Protein kinase CK2 interacts with and phosphorylates the Arabidopsis circadian clock-associated 1 protein

The circadian clock-associated 1 (CCA1) gene encodes a Myb-related transcription factor that has been shown to be involved in the phytochrome regulation of Lhcb1*3 gene expression and in the function of the circadian oscillator in Arabidopsis thaliana. By using a yeast interaction screen to identify proteins that interact with CCA1, we have isolated a cDNA clone encoding a regulatory (β) subunit of the protein kinase CK2 and have designated it as CKB3. CKB3 is the only reported example of a third β-subunit of CK2 found in any organism. CKB3 interacts specifically with CCA1 both in a yeast two-hybrid system and in an in vitro interaction assay. Other subunits of CK2 also show an interaction with CCA1 in vitro. CK2 β-subunits stimulate binding of CCA1 to the CCA1 binding site on the Lhcb1*3 gene promoter, and recombinant CK2 is able to phosphorylate CCA1 in vitro. Furthermore, Arabidopsis plant extracts contain a CK2-like activity that affects the formation of a DNA–protein complex containing CCA1. These results suggest that CK2 can modulate CCA1 activity both by direct interaction and by phosphorylation of the CCA1 protein and that CK2 may play a role in the function of CCA1 in vivo.

Phytochrome B and the Regulation of Circadian Ethylene Production in Sorghum1

The sorghum (Sorghum bicolor L. Moench) cultivar 58M, which contains the null mutant phytochrome B gene, shows reduced photoperiodic sensitivity and exhibits a shade-avoidance phenotype. Ethylene production by seedlings of wild-type and phytochrome B mutant cultivars was monitored every 3 h, and both cultivars were found to produce ethylene in a circadian rhythm, with peak production occurring during the day. The phytochrome B mutant produces rhythmic peaks of ethylene with approximately 10 times the amplitude of the wild-type counterpart with the same period and diurnal timing. The source of the mutant's additional ethylene is the shoot. The diurnal rhythm can be produced with either light or temperature cycles; however, both light and temperature cycles are required for circadian entrainment. The temperature signal overrides the light signal in the production of diurnal rhythms, because seedlings grown under thermoperiods reversed with the photoperiod produced ethylene peaks during the warm nights. To examine the effect of extreme shading on ethylene production, seedlings were grown under dim, far-red-enriched light. This treatment duplicated the phytochrome B mutant's shade-avoidance phenotype in the wild type and caused the wild type to produce ethylene peaks similar to those observed in the mutant. The results confirm that phytochrome B is not required for proper function of circadian timing, but it may be involved in modulating physiological rhythms driven by the biological clock oscillator.

Light-induced phase-delay of the chicken pineal circadian clock is associated with the induction of cE4bp4, a potential transcriptional repressor of cPer2 gene

The chicken pineal gland contains the autonomous circadian oscillator together with the photic-input pathway. We searched for chicken pineal genes that are induced by light in a time-of-day-dependent manner, and isolated chicken homolog of bZIP transcription factor E4bp4 (cE4bp4) showing high similarity to vrille, one of the Drosophila clock genes. cE4bp4 was expressed rhythmically in the pineal gland with a peak at very early (subjective) night under both 12-h light/12-h dark cycle and constant dark conditions, and the phase was nearly opposite to the expression rhythm of cPer2, a chicken pineal clock gene. Luciferase reporter gene assays showed that cE4BP4 represses cPer2 promoter through a E4BP4-recognition sequence present in the 5′-flanking region, indicating that cE4BP4 can down-regulate the chick pineal cPer2 expression. In vivo light-perturbation studies showed that the prolongation of the light period to early subjective night maintained the high level expression of the pineal cE4bp4, and presumably as a consequence delayed the onset of the induction of the pineal cPer2 expression in the next morning. These light-dependent changes in the mRNA levels of the pineal cE4bp4 and cPer2 were followed by a phase-delay of the subsequent cycles of cE4bp4/cPer2 expression, suggesting that cE4BP4 plays an important role in the phase-delaying process as a light-dependent suppressor of cPer2 gene.

Circadian gating of cell division in cyanobacteria growing with average doubling times of less than 24 hours.

To ascertain whether the circadian oscillator in the prokaryotic cyanobacterium Synechococcus PCC 7942 regulates the timing of cell division in rapidly growing cultures, we measured the rate of cell division, DNA content, cell size, and gene expression (monitored by luminescence of the PpsbAI::luxAB reporter) in cultures that were continuously diluted to maintain an approximately equal cell density. We found that populations dividing at rates as rapid as once per 10 h manifest circadian gating of cell division, since phases in which cell division slows or stops recur with a circadian periodicity. The data clearly show that Synechococcus cells growing with doubling times that are considerably faster than once per 24 h nonetheless express robust circadian rhythms of cell division and gene expression. Apparently Synechococcus cells are able to simultaneously sustain two timing circuits that express significantly different periods.

Instrumentação para a caracterização dielétrica de filmes biodegradáveis

A caracterização dielétrica de um material pode ser usada como uma técnica não destrutiva para avaliar e monitorar sua qualidade, bem como no entendimento da relação estrutura-propriedade de um material, através de suas propriedades dielétricas em função da frequência, temperatura, composição química do material, dentre outros. Na literatura há escassez de trabalhos e dados de caracterização dielétrica de filmes a base de biopolímeros. Diante desse contexto, o objetivo deste trabalho foi o desenvolvimento e a construção de uma instrumentação alternativa a equipamentos disponíveis no mercado, como analisadores de rede e de impedância, que pudesse ser utilizada para a caracterização dielétrica de filmes biodegradáveis a base de gelatina. Foi utilizado o método de placas paralelas na determinação da parte real da permissividade conhecida como permissividade relativa ou constante dielétrica (ε\'). O circuito utilizado para a instrumentação foi um oscilador astável com funcionamento baseado no amplificador operacional (741) chaveado pela carga de um capacitor de placas paralelas cujo dielétrico foi uma amostra de filme biodegradável. A partir dos valores da frequência de oscilação e geometria do capacitor, foi possível calcular a capacitância de cada amostra e, consequentemente obter os valores da permissividade relativa do filme, usando relações básicas bem estabelecidas. Os filmes de gelatina foram produzidos pela técnica de casting sendo utilizados como plastificantes o glicerol (G), o sorbitol (S) e suas misturas, na proporção (G:S) de 30:70, 50:50 e 70:30. Os filmes foram caracterizados quanto à umidade e cristalinidade. A permissividade relativa (ε\') dos filmes, determinada a temperatura ambiente, foi avaliada em função da frequência (5 a 50 kHz), tempo de armazenamento, do teor de umidade e tipo de plastificante. A instrumentação projetada e construída foi capaz de medir com precisão a permissividade relativa das amostras, sendo que essa propriedade diminuiu com o aumento da frequência para todos os filmes. Mantendo-se a frequência constante, não houve variação de ε\' para os filmes de gelatina, independente do plastificante, ao longo de um mês de armazenamento a 24 ± 3 °C. O efeito da umidade foi observado em frequências menores que 25 kHz, sendo que quanto maior o teor de umidade maior a permissividade relativa. O efeito do tipo de plastificante na permissividade relativa dos filmes foi observado a baixas frequências (5 kHz) e filmes plastificados com sorbitol apresentaram maiores valores de ε\'. Os filmes plastificados com maior teor de umidade apresentaram menor cristalinidade, portanto maior mobilidade molecular e consequentemente maior a permissividade relativa.

Age determination and stable isotope ratios of planktonic foraminifera from DSDP Hole 94-609

As shown by the work of Dansgaard and his colleagues, climate oscillations of one or so millennia duration punctuate much of glacial section of the Greenland ice cores. These oscillations are characterized by 5°C air temperature changes, severalfold dust content changes and 50 ppm CO2 changes. Both the temperature and CO2 change are best explained by changes in the mode of operation of the ocean. In this paper we provide evidence which suggests that oscillations in surface water conditions of similar duration are present in the record from a deep sea core at 50°N. Based on this finding, we suggest that the Greenland climate changes are driven by oscillations in the salinity of the Atlantic Ocean which modulate the strength of the Atlantic's conveyor circulation.

Scientific report : RF Project 464 /

1. The determination and interpretation of electronic collision cross sections -- 2. An electron spectrometer for the study of inelastic collision cross sections -- 3. The inelastic scattering of electrons by helium -- 4. Inelastic collision cross sections of carbon monoxide -- 5. An electron impact study of nitrogen in the kinetic energy range 400 to 600 volts -- 6. Electronic collision cross sections and oscillator strengths for oxygen in the Schumann-Runge region -- 7. Electronic collision cross sections for oxygen at excitation energies above 10 volts -- 8. Electronic collsion cross sections for nitrogen at excitation energies from 10 to 80 electron volts -- 9. Additional collision cross sections for helium, especially in the ionized continuum -- 10. A collision cross section study of CO₂, with a theoretical study of two transitions -- 11. Further developments in the theory and use of the electron spectrometer -- 12. Electronic collision cross sections of water vapor.

Measuring a photonic qubit without destroying it

Measuring the polarization of a single photon typically results in its destruction. We propose, demonstrate, and completely characterize a quantum nondemolition (QND) scheme for realizing such a measurement nondestructively. This scheme uses only linear optics and photodetection of ancillary modes to induce a strong nonlinearity at the single-photon level, nondeterministically. We vary this QND measurement continuously into the weak regime and use it to perform a nondestructive test of complementarity in quantum mechanics. Our scheme realizes the most advanced general measurement of a qubit to date: it is nondestructive, can be made in any basis, and with arbitrary strength.

Entanglement and bifurcations in Jahn-Teller models

We compare and contrast the entanglement in the ground state of two Jahn-Teller models. The Exbeta system models the coupling of a two-level electronic system, or qubit, to a single-oscillator mode, while the Exepsilon models the qubit coupled to two independent, degenerate oscillator modes. In the absence of a transverse magnetic field applied to the qubit, both systems exhibit a degenerate ground state. Whereas there always exists a completely separable ground state in the Exbeta system, the ground states of the Exepsilon model always exhibit entanglement. For the Exbeta case we aim to clarify results from previous work, alluding to a link between the ground-state entanglement characteristics and a bifurcation of a fixed point in the classical analog. In the Exepsilon case we make use of an ansatz for the ground state. We compare this ansatz to exact numerical calculations and use it to investigate how the entanglement is shared between the three system degrees of freedom.

Quantum nondemolition measurement of Fock states of mesoscopic mechanical oscillators

We investigate a scheme that makes a quantum nondemolition (QND) measurement of the excitation level of a mesoscopic mechanical oscillator by utilizing the anharmonic coupling between two beam bending modes. The nonlinear coupling between the two modes shifts the resonant frequency of the readout oscillator in proportion to the excitation level of the system oscillator. This frequency shift may be detected as a phase shift of the readout oscillation when driven on resonance. We derive an equation for the reduced density matrix of the system oscillator, and use this to study the conditions under which discrete jumps in the excitation level occur. The appearance of jumps in the actual quantity measured is also studied using the method of quantum trajectories. We consider the feasibility of the scheme for experimentally accessible parameters.

Ion trap transducers for quantum electromechanical oscillators

An enduring challenge for contemporary physics is to experimentally observe and control quantum behavior in macroscopic systems. We show that a single trapped atomic ion could be used to probe the quantum nature of a mesoscopic mechanical oscillator precooled to 4 K, and furthermore, to cool the oscillator with high efficiency to its quantum ground state. The proposed experiment could be performed using currently available technology.

Modulating the rate and rhythmicity of perceptual rivalry alternations with the mixed 5-HT2A and 5-HT1A agonist psilocybin

Binocular rivalry occurs when different images are presented simultaneously to corresponding points within the left and right eyes. Under these conditions, the observer's perception will alternate between the two perceptual alternatives. Motivated by the reported link between the rate of perceptual alternations, symptoms of psychosis and an incidental observation that the rhythmicity of perceptual alternations during binocular rivalry was greatly increased 10 h after the consumption of LSD, this study aimed to investigate the pharmacology underlying binocular rivalry and to explore the connection between the timing of perceptual switching and psychosis. Psilocybin (4-phosphoryloxy-N,N-dimethyltryptamine, PY) was chosen for the study because, like LSD, it is known to act as an agonist at serotonin (5-HT)(1A) and 5-HT2A receptors and to produce an altered state sometimes marked by psychosis-like symptoms. A total of 12 healthy human volunteers were tested under placebo, low-dose ( 115 mg/kg) and high-dose ( 250 mg/kg) PY conditions. In line with predictions, under both low- and high-dose conditions, the results show that at 90 min postadministration ( the peak of drug action), rate and rhythmicity of perceptual alternations were significantly reduced from placebo levels. Following the 90 min testing period, the perceptual switch rate successively increased, with some individuals showing increases well beyond pretest levels at the final testing, 360 min postadministration. However, as some subjects had still not returned to pretest levels by this time, the mean phase duration at 360 min was not found to differ significantly from placebo. Reflecting the drug-induced changes in rivalry phase durations, subjects showed clear changes in psychological state as indexed by the 5D-ASC ( altered states of consciousness) rating scales. This study suggests the involvement of serotonergic pathways in binocular rivalry and supports the previously proposed role of a brainstem oscillator in perceptual rivalry alternations and symptoms of psychosis.

Nonclassicality of a photon-subtracted Gaussian field

We investigate the nonclassicality of a photon-subtracted Gaussian field, which was produced in a recent experiment, using negativity of the Wigner function and the nonexistence of well-behaved positive P function. We obtain the condition to see negativity of the Wigner function for the case including the mixed Gaussian incoming field, the threshold photodetection and the inefficient homodyne measurement. We show how similar the photon-subtracted state is to a superposition of coherent states.

Rainfall variability at decadal and longer time scales: signal or noise?

Rainfall variability occurs over a wide range of temporal scales. Knowledge and understanding of such variability can lead to improved risk management practices in agricultural and other industries. Analyses of temporal patterns in 100 yr of observed monthly global sea surface temperature and sea level pressure data show that the single most important cause of explainable, terrestrial rainfall variability resides within the El Nino-Southern Oscillation (ENSO) frequency domain (2.5-8.0 yr), followed by a slightly weaker but highly significant decadal signal (9-13 yr), with some evidence of lesser but significant rainfall variability at interclecadal time scales (15-18 yr). Most of the rainfall variability significantly linked to frequencies tower than ENSO occurs in the Australasian region, with smaller effects in North and South America, central and southern Africa, and western Europe. While low-frequency (LF) signals at a decadal frequency are dominant, the variability evident was ENSO-like in all the frequency domains considered. The extent to which such LF variability is (i) predictable and (ii) either part of the overall ENSO variability or caused by independent processes remains an as yet unanswered question. Further progress can only be made through mechanistic studies using a variety of models.

Bright tripartite entanglement in triply concurrent parametric oscillation

We show that an optical parametric oscillator based on three concurrent chi((2)) nonlinearities can produce, above threshold, bright output beams of macroscopic intensities which exhibit strong tripartite continuous-variable entanglement. We also show that there are two ways that the system can exhibit a three-mode form of the Einstein-Podolsky-Rosen paradox, and calculate the extracavity fluctuation spectra that may be measured to verify our predictions.