Effects of culture treatments on HUFA levels in Artemia cysts

Artemia cysts (of GSL, Utah, USA origin) were produced from the modified traditional solar salt works of Bangladesh during winter months through different feeding/fertilization treatments (T1, T2, T3, T4 ) were analyzed to understand the effects of treatments on their fatty acid profile. Palmitic, Linolenic, Eicosapantaenonic and Docohexaenoic acids (mg/g. DW) were found highest for the cysts in T1 (16.0% ±1 .36), T2 (14.7% ±0.47), T3 (4.7% ±0.40) and T4 (0.7% ±0.06) treatments, respectively. High amount of 18:3(n-3) acids in the cysts of all sources proves to be freshwater type of the cysts. The presence of marine type essential fatty acids in the cysts of all sources were found low for 20:5n-3 (3.7-4.7%) and very low for 22:6n-3 (0.09-0.7%). No significant variation was observed for 16:0 acids within the treatments, but for 18:3(n-3) acid, the variation was found highly significant (P= 0.0052) between T2 and T4 treatments. For 20:5(n-3), only variation between T2 and T4 was found insignificant (P=0.1161), but between other treatments, significant variation was observed between T2 and T4 (P=0.0241), T2 and T4 (P=0.0022) and T1 andT4 (P=0.0161). No significant variation was found in other treatments.

Effect of mola (Amblypharyngodon mola Ham.) on the growth and production of carps in polyculture

The effect of introduction of mola (Amblypharyngodon mala) in polyculture with rohu (Labeo rohita), catla (Catla catla) and mirror carp (Cyprinus carpio var. specularis) was studied in semi-intensive culture system in the pond complex of the Fisheries Faculty, Bangladesh Agricultural University, Mymensingh. Mala, a small indigenous fish was found to exert a negative impact on growth and production of carps. After four months' rearing, significant difference (P<0.05) was observed in the net production between the treatments.

Studies on the induced breeding and post-larval rearing of shing (Heteropneustes fossilis Bloch)

An experiment was conducted on induced breeding and fry rearing of shing, Heteropneustes fossilis (Bloch) in the Department of Aquaculture, Bangladesh Agricultural University for a period of four months from April to July 1994. Hatching rate was calculated at 21.50h and was found to be 45 to 55 % and the survival rate of larvae was 30 to 40 % at 26 to 29°C. Survival rate and growth rate of post larvae were found to be 50 to 60% and 96.6 to 117.2% respectively. Feed-3 (F3) showed the highest survival rate and growth rate of post larvae.

Investigation on bacterial flora of farmed freshwater prawn (Macrobrachium rosenbergii de Man) in Bangladesh

Total bacterial load in the haemolymph of freshwater prawn Macrobrachium rosenbergii varied from 6.2x10⁴ to 1.9x10⁷ CFU/ml whereas in the hepatopancreas, bacterial load varied from 1.9x10³ to 2.9x10⁵ CFU/g. The total bacterial load in the pond water varied from 2.6x10² to 4.1x10⁵ CFU/ml. The isolated bacterial genera in the haemolymph and the hepatopancreas of prawn were Streptococcus, Acinetobacter, Micrococcus, Aeromonas, Vibrio, Flavobacterium, Staphylococcus and Pseudomonas, whereas the detected bacterial genera in pond water were Micrococcus, Streptococcus, Vibrio, Flavobacterium, Staphylococcus, Pseudomonas and Aeromonas. Among the detected genera, Vibrio and Staphylococcus were found to be dominant genera in the haemolymph of the sampled prawn throughout the study period whereas Staphylococcus and Pseudomonas were dominant in pond water.

Suitability of rich-fish culture under mono and polyculture systems in the boro rice ecosystem

An experiment was conducted in farmers’ fields under Paikgacha thana, Khulna to study the suitability of integrated rice-cum-fish culture. Three treatments namely T1 (Puntius gonionotus), T2 (Puntius gonionotus and Cyprinus carpio) and T3 ( Cyprinus carpio) were included for the study each having three replicates. The fish were stocked at a density of 3750/ha in all the rice plots. The physicochemical parameters of water viz., water depth, temperature, dissolved oxygen, pH, salinity, nitrate and phosphate etc. recorded during the study period were found within optimum range. Of the two cultured species C. carpio attained the highest average individual weight (160g) and survival (81.06%). With respect to biomass and income, highest average fish production and net profit per hectare (306.74kg and Tk. 8177.91) were obtained in T2 and the lowest (184.17kg and Tk. 2049.41) obtained in T1 and a significant variation (p<0.05) in fish production was observed among the treatments while for rice production, it was insignificant. The cost benefit ratio of fish production found were 1:1.29, 1:2.14 and 1:1.90 for T1, T2 and T3, respectively.

Studies on the gonadosomatic index and fecundity of chapila (Gudusia chapra Ham.)

The study was conducted in pond to determine the fecundity and gonadosomatic index of chapila, Gudusia chapra. The male fish was found to attain sexual maturity at 7.7 cm and 7.41 g and that of the female at 9.3 cm and 14.65 g by standard-length and body-weight respectively. The investigated fishes were found to be male and female at the ratio of 1:3 and generally female was found to be larger than male. The fish was found to spawn for several months with two spawning peaks, one in April and another in August as indicated by the peaks of gonadosomatic index and ova diameter. Fecundity of the fish ranged from 25,220 to 154,528 with an average value of 72,383 and was found to increase with the increase in length and weight of the fish. The relationships between fecundity and standard-length, body-weight, gonad-length and gonad weight of the fish were found to be linear and significant.

Limnological conditions of naturally turbid ponds and its effects on fish growth

An experiment was conducted on six naturally turbid ponds in the village of Salakandi situated to the south-west corner of Bangladesh Agricultural University, Mymensingh for a period of three months from July to September '96. The experiment was performed by studying the physicochemical factors of water and soil, the biological factors such as densities of phytoplankton and zooplankton, and the growth of fishes. During the experimental period water turbidity varied among ponds. The highest value of turbidity was found to be 679±183.6 FTU in pond 1 and the lowest was 158±23.31 FTU in pond 4. The maximum and the minimum water transparency were recorded in the month of July and September respectably. The lowest net weight of fishes was found 28.8 kg/ha/year in pond 1 due to high turbidity and the highest was 35.8 kg/ha/year in pond 4 due to low turbidity. Most of the correlations between turbidity and transparency, phytoplankton, and zooplankton were significant at 1% and 5% levels.

Environmental parameters and incidence of white spot disease in Penaeus monodon (Fab.) farming

An investigation was carried out to monitor management practices and to find out whether there is any relationship with occurrence of deadly white spot disease and environmental parameters. Three semi-intensive and a improved traditional shrimp farms were selected in which mass mortality of shrimp (Penaeus monodon) by white spot disease occurred previously. The farms were situated at two different geographical locations. Two ponds from each farm at random were selected for the study. Out of eight investigated ponds, 6 ponds in three farms were affected by the disease during investigation period. The non-affected ponds had relatively lower stocking density, lightly different management practice and were located at different geographical area. There was no significant variation in water quality parameters among the affected and non-affected ponds. No significant variations were recorded in pond preparation, source of Post Larvae (PL), water and feed management among the affected and non-affected ponds. The observation indicated that pond micro-organisms in a farm may not the only cause of the disease but some external factors also might be responsible for the outbreak of this disease.

Study on the intestinal bacteria of Labeo rohita (Ham.)

The quantitative and qualitative aspects of intestinal bacteria of rohu fish (Labeo rohita) showed that total viable count of bacteria ranged from 9.9 x 106 to 1.4 x 107 CFU/g of intestine in different age groups of fish. The bacterial load was highest in the month of July and lowest in January. The genera of the isolates from intestine included Coryneform, Micrococcus, Flavobacterium, Cytophaga, Achromobacter, Aeromonas Enterobacteriaceae and Vibrio. Coryneform was the dominant group throughout the study period followed by Micrococcus and Enterobacteriaceae. Marked variations in the bacterial load and generic composition of intestinal bacteria were evident during the study period in different age groups of rohu fish.

Length-weight relationship and condition factor of black carp (Mylopharyngodon spiceus Richardson) in Bangladesh

The length-weight relationship and condition factor of Mylopharyngodon spiceus were determined. The result of the study showed the dependence of weight (W) on the total length (L) in the following form: W= 0.006L(super 3.156) or in the logarithmic form Log W=- 2.1851 + 3.156 Log L. Standard errors of length and weight were 0.674 cm and 3.214 g respectively. The co-efficient correlation "r" was found to be 0.972 which indicated that the relationship between length and body weight of the fish was highly significant. The t-test also indicated that the correlation between length and weight was significant. The range and mean value of condition factor (K) were 0.865 to 1.041 and 0.958 respectively.

Colossal loss of shell and fin-fish larvae during collection of Penaeus monodon (Fab.) fry in the rivers of Satkhira

This study was conducted in five river-estuaries of Satkhira from January to December '96. It was found that during the collection of each Peneaus monodon) post larva (PL), about 45 larvae of other shrimps, 12 individuals of fin-fishes and 530 macro-zooplankters were mercilessly destroyed. It was also recorded that about 11.6 million of P. monodon PLs were harvested annually from the study area. The sh1dy implies that colossal loss of shell and fin-fishes and other plankton resources is done by tiger shrimp fry collectors, and such massive destruction adversely affect the natural productivity and ecological balance of the coastal environment.

Predation by Channa striatus (Bloch) on Rana tigrina (Daudin), Puntius gonionotus (Bleekeer) and Labeo rohita (Hamilton) in the laboratory

The growth performance of a predatory snakehead, Channa striatus was tested by supplying tadpoles of Rana tigrina and fingerlings of Puntius gonionotus and Labeo rohita as prey for a period of 21 days in aquaria. Prey consumption by C. striatus was significantly different (P<0.05) for different prey used (T1 - R. tigrina, T2 - P. gonionotus, T3 - L. rohita). Tadpoles of R. tigrina were preferred by the predator (C. striatus) over P. gonionotus and L. rohita although tadpole is nutritionally inferior to each of P. gonionotus and L. rohita. Each predator rayed on 50-330 mg per day per g of their body weight. Fish preyed on tadpoles also showed the highest growth. Significant difference in weight gain was found between T1 and T2 and also between T1 and T3 but no difference was found between T2 and T3. Food conversion ratio (FCR) was found to be lowest in treatment T3 followed by the treatments T2 and T1 respectively.

Production of Thai sharpunti (Puntius gonionotus Bleeker) in polyculture with carps using low-cost feed

The effectiveness of duckweed and rice bran as a low cost supplementary feed was compared through a six months production trial of rajpunti (Barbodes gonionotus) with carps. Six earthen ponds of 360 m² each were used for the trial. Three ponds received duckweed, while the other three received rice bran as supplementary feed. Fish biomass after six months of rearing increased to an average of 2,056 kg/ha in ponds which received duckweed and 2,056 kg/ha in rice bran treated ponds. The net profit with duckweed and rice bran worked out to Tk. 69.752 and Tk. 73.480 kg/ha, respectively. This study revealed that duckweed is a low cost supplementary feed, particularly for farmers with limited income.

Minimization of cannibalism of African catfish (Clarias gariepinus Burchell) larvae in indoor culture system

For minimizing cannibalism of African catfish (Clarias gariepinus) larvae two trials for a period of 14 and 15 days respectively in four aquaria of size 120x49x32 cm³ were conducted. Seven days old African catfish larvae with an initial total length and weight of 7.84 (±0.40) mm and 4.40 (±1.18) mg respectively in the first trial and similarly 7.52(±0.61) mm and 3.98 (±0.56) mg in the second trial at the rate of same stocking densities of 2500 larvae in each aquarium were stocked in both trials. Cannibalistic larvae were separated by using grader frame from each treatment at 7 days and 5 days interval during first and second trial respectively. Two mesh sizes i.e., 5 mm and 7 mm were used in the grader frame in both trials. Survival rate was significantly higher in T1 than that of T2 in each trial. Grading of larvae with 5 days interval resulted higher survival rate than that of 7 days interval.

Pathogenicity of Aeromonas hydrophila artificially infected to Indian major carp (Cirrhinus mrigala Ham.)

Abrasion, feeding, injection and immersion methods were used to evaluate the pathogenicity of five different strains of Aeromonas hydrophila viz. RG (rui gill), ML (mrigal lesion), SG (sharpunti gill), F1K (mrigal kidney), GFL (gold fish lesion) and Ah-19 (Aeromonas hydrophila-19, Ref. Strain) against C. mrigala H. Bacterial suspension containing viable cells of 7.5x 10⁵ per ml was found to be very effective in intramuscular injection and feeding resulting 100% mortality after 96hr of inoculation. The strain RG, ML and F1K produced scale loss with erosion of the skin surface with/without hemorrhagic lesion after 48hr of inoculation following abrasion method. The strains SG and Ah-19 resulting scale loss with erosion of the skin surface with/without hemorrhagic lesion after 72hr of inoculation following abrasion and injection methods. SG and F1K caused reddening in mouth region after 72hr of feeding inoculation, whereas RG resulted frank ulcers from eroded dermal layer exposing underlying musculature which was hemorrhagic after 96hr of inoculation by abrasion method.