Softening-up mannan-rich cell walls

The softening and degradation of the cell wall (CW), often mannan enriched, is involved in several processes during development of higher plants, such as meristematic growth, fruit ripening, programmed cell death, and endosperm rupture upon germination. Mannans are also the predominant hemicellulosic CW polymers in many genera of green algae. The endosperm CWs of dry seeds often contain mannan polymers, sometimes in the form of galactomannans (Gal-mannans). The endo-beta-mannanases (MANs) that catalyse the random hydrolysis of the beta-linkage in the mannan backbone are one of the main hydrolytic enzymes involved in the loosening and remodelling of CWs. In germinating seeds, the softening of the endosperm seed CWs facilitates the emergence of the elongating radicle. Hydrolysis and mobilization of endosperm Gal-mannans by MANs also provides a source of nutrients for early seedling growth, since Gal-mannan, besides its structural role, serves as a storage polysaccharide. Therefore, the role of mannans and of their hydrolytic enzymes is decisive in the life cycle of seeds. This review updates and discusses the significance of mannans and MANs in seeds and explores the increasing biotechnological potential of MAN enzymes.

Failure mechanism of reinforced concrete structural walls with and without confinement

This paper presents the results of cyclic loading tests on two large-scale reinforced concrete structural walls that were conducted at Purdue University. One of the walls had confinement reinforcement meeting ACI-318-11 requirements while the other wall did not have any confinement reinforcement. The walls were tested as part of a larger study aimed at indentifying parameters affecting failure modes observed to limit the drift capacity of structural walls in Chile during the Maule Earthquake of 2010. These failure modes include out-of-plane buckling (of the wall rather tan individual reinforcing bars), compression failure, and bond failure. This paper discusses the effects of confinement on failure mode. Distributions of unit strain and curvature obtained with a dense array of non-contact coordinate-tracking targets are also presented.

Rammed earth walls in the late middle age castles in the actual province of Soria, Spain

This paper analyzed the building techniques based in the use of rammed earth in the Late Medieval fortifications in the province of Soria, Spain. The manorial castles of Serón de Nágima and Yanguas were built completely with rammed earth. However, these techinques are different. Through the study of the constructive signals, we can reconstruct the constructive process and made an hypothesis of the auxiliary scaffolding necessary for the construction. After, other four cases in which there is presence of rammed earth are described: Ágreda, Arcos de Jalón, Caracena and the tower of Martín González or castle of La Raya (the Border). Rammed earth is used in some secondary architectural elements and also as a filling of the masonry walls, but somtimes there are ancient rammed earth walls overlaid with stone masonry.

On the use of trenches and walls on the control of ground transmitted railway vibrations

An actual case of an underground railway in the neighbourhood of habitation buildings has been analyzed. The study has been based on a twodimensional BEM model including a tunnel and a typical building. The soil properties were obtained using geophysical techniques. After a sensitivity study, the model has been simplyfied and validated by comparison with "in situ" measurements. Using this simplyfied model, a parametric study has been done including trenches and walls of different materials and different depths at two different distances from the tunnel. The reductions obtained with the different solutions can then be compared.

Performance of buildings with masonry infill walls during the 2011 Lorca earthquake

On Wednesday 11th May 2011 at 6:47 pm (local time) a magnitude 5.1 Mw earthquake occurred 6 km northeast of Lorca with a depth of around 5 km. As a consequence of the shallow depth and the small epicentral distance, important damage was produced in several masonry constructions and even led to the collapse of one of them. Pieces of the facades of several buildings fell down onto the sidewalk, being one of the reasons for the killing of a total of 9 people. The objective of this paper is to describe and analyze the failure patterns observed in reinforced concrete frame buildings with masonry infill walls ranging from 3 to 8 floors in height. Structural as well as non-structural masonry walls suffered important damage that led to redistributions of forces causing in some cases the failure of columns. The importance of the interaction between the structural frames and the infill panels is analyzed by means of non-linear Finite Element Models. The resulting load levels are compared with the member capacities and the changes of the mechanical properties during the seismic event are described and discussed. In the light of the results obtained the observed failure patterns are explained. Some comments are stated concerning the adequacy of the numerical models that are usually used during the design phase for the seismic analysis.

Seismic earth thrust against massive building walls

The problem of earth thrust for a massive building in a seismic environment is analysed. Intertial and kinematic soil-structure interactions are considered by modelling the soil and the structure together. The problem is solved in the frequency domain by using the computer code FLUSH. Results show that the horizontal component of the seismic earth thrust is much greater than that obtained when applying the Mononobe-Okabe theory. This study establishes a set of conclusicns and recommendations for both design and practical purposes, and proposes future lines of investigation.

dOTM: a mechanism for distributing centralized multi-party video conferencing in the cloud

One of the key factors for a given application to take advantage of cloud computing is the ability to scale in an efficient, fast and reliable way. In centralized multi-party video conferencing, dynamically scaling a running conversation is a complex problem. In this paper we propose a methodology to divide the Multipoint Control Unit (the video conferencing server) into more simple units, broadcasters. Each broadcaster receives the media from a participant, processes it and forwards it to the rest. These broadcasters can be distributed among a group of CPUs. By using this methodology, video conferencing systems can scale in a more granular way, improving the deployment.

Selective injection of magnetic domain walls in Permalloy nanostripes

Selective injection of magnetic domain walls in Permalloy nanostripes

Pulsatile blood flow interaction with arterial walls of aorta : autoregulation and impedance pressure boundary condition and its biomedical applications

Para las decisiones urgentes sobre intervenciones quirúrgicas en el sistema cardiovascular se necesitan simulaciones computacionales con resultados fiables y que consuman un tiempo de cálculo razonable. Durante años los investigadores han trabajado en diversos métodos numéricos de cálculo que resulten atractivos para los cirujanos. Estos métodos, precisos pero costosos desde el punto de vista del coste computacional, crean un desajuste entre la oferta de los ingenieros que realizan las simulaciones y los médicos que operan en el quirófano. Por otra parte, los métodos de cálculo más simplificados reducen el tiempo de cálculo pero pueden proporcionar resultados no realistas. El objetivo de esta tesis es combinar los conceptos de autorregulación e impedancia del sistema circulatorio, la interacción flujo sanguíneo-pared arterial y modelos geométricos idealizados tridimensionales de las arterias pero sin pérdida de realismo, con objeto de proponer una metodología de simulación que proporcione resultados correctos y completos, con tiempos de cálculo moderados. En las simulaciones numéricas, las condiciones de contorno basadas en historias de presión presentan inconvenientes por ser difícil conocerlas con detalle, y porque los resultados son muy sensibles ante pequeñas variaciones de dichas historias. La metodología propuesta se basa en los conceptos de autorregulación, para imponer la demanda de flujo aguas abajo del modelo en el ciclo cardiaco, y la impedancia, para representar el efecto que ejerce el flujo en el resto del sistema circulatorio sobre las arterias modeladas. De este modo las historias de presión en el contorno son resultados del cálculo, que se obtienen de manera iterativa. El método propuesto se aplica en una geometría idealizada del arco aórtico sin patologías y en otra geometría correspondiente a una disección Stanford de tipo A, considerando la interacción del flujo pulsátil con las paredes arteriales. El efecto de los tejidos circundantes también se incorpora en los modelos. También se hacen aplicaciones considerando la interacción en una geometría especifica de un paciente anciano que proviene de una tomografía computarizada. Finalmente se analiza una disección Stanford tipo B con tres modelos que incluyen la fenestración del saco. Clinicians demand fast and reliable numerical results of cardiovascular biomechanic simulations for their urgent pre-surgery decissions. Researchers during many years have work on different numerical methods in order to attract the clinicians' confidence to their colorful contours. Though precise but expensive and time-consuming methodologies create a gap between numerical biomechanics and hospital personnel. On the other hand, simulation simplifications with the aim of reduction in computational time may cause in production of unrealistic outcomes. The main objective of the current investigation is to combine ideas such as autoregulation, impedance, fluid-solid interaction and idealized geometries in order to propose a computationally cheap methodology without excessive or unrealistic simplifications. The pressure boundary conditions are critical and polemic in numerical simulations of cardiovascular system, in which a specific arterial site is of interest and the rest of the netwrok is neglected but represented by a boundary condition. The proposed methodology is a pressure boundary condition which takes advantage of numerical simplicity of application of an imposed pressure boundary condition on outlets, while it includes more sophisticated concepts such as autoregulation and impedance to gain more realistic results. Incorporation of autoregulation and impedance converts the pressure boundary conditions to an active and dynamic boundary conditions, receiving feedback from the results during the numerical calculations and comparing them with the physiological requirements. On the other hand, the impedance boundary condition defines the shapes of the pressure history curves applied at outlets. The applications of the proposed method are seen on idealized geometry of the healthy arotic arch as well as idealized Stanford type A dissection, considering the interaction of the arterial walls with the pulsatile blood flow. The effect of surrounding tissues is incorporated and studied in the models. The simulations continue with FSI analysis of a patient-specific CT scanned geometry of an old individual. Finally, inspiring of the statistic results of mortality rates in Stanford type B dissection, three models of fenestrated dissection sac is studied and discussed. Applying the developed boundary condition, an alternative hypothesis is proposed by the author with respect to the decrease in mortality rates in patients with fenestrations.

Mapping strain exerted on blood vessel walls using deuterium double-quantum-filtered MRI

A technique is described for displaying distinct tissue layers of large blood vessel walls as well as measuring their mechanical strain. The technique is based on deuterium double-quantum-filtered (DQF) spectroscopic imaging. The effectiveness of the double-quantum filtration in suppressing the signal of bulk water is demonstrated on a phantom consisting of rat tail tendon fibers. Only intrafibrillar water is displayed, excluding all other signals of water molecules that reorient isotropically. One- and two-dimensional spectroscopic imaging of bovine aorta and coronary arteries show the characteristic DQF spectrum of each of the tissue layers. This property is used to obtain separate images of the outer layer, the tunica adventitia, or the intermediate layer, the tunica media, or both. To visualize the effect of elongation, the average residual quadrupole splitting <Δνq> is calculated for each pixel. Two-dimensional deuterium quadrupolar splitting images are obtained for a fully relaxed and a 55% elongated sample of bovine coronary artery. These images indicate that the strong effect of strain is associated with water molecules in the tunica adventitia whereas the DQF NMR signal of water in the tunica media is apparently strain-insensitive. After appropriate calibration, these average quadrupolar splitting images can be interpreted as strain maps.

The Involvement of Hydrogen Peroxide in the Differentiation of Secondary Walls in Cotton Fibers1

H2O2 is a widespread molecule in many biological systems. It is created enzymatically in living cells during various oxidation reactions and by leakage of electrons from the electron transport chains. Depending on the concentration H2O2 can induce cell protective responses, programmed cell death, or necrosis. Here we provide evidence that H2O2 may function as a developmental signal in the differentiation of secondary walls in cotton (Gossypium hirsutum) fibers. Three lines of evidence support this conclusion: (a) the period of H2O2 generation coincided with the onset of secondary wall deposition, (b) inhibition of H2O2 production or scavenging the available H2O2 from the system prevented the wall differentiation process, and (c) exogenous addition of H2O2 prematurely promoted secondary wall formation in young fibers. Furthermore, we provide support for the concept that H2O2 generation could be mediated by the expression of the small GTPase Rac, the accumulation of which was shown previously to be strongly induced during the onset of secondary wall differentiation. In support of Rac's role in the activation of NADPH oxidase and the generation of reactive oxygen species, we transformed soybean (Glycine max) and Arabidopsis cells with mutated Rac genes. Transformation with a dominantly activated cotton Rac13 gene resulted in constitutively higher levels of H2O2, whereas transformation with the antisense and especially with dominant-negative Rac constructs decreased the levels of H2O2.