Effect of conditioned media, nutritive elements, and mitotic synchronization on the accuracy of the cytogenetic analysis in acute nonlymphocytic leukemia patients presenting with inv(16)/t(16;16) or t(15;17).

To improve the yield of the cytogenetic analysis in patients with acute nonlymphocytic leukemia (ANLL), six culture conditions for bone marrow or peripheral blood cells were tested in parallel. Two conditioned media (CM), phytohemagglutinin leukocyte PHA-LCM and 5637 CM, nutritive elements (NE), and methotrexate (MTX) cell synchronization were investigated in 14 patients presenting with either inv(16)/ t(16;16) (group 1, n = 9 patients) or t(15;17) (group 2, n = 5). The criteria used to identify the most favorable culture conditions were the mitotic index (MI), the morphological index (MorI), and the percentage of abnormal metaphases. In the presence of PHA-LCM and 5637 CM, the MI were significantly increased in group 2, whereas in the MTX conditions, MI remained very low in both groups. The values of the MorI did not reveal any significant changes in chromosome resolution between the conditions in either group. The addition of NE did not have a positive effect in quantity or quality of metaphases. Because of the variability of the response of leukemic cells to different stimulations in vitro, several culture conditions in parallel are required to ensure a satisfactory yield of the chromosome analysis in ANLL.

Administrative Transition Planning Notebook, November 15, 2006

Seven Years ofInnovation, Technological Advances, Enhanced Service & Fluid Commerce in the Iowa Alcoholic Beverages Division.

Fiscal Update, March 15, 2010

The Fiscal Division newsletter, published weekly during session and periodically during the interim.

Iowa Workforce Monthly, April 2010, Issue 15

Highlights: *YouTube and Twitter.....................pg. 2 Stay up-to-date on all the training opportunities available in your area.............................................pg. 2 * RES Success Story: In January, Johanna Hofmeister attended Re-Employment Services (RES) training......................pg. 2 * We are three weeks into the My Iowa UI presentation schedule and response has been tremendous..............................pg. 3 * In 2009, IWD Employees raised over $7,200 for the Toys for Tots program............pg. 3

[Lettre autographe signée de Giuseppe Verdi adressée à Escudier, Tubiano, 15 septembre 1868]

Comprend aussi une enveloppe

The Iowa Perinatal, April-May-June 2004, Vol. 15, no.2

This newsletter from The Department of Public Health about perinatal health care and statistics.

Whose Interest Is Being Served? An Investigation of a Walker City Council Member’s Conflict of Interest, April 15, 2010

The Ombudsman received a complaint concerning Bill Smith, a city council member who is also the Walker Fire Department Chief. The complainant stated that Mr. Smith votes regularly as a city council member on matters before the Walker City Council (Council) pertaining to the Walker Fire Department. These actions were alleged to be conflicts of interest in violation of Iowa law.

Infonet, September 15, 2008, Issue 10

A publication of the Governorʼs DD Council & ID Action. It was first created nearly two decades ago as a print-only publication, sent to a few hundred people. Today we offer our nearly 3,000 direct subscribers and a variety of services.