Interview with Robert Plak Pawilnga

Robert Plak Pawilnga is a Papua New Guinean who studied at the University of Queensland in 2003-2004. He studied on an Australian Development Scholarship (ADS) and completed a Masters in Agricultural Studies. The interview was conducted in English on 9 September 2014 by Dr. Jonathan Ritchie of Deakin University and Dr. Musawe Sinebare of the Pacific Adventist University. This set comprises: an interview recording, a timed summary, and a photograph.

The PTEX component EXP2 is critical for establishing a patent malaria infection in mice

Export of most malaria proteins into the erythrocyte cytosol requires the Plasmodium Translocon of Exported proteins (PTEX) and a cleavable Plasmodium Export Element (PEXEL). In contrast, the contribution of PTEX in the liver stages and export of liver stage proteins is unknown. Here, using the FLP/FRT conditional mutatagenesis system, we generate transgenic P. berghei parasites deficient in EXP2, the putative pore-forming component of PTEX. Our data reveal that EXP2 is important for parasite growth in the liver and critical for parasite transition to the blood, with parasites impaired in their ability to generate a patent blood-stage infection. Surprisingly, whilst parasites expressing a functional PTEX machinery can efficiently export a PEXEL-bearing GFP reporter into the erythrocyte cytosol during a blood stage infection, this same reporter aggregates in large accumulations within the confines of the parasitophorous vacuole membrane during hepatocyte growth. Notably HSP101, the putative molecular motor of PTEX, could not be detected during the early liver stages of infection, which may explain why direct protein translocation of this soluble PEXEL-bearing reporter or indeed native PEXEL proteins into the hepatocyte cytosol has not been observed. This suggests that PTEX function may not be conserved between the blood and liver stages of malaria infection. This article is protected by copyright. All rights reserved.

Robert Cornell, Technical Editor, Electronic Technician Magazine

New York Trade School graduate Robert Cornell is pictured at his desk in his position of Technical Editor, Electronic Technician Magazine. Original caption reads "Robert Cornell - Advanced Television 1954, was appointed Technical Editor of Electronic Technician Magazine in 1956 and has served with distinction since. He is President of CETA (Certified Electronic Technicians Association), which consists of alumni of the New York Trade School who are graduates of the Advanced Television Course. He was recently invited to serve a a member of the Attorney General's Committee seeking to establish safeguards to protect the public in the field of television servicing." Black and white photograph with caption to glued to reverse.