Condição imunológica de bovinos das raças Holandesa e Nelore frente a Babesia bovis e B. bigemina em duas regiões do Estado de São Paulo

The immunological reply of a population to an infectious agent can vary between races and handling of this population. Regional research becomes important, in order to know the interrelation between the agent and its host. In this way, the occurrence of immunoglobulins of class G, anti-Babesia bovis and anti-Babesia bigemina in the Nelore (Bos indicus) and Hostein breed (Bos taurus), was investigated in two regions of the State of São Paulo, 300 km distant from each other. For the indirect method of ELISA, 1, 161 bovine serum samples were tested. The medium frequencies of antibodies showed that in the two regions exists an enzootic stability for B. bovis in both breeds studied; even so there was a tendency of marginal area for the Nelore breed in one of the regions. Regarding B. bigemina, in both regions exists enzootic stability for the Hostein and enzootic instability for the Nelore breed. Therefore, acute cases of the disease or specific outbreaks by B. bigemina infection in the Nelore breed may occur in these regions.

Seleção de extratores químicos para avaliação da disponibilidade de zinco em solos do estado de São Paulo

The purpose of this study was to evaluate methods for predicting availability of Zn in soils. Surface soil samples representing Podzolized soils from Lins and Marilia var. Marilia and var. Lins, medium texture Dark-Red Latosol, Dusky-Red Latosol and Terra Roxa Estruturada, were gathered from fields in Jaboticabal, SP, Brazil. Com was used as indicator plant in a greenhouse experiment. Availability of Zn was determined with: 0.5 M CaCl2, 2.0 N MgCl2, N CH3COONH4 (pH 6.0 and 4.8), 0.05 and 0.1 N HCl, 0.05 N H2SO4, 0.005 M DTPA (pH 7.3 and 6.0), 1.0% Na2-EDTA, 0.01 M EDTA + 1.0 M (NH4)2CO3 (pH 8.6) and 0.02 M EDTA + 0.5 M CH3COOH + 0.5 M CH3COONH4. By using the simple correlation coefficients (Zn extracted from soils x absorbed by plants it can be concluded that the complexant agents and acid solutions showed to be efficient. The salt solutions showed low extraction capacity, low correlation coefficients and less accuracy. By considering aspects as accuracy and easy management DTPA pH 7,3 and Na2-EDTA can be indicated as the most apropriates (r values, respectively, 0.873** and 0.868** and V.C. 7.52% and 7.98%).

Variabilidade da precipitação pluvial na UGRH do Médio Paranapanema, Estado de São Paulo

The purpose of this work was to analyze the variability of pluvial precipitation at the Mid-Paranapanema Hydric Resources Management Unit (UGRI II-17), both spatially and temporally. To that effect, 33 pluviometric series were used, obtained from the National Water Agency (ANA), for the period from 1940 to 2000. The averages, standard deviation, upper and lower quartiles, maximum rainfalls, within the analyzed period, as well as the anomalies in these series, were calculated. The anomalies of the 1982/1983 El Nino, as well as the classification of homogeneous areas inside the basin were also calculated. Variability was observed from one year to the other, with rainier years - for example, 1982 and 1983 - and drier years - such as 1985 and 2000.

Biologia populacional de Pareiorhina rudolphi (Loricariidae, Hypostominae) na microbacia do Ribeirão Grande, serra da Mantiqueira oriental, Estado de São Paulo

Pareiorhina rudolphi was sampled in streams of the Ribeirao Grande system, eastern Serra da Mantiqueira (22[degree]47[minute]08[second]S, 45[degree]28[minute]17[second] W). Samplings were carried out using an electrofishing device, during the months of July/2001, October/2001, February/2002 and April/2002. Sex-ratio diverged significantly from the expected 1: 1 ratio([chi]2 = 6.53; p < 0.05), standing at 1.6:1 (female: male). The spawning period for Pareiorhina rudolphi lasts from spring to summer, with, the highest observed, in October and February by the gonadosomantic index and the relative condition factor coincided with the spawning period. The length at sexual maturity of P. rudolphi is about 4.45 cm for both sexes. The absolute fecundity was low, and ranged from 4 to 11 oocytes. The periphyton was used as a direct food source by the species, which remain attached to the substrate with their large circular lips, and use their conspicuous Slightly Yellowish teeth to graze the periphyton. The growth parameters, natural mortality rate and survival rate for P, rudolphi were respectively: K = 0.35 year-1, L[infinity] = 7.2 cm, tmax = 8.6 years, M = 1.1 year-1, S = 33%. The characteristics presented by P. rudolphi occur in the environment function of a population adjustment, and not of species abundance.

Aspectos da reprodução e alimentação de Neoplecostomus microps (Loricariidae, Neoplecostominae) na microbacia do Ribeirão Grande, serra da Mantiqueira oriental (Estado de São Paulo)

Reproduction and feeding aspects of Neoplecostomus microps (Loricariidae, Neoplecostominae) in the Ribeirao Grande system, eastern Serra da Mantiqueira (São Paulo State). The Ribeirao Grande system is located in the slope of the Serra da Mantiqueira and is surrounded by Atlantic forest. Eight sites were surveyed in the Ribeirao Grande system, during the winter (July 2001), spring (October 2001), summer (February 2002) and fall (April 2002). Samples were made with an electrofishing device. Gonad maturation, levels of stomach fullness and fat content were analyzed and their distributions reported in an annual cycle. Neoplecostomus microps has a spawning in the spring through to summer. The size at sexual maturity is about 5.9 cm total length for males and 6.9 cm for females. The high values in October and February by the gonadosomatic index and the relative condition factor coincided with the spawning period. In the diet of N. microps were found Diptera larvae (Simulium, Chironomus), Plecoptera nymphs and Coleoptera aquatic Larvae (Psephenus). The increased feeding from summer to fall provided fat accumulation. During subsequent seasons, fish may utilize visceral fat reserves for maintenance and reproduction. The reproductive pattern and feeding are interpreted as being an adaptation with respect to temporal and spatial variation and food availability.

Biologia populacional de Imparfinis minutus (Siluriformes, Heptapteridae) na microbacia do Ribeirão Grande, serra da Mantiqueira oriental, Estado de São Paulo

Populational biology of Imparfinis minutus (Siluriformes, Heptapteridae) in the Ribeirao Grande microbasin, eastern Mantiqueira mountain range, São Paulo State. The aim of the work was to analyze biological aspects and distribution of Imparfinis minutus in the Ribeirao Grande microbasin (22[degree]4708[minute]''S, 45[degree]28[minute]17''W), in eastern Mantiqueira mountain range, from July 2001 to April 2002. Among the 37 species sampled, I. minutus (Siluriformes, Heptapteridae), was distributed from the piedmont to the plains of the Vale do Paraiba. The occurrence of this species was associated with the environments of small water volume, bottom with stones and gravel. The amplitude length of I. minutus was 4.75 to 12.75 cm. The length-weight relationship of the population was estimated at W = 0.0052 L3,13. Analyzing the alometric (Ka) and relative (Kr) condition factors, both indexes presented similar values. The lowest value was obtained in July, increasing gradually in October and January, and peaking in April. The analysis of the gonadossomatic relationship (GSR) of females showed higher values in July and January. The reproductive period of I. minutus occurred on spring/summer (October to January), as suggested by the gonadossomatic relationship and condition factor results. Feeding activity was constant, with fat visceral deposition concentrated in April and July. Imparfinis minutus is an aquatic insectivore opportunist species, consuming Diptera and Trichoptera larvae as secondary items. Most ingested food items were autochthonous resources.

Ephemeroptera, Plecoptera e Trichoptera em corredeiras de riachos do Parque Estadual Intervales, Estado de São Paulo.

The fauna of Ephemeroptera, Plecoptera and Trichoptera (EPT) from Intervales State Park has been recorded since the 1990s. As a result of these sampling efforts, we present in this study a list of EPT genera in riffles from streams of Intervales State Park, São Paulo State.

Expansao das lavouras de soja e trigo no Estado de São Paulo

Shows the viability of using the Trend Surface Analysis (TSA) in studies of the distribution of items of geographical interest in time and space; tests comparatively the techniques of cartographic representation conventionally used in TSA. It follows that the TSA offers more resources for analyses of the spatial items behaviour than the techniques commonly employed for the same purpose.- English summary

Anemia em crianças menores de dois anos atendidas nas unidades básicas de saúde no Estado de São Paulo, Brasil

The present study sought to determine the prevalence of anemia in 2,992 children, aged between 6 and 23 months, who voluntarily attended 160 Basis Health Care Units, located in 63 cities of the 5 Regional Health Coordinating areas of the State of S. Paulo, Brazil. Blood samples were collected by venous puncture and hemoglobin was measured by the cianometahemoglobin method. The WHO criteria for the diagnosis of anemia (Hb < 11.0 g/dl) were used: 59.1% of the children were shown to be anemic, with prevalence varying from 47.8% to 68.7% in the 5 RHCs. RHC 1, which comprises the Greater S. Paulo Region, presented a prevalence of anemia significantly lower than the other 4 RHCs, which cover the rest of the State. Hemoglobin levels 9.5 g/dl were found in 25.1% of the children. Anemia was more frequent in male children in male children, in those born, with a weight of less than 3,000 gr, in those who were breastfed for less than 2 months and in those that who presented some degree of energy deficient proteic malnutrition, according to Gomez's criteria. This is the first of a series of 4 articles whose purpose is the determining the prevalence of anemia in the State of S. Paulo and of testing the intervention alternatives with a view to curtailing the incidence of this pathology which today is the most prevalent nutritional disturbance in the world.

Uso de macroalgas para avaliação da Poluição orgânica no Rio Preto, noroeste do estado de São Paulo.

The Preto River, located in the northwest of São Paulo State, receives a total wastewater load of 15.150 kg DBO day-1, from which 13.685 kg DBO day-1 (90.5%) corresponds to domestic sewage, and the city of São José do Rio Preto contributes with 12.400 kg DBO day-1 (90% of domestic sewage). During the period from August 1990 through January 1991, monthly sampling was carried out to evaluate the use of macroalgae as bioindicator of organic pollution. Five sampling sites were established along the main river and the following variables were analised: temperature, conductance, turbidity, dissolved oxygen, BOD, COD, total and fecal coliforms, and composition and abundance of macroalgal communities. Data were submitted to analysis of variance, correlation coefficient, cluster analysis (four different approaches) and converted to biological indices (species deficit, relative pollution, saprobity, diversity and uniformity indices). A wide range in water quality was found (particularly for conductance, oxygen, BOD and COD) among the sampling sites, which were classified into three groups (polluted, moderately polluted and unpolluted/weakly polluted). As regards the occurrence and abundance of macroalgae the Rhodophyta were found only in unpolluted or weakly polluted sites, whereas Cyanophyta occurred mostly under high pollution load; the Chlorophyta species were observed under a wide range of conditions. Among the biological indices, saprobity was the most sensitive and correlated to all water variables and the other indices. Cluster analyses showed that the composition of macroalgal communities was consistent with the levels of organic pollution in the Preto River.

Epidemiologia e quadro clínico do botulismo epizoótico dos bovinos no Estado de São Paulo

In order to investigate epidemiological and clinical aspects of beef cattle mortalities caused by botulism, a syndrome popularly known as doença da vaca caída, studies were carried out in 32 naturally affected 4 to 9 year old cows, 27 belonging to the Nellore breed and 5 to crossbred Nellore, all from 27 farms located in municipalities near Botucatu, State of São Paulo. The epidemiological and clinical features were based, respectively, on the farm and herd managements, and on the general physical examination of the cows. Mouse bioassay and complement microfixation tests were performed to detect the presence of botulinum toxins in liver samples. The results showed that the disease occurs in beef cattle of range breeding systems, reared under inadequate mineral nutrition and deficient health management. Pregnant and milking cows represented the group at risk, and the incidence was higher during the rainy season (December to March), with morbidity and mortality rates of 3,2 ± 3,6%. Clinical examination revealed cows with no alterations of vital signs, behavior, visual and auditory acuities and skin sensation; but revealed ruminal hypomotility, anorexia, dehydration, flaccid para or tetraparesis with permanent recumbency, and a paretic or paralytic tongue. The diagnosis of botulism, involving type C and D toxins, was consistent with the epidemiological and clinical findings.

Pesquisa de anticorpos contra Brucella ovis em ovinos do Estado de São Paulo

Eight hundred and fifty sheep sera from 18 herds, located in 15 municipalities of the State of São Paulo, were examined by complement fixation test, indirect enzyme immunoassay and gel diffusion test for antibodies to Brucella ovis. The combination of the serological test results and the clinical and epidemiological data suggest that none of the examined animals was infected. The complement fixation and the gel diffusion tests showed good specificity, but the indirect enzyme immunoassay showed false positive results in nine of the sera.

Argilominerais de Coberturas Sedimentares Cenozóicas do Centro-leste do Estado de São Paulo

Clay mineral analysis contributed to the mineralogical characterization and stratigraphic setting of continental Cenozoic deposits of central-eastern portion of São Paulo State, southeastern Brazil. These deposits, often thin and discontinuous, comprise the Itaqueri Formation (Cretaceous-Paleogene), Rio Claro Formation (Pliocene) and associated Neocenozoic deposits. X-ray diffractometry provided mineralogical and semiquantitative constitution of clays, while scanning electron microscopy (SEM) provided details about mineralogical constitution, packing of microscopic grains and nature (detrital or diagenetic) of clays. These results supported interpretations about paleoenvironmental conditions during sedimentation, revealing possible links between changing climates (dry or humid) and correlative Cenozoic deposits in southeastern Brazil. In addition, SEM images showed microstructures which influence on geotechnical behaviour (erosion and collapse phenomena) of sedimentary materials.

Adubação verde em Citrus: Influência na porosidade e retenção de água de um latossolo vermelho-escuro do noroeste do estado de São Paulo

The objective of this research was to study the porosity, bulk density and retention of water of an Oxisol, located in the Northwestern region of Sn̄o Paulo state, Brazil. The soil was cultivated with Citrus sp., to which green manure was applied between rows for three years. Each of six species of green manure crops (Crotalaria juncea L., Mucuna deeringiana Steph. & Bart., Canavalia ensiformis L. DC., Cajanus cajan L., Lablab purpureum L. and Ricinus communis L.) were seeded for three years (1995, 1996 and 1997) between Citrus rows, plus a treatment with a mix of all six species and a control (natural regrowth af vegetation). The experimental design was a randomized complete block design, with four replications for each of the eight treatments. Water retention, microporosity, macroporosity, total porosity and bulk density were analyzed in the beginning (1995) and end (1997) of the experiment, at three depth ranges (0-0.10; 0.10-0.20 and 0.20-0.40m). We concluded that there were statistically significant differences for bulk density, macroporosity, total porosity and retention of water among the different soil depth ranges; there were no significant differences among treatments though.

Modelagem gravimétrica da Bacia de Taubaté-vale do Rio Paraíba do Sul, leste do estado de São Paulo

This paper presents the gravity survey refinement of the Paraíba do Sul river valley, east region of São Paulo state, by acquisition of 646 gravity stations. Further than the traditional Free-Air and Bouguer corrections, it was necessary to apply the Terrain Correction due the proximity of the Mantiqueira and Mar mountain ranges. An enhanced understanding of Taubaté Basin structural framework was the main objetive of this work. As result, we present the Bouguer Gravity Map of the east region of São Paulo State and the Residual Bouguer Gravity Map generated by Upward Continuation and High-pass filtering. The 2D Gravity Modeling was applied in 4 sections, using bi-dimensional models for the bodies that produced the anomalies (Talwani Method). After integrating the gravity data with the interpretation of 11 seismic sections, it was possible to delineate the Taubate Basin structural framework, which shows an utmost sedimentary thickness of 800 m in the depocenters. It was also interpreted an ultramaphic alkaline plug in the subsurface of Caçapava city.