O pragmatismo antiteórico de Richard A. Posner e as respostas da teoria moral para a decisão judicial

Este trabalho apresenta uma análise crítica à forma de se abordar casos jurídicos e proferir decisões judiciais denominada abordagem judicial pragmática, disseminada pelo magistrado e professor norte-americano Richard A. Posner. O objetivo é explicitar suas principais características e contornos, bem como sua repulsa pela teorização abstrata e pelos debates e argumentos morais na decisão judicial. A partir disso, pretende-se refutar parte dessa abordagem pragmática, por meio de argumentos levantados por filósofos morais e profissionais do direito a saber: Ronald Dworkin, Charles Fried, Anthony Kronman, John T. Noonan Jr e Martha C. Nussbaum - em defesa de uma abordagem que prega a inevitável utilização do raciocínio teórico, assim como a argumentação e reflexão moral na resolução de casos difíceis relacionados ao direito. Também será destacado como a repulsa pragmática pela teoria moral e abstrata é incompatível com a conjuntura justeórica contemporânea e como a análise de alguns casos difíceis expõe a falibilidade, ainda que parcial, desse estilo de abordagem pregado por Posner.

Global transcriptional responses of the toxic cyanobacterium, Microcystis aeruginosa, to nitrogen stress, phosphorus stress, and growth on organic matter

Whole transcriptome shotgun sequencing (RNA-seq) was used to assess the transcriptomic response of the toxic cyanobacterium Microcystis aeruginosa during growth with low levels of dissolved inorganic nitrogen (low N), low levels of dissolved inorganic phosphorus (low P), and in the presence of high levels of high molecular weight dissolved organic matter (HMWDOM). Under low N, one third of the genome was differentially expressed, with significant increases in transcripts observed among genes within the nir operon, urea transport genes (urtBCDE), and amino acid transporters while significant decreases in transcripts were observed in genes related to photosynthesis. There was also a significant decrease in the transcription of the microcystin synthetase gene set under low N and a significant decrease in microcystin content per Microcystis cell demonstrating that N supply influences cellular toxicity. Under low P, 27% of the genome was differentially expressed. The Pho regulon was induced leading to large increases in transcript levels of the alkaline phosphatase phoX, the Pst transport system (pstABC), and the sphX gene, and transcripts of multiple sulfate transporter were also significantly more abundant. While the transcriptional response to growth on HMWDOM was smaller (5–22% of genes differentially expressed), transcripts of multiple genes specifically associated with the transport and degradation of organic compounds were significantly more abundant within HMWDOM treatments and thus may be recruited by Microcystis to utilize these substrates. Collectively, these findings provide a comprehensive understanding of the nutritional physiology of this toxic, bloom-forming cyanobacterium and the role of N in controlling microcystin synthesis.