Mitochondrial DNA-like sequence in the nuclear genome of Saguinus (Callitrichinae, Primates): transfer estimation

Seqüências tipo mitocondriais têm comumente sido encontradas no genoma nuclear de diversos organismos. Quando acidentalmente incluídas em estudos de seqüências mitocondriais, diversas conclusões errôneas podem ser obtidas. No entanto, estes pseudogenes nucleares tipo mitocondriais podem ser usados para a estimativa da taxa relativa de evolução de genes mitocondriais e também como grupo externo em análises filogenéticas. No presente trabalho, seqüências mitocondriais com características do tipo de pseudogene, tais como deleções e/ou inserções e códons de parada, foram encontradas em tamarins (Saguinus spp., Callitrichinae, Primates). A análise filogenética permitiu a estimativa do tempo da migração da seqüência mitocondrial para o genoma nuclear e algumas inferências filogenéticas. A escolha de um grupo externo não adequado (Aotus infulatus) não permitiu uma reconstrução filogenética confiável da subfamília Callitrichinae. A divergência bastante antiga de Cebidae (Callitrichinae, Aotinae e Cebinae) pode ter favorecido o aparecimento de homoplasias, obscurecendo a análise.

Sequence characterization of coding regions of the myostatin gene (GDF8) from Brazilian Murrah buffaloes (Bubalus bubalis) and comparison with the Bos taurus sequence

Within about 30 years the Brazilian buffalo (Bubalus bubalis) herd will reach approximately 50 million head as a result of the great adaptive capacity of these animals to tropical climates, together with the good productive and reproductive potential which make these animals an important animal protein source for poor and developing countries. The myostatin gene (GDF8) is important in the physiology of stock animals because its product produces a direct effect on muscle development and consequently also on meat production. The myostatin sequence is known in several mammalian species and shows a high degree of amino acid sequence conservation, although the presence of non-silent and silent changes in the coding sequences and several alterations in the introns and untranslated regions have been identified. The objective of our work was to characterize the myostatin coding regions of B. bubalis (Murrah breed) and to compare them with the Bos taurus regions looking for variations in nucleotide and protein sequences. In this way, we were able to identify 12 variations at DNA level and five alterations on the presumed myostatin protein sequence as compared to non double-muscled bovine sequences.

Characterization, subsite mapping and N-terminal sequence of miliin, a serine-protease isolated from the latex of Euphorbia milii

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Delimiting the Origin of a B Chromosome by FISH Mapping, Chromosome Painting and DNA Sequence Analysis in Astyanax paranae (Teleostei, Characiformes)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chromosomal Mapping of Repetitive DNA and Cytochrome C Oxidase I Sequence Analysis Reveal Differentiation among Sympatric Samples of Astyanax fasciatus (Characiformes, Characidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ab initio protein folding simulations using atomic burials as informational intermediates between sequence and structure

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Production lot sizing and scheduling with non-triangular sequence-dependent setup times

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Identification and sequence analysis of five allexiviruses species infecting garlic crops in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

An Efficient Parallel Algorithm for Multiple Sequence Similarities Calculation Using a Low Complexity Method

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Overlapped Sequence Types (STs) and Serogroups of Avian Pathogenic (APEC) and Human Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Isolated in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phylogenetic relationships of Malassezia species based on multilocus sequence analysis

Members of the genus Malassezia are lipophilic basidiomycetous yeasts, which are part of the normal cutaneous microbiota of humans and other warm-blooded animals. Currently, this genus consists of 14 species that have been characterized by phenetic and molecular methods. Although several molecular methods have been used to identify and/or differentiate Malassezia species, the sequencing of the rRNA genes and the chitin synthase-2 gene (CHS2) are the most widely employed. There is little information about the beta-tubulin gene in the genus Malassezia, a gene has been used for the analysis of complex species groups. The aim of the present study was to sequence a fragment of the beta-tubulin gene of Malassezia species and analyze their phylogenetic relationship using a multilocus sequence approach based on two rRNA genes (ITS including 5.8S rRNA and D1/D2 region of 26S rRNA) together with two protein encoding genes (CHS2 and beta-tubulin). The phylogenetic study of the partial beta-tubulin gene sequences indicated that this molecular marker can be used to assess diversity and identify new species. The multilocus sequence analysis of the four loci provides robust support to delineate species at the terminal nodes and could help to estimate divergence times for the origin and diversification of Malassezia species.

RESPONSE OF CORN GROWN IN TILLAGE SYSTEM FOR THE APPLICATION OF NITROGEN DOSES IN A SEQUENCE OF PLANT COVER

The use of cover crops can produce large amounts of biomass, improving the cycling of nutrients, particularly nitrogen, promoting productivity gains and cost savings. Given this, the objective was to evaluate the use of N rates associated to cover crops grown in pre-harvest nutritional status, nitrogen accumulation and corn yield in both years. The experiment was conducted in an Oxisol with maize, no-tillage system. The experimental design was a randomized block, split plot with four replications. The main treatments were: six cropping systems (sun hemp, jack bean, lablab, millet, and velvet bean fallow) in secondary treatments: four doses of nitrogen (0, 60, 120 and 180 kg ha(-1) N). Corn yield was not affected by the type of coverage for pre-season, regardless of the nitrogen applied in the soil. Still, the use of nitrogen fertilizer in the soil promotes gains in grain yield in the first year of cultivation, regardless of the type of coverage in pre-season. In the first year (2006/2007) the species of coverage produced more biomass were velvet bean, jack bean, sun hemp and lablab, while in the second year (2007/08) were the sun hemp, millet, lablab, jack bean and velvet bean, respectively.

A BCH code and a sequence of cyclic codes

This study establishes that for a given binary BCH code C0 n of length n generated by a polynomial g(x) ∈ F2[x] of degree r there exists a family of binary cyclic codes {Cm 2m−1(n+1)n}m≥1 such that for each m ≥ 1, the binary cyclic code Cm 2m−1(n+1)n has length 2m−1(n + 1)n and is generated by a generalized polynomial g(x 1 2m ) ∈ F2[x, 1 2m Z≥0] of degree 2mr. Furthermore, C0 n is embedded in Cm 2m−1(n+1)n and Cm 2m−1(n+1)n is embedded in Cm+1 2m(n+1)n for each m ≥ 1. By a newly proposed algorithm, codewords of the binary BCH code C0 n can be transmitted with high code rate and decoded by the decoder of any member of the family {Cm 2m−1(n+1)n}m≥1 of binary cyclic codes, having the same code rate.