In Die Parasceves De Meditatione Passionis Mortis D. N. I. C. Carmen

A. E. D.

Molecular studies of human high grade B cell non -Hodgkin's lymphomas

The non-Hodgkin's B cell lymphomas are a diverse group of neoplastic diseases. The incidence rate of the malignant tumors has been rising rapidly over the past twenty years in the United States and worldwide. The lack of insight to pathogenesis of the disease poses a significant problem in the early detection and effective treatment of the human malignancies. These studies attempted to investigate the molecular basis of pathogenesis of the human high grade B cell non-Hodgkin's lymphomas with a reverse genetic approach. The specific objective was to clone gene(s) which may play roles in development and progression of human high grade B cell non-Hodgkin's lymphomas.^ The messenger RNAs from two high grade B cell lymphoma lines, CJ and RR, were used for construction of cDNA libraries. Differential screening of the derived cDNA libraries yielded a 1.4 kb cDNA clone. The gene, designated as NHL-B1.4, was shown to be highly amplified and over-expressed in the high grade B cell lymphoma lines. It was not expressed in the peripheral blood lymphoid cells from normal donors. However, it was inducible in peripheral blood T lymphocytes by a T cell mitogen, PHA, but could not be activated in normal B cells by B cell mitogen PMA. Further molecular characterization revealed that the gene may have been rearranged in the RR and some other B cell lymphoma lines. The coding capacity of the cDNA has been confirmed by a rabbit reticulocyte lysate and wheat germ protein synthesis system. A recombinant protein with a molecular weight of approximate 30 kDa was visualized in autoradiogram. Polyclonal antisera have been generated by immunization of two rabbits with the NHL-B1.4 recombinant protein produced in the E. coli JM109. The derived antibody can recognize a natural protein with molecular weight of 49 kDa in cell lysate of activated peripheral T lymphocytes of normal donors and both the cell lysate and supernatant of RR B cell lymphoma lines. The possible biologic functions of the molecule has been tested preliminarily in a B lymphocyte proliferation assay. It was found that the Q-sepharose chromatograph purified supernatant of COS cell transfection could increase tritiated thymidine uptake by B lymphocytes but not by T lymphocytes. The B cell stimulatory activity of the supernatant of COS cell tranfection could be neutralized by the polyclonal antisera, indicating that the NHL-B1.4 gene product may be a molecule with BCGF-like activity.^ The expression profiles of NHL-B1.4 in normal and neoplastic lymphoid cells were consistent with the current B lymphocyte activation model and autocrine hypothesis of high grade B cell lymphomagenesis. These results suggested that the NHL-B1.4 cDNA may be a disease-related gene of human high grade B cell lymphomas, which may codes for a postulated B cell autocrine growth factor. ^

PML tumor suppressor potentiates cell death through inhibition of the NF -kappa B survival pathway

The promyelocytic leukemia protein PML is a growth suppressor essential for induction of apoptosis by diverse apoptotic stimuli. The mechanism by which PML regulates cell death remains unclear. In this study we found that ectopic expression of PML potentiates cell death in the TNFα-resistant tumor line U2OS and significantly sensitized these cells to apoptosis induced by TNFα in a p53-independent manner. Our study demonstrated that both PML and PML/TNFα-induced cell death are associated with DNA fragmentation, activation of caspase-3, -7, -8, and degradation of DFF/ICAD. Furthermore, we found that PML-induced and PML/TNFα-induced cell death could be blocked by the caspase-8 inhibitors crmA and c-FLIP, but not by Bcl-2, the inhibitor of mitochondria-mediated apoptotic pathway. These findings indicate that this cell death event is initiated through the death receptor-dependent apoptosis pathway. Our study further showed that PML recruits NF-kappa B (NF-κB) to the PML nuclear body, blocks NF-κB binding to its cognate enhancer, and represses its transactivation function with the C-terminal region. Therefore PML inhibits the NF-κB survival pathway. Overexpression of NF-κB rescued cell death induced by PML and PML/TNFκ. These results imply that PML is a functional repressor of NF-κB. This notion was further supported by the finding that the PML−/− mouse embryo fibroblasts (MEFs) are more resistant than the wild-type MEFs to TNFκ-induced apoptosis. In conclusion, our studies convincingly demonstrated that PML potentiates cell death through inhibition of the NF-κB survival pathway. Activation of NF-κB frequently occurs during oncogenesis. Our study here suggests that a loss of PML function enhances the NF-κB survival pathway and this event may contribute to tumorigenesis. ^

(Table 3) Major element content of whole-rock samples of DSDP Leg 30 holes

All norms were calculated with an atomic ratio of Fe+3/Fe+2 = 0.2, except analysis 7 which was calculated directly from the analysis.

Chemistry of igneous rocks of DSDP Leg 30 holes

Igneous rock units were encountered at four of the five sites drilled on Leg 30 of the Deep Sea Drilling Project. These units uncluded a diabase sill at Site 285, a basalt underlain by a gabbro at 286, two basalt flows at 287, and a basalt flow at 289. Site 285 is located approximately in the center of the South Fiji Basin, Site 286 is adjacent to a filled portion of the New Hebrides Trench, Site 287 is adjacent to a basement high in the Coral Sea Basin, and Sites 288 and 289 are located on the Ontong-Java Plateau north of the Solomon Islands (Figure 1). Figure 2 presents generalized lithologic columns for the igneous rock units found at these sites. When a unit number is given, e.g., Site 286, Unit 4 basalt, this number conforms with the unit number assigned to it in the overall stratigraphic sequence of that hole as defined in the individual Site Reports in this volume. Unless otherwise stated, depths are given as measured from the sediment-igneous rock contact rather than the mudline.

Tramas intergenéricas en el relato Lucaniano del 'día después' (B.C., VII, 786-846)

La escena del campo de batalla tras el enfrentamiento entre pompeyanos y cesarianos en el libro VII del Bellum Ciuile de Lucano (786-846) deja al descubierto el proceso de refuncionalización del tópico tradicional del día después de la lucha. Desde una perspectiva estilística mostramos que la intersección entre los códigos historiográfico, épico y trágico y la manipulación de diversos motivos literarios y culturales en dicho relato pueden leerse como uno de los principales mecanismos de la poética lucaniana del ciuile nefas.

Filón de Alejandría : Obra y pensamiento. Una lectura filológica

Filón de Alejandría (30?a. C.-50? d. C.) es una figura clave para conocer la profunda relación entre Judaísmo y Helenismo en el siglo I d. C. Judío practicante, su profundo dominio de la lengua, la literatura y, de modo especial, la retórica griegas lo convierten en una personalidad extraordinaria de su tiempo, tanto por su exégesis de la Torá (o Pentateuco) como por sus escritos apologéticos, históricos y filosóficos. Su testimonio es de gran valor para todo estudioso de la Antigüedad clásica por los muchos datos que aporta acerca de la filosofía, el pensamiento, la educación y la tradición clásica en el momento histórico que le tocó vivir, precisamente en un centro cultural de excepcional importancia dentro del Imperio romano

Tramas intergenéricas en el relato Lucaniano del 'día después' (B.C., VII, 786-846)

La escena del campo de batalla tras el enfrentamiento entre pompeyanos y cesarianos en el libro VII del Bellum Ciuile de Lucano (786-846) deja al descubierto el proceso de refuncionalización del tópico tradicional del día después de la lucha. Desde una perspectiva estilística mostramos que la intersección entre los códigos historiográfico, épico y trágico y la manipulación de diversos motivos literarios y culturales en dicho relato pueden leerse como uno de los principales mecanismos de la poética lucaniana del ciuile nefas.

Filón de Alejandría : Obra y pensamiento. Una lectura filológica

Filón de Alejandría (30?a. C.-50? d. C.) es una figura clave para conocer la profunda relación entre Judaísmo y Helenismo en el siglo I d. C. Judío practicante, su profundo dominio de la lengua, la literatura y, de modo especial, la retórica griegas lo convierten en una personalidad extraordinaria de su tiempo, tanto por su exégesis de la Torá (o Pentateuco) como por sus escritos apologéticos, históricos y filosóficos. Su testimonio es de gran valor para todo estudioso de la Antigüedad clásica por los muchos datos que aporta acerca de la filosofía, el pensamiento, la educación y la tradición clásica en el momento histórico que le tocó vivir, precisamente en un centro cultural de excepcional importancia dentro del Imperio romano

Tramas intergenéricas en el relato Lucaniano del 'día después' (B.C., VII, 786-846)

La escena del campo de batalla tras el enfrentamiento entre pompeyanos y cesarianos en el libro VII del Bellum Ciuile de Lucano (786-846) deja al descubierto el proceso de refuncionalización del tópico tradicional del día después de la lucha. Desde una perspectiva estilística mostramos que la intersección entre los códigos historiográfico, épico y trágico y la manipulación de diversos motivos literarios y culturales en dicho relato pueden leerse como uno de los principales mecanismos de la poética lucaniana del ciuile nefas.

Filón de Alejandría : Obra y pensamiento. Una lectura filológica

Filón de Alejandría (30?a. C.-50? d. C.) es una figura clave para conocer la profunda relación entre Judaísmo y Helenismo en el siglo I d. C. Judío practicante, su profundo dominio de la lengua, la literatura y, de modo especial, la retórica griegas lo convierten en una personalidad extraordinaria de su tiempo, tanto por su exégesis de la Torá (o Pentateuco) como por sus escritos apologéticos, históricos y filosóficos. Su testimonio es de gran valor para todo estudioso de la Antigüedad clásica por los muchos datos que aporta acerca de la filosofía, el pensamiento, la educación y la tradición clásica en el momento histórico que le tocó vivir, precisamente en un centro cultural de excepcional importancia dentro del Imperio romano