Avaliação do potencial genotóxico e mutagênico do efluente de uma refinaria de petróleo e dos rios sob sua influência, por meio de bioensaios com Oreochromis niloticus (Perciformes, Cichlidae)

The petrochemical industry represents a sector of intense activity, in constant expansion and great economic importance for Brazil. In order to be used in various human activities petroleum needs to suffer a refinement process that, besides requiring large amounts of water, produces large amounts of wastes, which are discharged in hydric resources. Petroleum is a complex mixture mainly comprised by hydrocarbons, many of them are recognized as toxic chemicals, which are able to induce a considerable environmental pollution. Since water is an important resource for the maintenance of ecosystems and is also the final receiver of effluent discharges of the petroleum industry, there is an imminent need to monitor, constantly, the hydric resources that are influenced by this industrial activity, so that it can be ensured the environmental health. This study aimed, mainly, to analyze the quality of water samples derived from refinement process performed of the largest petroleum refinery in Brazil (REPLAN), in distinct steps of the industry treatment, and waters of rivers associated to its activity. Micronuclei and Nuclear Abnormalities tests were performed in erythrocytes of the fish species Oreochromis niloticus exposed to water samples derived from refinery use and to rivers under the influence of their effluents, in order to evaluate its possible toxic, genotoxic and mutagenic effects. The results obtained show that the treatment carried out by the refinery, during the evaluated periods, was effective, since the substances present in the water samples did not induce significant genotoxic and/or mutagenic alterations in the genetic material of the test organisms. On the other hand, when the substances present in the refinery effluent mix with the ones derived from other industrial activities developed upstream of this industry, the resultant compound, in some periods, presented genotoxic potencial, characterized by a rise of erythrocytic...

Estudo dos mixozoários (Myxozoa : Myxosporea) parasitos dos peixes characiformes Acestrorhynchus lacustris (Lütken, 1875) e Steindachnerina insculpta (Fernández-Yépez, 1948) coletados no Rio do Peixe, médio Rio Tietê, SP, Brasil

The São Paulo State has as one of its main rivers the Tietê, which is an extremely important source because of its biodiversity, use in navigation and mainly as direct supplier of water for thousands of people in the region. Currently, the biodiversity of marine and freshwater ecosystems has been threatened, primarily by environmental problems resulting from the degradation of ecosystems, an example is the stretch of the Middle Tietê River, where the Barra Bonita dam (SP) focused virtually the whole load organic effluents from the metropolitan region of São Paulo, leading to eutrophication with the loss of water quality. This basin is located in the Peixe’s River, a tributary of the Barra Bonita whose sources are on the slopes of the Cuesta de Botucatu, presenting an altitudinal gradient in a good state of conservation and water quality. From another perspective, the studies related to parasites and other pathogens of aquatic organisms have considerably increased the relevance, especially those hosts with potential for growing and marketing, given the significant increase these activities in Brazil and worldwide. Among the agents responsible for diseases in fish are the myxosporean, which can cause major economic damage, with high mortality ratios. Based on the above, this study evaluated the biodiversity of myxosporean parasites of two species of fish Acestrorhynchus lacustris and Steindachnerina insculpta. During the period January to November 2012, 40 specimens of Acestrorhynchus lacustris and 33 specimens of Steindachnerina insculpta were collected from the Peoxe’s river at four different points. The necropsies were performed and all organs were analyzed looking for myxosporean. Only the species Steindachnerina insculpta had their gills parasitized by two species of the genus Henneguya

Produção e aplicação de ramnolipídios produzidos por Pseudomonas aeruginosa LBI 2A1

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Uso de sistemas construídos de áreas alagadas (Constructed Wetland System - CWS) tipo ascendente/descendente, para tratamento de efluentes avícolas

Human development and population growth during the twentieth century increased the water demand, tripling its consumption between 1950 and 1990. As the water streams were polluted; and as water is the source of minerals and also regulates vital functions, it becomes the vehicle of transmission and consequently spreads many diseases. Probably, the industries are the major responsible for this pollution when they dump untreated effluents to water streams, saturating the already insufficient net of sanitation facilities polluting water and soil. An effective treatment has been established with low cost in Europe and the United States, through constructed systems on wetlands Constructed Wetland Systems - CWSs, gradually used in other countries in the last three decades. Lately, we observe a continuous growth in Brazilian poultry business, and poultry industry showed greatest dynamism in the country, following the global market. Pondering this information and the efficiency of such treatment, this work aimed to study prototypes, in a laboratory scale, simulating ascending and descending types of CWSs, vegetated with aquatic macrophytes Eichhornia crassipes and the uses of aggregates and soil, to treat industrial wastewater from slaughterhouses and aviary. We conducted the initial characterization of the effluent to have an idea of its constituents and to scale the system and the continuous flow. Furthermore, we characterized the soil to be used in this system. The collects are periodically made in the refrigeration industry FRICOCK FRIGORIFICAÇÃO AVICULTURA INDÚSTRIA E COMÉRCIO LTDA. for local treatment simulation. The effluent that was treated with 12 prototypes of CWSs are analyzed with some frequency. The results of these reviews were compared to the effluent coming from the industry... (Complete abstract click electronic access below)

Avaliação da auto-suficiência energética de reatores anaeróbios no tratamento de esgoto sanitário e água cinza

The anaerobic treatment of sewage is widely employed in Brazil and it is an appreciated way for the treatment of effluents, helping to reduce the environmental impact in rivers. The methane gas obtained from the process can be applied to improve the energetic efficiency of the system, reducing the amount of waste and the cost of the treatment process. This work presents the net energy balance of anaerobic reactors applied to the treatment of sewage. The analysis was performed considering full-scale and laboratory-scale treatment systems. In laboratory scale, the results from three kinds of systems were compared regarding the biological treatment of greywater. Two of them (UASB7 and UASB12) were anaerobic and the other one was a combined anaerobic-aerobic system (UASB7/SBR6). Greywater methanization (compared to theoretical maximum) was calculated considering 100% removal (g BOD/day), the literature percentage removal and the anionic surfactant presence in the effluentt. For each of these three cases, the efficiencies were, respectively, 16.9%, 43.6% and 51.3% in UASB7 reactor, 25.6%, 50.3% and 59.2% in UASB12 reactor and 30.6%, 61.2% and 71.9% in UASB7/SBR6 reactor. The energetic potential was found to be 4.66x10-4, 7.77x10-4 and 5.12x10-4 kWh/L for the UASB7, UASB12 and UASB7/SBR6 reactors, respectively. The pumping system, the aeration (in the anaerobic-aerobic system) and the temperature controlled heating system were considered to calculate the energetic consumption. However, the third one was not employed since tropical regions like Brazil do not need heating systems and also because of its high energetic consumption. The calculated net energy balance in the reactors was negative in the case of greywater, respectively -0.16, -0.28 and -0.18 kWh/L for the reactors UASB7, UASB12 and UASB7/SRB6. In full scale (ETE Jardim das Flores - Rio Claro, SP), the average energy... (Complete abstract click electronic access below)

Projeto de um sistema anaeróbico para estabilização de lodo e recuperação de energia

The treatment of domestic and industrial effluents through Wastewater Treatment Plants (WTPs) generates a residue termed sewage sludge, rich in organic matter, high-volume, occasionally containing pathogens and heavy metals. The sludge generation can minimize the benefits brought by the treatment of sewage, because this residue does not always receive appropriate treatment before final disposal. The disposal is another problem related to sludge. Landfills generally does not have physical space and alternatives such as the use in agriculture requires an intense treatment that could be in many cases operational or economic unfeasible. The objective of this work is the theoretical research about the processes of stabilization of the sludge by anaerobic digestion and the methanogenic activity during the process. Through analysis of each step and contemplating each relevant factor in anaerobic digestion process in order to optimize them, we proposed a theoretical model of reactors capable of stabilize the sludge, reduce its volume and eliminate pathogens. The obtained configuration consists of two anaerobic reactors connected in series. The first one operates in the range mesophilic temperature (35 ° C) and has higher hydraulic retention time (25 days) working primarily in the stabilization of organic matter present in the sludge and producing biogas, whereas the second one operates in the thermophilic range (55 ° C) in order to eliminate pathogens, and to reduce the volume. The hydraulic retention time in the second reactor is lower (10 days). Both mesophilic and thermophilic processes were efficient in what was proposed, promoting the stabilization of organic matter present in the sludge and significant reduction of pathogens. As a final step with the sludge previously digested, it is indicated a final dehydration... (Complete abstract click electronic access below)

Remoção de matéria orgânica e nitrogênio de efluentes provenientes de abatedouros de bovinos por meio de reator aeróbio operando em bateladas sequenciais com adição de coagulante químico

The volume of liquid effluent generated in cattle slaughterhouses is quite high and cannot be released untreated in water bodies due to its high pollution load of predominantly organic origin. To minimize the environmental impacts of its industrial wastewater and meet the local environmental legislation, abattoirs shall make the treatment of these effluents. The present work aims to develop the study of a reactor by sequential batch pilot scale, in order to optimize their performance in treating wastewater from a cattle slaughterhouse. The treatment system used was developed and installed in the Laboratory of Wastewater Treatment, in Faculty of Science and Technology UNESP, Presidente Prudente campus. The procedure used followed the operation of sequential batch reactors, in which all processes and treatment operations occurring sequentially in a single unit, by establishing specific operating cycles, which comprise the following separated phases: aerobic reaction, anoxic reaction, sedimentation and emptying. Aiming to improve the quality of treatment was planned the addition of coagulant Poly Aluminum Chloride (PAC) in the reactor, by determining their optimal dosage by Jar-test trials. Were prepared four steps with specific operating cycles: step one or acclimatization (10 hour of aeration, one hour and 30 minutes of sedimentation and 30 minutes for exchanging the effluent); step 2 (6 hours of aeration or aerobic phase, 4 hours and 45 minutes of stirring or anoxic phase and 1 hour and 15 minutes for sedimentation and exchange effluent); step 3 (2 hours and 30 minutes of aeration, 8 hours and 15 minutes of stirring and 1 hour and 15 minutes for sedimentation and exchange) and step 4 (2 hours of aeration, 8 hours and 45 minutes of stirring and 1 hour and 15 minutes for sedimentation and exchange)... (Complete abstract click electronic access below)

Toxicidade de petróleo bruto sobre microcrustáceo Daphnia similis em água de rio e água de produção de petróleo

Aquatic ecosystems are suffering many impacts caused by human activities resulting from the activities occurring around them. With technological progress observed in recent years, this environment has received large amounts of chemicals from industries, agriculture and urban area that affect the aquatic biota. Among these sources of contamination, the oil industry has contributed to the pollution of aquatic environments with both effluents as produced water well as oil spills and their derivatives having toxicity to various organisms. With all the environmental issues has increased concern about water quality and has been used ecotoxicological tests with aquatic organisms to ecosystems to assess the toxicity of chemicals present in the water. In this context the microcrustacea Daphnia similis stands out as a freshwater organism very representative of the aquatic fauna of rivers and high sensitivity to environmental impacts. Thus, the present study aimed to evaluate the lethal toxicity of crude oil and produced water on this microcrustacea. The results showed that the microcrustacea presented high sensitivity to contaminants primarily crude oil. There was also the influence of environmental variables pH and temperature on the survival of organisms

Comparação de eficiências do uso de sistemas construídos de áreas alagadas (Constructed Wetland Systems - CWS), tipos ascendente e descendente, para tratamento de efluentes

Water is an essential element for life. The use of this element, to support the community, defines it as water resource. This feature is being misused and degraded by the dumping of highly contaminated effluents. The impoverishment of its quality poses a risk to human consumption. The necessity to manage this resource, treating the wastewater properly, requires the constant improvement of treatment systems. Another need is to adjust the cost of systems to the demands of communities with less financial clout. This study aimed to adapt and understand the systems of wetlands, improving its efficiency, in an attempt to collaborate with the enrichment of this technology. The practical evidence, with lab-scale prototypes, assembled in ETE Piracicamirim with urban sewage effluent contributed to highlight the problems and operating system design. The bibliographic review showed that several studies had effectiveness for treatment. But it was evident the need for better understanding of dimensioning definitions that better attempted to the answers into the project. Moreover, standardization of system conditions for the specific wastewater treatment is an interesting field, identified, for future studies yet contribute to environmental engineering and sanitation

Mapeamento geoestatístico da vulnerabilidade do Aquífero Guarani no município de Ribeirão Bonito - SP

The Guaraní aquifer has relevant importance both as a source of water for several urban centres and the development of agriculture and livestock. In recharge areas the aquifer is free and, therefore, subject to contamination of effluents and tailings deposited on soils that cover it. Thus, it becomes crucial not only its protection at all levels, as the knowledge of its degree of natural vulnerability. The present work used geostatistics modeling techniques to study the natural vulnerability of the Guaraní aquifer in the city of Rio Bonito, State of São Paulo, southeastern Brazil, where the Guarani aquifer is exposed. These techniques, extensively used in evaluation studies of mineral deposits and oil tanks, can be adapted to produce a spatial classification or a regionalisation of probabilistic indices of vulnerability. By ordinary kriging method maps of vulnerability classification were obtained. To determine the vulnerability of the aquifer was employed the Aquifer Vulnerability Index (AVI), which requires knowledge of unsaturated zone thickness and permeability. The final product was a map with probabilistic index of vulnerability of the Guaraní aquifer, which presented values between 0 to 0.33 years, framing the area studied in AVI class extremely high vulnerability

Desidratação de lodo proveniente de ETA através de tubos geotexteis

Currently exists a growing concern for the preservation of the environment Around the world, the environmental awareness in Brazil has strengthened during the past two decades. This concern in Brazil arises from the creation of mechanisms of supervision and punishment on the part of the environmental agencies. In order to meet the pertinent legislation many public and private companies have performed dehydration of waste generated in the process of treatment of industrial effluents, waste water and water treatment in order to reduce transport and disposal costs. The use of geotextile tubes has proven technically and economically feasible to be applied in various situations from water treatment to mining tailings. This work presents the solution adopted for an environmental liability in water treatment plant through the use of geotextile tubes to reduce the water content of the disposal. We evaluated the size distribution curves, Atterberg limits, and chemical composition of the residue. Found high concentrations of aluminum in the waste which would characterize environmental pollution if disposed off directly in nature with no treatment

Tratamento de efluentes provenientes de abatedouro de bovinos por meio de reator enzimático seguido de reator aeróbio operado em bateladas sequenciais

Brazil has one of the largest cattle herds in the world, so the cattle slaughter is one of the most important economic activities in the Brazilian market. But this activity requires a high demand of water, resulting in serious problems about the correct disposal of wastewater generated in the process. This effluent has a high pollution load, becoming its receiving bodies (streams and rivers) unfit for various activities such as public water supply, recreation, fisheries. To minimize the environmental impacts of its industrial wastewater and fallow the local environmental legislation, refrigerators must make the treatment of these effluents. This study aimed to verify the efficiency of a enzymatic reactor, when occur hydrolysis of lipids present in the effluent industrial of an cattle slaughter industry. The treatment system used was composed of two separate reactors: one being the anaerobic fluidized bed reactor (AFBR), inoculated with immobilized enzymes on the matrix support, and the other by sequential batch reactor (SBR) inoculated with activated sludge. Whereas, the reactors have been developed and installed at the Wastewater Treatment Laboratory, Faculdade de Ciências e Tecnologia, UNESP, campus Presidente Prudente. The procedure operating occurred differently for each reactor: preparation and inoculation of enzyme granules, filling the reactor, hydrolysis, and AFBR emptying, filling, aerobic reaction, sedimentation, and emptying the SBR. We performed three experimental stages, with the first and second stage of the work were done reactor analyzes separately, and the third step of the analysis were made with the interconnected reactors... (Complete abstract electronic access below)

Avaliação da membrana P81 como agente ligante da técnica de difusão em filmes finos por gradiente de concentração (DGT) para determinação de mercúrio em sistemas aquáticos

Worldwide environmental degradation is an undesirable byproduct resulting from the increasing demand for natural resources. Water sources are suffering intense contamination since they usually receive a huge amount of domestic and industrial effluents - which are mostly wasted without proper treatment - inserting a large number of pollutants in the environment, heavy metals included. Mercury holds great toxicological importance because, under some physicochemical conditions in a water environment, Hg (II) ion turns into methylated compounds stemming from this element, such as methylmercury CH3Hg, which is highly toxic for the aquatic community in which bioaccumulation occurs. Nowadays passive sampling techniques are being developed to enable the analytical procedures which are applied in environmental monitoring. Diffusive gradients in thin-films technique (DGT) has been proven an interesting tool for the determination of labile metal species due to its in situ application. The DGT technique consists of a piston-like device on which the following series of agents is disposed: a binding agent (conventionally Chelex 100 resin), a diffusive agent, usually a polyacrylamide gel, and a membrane filter. Nevertheless, the agents conventinally used for this technique don't usually show satisfactory results in mercury sampling. The main goal of this study was to evaluate the phosphate-treated cellulose membrane (Whatman P 81), an alternative material, as binding agent in the DGT to determine labile mercury fractions in aquatic systems. In this context, we conducted a study of the behavior of this material in relation with system variables, pH and ionic strength. Afterwards we performed immersions of the DGT devices in real and enriched samples and in situ aiming the determination of mercury

Study of the removal of chemical oxygen demand of the cheese whey and wastewater from dairy industry using Spirulina platensis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Characterization of immobilized biomass by amplified rDNA restriction analysis (ARDRA) in an anaerobic sequencing-batch biofilm reactor (ASBBR) for the treatment of industrial wastewater

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)