geografía son por lo general productores de un gran volumen de Información Geográfica (IG), los cuales generan tanto proyectos financiados como iniciativas de investigación individuales. El Centro de Estudos de Geografia e Planeamento Regional (e-GEO) ha estado involucrado en varios proyectos a escala local, regional, nacional e internacional. Recientemente, dos cuestiones fueron objeto de debate. Una de ellas fue el hecho de que la información espacial obtenida a partir del desarrollo de tales proyectos de investigación no ha tenido la visibilidad que se esperaba. En la mayoría de las veces, la IG de estos proyectos no estaba en el formato adecuado para que los investigadores -o incluso el público en general o grupos de interés- pudieran pesquisar fácilmente. La segunda cuestión era sobre cómo hacer que estos resultados pudieran ser accesibles al alcance de todos, en todos los lugares, fácilmente y con los mínimos costes para el Centro, teniendo en cuenta el actual contexto económico portugués y los intereses de e-GEO. Estas dos cuestiones se resuelven con una sola respuesta: la puesta en marcha de un WebGIS en una plataforma Open Source. En este trabajo se ilustra la producción de un instrumento para la difusión de las indicaciones geográficas en el World Wide Web, utilizando únicamente software libre y freeware. Esta herramienta permite a todos los investigadores del Centro publicar su IG, la cual aparece como plenamente accesible a cualquier usuario final. Potencialmente, el hecho de permitir que este tipo de información sea plenamente accesible debería generar un gran impacto, acortando las distancias entre el trabajo realizado por los académicos y el usuario final. Creemos que es una óptima manera para que el público pueda acceder e interpretar la información espacial. En conclusión, esta plataforma debería servir para cerrar la brecha entre productores y usuarios de la información geográfica, permitiendo la interacción entre todas las partes así como la carga de nuevos datos dado un conjunto de normas destinadas a control de calidad

A cell by cell anisotropic adaptive mesh ALE scheme for the numerical solution of the Euler equations

In this paper a cell by cell anisotropic adaptive mesh technique is added to an existing staggered mesh Lagrange plus remap finite element ALE code for the solution of the Euler equations. The quadrilateral finite elements may be subdivided isotropically or anisotropically and a hierarchical data structure is employed. An efficient computational method is proposed, which only solves on the finest level of resolution that exists for each part of the domain with disjoint or hanging nodes being used at resolution transitions. The Lagrangian, equipotential mesh relaxation and advection (solution remapping) steps are generalised so that they may be applied on the dynamic mesh. It is shown that for a radial Sod problem and a two-dimensional Riemann problem the anisotropic adaptive mesh method runs over eight times faster.

A cell by cell anisotropic adaptive mesh ALE method

A cell by cell anisotropic adaptive mesh Arbitrary Lagrangian Eulerian (ALE) method for the solution of the Euler equations is described. An efficient approach to equipotential mesh relaxation on anisotropically refined meshes is developed. Results for two test problems are presented.

Consistent Dirichlet Boundary Conditions for Numerical Solution of Moving Boundary Problems

We consider the imposition of Dirichlet boundary conditions in the finite element modelling of moving boundary problems in one and two dimensions for which the total mass is prescribed. A modification of the standard linear finite element test space allows the boundary conditions to be imposed strongly whilst simultaneously conserving a discrete mass. The validity of the technique is assessed for a specific moving mesh finite element method, although the approach is more general. Numerical comparisons are carried out for mass-conserving solutions of the porous medium equation with Dirichlet boundary conditions and for a moving boundary problem with a source term and time-varying mass.

GDASH: a grid-enabled program for structure solution from powder diffraction data

The simulated annealing approach to structure solution from powder diffraction data, as implemented in the DASH program, is easily amenable to parallelization at the individual run level. Very large scale increases in speed of execution can therefore be achieved by distributing individual DASH runs over a network of computers. The GDASH program achieves this by packaging DASH in a form that enables it to run under the Univa UD Grid MP system, which harnesses networks of existing computing resources to perform calculations.

MDASH: a multi-core-enabled program for structure solution from powder diffraction data

The simulated annealing approach to structure solution from powder diffraction data, as implemented in the DASH program, is easily amenable to parallelization at the individual run level. Modest increases in speed of execution can therefore be achieved by executing individual DASH runs on the individual cores of CPUs.

Predicting mesh density for adaptive modelling of the global atmosphere

The shallow water equations are solved using a mesh of polygons on the sphere, which adapts infrequently to the predicted future solution. Infrequent mesh adaptation reduces the cost of adaptation and load-balancing and will thus allow for more accurate mapping on adaptation. We simulate the growth of a barotropically unstable jet adapting the mesh every 12 h. Using an adaptation criterion based largely on the gradient of the vorticity leads to a mesh with around 20 per cent of the cells of a uniform mesh that gives equivalent results. This is a similar proportion to previous studies of the same test case with mesh adaptation every 1–20 min. The prediction of the mesh density involves solving the shallow water equations on a coarse mesh in advance of the locally refined mesh in order to estimate where features requiring higher resolution will grow, decay or move to. The adaptation criterion consists of two parts: that resolved on the coarse mesh, and that which is not resolved and so is passively advected on the coarse mesh. This combination leads to a balance between resolving features controlled by the large-scale dynamics and maintaining fine-scale features.

The influence of mineral solubility and soil solution concentration on the toxicity of copper to Eisenia fetida Savigny

The OECD 14 d earthworm acute toxicity test was used to determine the toxicity of copper added as copper nitrate (Cu(NO3)(2)), copper sulphate (CuSO4) and malachite (Cu-2(OH)(2)(CO3)) to Eisenia fetida Savigny. Cu(NO3)(2), and CuSO4 were applied in both an aqueous (aq) and solid (s) form, Cu-2(OH)(2)(CO3) was added as a solid. Soil solution was extracted by centrifugation, and analysed for copper. Two extractants [0.01 M CaCl2 and 0.005 M diethylenetriminpentaacetic acid (DTPA)] were used as a proxy of the bioavailable copper fraction in the soil. For bulk soil copper content the calculated copper toxicity decreased in the order nitrate > sulphide > carbonate, the same order as decreasing solubility of the metal compounds. For Cu(NO3)(2) and CuSO4, the LC50s obtained were not significantly different when the compound was added in solution or solid form. There was a significant correlation between the soil solution copper concentration and the percentage earthworm mortality for all 3 copper compounds (P less than or equal to 0.05) indicating that the soil pore water copper concentration is important for determining copper availability and toxicity to E. fetida. In soil avoidance tests the earthworms avoided the soils treated with Cu(NO3)(2) (aq and s) and CuSO4 (aq and s), at all concentrations used (110-8750 mug Cu g(-1), and 600-8750 mug Cu g(-1) respectively). In soils treated with Cu-2(OH2)CO3, avoidance behaviour was exhibited at all concentrations greater than or equal to3500 mug Cu g(-1). There was no significant correlation between the copper extracted by either CaCl2 or DTPA and percentage mortality. These two extractants are therefore not useful indicators of copper availability and toxicity to E. fetida.