Investment potentials in shellfish culture in Nigeria

Shellfish are a major but cheap protein source for human consumption as well as source of income for coastal towns and villages of the Niger Delta in Rivers State, Cross River, and Lagos States. A research into the nutritive value of some of these marine shellfish viz: bivalves (oyster - Crassostrea gasar and cockle - Anadara senilis); gastropods (periwinkle - Tympanotonus fuscatus, obtuse periwinkle - Semifusus morio and the giant whelk - Thais callifera) and mangrove crabs (green crab - Goniopsis pelli, ghost crab - Cardisoma ormatum, and common blue crab - Callinectes latimanus) was carried out to compare their quality and cost with beef, chicken meat, pork and egg in order to identify those most suitable for commercial culture. Results show that all shellfish had at least 16% crude protein except blue crab (13.38%). All shellfish had higher protein content than egg (13.36%). Cockle with protein content 25.47% compared favourably with beef, (29.60%). Beef, chicken meat and pork cost 11.50, 9.00 and 8.00 per kilo respectively while oyster, periwinkle and the common blue crab cost 3.50, 3.00, and 1.50 per kilo respectively. Oysters and Cockles are recommended for commercial culture based on the findings of this research

On the interaction of actinomycin and DNA

Experiments have been accomplished that (a) further define the nature of the strong, G-containing DNA binding sites for actinomycin D (AMD), and (b) quantitate the in vitro inhibition of E. coli RNA polymerase activity by T7 DNA-bound AMD.

Twenty-five to forty percent of the G's of crab dAT are disallowed as strong AMD binding sites. The G's are measured to be randomly distributed, and, therefore, this datum cannot be explained on the basis of steric interference alone. Poly dAC:TG binds as much AMD and as strongly as any natural DNA, so the hypothesis that the unique strong AMD binding sites are G and a neighboring purine is incorrect. The datum can be explained on the basis of both steric interference and the fact that TGA is a disallowed sequence for strong AMD binding.

Using carefully defined in vitro conditions, there is one RNA synthesized per T7 DNA by E. coli RNA polymerase. The rate of the RNA polymerase-catalyzed reaction conforms to the equation 1/rate = 1/k_A(ATP) + 1/K_G(GTP) + 1/K_C(CTP) + 1/K_U(UTP) T7 DNA-bound AMD has only modest effects on initiation and termination of the polymerase-catalyzed reaction, but a large inhibitory effect on propagation. In the presence of bound AMD, k_G and k_C are decreased, whereas k_A and k_U are unaffected. These facts are interpreted to mean that on the microscopic level, on the average, the rates of incorporation of ATP and UTP are the same in the absence or presence of bound AMD, but that the rates of incorporation of GTP and CTP are decreased in the presence of AMD.

Hábitos alimentares e análise de sobreposição trófica de elasmobrânquios no litoral sul do Rio de Janeiro, Brasil

O presente trabalho analisou o conteúdo estomacal de cinco espécies de elasmobrânquios, sendo 9 estômagos de Psammobatis rutrum, 83 de Psammobatis extenta, 59 de Atlantoraja cyclophora, 112 de Rioraja agassizi e 43 de Rhizoprionodon lalandii, com o objetivo de obter informações sobre os hábitos alimentares dessas espécies, além de verificar a formação de guildas tróficas entre as espécies estudadas. As coletas ocorreram entre janeiro de 2006 e agosto de 2007, no litoral sul do Rio de Janeiro, ao largo da Ilha Grande. Os itens alimentares encontrados foram analisados quanto a Frequência de Ocorrência, Peso Percentual e Índice Alimentar, sendo a dieta de cada espécie avaliada considerando toda amostra, de acordo com o sexo dos indivíduos, estádios de maturidade e épocas do ano. Para investigar a similaridade entre as dietas e verificar a formação de guildas tróficas foi realizada uma Análise de Escalonamento Multidimensional (MDS). As dietas de Psammobatis rutrum e Psammobatis extenta foram consideradas carcino-bentófagas, com grande importância de camarões. O principal item para Atlantoraja cyclophora foi o siri Portunus spinicarpus, sendo a dieta considerada principalmente carcino-bentófaga, mas também com grande importância de teleósteos. Em Rioraja agassizi, a dieta foi considerada principalmente carcino-bentófaga, mas também com forte influência ictiófaga. Já o comportamento alimentar de Rhizoprionodon lalandii foi considerado ictio-bentófago. A dieta entre os sexos apresentou uma alta sobreposição em P. extenta, A. cyclophora e R. lalandii, mas para R. agassizi essa sobreposição foi considerada baixa. Entre os estádios de maturidade a sobreposição foi pequena para P. extenta e R. agassizi, entretanto para A. cyclophora e R. lalandii essa sobreposição foi elevada. Os exemplares maiores, aparentemente apresentaram preferência a se alimentarem de peixes, enquanto que predadores de menor porte se restringem aos invertebrados, basicamente crustáceos. Para todas as espécies foram identificadas alterações sazonais na importância dos itens alimentares, que pode ser explicado pelas oscilações naturais na composição e abundancia das presas potenciais em consequência das variações das massas de água na plataforma continental do sul fluminense. Os resultados indicaram a formação de 2 guildas, sendo a guilda 1 composta por P. rutrum e P extenta e com uma dieta principalmente de crustáceos (camarões) e a guilda 2 composta por A. cyclophora, R. agassizi e R. lalandii apresentando uma alimentação em Crustáceos e em Teleósteos.

Vulnerabilidade socioambiental dos pescadores e catadores de caranguejo da Baía de Guanabara: o trabalho e a saúde em um contexto de injustiça ambiental

O estudo tem como objetivo compreender as alterações socioambientais e econômicas que exercem grande pressão no ecossistema e que atuam diretamente na vida, trabalho e saúde dos pescadores artesanais e dessa forma promover a discussão sobre a possibilidade do fim dessa atividade artesanal na Baía de Guanabara em um contexto de vulnerabilidade e injustiça ambiental. A atividade pesqueira artesanal desenvolvida durante décadas está ameaçada de acabar principalmente por conta da degradação do ambiente e consequentemente da diminuição progressiva da pesca. Essa situação tem conseqüências sociais, econômicas e ambientais, pois são diferentes comunidades instaladas no entorno da baía que ainda sobrevivem da atividade pesqueira. O estudo tem caráter exploratório e descritivo com metodologia quanti-qualitativa, utilizando entrevistas semiestruturadas em diferentes comunidades pesqueiras da Baía de Guanabara. Foram selecionados três municípios (São Gonçalo, Itaboraí e Magé) entrevistando 100 pescadores e catadores de caranguejo que responderam a questões sobre o trabalho, a vida e a saúde. Como resultados foi possível construir indicadores socioambientais que apontam para a extrema vulnerabilidade das comunidades pesqueiras da região. É necessário que espaços de discussão sejam fortalecidos com a mobilização e atuação das comunidades pesqueiras, para busca da revitalização da baía e uma melhor qualidade de vida da população

Résultats du groupe de travail FAO/COPACE sur l'évaluation des stocks démerseaux côtiers du secteur Côte d'Ivoire - Congo

The exploitation rate of demersal stocks in the Côte d'Ivoire-Congo area is in most cases below the level permitting maximum sustainable yield. Any increase in total catch would be achieved through an increase in catch per effort which implies bigger mesh sizes than those in use now (40-45 mm). A first step would be to fix the minimum legal mesh size to 60 mm. New, probably limited resources (crab, squids, benthic sharks) are to be sought along the continental slope.

Étude du crabe rouge profond Geryon quinquedens en Côte d'Ivoire. 1 - Prospection le long du talus continental: résultats des pêches

From Aug 1975 to Jan 1976 a fishing survey for deep-sea red crab Geryon quinquedens was executed along the Ivorian coast between 3{degree} and 7{degree}30'W. Three regions could be distinguished: a central one with poor catches (1.6 kg/trap/20h); an east region with the best catches (5 kg/trap/20h); and a west region, also with good catches (4.5 kg/trap/20h). For the whole survey, catches only took place between 300 and 700m, the best at 400 m. A distinct sexual segregation was observed according to bathymetry with the percentage of males increasing with depth. Nearly the same abundance was observed in Côte d'Ivoire, in Congo and North Angola, while in South Angola catches are somewhat higher.

Note sur les premiers essais de capture du crabe profond Geryon quinquedens en Côte d'Ivoire

First experiments to trap the deep sea red crab Geryon quinquedens off Côte d'Ivoire have had good success. The yields obtained are similar to those observed off the northeastern coast of the United States and off Angola, where commercial fisheries have been developed for some years.

Climate-related hydrological regimes and their effects on abundance of juvenile blue crabs (Callinectes sapidus) in the northcentral Gulf of Mexico

The abundance of juvenile blue crabs (Callinectes sapidus) in the northcentral Gulf of Mexico was investigated in response to climate-related hydrological regimes. Two distinct periods of blue crab abundance (1, 1973–94 and 2, 1997–2005) were associated with two opposite climaterelated hydrological regimes. Period 1 was characterized by high numbers of crabs, whereas period 2 was characterized by low numbers of crabs. The cold phase of the Atlantic Multidecadal Oscillation (AMO) and high north-south wind momentum were associated with period 1. Hydrological conditions associated with phases of the AMO and North Atlantic Oscillation (NAO) in conjunction with the north-south wind momentum may favor blue crab productivity by influencing blue crab predation dynamics through the exclusion of predators. About 25% (22–28%) of the variability in blue crab abundance was explained by a north–south wind momentum in concert with either salinity, precipitation, or the Palmer drought severity index, or by a combination of the NAO and precip

Evaluation of the capture efficiency and size selectivity of four pot types in the prospective fishery for North Pacific giant octopus (Enteroctopus dofleini)

Over 230 metric tons of octopus is harvested as bycatch annually in Alaskan trawl, long-line, and pot fisheries. An expanding market has fostered interest in the development of a directed fishery for North Pacific giant octopus (Enteroctopus dofleini). To investigate the potential for fishery development we examined the efficacy of four different pot types for capture of this species. During two surveys in Kachemak Bay, Alaska, strings of 16 –20 sablefish, Korean hair crab, shrimp, and Kodiak wooden lair pots were set at depths ranging between 62 and 390 meters. Catch per-unit-of-ef for t estimates were highest for sablefish and lair pots. Sablefish pots caught significantly heavier North Pacific giant octopuses but also produced the highest bycatch of commercially important species, such as halibut (Hippoglossus stenolepis), Pacific cod (Gadus macrocephalus), and Tanner crab (Chionoecetes bairdi).

Prediction of discard mortality for Alaskan crabs after exposure to freezing temperatures, based on a reflex impairment index

Millions of crabs are sorted and discarded in freezing conditions each year in Alaskan fisheries for Tanner crab (Chionoecetes bairdi) and snow crab (C. opilio). However, cold exposures vary widely over the fishing season and among different vessels, and mortalities are difficult to estimate. A shipboard experiment was conducted to determine whether simple behavioral observations can be used to evaluate crab condition after low-temperature exposures. Crabs were systematically subjected to cold in seven different exposure treatments. They were then tested for righting behavior and six different ref lex actions and held to monitor mortality. Crabs lost limbs, showed ref lex impairment, and died in direct proportion to increases in cold exposure. Righting behavior was a poor predictor of mortality, whereas reflex impairment (scored as the sum of reflex actions that were lost) was an excellent predictor. This composite index could be measured quickly and easily in hand, and logistic regression revealed that the relationship between reflex impairment and mortality correctly predicted 80.0% of the mortality and survival for C. bairdi, and 79.4% for C. opilio. These relationships provide substantial improvements over earlier approaches to mortality estimation and were independent of crab size and exposure temperature.

Prosomal-width-to-weight relationships in American horseshoe crabs (Limulus polyphemus): examining conversion factors used to estimate landings

Horseshoe crabs (Limulus polyphemus) are valued by many stakeholders, including the commercial fishing industry, biomedical companies, and environmental interest groups. We designed a study to test the accuracy of the conversion factors that were used by NOAA Fisheries and state agencies to estimate horseshoe crab landings before mandatory reporting that began in 1998. Our results indicate that the NOAA Fisheries conversion factor consistently overestimates the weight of male horseshoe crabs, particularly those from New England populations. Because of the inaccuracy of this and other conversion factors, states are now mandated to report the number (not biomass) and sex of landed horseshoe crabs. However, accurate estimates of biomass are still necessary for use in prediction models that are being developed to better manage the horseshoe crab fishery. We recommend that managers use the conversion factors presented in this study to convert current landing data from numbers to biomass of harvested horseshoe crabs for future assessments.

Activity in the pallial nerve of knobbed (Busycon carica) and channeled (Busycotypus canaliculatum) whelks recorded during exposure of the osphradium to odorant solutions

Adult horseshoe crabs (Limulus polyphemus) are the preferred bait in the U.S. east coast whelk pot fishery, but their harvest is being restricted because of severe population declines in the Chesapeake and Delaware bays. To identify other baits, the activity in the pallial nerve of whelks was determined during exposure of the osphradium to odorant solutions prepared from horseshoe crab eggs, horseshoe crab hemolymph, and hard clam (Mercenaria mercenaria) tissue. All three elicited significant responses; bait based on them may provide an alternative to the use of adult horseshoe crabs, although extensive behavioral testing remains to be done. Channeled whelk did not respond to molecular weight fractions (>3 kDa and <3 kDa) prepared from horseshoe crab egg odorant solutions but did respond when the molecular weight fractions were recombined. Whelks appear to have broadly tuned chemoreceptors and manufactured baits may need to mimic the complex mixture of odorants derived from natural sources.

Abundance of adult horseshoe crabs (Limulus polylphemus) in Delaware Bay estimated from a bay-wide mark-recapture study

Estimates of the abundance of American horseshoe crabs (Limulus polyphemus) are important to determine egg production and to manage populations for the energetic needs of shorebirds that feed on horseshoe crab eggs. In 2003, over 17,500 horseshoe crabs were tagged and released throughout Delaware Bay, and recaptured crabs came from spawning surveys that were conducted during peak spawning. We used two release cohorts to test for a temporary effect of tagging on spawning behavior and we adjusted the number of releases according to relocation rates from a telemetry study. The abundance estimate was 20 million horseshoe crabs (90 % confidence interval: 13−28 million), of which 6.25 million (90% CI: 4.0−8.8 million) were females. The combined harvest rate for Delaware, New Jersey, Virginia, and Maryland in 2003 was 4% (90% CI: 3−6%) of the abundance estimate. Over-wintering of adults in Delaware Bay could explain, in part, differences in estimates from ocean-trawl surveys. Based on fecundity of 88,000 eggs per female, egg production was 5.5×1011 (90% CI: 3.5×1011, 7.7×1011), but egg availability for shorebirds also depended on overlap between horseshoe crab and shorebird migrations, density-dependent bioturbation, and wave-mediated vertical transport.

Potential causes of mortality for horseshoe crabs (Limulus polyphemus) during the biomedical bleeding process

Biomedical companies catch and bleed horseshoe crabs for the production of Limulus amebocyte lysate (LAL), a product used for protecting public health (Berkson and Shuster, 1999). LAL is a clotting agent, derived solely from horseshoe crab blood cells, which is used to detect the presence of pathogenic gramnegative bacteria in injectable drugs and implantable medical and dental devices (Mikkelsen, 1988; Novitsky, 1991). In addition, LAL is used in many diagnostic tests for such illnesses as gram-negative bacterial meningitis and typhoid fever (Ding and Ho, 2001). Because the LAL test allows one to detect femtogram levels of endotoxin (Ding and Ho, 2001), it is the most effective test for detecting endotoxin contamination, and its increasing use in medical and pharmaceutical laboratories makes it a highly valued product.

Integration of crustacean aquaculture with coastal rice farming in Vietnam

Some examples are given of integration of crustacean aquaculture into coastal rice farming in Vietnam. Freshwater prawn (Macrobrachium rosenbergii ) and marine shrimp culture integrated with coastal rice farming and crab (Scylla serrata ) culture in backyard ponds and ricefields are described.