Efectos dinámicos laterales en vehículos y puentes ferroviarios sometidos a la acción de vientos transversales

En las últimas décadas el aumento de la velocidad y la disminución del peso de los vehículos ferroviarios de alta velocidad ha provocado que aumente su riesgo de vuelco. Además, las exigencias de los trazados de las líneas exige en ocasiones la construcción de viaductos muy altos situados en zonas expuestas a fuertes vientos. Esta combinación puede poner en peligro la seguridad de la circulación. En esta tesis doctoral se estudian los efectos dinámicos que aparecen en los vehículos ferroviarios cuando circulan sobre viaductos en presencia de vientos transversales. Para ello se han desarrollado e implementado una serie de modelos numéricos que permiten estudiar estos efectos de una forma realista y general. Los modelos desarrollados permiten analizar la interacción dinámica tridimensional tren-estructura, formulada mediante coordenadas absolutas en un sistema de referencia inercial, en un contexto de elementos _nitos no lineales. Mediante estos modelos se pueden estudiar de forma realista casos extremos como el vuelco o descarrilamiento de los vehículos. Han sido implementados en Abaqus, utilizando sus capacidades para resolver sistemas multi-cuerpo para el vehículo y elementos finitos para la estructura. La interacción entre el vehículo y la estructura se establece a través del contacto entre rueda y carril. Para ello, se han desarrollado una restricción, que permite establecer la relación cinemática entre el eje ferroviario y la vía, teniendo en cuenta los posibles defectos geométricos de la vía; y un modelo de contacto rueda-carril para establecer la interacción entre el vehículo y la estructura. Las principales características del modelo de contacto son: considera la geometría real de ambos cuerpos de forma tridimensional; permite resolver situaciones en las que el contacto entre rueda y carril se da en más de una zona a la vez; y permite utilizar distintas formulaciones para el cálculo de la tensión tangencial entre ambos cuerpos. Además, se ha desarrollado una metodología para determinar, a partir de formulaciones estocásticas, las historias temporales de cargas aerodinámicas debidas al viento turbulento en estructuras grandes y con pilas altas y flexibles. Esta metodología tiene cuenta la variabilidad espacial de la velocidad de viento, considerando la correlación entre los distintos puntos; considera las componentes de la velocidad del viento en tres dimensiones; y permite el cálculo de la velocidad de viento incidente sobre los vehículos que atraviesan la estructura. La metodología desarrollada en este trabajo ha sido implementada, validada y se ha aplicado a un caso concreto en el que se ha estudiado la respuesta de un tren de alta velocidad, similar al Siemens Velaro, circulando sobre el viaducto del río Ulla en presencia viento cruzado. En este estudio se ha analizado la seguridad y el confort de la circulación y la respuesta dinámica de la estructura cuando el tren cruza el viaducto. During the last decades the increase of the speed and the reduction of the weight of high-speed railway vehicles has led to a rise of the overturn risk. In addition, the design requests of the railway lines require some times the construction of very tall viaducts in strong wind areas. This combination may endanger the traffic safety. In this doctoral thesis the dynamic effects that appear in the railway vehicles when crossing viaducts under strong winds are studied. For this purpose it has been developed and implemented numerical models for studying these effects in a realistic and general way. The developed models allow to analyze the train-structure three-dimensional dynamic interaction, that is formulated by using absolute coordinates in an inertial reference frame within a non-linear finite element framework. By means of these models it is possible to study in a realistic way extreme situations such vehicle overturn or derailment. They have been implemented for Abaqus, by using its capabilities for solving multi-body systems for the vehicle and finite elements for the structure. The interaction between the vehicle and the structure is established through the wheel-rail contact. For this purpose, a constraint has been developed. It allows to establish the kinematic relationship between the railway wheelset and the track, taking into account the track irregularities. In addition, a wheel-rail contact model for establishing the interaction of the vehicle and the structure has been developed. The main features of the contact model are: it considers the real geometry During the last decades the increase of the speed and the reduction of the weight of high-peed railway vehicles has led to a rise of the overturn risk. In addition, the design requests of the railway lines require some times the construction of very tall viaducts in strong wind areas. This combination may endanger the traffic safety. In this doctoral thesis the dynamic effects that appear in the railway vehicles when crossing viaducts under strong winds are studied. For this purpose it has been developed and implemented numerical models for studying these effects in a realistic and general way. The developed models allow to analyze the train-structure three-dimensional dynamic interaction, that is formulated by using absolute coordinates in an inertial reference frame within a non-linear finite element framework. By means of these models it is possible to study in a realistic way extreme situations such vehicle overturn or derailment. They have been implemented for Abaqus, by using its capabilities for solving multi-body systems for the vehicle and finite elements for the structure. The interaction between the vehicle and the structure is established through the wheel-rail contact. For this purpose, a constraint has been developed. It allows to establish the kinematic relationship between the railway wheelset and the track, taking into account the track irregularities. In addition, a wheel-rail contact model for establishing the interaction of the vehicle and the structure has been developed. The main features of the contact model are: it considers the real geometry

Lateral Dynamic Models for High-Speed Railway Bridges and Vehicles

The analysis of the running safety of railway vehicles on viaducts subject to strong lateral actions such as cross winds requires coupled nonlinear vehicle-bridge interaction models, capable to study extreme events. In this paper original models developed by the authors are described, based on finite elements for the structure, multibody and finite element models for the vehicle, and specially developed interaction elements for the interface between wheel and rail. The models have been implemented within ABAQUS and have full nonlinear capabilities for the structure, the vehicle and the contact interface. An application is developed for the Ulla Viaduct, a 105 m tall arch in the Spanish high-speed railway network. The dynamic analyses allow obtaining critical wind curves, which define the running safety conditions for a given train in terms of speed of circulation and wind speed

Activation of Androgen Receptor Function by a Novel Nuclear Protein Kinase

Androgen receptor (AR) belongs to the nuclear receptor superfamily and mediates the biological actions of male sex steroids. In this work, we have characterized a novel 130-kDa Ser/Thr protein kinase ANPK that interacts with the zinc finger region of AR in vivo and in vitro. The catalytic kinase domain of ANPK shares considerable sequence similarity with the minibrain gene product, a protein kinase suggested to contribute to learning defects associated with Down syndrome. However, the rest of ANPK sequence, including the AR-interacting interface, exhibits no apparent homology with other proteins. ANPK is a nuclear protein that is widely expressed in mammalian tissues. Its overexpression enhances AR-dependent transcription in various cell lines. In addition to the zinc finger region, ligand-binding domain and activation function AF1 of AR are needed, as the activity of AR mutants devoid of these domains was not influenced by ANPK. The receptor protein does not appear to be a substrate for ANPK in vitro, and overexpression of ANPK does not increase the extent of AR phosphorylation in vivo. In view of this, it is likely that ANPK-mediated activation of AR function is exerted through modification of AR-associated proteins, such as coregulatory factors, and/or through stabilization of the receptor protein against degradation.

Endogenous, hyperactive Rac3 controls proliferation of breast cancer cells by a p21-activated kinase-dependent pathway

Uncontrolled cell proliferation is a major feature of cancer. Experimental cellular models have implicated some members of the Rho GTPase family in this process. However, direct evidence for active Rho GTPases in tumors or cancer cell lines has never been provided. In this paper, we show that endogenous, hyperactive Rac3 is present in highly proliferative human breast cancer-derived cell lines and tumor tissues. Rac3 activity results from both its distinct subcellular localization at the membrane and altered regulatory factors affecting the guanine nucleotide state of Rac3. Associated with active Rac3 was deregulated, persistent kinase activity of two isoforms of the Rac effector p21-activated kinase (Pak) and of c-Jun N-terminal kinase (JNK). Introducing dominant-negative Rac3 and Pak1 fragments into a breast cancer cell line revealed that active Rac3 drives Pak and JNK kinase activities by two separate pathways. Only the Rac3–Pak pathway was critical for DNA synthesis, independently of JNK. These findings identify Rac3 as a consistently active Rho GTPase in human cancer cells and suggest an important role for Rac3 and Pak in tumor growth.

Molecular signals required for type III secretion and translocation of the Xanthomonas campestris AvrBs2 protein to pepper plants

Strains of Xanthomonas campestris pv. vesicatoria (Xcv) carrying avrBs2 are specifically recognized by Bs2 pepper plants, resulting in localized cell death and plant resistance. Agrobacterium-mediated transient expression of the Xcv avrBs2 gene in plant cells results in Bs2-dependent cell death, indicating that the AvrBs2 protein alone is sufficient for the activation of disease resistance-mediated cell death in planta. We now provide evidence that AvrBs2 is secreted from Xcv and that secretion is type III (hrp) dependent. N- and C-terminal deletion analysis of AvrBs2 has identified the effector domain of AvrBs2 recognized by Bs2 pepper plants. By using a truncated Pseudomonas syringae AvrRpt2 effector reporter devoid of type III signal sequences, we have localized the minimal region of AvrBs2 required for type III secretion in Xcv. Furthermore, we have identified the region of AvrBs2 required for both type III secretion and translocation to host plants. The mapping of AvrBs2 sequences sufficient for type III delivery also revealed the presence of a potential mRNA secretion signal.

Open randomised study of use of levonorgestrel releasing intrauterine system as alternative to hysterectomy

Objectives: To assess whether the levonorgestrel intrauterine system could provide a conservative alternative to hysterectomy in the treatment of excessive uterine bleeding.

Reduced fetal growth rate and increased risk of death from ischaemic heart disease: cohort study of 15 000 Swedish men and women born 1915-29

Objective: To establish whether fetal growth rate (as distinct from size at birth) is associated with mortality from ischaemic heart disease.

Humanitarian Visas: Option or obligation?. CEPS Liberty and Security in Europe No. 68, 27 October 2014

Third-country nationals seeking protection have no EU-wide legal channels at present for entering EU territory and triggering protection mechanisms under the Common European Asylum System. As a result, many embark on hazardous journeys, with concomitant risks and loss of human life. The absence of ‘protection-sensitive’ mechanisms for accessing EU territory, along with EU external and extraterritorial border and migration management and control, undermine Member States' refugee and human rights obligations. Humanitarian visas may offer a remedy in this regard by enabling third-country nationals to apply in situ for entry to EU territory on humanitarian grounds or because of international obligations. This study asks whether the existing Visa Code actually obliges Member States to issue humanitarian visas. It also examines past implementation of humanitarian visa schemes by Member States and considers whether more could be done to encourage them to make use of existing provisions in EU law. Finally, with a Commission proposal for Visa Code reform on the table, it asks whether there is now an opportunity to lay down clear rules for humanitarian visa schemes.

Notice sur le premier ouvrage d'anatomie et de médecine, imprimé en turc, à Constantinople, en 1820. intitulé Miroir des corps dans l'anatomie de l'homme [par Châni-Zadeh] ... suivie du catalogue des livres turcs, arabes et persans, imprimés à Constantinople depuis l'introduction de l'imprimerie, en 1726-27, jusqu'en 1820.

Mode of access: Internet.